Antiviral Property of the Fungal Metabolite 3-O-Methylfunicone in Bovine Herpesvirus 1 Infection

Bovine herpesvirus type-1 (BoHV-1) is a widespread pathogen that provokes infectious rhinotracheitis and polymicrobial infections in cattle, resulting in serious economic losses to the farm animal industry and trade restrictions. To date, non-toxic active drugs against BoHV-1 are not available. The exploitation of bioactive properties of microbial products is of great pharmaceutical interest. In fact, fungi are a promising source of novel drugs with a broad spectrum of activities and functions, including antiviral properties. Hence, the potential antiviral properties of 3-O-methylfunicone (OMF), a secondary metabolite produced by Talaromyces pinophilus, were evaluated on BoHV-1. In this study, during BoHV-1 infection in bovine cells (MDBK), the non-toxic concentration of 5 µM OMF considerably reduced signs of cell death and increased cell proliferation. Furthermore, OMF significantly decreased the virus titer as well as the cytopathic effect and strongly inhibited the expression of bICP0, the major regulatory protein in the BoHV-1 lytic cycle. These findings were accompanied by a considerable up-regulation in the expression of the aryl hydrocarbon receptor (AhR), a multifunctional transcription factor also linked to the host’s response to a herpesvirus infection. Overall, our results suggest that by involving AhR, OMF shows potential against a BoHV-1 infection.

Prevention and Control of African Swine Fever in the Smallholder Pig Value Chain in Northern Uganda: Thematic Analysis of Stakeholders' Perceptions

African swine fever (ASF) is endemic in Uganda and considered a major constraint to pig production. In the absence of a vaccine, biosecurity is key for ASF prevention and control. To improve prevention and control on farm and community level there is need for more knowledge on current application of biosecurity practises, and better understanding of how pig value chain actors perceive prevention and control. To achieve this, a qualitative interview study involving focus group discussions (FGD) was conducted with actors from the smallholder pig value chain in northern Uganda. Six villages were purposively selected based on previous outbreaks of ASF, preliminary perceived willingness to control ASF, and the representation of several different value chain actors in the village. Results indicated that biosecurity practises such as basic hygiene routines including safe carcass handling, minimising direct and indirect contacts between pigs or between pigs and people, trade restrictions and sharing of disease information were implemented in some of the villages. Thematic analysis based on grounded theory revealed six categories of data relating to ASF prevention and control. Together these categories form a logical framework including both enablers and hindrances for ASF prevention and control. In summary participants mostly had positive perceptions of ASF biosecurity, describing measures as effective. Participants further possessed knowledge of ASF and its transmission, some of which was in line with known scientific knowledge and some not. Nevertheless, participants were hindered from preventing and controlling ASF due to biosecurity costs and a need to prioritise family livelihood over disease transmission risks, incompatibility of current biosecurity practises with local culture, traditions and social contexts and finally lack of access to veterinarians or, occasionally, low-quality veterinary services. The constraints could be addressed by applying participatory processes in designing biosecurity measures to ensure better adaptation to local cultural and social contexts.

Characterization of a Nigerian Lumpy Skin Disease Virus Isolate after Experimental Infection of Cattle

Lumpy skin disease virus (LSDV), together with sheeppox virus and goatpox virus, belong to the genus Capripoxvirus within the family Poxviridae. Collectively, they are considered the most serious poxvirus diseases of agricultural livestock. Due to their severe clinical course and consequent loss of production, as well as high mortality of naïve small and large ruminant populations, they are known to have a significant impact on the economy and global trade restrictions of affected countries. Therefore, all capripox diseases are classified as notifiable under the guidelines of the World Organization of Animal Health (OIE). Since the 1970s, several outbreaks of LSD have been recorded in Nigeria. Until now, only a little information on the virus strains leading to the reported outbreaks have been published, dealing mainly with the phylogenetic relationship of those strains and the description of field outbreaks. During the present study, we experimentally infected cattle with a low-passage Nigerian LSDV strain isolated from a skin sample of LSD positive cattle in Nigeria in 2018. Clinical, molecular and serological data indicate that this LSDV isolate is highly pathogenic in cattle since it induced a severe clinical course and approximately 33% mortality in naïve Holstein Friesian cattle after experimental infection.

Paratuberculosis: The Hidden Killer of Small Ruminants

Paratuberculosis (PTB) is a contagious and chronic enteric disease of ruminants and many non-ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP), and is characterised by diarrhoea and progressive emaciation with consequent serious economic losses due to death, early culling, and reduced productivity. In addition, indirect economic losses may arise from trade restrictions. Besides being a production limiting disease, PTB is a potential zoonosis; MAP has been isolated from Crohn’s disease patients and was associated with other human diseases, such as rheumatoid arthritis, Hashimoto’s thyroiditis, Type 1 diabetes, and multiple sclerosis. Paratuberculosis in sheep and goats may be globally distributed though information on the prevalence and economic impact in many developing countries seem to be scanty. Goats are more susceptible to infection than sheep and both species are likely to develop the clinical disease. Ingestion of feed and water contaminated with faeces of MAP-positive animals is the common route of infection, which then spreads horizontally and vertically. In African countries, PTB has been described as a “neglected disease”, and in small ruminants, which support the livelihood of people in rural areas and poor communities, the disease was rarely reported. Prevention and control of small ruminants’ PTB is difficult because diagnostic assays demonstrate poor sensitivity early in the disease process, in addition to the difficulties in identifying subclinically infected animals. Further studies are needed to provide more insight on molecular epidemiology, transmission, and impact on other animals or humans, socio-economic aspects, prevention and control of small ruminant PTB.

Behavioral Analysis in International Business Negotiations Based on the Bargaining Model of Game Theory

In the process of global digital trade rules negotiation, game and cooperation coexist, and all parties are actively seeking cooperative relationship while negotiating game. Against this background, this paper provides a comprehensive overview of the negotiation process of global digital trade rules and analyzes the core issues of the negotiations and their implied political game. This paper firstly compares the core issues of global digital trade rules negotiations at multilateral and regional levels and quantitatively reflects the weighting relationship between core issues and keywords by means of a dynamic thematic model. In order to judge the potential partnership of each country, this paper constructs a theoretical model of partner selection for global digital trade rules negotiation from the perspective of political game and uses the data of 62 major digital trade countries for empirical testing. Compared with traditional trade agreements, the consensus reached in digital trade negotiations is less influenced by traditional economic geography factors and more influenced by the level of digital trade restrictions and political distance. When signing digital trade terms, a country tends to choose countries with similar levels of digital trade restrictions and high political similarity as partners. Financial restrictions and trade restrictions are the most important digital trade restriction factors that countries pay attention to when negotiating, and political stability is the most important political factor that countries pay attention to when negotiating. Compared with developed countries, developing countries pay more attention to market size, cultural differences, and digital infrastructure when negotiating. The formulation of global digital trade rules may follow a reconstructive path from bilateral to regional to multilateral, promoting regional digital trade rule systems at this stage and gradually expanding the scope of negotiations to the multilateral level.

Global Trade and Cybersecurity

Trade in information and communications technologies (ICTs) is a new currency of international power. On the one hand, this commerce boosts national prosperity and the global economy. On the other hand, it puts capabilities for harm into the hands of potential malicious actors or political adversaries. States are scrambling to introduce new security control mechanisms through a variety of laws, regulations, and policies. These practices will have mixed results, not least because some of the more threatening elements of the trade relate to software products and even the results of basic scientific research that cannot be tracked as easily as physical goods. Enforcement of trade restrictions in this area will remain problematic. Even so, states are looking to undertake economic sanctions in this area to retaliate for the malicious behaviour in cyberspace of other states (or of criminals operating with impunity from the territory of other states). The corporate sector has emerged as an independent actor in these interstate controversies, and they have been joined by scientists and civil society activists, all pressing for a more liberal and open approach. While the unilateralist trend seems to have the upper hand for now, common interests and the imperatives of trade may force new collaborative behaviours among states.

Corn trade simulations of China: reduction in tariffs versus expansion in tariff-rate quotas

PurposeCorn, which has the highest domestic production, planting area and consumption, is the top cereal in relation to demand and supply in China. However, the comparative advantage of China in corn has continuously deteriorated in recent years and based on the recent situation and possible supply and demand trends, it is widely accepted that a corn self-sufficiency rate of 95% is difficult to achieve. Under current import-restriction policies, corn may stand at the crossroads of reforms to solve its predicted insufficient supply. In this study, the authors analyse the necessity of relaxing trade restrictions on corn in China and explore the effects of trade restrictions by reducing tariffs and expanding tariff-rate quotas on corn and related industries and the welfare change caused by possible relaxations.Design/methodology/approachThe authors construct a computable general equilibrium (CGE) model and design nine scenarios for the analysis.FindingsThe results show that relaxations of import restrictions are probable methods to meet the aim of sufficient corn supply during shortages. They are simulated to reduce corn's domestic production and price, increase import and import prices and lead to a decline in self-sufficiency but benefit the production of corn-related industries of corn. The results also imply that expanding the quota is a better method for releasing trade restrictions in China.Originality/valueThe comparative advantage of China in corn deteriorated with an increase in prices. Based on the current situation and possible trends of supply and demand, the referenced goal of achieving 95% corn self-sufficiency appears difficult, implying that reliance on imports is probably imminent and vital. This study provides simulation results in future scenarios and offers policy implications for China's corn trade policies.

A Multi-Phase Assessment of the Effects of COVID-19 on Food Systems and Rural Livelihoods in Ethiopia: The Case of Fogera Plain

The COVID-19 pandemic has not only led to the loss of human life and resulted in an unprecedented challenge to public health, but has also seriously affected food systems and work opportunities. As a global pandemic, COVID-19 has impacted food systems and livelihoods as a result of both economic and health challenges that emanate from domestic public policy measures, and also actions taken by other countries, mainly in the form of trade restrictions. Following the confirmation of the first COVID-19 case in Ethiopia on 13 March 2020, and concerns about the sharp increase in cases, the federal government declared a state of emergency on 8 April 2020 which lasted for five months. This paper presents the assessment of the impacts of the COVID-19 pandemic and its prevention measures on agricultural commercialisation, food and nutrition security, labour and employment, as well as poverty and well-being in rural Ethiopia.

The Growing Role of Re-export in International Trade

The article aims to determine the essence, role, and significance of re-export in international trade using its opportunities to develop Russian exports and imports.The authors define re-export and its key characteristics, analyze the main factors of re-export trade development, study re-export dynamics compared to the commodity supplies dynamics within the framework of ordinary exports. When exporting highly differentiated goods, it is the role of knowledge about the terms, forms, and trading methods on the international market that increases. Specialized trading companies possess this knowledge; they create large transport and logistics centers in the countries crossing international trade routes, including extensive consignment warehouses.The article studies the dynamics of re-export flows and makes calculations, based on which it concludes that the volume of these commercial operations is growing faster in the world market. At the same time, large re-export operations are now performed not only by small states located at the intersection of sea roads but also by large, industrialized states: the USA, Great Britain, Italy, etc.The authors conclude that an essential factor in the development of re-export was the growth of trade restrictions imposed primarily for political reasons. Such restrictions apply in trade relations between India and Pakistan, Israel and the Arab countries, between some states of the Persian Gulf, etc. The anti-Russian sanctions adopted in the United States and other Western states inadvertently contribute to the development of re-exports, since many trading companies, including Western ones conducting international commercial operations, do not consider themselves bound by unilateral decisions of the anti-Russian part of the US government. Many US trading companies themselves are actively involved in re-export operations without disclosing partners. Given the undesirability of disseminating information on re-export operations, many countries actively involved in re-export have stopped submitting data on their re-exports to international statistical agencies.The authors concentrate on the re-export operation of the United Arab Emirates, where are thousands of trading firms with transport and logistics infrastructure and consignment warehouses. Russia is involved in re-export trade, mainly through the UAE companies, carrying on export and import operations on the world market. The authors conclude that expanding the practice of re-export increases sales of national products in the world market.