Comparison of Serum Dynamic Thiol/Disulfide Ratios in Low and High Grade Cervical Preinvasive Lesions

Abstract Purpose: This study aimed to determine the potential clinical use of dynamic thiol disulfide balance in cases with preinvasive lesions of the cervix.Methods: One hundred and sixteen patients with high-grade squamous intraepithelial lesion, one hundred patients with low-grade squamous intraepithelial lesion and one hundred and ten healthy controls were enrolled in the study. A fully automated colorimetric system was used to determine the levels of thiol-disulfide parameters. The ischemia-modified albumin, total oxidant-antioxidant capacity, oxidative stress index of the retrieved cases were further analysed.Results: Native thiol and total thiol levels are significantly lower in the high-grade squamous intraepithelial lesion group according to control group (p:0.004 and p:0.015, respectively). Disulfide level is significantly increased in the high-grade squamous intraepithelial lesion group compared to control group (p:0.004). Oxidative stress index levels in high-grade squamous intraepithelial lesion group were observed as significantly higher according to the control group (p:0.014). Ischemia-modified albumin levels in the high-grade squamous intraepithelial lesion group were observed as significantly higher compared to the control group (p:0.020). Disulfide levels are positively correlated with risk type of Human papillomavirus (r:0.420, p<0.001).Conclusion: The analysis of dynamic thiol disulfide balance revealed considerable oxidative damage in patients with Human papillomavirus -related cervical precursor lesions compared to women with ordinary cytology specimens. Therefore, investigation of thiol disulfide balance with presented method represents a new promising test for early diagnosis and management of women at high risk for cervical cancer.

Download Full-text

Changes in the Extracellular Matrix Are Associated With the Development of Serous Tubal Intraepithelial Carcinoma Into High-Grade Serous Carcinoma

International Journal of Gynecological Cancer ◽

10.1097/igc.0000000000000933 ◽

2017 ◽

Vol 27 (6) ◽

pp. 1072-1081 ◽

Cited By ~ 4

Author(s):

Sophieke C.H.A. van der Steen ◽

Johan Bulten ◽

Koen K. Van de Vijver ◽

Toin H. van Kuppevelt ◽

Leon F.A.G. Massuger

Keyword(s):

Extracellular Matrix ◽

Cancer Progression ◽

Serous Carcinoma ◽

High Grade ◽

Full Spectrum ◽

Preinvasive Lesions ◽

Intraepithelial Carcinoma ◽

Screening And Prevention ◽

Step Transition ◽

Intraepithelial Lesions

ObjectiveThe identification of a marker for early progression of preinvasive lesions into invasive pelvic high-grade serous carcinoma (HGSC) may provide novel handles for innovative screening and prevention strategies. The interplay between cancer cells and the extracellular matrix (ECM) is one of the main principles in cancer development and growth, but has been largely neglected in preinvasive lesions. This is the first study addressing the involvement of the ECM in the “step-by-step” transition of normal fallopian tube epithelium into preinvasive lesions, and eventually the progression of preinvasive lesions into invasive HGSC.MethodsThe expression of highly sulfated chondroitin sulfate (CS-E), a characteristic glycosaminoglycan of the cancer-associated ECM, was assessed by immunohistochemistry in a large cohort of precursor lesions of the full spectrum of HGSC development, including 97 serous tubal intraepithelial carcinomas (STICs), 27 serous tubal intraepithelial lesions, and 24 p53 signatures. In addition, the immunological reactivity in the microenvironment was evaluated.ResultsIncreased stromal expression of highly sulfated CS-E was observed in 3.7%, 57.7%, and 90.6% of serous tubal intraepithelial lesions, STICs, and invasive HGSCs, respectively (P < 0.001). No or limited expression was found in p53 signatures and normal tubal epithelium (compared with STIC, P < 0.001). A gradual increase in the amount of CS-E expression between STIC and paired HGSC was demonstrated. Intense stromal CS-E expression in STIC was significantly associated with an immune infiltrate (P < 0.001).ConclusionsOur study showed that increased stromal CS-E expression is related to the degree of the tubal epithelium abnormality. Specific alterations in the ECM (ie, CS-E expression) occur early in pelvic HGSC development and may represent a novel biomarker of early cancer progression, useful for the identification of novel clinical strategies.

Download Full-text

Are all high-grade preinvasive lesions premalignant, and should they all be treated?

American Journal of Respiratory and Critical Care Medicine ◽

10.1164/ajrccm.165.10.165105 ◽

2002 ◽

Vol 165 (10) ◽

pp. 1452-1453 ◽

Cited By ~ 5

Author(s):

Anindo K. Banerjee ◽

Pamela H. Rabbitts ◽

P. Jeremy M. George

Keyword(s):

High Grade ◽

Preinvasive Lesions

Download Full-text

High-risk human papillomavirus, other than type 16/18, in predominantly older Taiwanese women with high-grade cervical preinvasive lesions

Taiwanese Journal of Obstetrics and Gynecology ◽

10.1016/j.tjog.2013.04.012 ◽

2013 ◽

Vol 52 (2) ◽

pp. 222-226 ◽

Cited By ~ 6

Author(s):

Ying-Cheng Chiang ◽

Wen-Fang Cheng ◽

Yu-Li Chen ◽

Ming-Cheng Chang ◽

Chang-Yao Hsieh ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

High Grade ◽

Taiwanese Women ◽

Preinvasive Lesions

Download Full-text

Concurrent cytologic and colposcopic evaluation of symptomatic cervical erosion

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20215088 ◽

2021 ◽

Vol 11 (1) ◽

pp. 125

Author(s):

Shreya Goel ◽

Prabha Lal

Keyword(s):

Screening Tool ◽

Cervical Dysplasia ◽

Latency Period ◽

High Grade ◽

High Grade Lesion ◽

Long Latency ◽

Preinvasive Lesions ◽

Sensitivity Specificity ◽

Histopathological Results ◽

Causes Of Deaths

Background: Cervical cancer is amongst the leading causes of deaths due to cancer in developing countries. Moreover, preinvasive lesions of the cervix have a long latency period for conversion into malignancy and are also detectable by screening techniques. Hence, colposcopy in addition to cytology should be carried out wherever facility is available to ensure early detection and timely management.Methods: Simultaneous cytology and colposcopy was done for 80 women with symptomatic cervical erosion followed by a colposcopic directed biopsy in women with MRCI >3. Finally, correlation between cytology, colposcopy and histopathological results was done.Results: 65/80 women were biopsied. 12/80 women had MRCI >6 amongst which 10/80 were confirmed to have a high grade lesion on histopathology. 13/80 had lesser abnormalities (ASCUS and LSIL) amongst which 3/80 had CIN1 on histopathology. Only 2/80 had HSIL on cytology as compared to 8/80 on histopathology that had CIN 2/3. Lastly, only 1/80 had SCC on cytology compared to 2/80 on histopathology. The sensitivity, specificity, PPV and NPV of cytology and colposcopy for diagnosing cervical dysplasia was 46.1%, 83.5%, 35.2%, 88.8% and 84.6%, 86.5%, 55%, 96.6% respectively making colposcopy a better screening tool than cytology for evaluating cervical malignancy.Conclusions: Colposcopic examination should ideally be carried out in all women with symptomatic cervical erosion in addition to cytology. Moreover, suspicious areas should be biopsied even if cytology is normal to exclude malignancy.

Download Full-text