scholarly journals Polimorfisme Protein Darah Itik Pegagan dengan Metode PAGE

2011 ◽  
Vol 11 (2) ◽  
pp. 58-62
Author(s):  
Meisji Liana Sari ◽  
Ronny Rachman Noor ◽  
Peni S. Hardjosworo ◽  
Chairun Nisa
Keyword(s):  
Genetic Diversity ◽  
Blood Protein ◽  
Limited Information ◽  
Genetic Characteristic ◽  
Duck Species ◽  
Protein Locus ◽  
Electrophoresis Method

polymorphism of blood protein pegagan duck by PAGE methodABSTRACT. Pegagan duck is one of local duck species which comes from Ogan Ilir (OI) Regency, South Sumatera. This species is not popular, yet. But, it has spread out along Ogan river which comprises of three districts e.g Tanjung Raja, Inderalaya and Pemulutan. The ecosystem in this three districts is dominated by swampy area. As far, there is relatively limited information of Pegagan duck compared to another local duck. So, for the first step was done a reseach to identify genetic characteristic from blood protein analyses by electrophoresis method. It is expected that this information will become the bases in determining of the policy of Pegagan duck development. Polymorphism analyses of blood protein was done with 9 duck samples which taken randomly from 400 pegagan ducks. Protein locus observed were albumin (Alb), post albumin (Pa), transferrine (Tf), post transferrine-1 (Ptf-1), and post transferrine-2 (Ptf-2). Polymorphism analyses of blood protein of pegagan duck was characterized by allele A in locus Transferrine (TfA), Post transferrine-1 (Ptf-1A) and Post transferrine-2 (Ptf-2A) . Genetic diversity based on blood protein of Pegagan duck showed that gen frequency value in a range of 0,11 – 0,94 and high heterozygosis value that was 0,62 %.

scholarly journals Keragaman genotipik entok (Cairina moschata) berdasarkan polimorfisme protein darah

2021 ◽  
Vol 19 (3) ◽  
pp. 247
Author(s):  
Sutopo Sutopo ◽  
Dela Ayu Lestari ◽  
Asep Setiaji ◽  
Edy Kurnianto
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Gene Frequency ◽  
Blood Protein ◽  
Muscovy Duck ◽  
Central Java ◽  
Cairina Moschata ◽  
Electrophoresis Method ◽  
Muscovy Ducks ◽  
I Gene

<p class="MDPI17abstract"><strong>Objective: </strong>The aim of this study was to identified genetic diversity of muscovy duck in Central Java through blood protein polymorphisms by using electrophoresis method.</p><p class="MDPI17abstract"><strong>Methods: </strong>Blood sample was collected from a total of 60 muscovy ducks from the districts of Demak, Magelang and Pekalongan, 20 samples each. The Electrophoresis Polyacrylamide Gel of Electrophoresis–Thin Layer Electrophoresis (PAGE-TLE) was used in this study. Parameters observed were protein of albumin (Alb), ceruloplasmin (Cp), transferrin (Tf) and amylase-I (Am-I). Gene frequency, heterozygosity (Ho) and genetic distance were analyzed by using DISPAN program.<strong></strong></p><p class="MDPI17abstract"><strong>Results</strong><strong>: </strong>The results showed that the Alb, Cp, Tf and Am-I of muscovy duck showing polymorphic characters. Gene frequency of Alb<sup>B</sup> (0.61) was higher compared to Alb<sup>A</sup> (0.39). Gene frequency of Cp<sup>A</sup> (0.62) was higher than the Cp<sup>B</sup> (0.38). Furthermore, gene frequency of Tf<sup>A</sup> and Tf<sup>B</sup> were 0.32 and 0.68, respectively as well as gene frequency of Am-I<sup>A</sup> (0.82) was higher than Am-I<sup>B</sup> (0.18). Muscovy duck population of Demak showed higher Ho value for Alb and Am-I, whereas Magelang showed higher value for Cp and Tf. Population of muscovy duck of Demak showed closer genetic distance to Pekalongan than Magelang.<strong></strong></p><strong>Conclusions: </strong>The protein blood locus of three population of muscovy duck in Central Jawa showed polymorphic characters.

scholarly journals Scrub Typhus and Molecular Characterization of Orientia tsutsugamushi from Central Nepal

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 422
Author(s):  
Rajendra Gautam ◽  
Keshab Parajuli ◽  
Mythili Tadepalli ◽  
Stephen Graves ◽  
John Stenos ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Scrub Typhus ◽  
Febrile Illness ◽  
Acute Febrile Illness ◽  
Limited Information ◽  
Orientia Tsutsugamushi ◽  
Health Measures ◽  
Central Nepal ◽  
Vector Borne

Scrub typhus is a vector-borne, acute febrile illness caused by Orientia tsutsugamushi. Scrub typhus continues to be an important but neglected tropical disease in Nepal. Information on this pathogen in Nepal is limited to serological surveys with little information available on molecular methods to detect O. tsutsugamushi. Limited information exists on the genetic diversity of this pathogen. A total of 282 blood samples were obtained from patients with suspected scrub typhus from central Nepal and 84 (30%) were positive for O. tsutsugamushi by 16S rRNA qPCR. Positive samples were further subjected to 56 kDa and 47 kDa molecular typing and molecularly compared to other O. tsutsugamushi strains. Phylogenetic analysis revealed that Nepalese O. tsutsugamushi strains largely cluster together and cluster away from other O. tsutsugamushi strains from Asia and elsewhere. One exception was the sample of Nepal_1, with its partial 56 kDa sequence clustering more closely with non-Nepalese O. tsutsugamushi 56 kDa sequences, potentially indicating that homologous recombination may influence the genetic diversity of strains in this region. Knowledge on the circulating strains in Nepal is important to the development of diagnostic tests and vaccines to support public health measures to control scrub typhus in this country.

scholarly journals Genetic diversity of trypanosome species in tsetse flies (Glossina spp.) in Nigeria

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Judith Sophie Weber ◽  
Sen Claudine Henriette Ngomtcho ◽  
Stephen Saikiu Shaida ◽  
Gloria Dada Chechet ◽  
Thaddeus Terlumun Gbem ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Protected Areas ◽  
National Parks ◽  
Sub Saharan Africa ◽  
Tsetse Flies ◽  
Insect Vector ◽  
Limited Information ◽  
Sub Saharan ◽  
Dynamic Populations

Abstract Background Trypanosomes cause disease in humans and livestock in sub-Saharan Africa and rely on tsetse flies as their main insect vector. Nigeria is the most populous country in Africa; however, only limited information about the occurrence and diversity of trypanosomes circulating in the country is available. Methods Tsetse flies were collected from five different locations in or adjacent to protected areas, i.e. national parks and game reserves, in Nigeria. Proboscis and gut samples were analysed for trypanosome DNA by molecular amplification of the internal transcribed spacer 1 (ITS1) region and part of the trypanosome specific glycosomal glyceraldehyde-3-phosphate dehydrogenase (gGAPDH) gene. Results The most abundant Trypanosoma species found in the tsetse gut was T. grayi, a trypanosome infecting crocodiles. It was ubiquitously distributed throughout the country, accounting for over 90% of all cases involving trypanosomes. Trypanosoma congolense was detected in gut samples from all locations except Cross River National Park, but not in the proboscis, while T. brucei (sensu lato) was not detected at all. In proboscis samples, T. vivax was the most prominent. The sequence diversity of gGAPDH suggests that T. vivax and T. grayi represent genetically diverse species clusters. This implies that they are highly dynamic populations. Conclusions The prevalence of animal pathogenic trypanosomes throughout Nigeria emphasises the role of protected areas as reservoirs for livestock trypanosomes. The genetic diversity observed within T. vivax and T. grayi populations might be an indication for changing pathogenicity or host range and the origin and consequences of this diversity has to be further investigated.

Comparative Analysis of the Genome Sequence of the Polycyclic Aromatic Hydrocarbons (PAHs)-Degrading Bacterium Advenella kashmirensis W13003

2014 ◽  
Vol 955-959 ◽  
pp. 302-305
Author(s):  
Xin Xin Wang ◽  
Chen Li ◽  
Li Bin Zhao ◽  
Liang Wu ◽  
Wei An ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Comparative Analysis ◽  
Aromatic Hydrocarbons ◽  
Limited Information ◽  
Acid Transport ◽  
Single Strain ◽  
Degrading Bacterium ◽  
Genes Encoding ◽  
Polycyclic Aromatic

The genomes ofAdvenellahave been sequenced to date, which are important bacteria in environment. However, the genome of a single strain can only provide limited information on genetic diversity. Here, genomes ofAdvenellawere compared by bioinformatics methods. A total of 2438 genes were shared by 3 strains ofAdvenella. Amino acid transport and metabolism related genes dominated the shared genes ofAdvenella, indicates the importance of these genes to the survival ofAdvenella. Genes encoding protocatechuate 3,4-dioxygenase was highly conserved in the genusAdvenella. This comparative analysis presents a foundation for future understanding of the biodiversity ofAdvenella.

Virulence and Molecular Diversity in the Kabatiella zeae Population Causing Maize Eyespot in China

Plant Disease ◽  
2020 ◽  
Vol 104 (12) ◽  
pp. 3197-3206
Author(s):  
Nan Chen ◽  
Shuqin Xiao ◽  
Jiaying Sun ◽  
Lu He ◽  
Miaomiao Liu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Diversity ◽  
Fragment Length Polymorphism ◽  
Genotypic Diversity ◽  
Limiting Factor ◽  
Aflp Analysis ◽  
Limited Information ◽  
Pathogen Population ◽  
Nomenclature System ◽  
Differential Hosts

Maize eyespot, caused by Kabatiella zeae, has become a major yield-limiting factor in maize planting areas in northeast China. Limited information is available on pathotypes, virulence, and the genetic diversity of the K. zeae population. We analyzed virulence and genetic diversity of 103 K. zeae isolates collected from six provinces in China with differential hosts and the amplified fragment length polymorphism (AFLP) technique, respectively. To evaluate the virulence, 103 isolates were inoculated on nine differential hosts (maize inbred lines)—E28, Shen137, Qi319, B73, Danhuang34, Zi330, Mo17, Huangzaosi, and CN165—and grouped into 23 pathotypes and three virulence groups according to the coded triplet nomenclature system on differential hosts. AFLP analysis resolved the set of isolates into four genetic diversity clusters (DICE similarity values of 76%). Genetic variation of K. zeae among and between pathotypes revealed that the pathogen population had a high genotypic diversity. The correlation between pathotypes, virulence, and genetic diversity grouping was low. A correlation between AFLP groups and geographic locations was detected.

Blood protein polymorphism and genetic diversity in locally adapted Muscovy duck (Cairina moschata) in Nigeria

2015 ◽  
Vol 56 ◽  
pp. 9-18
Author(s):  
A.O. Oguntunji ◽  
K.L. Ayorinde
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Rain Forest ◽  
Enzyme Polymorphism ◽  
Blood Protein ◽  
Muscovy Duck ◽  
Blood Proteins ◽  
Evolutionary Forces ◽  
Guinea Savanna ◽  
Derived Savanna

SummaryCharacterization and genetic diversities among members of a species are fundamental to their improvement and conservation. This study was conducted to characterize and estimate genetic diversity in the ecotypes of the locally adapted Muscovy duck in Nigeria using blood proteins (haemoglobin, transferrin and albumin) and enzyme (carbonic anhydrase) markers. Blood samples collected from 20, 40 and 20 adult Muscovy ducks sampled randomly from the Rain Forest, Derived Savanna and Guinea Savanna ecotypes, respectively, were typed for blood proteins and enzyme polymorphism with cellulose acetate paper. Genetic variability in the studied population was accessed using heterozygosity (H), effective number of allele (ne) and polymorphism (percent P). All the 13 allelic variants expressed at the four loci were expressed in the Derived Savanna ecotype but 12 each in the Rain Forest and Guinea Savanna ecotypes. Results showed that all the four loci were polymorphic (100 percent) and the estimated heterozygosity among them was similar (0.424–0.481). Similarity in the estimated genetic variability parameters among ecotypes indicate that the sub-populations understudy was under similar evolutionary forces and there were no appreciable differences among them.

scholarly journals Shelter cats host infections with multiple Trypanosoma cruzi discrete typing units in southern Louisiana

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Eric Dumonteil ◽  
Hans Desale ◽  
Weihong Tu ◽  
Brandy Duhon ◽  
Wendy Wolfson ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Trypanosoma Cruzi ◽  
Deep Sequencing ◽  
Limited Information ◽  
Convenience Sample ◽  
Seropositivity Rate ◽  
Southern Us ◽  
Zoonotic Parasite ◽  
Local Transmission ◽  
Southern Louisiana

AbstractTrypanosoma cruzi is a zoonotic parasite endemic in the southern US and the Americas, which may frequently infect dogs, but limited information is available about infections in cats. We surveyed a convenience sample of 284 shelter cats from Southern Louisiana to evaluate T. cruzi infection using serological and PCR tests. Parasites from PCR positive cats were also genotyped by PCR and deep sequencing to assess their genetic diversity. We detected a seropositivity rate for T. cruzi of at least 7.3% (17/234), and 24.6% of cats (70/284) were PCR positive for the parasite. Seropositivity increased with cat age (R2 = 0.91, P = 0.011), corresponding to an incidence of 7.2% ± 1.3 per year, while PCR positivity decreased with age (R2 = 0.93, P = 0.007). Cats were predominantly infected with parasites from TcI and TcVI DTUs, and to a lesser extent from TcIV and TcV DTUs, in agreement with the circulation of these parasite DTUs in local transmission cycles. These results indicate that veterinarians should have a greater awareness of T. cruzi infection in pets and that it would be important to better evaluate the risk for spillover infections in humans.

scholarly journals Morphological and Genetic Diversity Studies of Chrysichthys nigrodigitatus from the Cross River, Nigeria, Using Microsatellite Markers

2020 ◽  
pp. 42-52
Author(s):  
Chioma Adelieje ◽  
Edak Aniedi Uyoh ◽  
Valentine Otang Ntui ◽  
Daniel Ama-Abasi
Keyword(s):  
Genetic Diversity ◽  
Principal Component ◽  
Conservation Strategies ◽  
Head Width ◽  
Limited Information ◽  
Term Survival ◽  
Cross River ◽  
Fish Samples ◽  
Diversity Studies ◽  
Two Populations

Morphometric and genetic diversity studies were carried out on Chrysichthys nigrodigitatus sampled from middle and lower Cross River. The aim was to provide information on variations between these two populations and also augment the limited information currently available on genetic diversity in this species. A total of 79 fish samples were used for the morphometric analysis out of which 30 were used for the genetic study. Genomic DNA was extracted from caudal fin using a modified cetyltrimethylammonium bromide method and amplified using microsatellite markers.Twenty-three morphological features were studied from each fish sample out of which 19 showed significant differences (P<0.05) between the two populations. Principal Component Analysis identified head length, head width, caudal peduncle depth, standard length, preventral distance, snout length and anal fin length as key contributors to variation. Genetic analyses indicated low variability in the populations studied as evidenced by low Shannon`s information index (mean of 0.944 – 1.034), and positive coefficients of inbreeding (FIS) across both populations suggesting the presence of greater homozygosity in this species. Gene flow of 3.507 was observed between the lower and middle Cross River indicating the possibility of free mating between the two populations. The low levels of genetic diversity call for urgent management and conservation strategies to ensure long term survival of the species.

scholarly journals The Effects of Forest Management Activities on Genetic Diversity of Forest Trees

2021 ◽  
Vol 2 (2) ◽  
pp. 110-118
Author(s):  
Subash Gautam ◽  
Sachin Timilsina ◽  
Manish Shrestha
Keyword(s):  
Genetic Diversity ◽  
Forest Management ◽  
Management Practices ◽  
Environmental Changes ◽  
Stand Structure ◽  
Forest Products ◽  
Forest Trees ◽  
Limited Information ◽  
Forest Management Practices ◽  
Research Activities

Genetic diversity helps to survive forest trees in several environmental changes and disease conditions. Different forest management activities such as harvesting, thinning, natural or artificial regeneration, seedlings or coppice forests, fragmentation, and overexploitation have a tremendous influence on the genetic diversity and population structure of forest trees. This paper aimed to review the impacts of these activities on the genetic diversity of forest trees. For this, we reviewed several scientific literature related to forest management practices that affect genetic diversity. Altogether,75 papers were reviewed, interpreted, and evaluated to prepare our final manuscript. The result of this study recommends that the level of genetic impacts varies with management activities, stand structure as well as species characteristics. There is very limited information about the impacts of forest management practices on the genetic diversity of forest trees since it is only focused on the growth of stands. The field research activities for species-specific must be executed considering ecological and reproductive parameters to assure sustainable forest ecosystems. Hence, this review will be beneficial for forest conservationists, researchers, and managers for the management of forests through better forest management activities preserving a genetic pool of the forest trees, and sustainable utilization of forest products.  

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