Keragaman genotipik entok (Cairina moschata) berdasarkan polimorfisme protein darah

Objective: The aim of this study was to identified genetic diversity of muscovy duck in Central Java through blood protein polymorphisms by using electrophoresis method.Methods: Blood sample was collected from a total of 60 muscovy ducks from the districts of Demak, Magelang and Pekalongan, 20 samples each. The Electrophoresis Polyacrylamide Gel of Electrophoresis–Thin Layer Electrophoresis (PAGE-TLE) was used in this study. Parameters observed were protein of albumin (Alb), ceruloplasmin (Cp), transferrin (Tf) and amylase-I (Am-I). Gene frequency, heterozygosity (Ho) and genetic distance were analyzed by using DISPAN program.Results: The results showed that the Alb, Cp, Tf and Am-I of muscovy duck showing polymorphic characters. Gene frequency of AlbB (0.61) was higher compared to AlbA (0.39). Gene frequency of CpA (0.62) was higher than the CpB (0.38). Furthermore, gene frequency of TfA and TfB were 0.32 and 0.68, respectively as well as gene frequency of Am-IA (0.82) was higher than Am-IB (0.18). Muscovy duck population of Demak showed higher Ho value for Alb and Am-I, whereas Magelang showed higher value for Cp and Tf. Population of muscovy duck of Demak showed closer genetic distance to Pekalongan than Magelang.Conclusions: The protein blood locus of three population of muscovy duck in Central Jawa showed polymorphic characters.

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Polymorphisme Gene GH and Morphological Characteristic of Anas platyrhynchos and Cairina moschata

ANIMAL PRODUCTION ◽

10.20884/1.jap.2018.20.1.665 ◽

2019 ◽

Vol 20 (1) ◽

pp. 17

Author(s):

Firman Febrianto ◽

Ismoyowati Ismoyowati ◽

Mochamad Mufti ◽

Prayitno Prayitno ◽

Dattadewi Purwantini

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Anas Platyrhynchos ◽

Quantitative Difference ◽

Muscovy Duck ◽

Qualitative Properties ◽

Black And White ◽

Cairina Moschata ◽

Genotype Frequencies ◽

Gh Gene

The aims of this research was to identify the qualitative and quantitative difference of the phenotypes of native mallard and Muscovy, and Muscovy the genetic diversity between Muscovy and mallard using RFLP method with growth hormone primer (GH) gene. The materials were 30-week old male and female ducks from 5 strains—Magelang, Mojosari and Tegal ducks, white-feathered, and black and white-feathered Muscovy. Muscovy Completely Randomized Design was used to analyze parameters 5 strains of ducks based on gender and with 5 replications. The parameters measured were local and Muscovy duck phenotypes and genetic diversity (number of alleles) based on GH gene, heterozygosity and genetic distance. Qualitative properties were analyzed descriptively and quantitative properties were analyzed using Anova followed by HSD in case of significant differences, whereas RFLP analysis was used to determine the allele frequencies, genotype frequencies, genetic diversity, and genetic distance of local ducks. Result showed phenotypic differences between Anas platyrhynchos and Cairina moschata. The dominant feather colorof Muscovy was black and white while mallard was brown. The bill color Muscovy Muscovy was pink with dark brown but black in mallard. The dominant shank’s color in both Muscovy and mallard was black. Body size of mallard was larger than that of Muscovy Muscovy, and Magelang ducks weigherd more than Tegal and Mojosari Duck. The PCR-RFLP results showed lower heterozygosity of mallard compared to Muscovy based on GH gene, and genetic distance of the Tegal duck was closer to Mojosari and Magelang ducks, whereas Magelang and Mojosari ducks had a considerable genetic distance based on the GH gene.

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Keragaman Genetik Kukang Jawa (Nycticebus javanicus) Menggunakan Control Region (D-loop) DNA Mitokondria (mtDNA) (GENETIC DIVERSITY ON JAVAN SLOW LORIS (NYCTICEBUS JAVANICUS) USING OF CONTROL REGION (D-LOOP) mtDNA)

jurnal veteriner ◽

10.19087/jveteriner.2019.20.3.360 ◽

2019 ◽

Vol 20 (3) ◽

pp. 360

Author(s):

Wirdateti Wirdateti ◽

Hayati Aziza ◽

Handayani Handayani

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Control Region ◽

West Java ◽

Slow Loris ◽

Genetic Population ◽

Central Java ◽

Low Genetic Diversity ◽

The Status ◽

D Loop

Javan slow loris (Nycticebus javanicus) one of the species of the genus Nycticebus is endemic in Java. Their distribution region is in West Java and Banten, and also reported to be found in Central Java and East Java, although very rarely. Status of the species is Critically Endangered due to high levels of poaching, habitat loss and habitat fragmentation, so that the necessary conservation in order to increase the population. For conservation management it is necessary to know the status of genetic resources that play of role in breeding, then this research is to explore the genetic population of the Javan slow loris from some locations in West Java. The research objective was to assess the diversity of their current population of Java loris through the control region (D-loop) of mitochondrial DNA (mtDNA). A total of 23 individuals samples from Gunung Halimun Park, Tasikmalaya, Garut, Ciamis, Jember and confiscated at the Rehabilitation Center IAR Bogor were used in this stydy. Specific primers of D-loop are used for loris with a length of 296 bp sequence. The result showed there are only five different sites and formed six haplotypes, each haplotypes only 1-3 nucleotides different. Low genetic diversity is shown as much as 42.96% of individuals show the same sequence or genetic distance (d) = 0 that indicate was monomorf population from different population. The genetic distance of the entire population was 0.003 ± 0:01 (0.3%).

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First report of Anisakis sp. (Nematoda: Anisakidae) parasitizing Muscovy duck in Marajó Island, state of Pará, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612020015 ◽

2020 ◽

Vol 29 (2) ◽

Author(s):

Elaine Lopes de Carvalho ◽

Ricardo Luis Sousa Santana ◽

Evonnildo Costa Gonçalves ◽

Raul Henrique da Silva Pinheiro ◽

Elane Guerreiro Giese

Keyword(s):

Climatic Conditions ◽

Human Consumption ◽

Esophageal Mucosa ◽

Muscovy Duck ◽

Cairina Moschata ◽

Island State ◽

Third Stage ◽

Marajó Island ◽

Northern Brazil ◽

Muscovy Ducks

Abstract Among the avian used for human consumption, the Muscovy duck is well adapted to various climatic conditions and its breeding is widespread due to its easy handling, and its meat is widely consumed and appreciated, especially in the cuisine of northern Brazil. The present study aimed to report and identify taxonomically the nematodes found in the esophagus of Muscovy ducks reared and marketed in the municipality of Soure, Marajó Island, state of Pará, Brazil, and discuss its zoonotic potential to human. The samples consisted of 30 specimens of Cairina moschata domestica analyzed. A total of 258 nematodes were recovered, which were strongly fixed in the esophageal mucosa of hosts. The morphological and morphometric characteristics were compatible with Anisakis third-stage larvae.

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Blood protein polymorphism and genetic diversity in locally adapted Muscovy duck (Cairina moschata) in Nigeria

Animal Genetic Resources/Ressources génétiques animales/Recursos genéticos animales ◽

10.1017/s2078633614000526 ◽

2015 ◽

Vol 56 ◽

pp. 9-18

Author(s):

A.O. Oguntunji ◽

K.L. Ayorinde

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Rain Forest ◽

Enzyme Polymorphism ◽

Blood Protein ◽

Muscovy Duck ◽

Blood Proteins ◽

Evolutionary Forces ◽

Guinea Savanna ◽

Derived Savanna

SummaryCharacterization and genetic diversities among members of a species are fundamental to their improvement and conservation. This study was conducted to characterize and estimate genetic diversity in the ecotypes of the locally adapted Muscovy duck in Nigeria using blood proteins (haemoglobin, transferrin and albumin) and enzyme (carbonic anhydrase) markers. Blood samples collected from 20, 40 and 20 adult Muscovy ducks sampled randomly from the Rain Forest, Derived Savanna and Guinea Savanna ecotypes, respectively, were typed for blood proteins and enzyme polymorphism with cellulose acetate paper. Genetic variability in the studied population was accessed using heterozygosity (H), effective number of allele (ne) and polymorphism (percent P). All the 13 allelic variants expressed at the four loci were expressed in the Derived Savanna ecotype but 12 each in the Rain Forest and Guinea Savanna ecotypes. Results showed that all the four loci were polymorphic (100 percent) and the estimated heterozygosity among them was similar (0.424–0.481). Similarity in the estimated genetic variability parameters among ecotypes indicate that the sub-populations understudy was under similar evolutionary forces and there were no appreciable differences among them.

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Hubungan Genetik antara Domba Wonosobo (Dombos), Domba Ekor Tipis (DET) dan Domba Batur (Dombat) Melalui Analisis Polimorfisme Protein Darah

Sains Peternakan ◽

10.20961/sainspet.v11i1.4798 ◽

2017 ◽

Vol 12 (1) ◽

pp. 1

Author(s):

F Noviani ◽

Sutopo Sutopo ◽

E Kurnianto

Keyword(s):

Thin Layer ◽

Genetic Distance ◽

Blood Plasma ◽

Key Words ◽

Genetic Relationship ◽

Gel Electrophoresis ◽

Gene Frequency ◽

Genetic Relationships ◽

Blood Protein ◽

Polymorphism Analysis

The objective of this study was to evaluate the genetic relationships among Wonosobo sheep (Dombos), Thin-Tailed sheep (DET) and Batur sheep (Dombat) through blood protein polymorphism analysis by using electrophoresis Polyacrylamid Gel Electrophoresis-Thin Layer Electrophoresis (PAGE-TLE). The blood of the 20 head of Dombos (Wonosobo regency), 20 head of DET (Semarang regency) and 20 head of Dombat (Banjarnegara regency) were used as materials. Six locus of blood plasma protein were observed, then the gene frequency, individual heterosigosity, average heterosigosity and genetic distance among three breed were calculated. The results showed that the locus of pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf) and amylase-I (Am-I), at DET, Dombos and Dombat were polymorphic (0,380-0,454). In conclusion, the DET and Dombat indicated the closer genetic relationship compared to DET-Dombos and Dombos-Dombat. Key words: sheep, polymorphism, genetic relationship

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Performa Pertumbuhan dan Produksi Karkas Mandalung Hasil Hibridisasi antara Entok Jantan (Cairina moschata) dengan Itik Betina (Anas plathyrynchos) (The Growth Performance and Production of Carcass Mandalung, Hybridized from Male Muscovy Ducks (Cairina moschata) and Female Ducks (Anas plathyrynchos))

Jurnal Sains Terapan ◽

10.29244/jstsv.5.1.8-16 ◽

2015 ◽

Vol 5 (1) ◽

pp. 8-16

Author(s):

Danang Priyambodo ◽

Jakaria Jakaria ◽

Rukmiasih Rukmiasih

Keyword(s):

Growth Performance ◽

Block Design ◽

Muscovy Duck ◽

Average Value ◽

Cairina Moschata ◽

Randomized Block Design ◽

Heterosis Effect ◽

Muscovy Ducks ◽

Mule Duck ◽

Species Hybridization

Crossbreeding between species (hybridization) was conducted to obtain higher average value of offspring trait than the parent trait. The aim of this study was to determine the best type of mule duck and to evaluate the heterosis effect appearing on mule duck obtained. The experimental design used was a randomized block design with two levels of treatment and eight groups based on the hatching period. The treatments were the type of mule duck crossed between muscovy duck male with female cihateup duck (EC) and mule duck crossed between male muscovy duck with female alabio duck (EA). The results showed the growth performance and carcass production of EC and EA mule duck were not significantly different, but mule duck EC tended to show better results than mule duck EA. The percentage of heterosis effect produced by EC and EA mule duck in growth performance and carcass production showed advantages over the parent. EC mule duck had a higher level of heterosis than EA mule duck for some trait observed. EC mule duck can be kept as best crossbreed to produce mule duck with good performance and carcass production.Keywords: mule duck, performance, carcass production, heterosis

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Polimorfisme Protein Darah Itik Pegagan dengan Metode PAGE

Jurnal Agripet ◽

10.17969/agripet.v11i2.376 ◽

2011 ◽

Vol 11 (2) ◽

pp. 58-62

Author(s):

Meisji Liana Sari ◽

Ronny Rachman Noor ◽

Peni S. Hardjosworo ◽

Chairun Nisa

Keyword(s):

Genetic Diversity ◽

Blood Protein ◽

Limited Information ◽

Genetic Characteristic ◽

Duck Species ◽

Protein Locus ◽

Electrophoresis Method

polymorphism of blood protein pegagan duck by PAGE methodABSTRACT. Pegagan duck is one of local duck species which comes from Ogan Ilir (OI) Regency, South Sumatera. This species is not popular, yet. But, it has spread out along Ogan river which comprises of three districts e.g Tanjung Raja, Inderalaya and Pemulutan. The ecosystem in this three districts is dominated by swampy area. As far, there is relatively limited information of Pegagan duck compared to another local duck. So, for the first step was done a reseach to identify genetic characteristic from blood protein analyses by electrophoresis method. It is expected that this information will become the bases in determining of the policy of Pegagan duck development. Polymorphism analyses of blood protein was done with 9 duck samples which taken randomly from 400 pegagan ducks. Protein locus observed were albumin (Alb), post albumin (Pa), transferrine (Tf), post transferrine-1 (Ptf-1), and post transferrine-2 (Ptf-2). Polymorphism analyses of blood protein of pegagan duck was characterized by allele A in locus Transferrine (TfA), Post transferrine-1 (Ptf-1A) and Post transferrine-2 (Ptf-2A) . Genetic diversity based on blood protein of Pegagan duck showed that gen frequency value in a range of 0,11 – 0,94 and high heterozygosis value that was 0,62 %.

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Limited Genetic Diversity of Chinese Muscovy Duck (Cairina moschata) Revealed by Partial Sequences of Mitochondrial DNA Cytochrome b Gene

Advances in Intelligent and Soft Computing - Information Technology and Agricultural Engineering ◽

10.1007/978-3-642-27537-1_35 ◽

2012 ◽

pp. 279-282 ◽

Cited By ~ 1

Author(s):

Jing Sun ◽

Jing Huang ◽

Xiaoling Zhao ◽

Hang Zhong ◽

Qing Zhu ◽

...

Keyword(s):

Genetic Diversity ◽

Mitochondrial Dna ◽

Cytochrome B ◽

Cytochrome B Gene ◽

Muscovy Duck ◽

Cairina Moschata ◽

Limited Genetic Diversity

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Short Communication: Genetic diversity of Ongole Grade Cattle of Rembang District, Central Java, Indonesia, based on blood protein polymorphism

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d190432 ◽

2018 ◽

Vol 19 (4) ◽

pp. 1429-1433

Author(s):

SUTIYONO SUTIYONO ◽

SUTOPO SUTOPO ◽

Y. S. ONDHO ◽

SETIATIN SETIATIN ◽

D. SAMSUDEWA ◽

...

Keyword(s):

Genetic Diversity ◽

Short Communication ◽

Sodium Dodecyl ◽

Protein Polymorphism ◽

Blood Protein ◽

Blood Proteins ◽

Blood Samples ◽

Central Java ◽

Sds Page ◽

Almost All

Sutiyono, Sutopo, Ondho YS, Setiatin ET, Samsudewa D, Suryawijaya A, Lestari DA, Kurnianto E. 2018. Genetic diversity ofOngole Grade Cattle of Rembang District, Central Java, Indonesia, based on blood protein polymorphism. Biodiversitas 19: 1429-1433. The objective of this study was to identify genetic diversity in Ongole Grade cattle of Rembang District, Central Java, Indonesia,based on blood-protein polymorphism. A total of 34 blood samples were collected from unrelated Ongole Grade cattle and the bloodplasm was used to identify the blood proteins. Blood protein analysis was performed using Sodium Dodecyl Sulfate-Polyacrilamide GelElectrophoresis (SDS-PAGE). Observed bands were used to perform allele interpretation of four loci, namely Albumin (Alb), Postalbumin(Po-Alb), Ceruloplasmin (Cp) and Amylase-1 (Am-1). Results showed the presence of polymorphism in all of the four bloodproteinloci, average heterozigosity value (H) being 0.506 and almost all of the blood-protein loci studied showed deviation from Hardy-Weimberg Equilibrium (HWE). In conclusion, the study indicated that population of Ongole Grade cattle in Rembang, Indonesia hashigh genetic diversity.

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