scholarly journals Uji masa simpan pelet Trichoderma harzianum dan kemampuannya dalam menghambat perkembangan penyakit Layu Fusarium pada bibit tomat.

2018 ◽  
Vol 3 (2) ◽  
pp. 117-127
Author(s):  
Rizka Musfirah ◽  
Rina Sriwati ◽  
Tjut Chamzurni
Keyword(s):  
Vitamin A ◽  
Shelf Life ◽  
Fusarium Oxysporum ◽  
Solanum Lycopersicum ◽  
Plant Height ◽  
Incubation Period ◽  
Fusarium Wilt ◽  
Trichoderma Harzianum ◽  
Wilt Disease ◽  
Damping Off

Abstrak. Tomat (Solanum lycopersicum) merupakan salah satu komoditas pertanian yang ditanam secara luas di seluruh dunia, termasuk di Indonesia, karena memiliki rasa yang khas dan enak, juga memiliki nilai gizi seperti sumber vitamin A dan C yang sangat baik. Produksi tomat mengalami penurunan setiap tahun, salah satunya diakibatkan oleh organisme penganggu tanaman (OPT) yaitu patogen Fusarium oxysporum sehingga perlu dilakukan pengendalian hayati yaitu menggunakan Trichoderma harzianum dalam bentuk formulasi pelet yang praktis, efektif, dan efesien. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 6 perlakuan dengan 3 ulangan, setiap perlakuan terdiri dari 10 unit bibit tomat. Penelitian ini terdiri dari 6 perlakuan yaitu perlakuan A (masa simpan pelet T. harzianum  4 minggu), B (masa simpan pelet T. harzianum 3 minggu), C (masa simpan pelet T. harzianum 2 minggu), D (masa simpan pelet T. harzianum 1 minggu), E (masa simpan pelet T. harzianum 0 minggu), F (tanpa perlakuan pelet T. harzianum). Peubah yang diamati yaitu pre-emergence damping off, post-emergence damping off, masa inkubasi, persentase tanaman layu, tinggi tanaman, dan jumlah daun. Hasil penelitian menunjukkan bahwa pelet T. harzianum yang disimpan 4 minggu efektif dalam menghambat perkembangan penyakit layu fusarium seperti menunda masa inkubasi sampai 7 HSI, menekan pre-emergence damping off sampai 90%, post-emergence damping off 92,95%, serta mampu meningkatkan tinggi tanaman sampai 19,63 cm dan meningkatkan jumlah daun rata-rata 7 helai pada 35 HSI. (Storing Period of Trichoderma harzianum Pellets and its ability to Inhibit the development of Fusarium Wilt Disease on Tomato Seeds)Abstract. Tomato (Solanum lycopersicum) is one of the most widely grown commodities in the world, including Indonesia. It has a distinctively good taste and many nutritional value such as vitamin A and C. However, tomato production has decreased every year. One of the main cause is the attacks by pathogens, named Fusarium oxysporum. A Biological control is necessary and the use of Trichoderma harzianum in the form of pellets is recommended because of its effectiveness, efficiency and practical use. This research used a Completely Randomized Design (RAL) non-factorial consisted of 6 treatments with 3 replications, each treatment consisted of 10 units of tomato seedlings. The 6 treatments are named as treatment A (T. harzianum pellet saving 4 weeks), B (T. harzianum pellet saving period 3 weeks), C (shelf life of 2 weeks T. harzianum pellet), D (shelf life of pellet T harzianum 1 week), E (shelf life of pellet T. harzianum 0 weeks), and F (without T. harzianum pellet treatment). The variables observed in this study are pre-emergence damping off, post-emergence damping off, incubation period, the percentage of wilted plants, plant height, and the number of leaves. The results showed that pellets of T. harzianum stored 4 weeks effectively inhibiting the development of fusarium wilt disease such as delaying incubation period up to 7 HSI (Days After Incubation), suppressing the pre-emergence damping off up to 90% and post-emergence damping off to 92.95%, also able to increase the plant height up to 19.63 cm and increase the average leaf number of 7 strands at 35 HSI.

scholarly journals Effect of Biological Fertilizer and Chemical Fungicide on Fusarium Wilt Disease, Growth and Yield on Onion (Allium ascaloncum L)

Agrologia ◽  
2021 ◽  
Vol 10 (2) ◽  
Author(s):  
Rafit Syarifudin ◽  
A.Marthin Kalay ◽  
Costanza Uruilal
Keyword(s):  
Fusarium Oxysporum ◽  
Plant Height ◽  
Fusarium Wilt ◽  
Block Design ◽  
Economic Value ◽  
Wilt Disease ◽  
Growth And Yield ◽  
Fusarium Wilt Disease ◽  
Randomized Block Design ◽  
Biological Fertilizer

Onion (Allium Ascalonicum L) are one of the leading horticultural commodities that have economic value. In cultivation, Fusarium wilt disease is often damaged caused by the fungus Fusarium oxysporum. This study aimed to examine the effect of biofertilizer application on fusarium wilt disease, growth and yield of onion. The treatments tested were Bion-up biofertilizer, KIBRT, Biostimulant, Azoto-Tricho, anthracol fungicide, and without biological fertilizer as a control. Using a randomized block design with three replications. Observation variables were the incidence of fusarium wilt disease, plant height, number and weight of tubers. The results showed that the application of biofertilizers (Bion up, KIBRT, Biostimulants and Azoto-Tricho) was effective in increasing plant height, number of bulbs, and bulb weight of onion bulbs, while controlling the development of fusarium wilt disease was more effective when using Bion up, KIBRT, and Azoto-Tricho.Keywords: Biofertilizer, Antracol, Shallots, Fusarium oxysporum

scholarly journals Combination of Trichoderma viride and Pseudomonas fluorescens for the enhanced control of Fusarium wilt disease caused by Fusarium oxysporum infecting Arachis hypogaea L.

2019 ◽  
Vol 11 (1) ◽  
pp. 138-143 ◽  
Author(s):  
P. Rajeswari
Keyword(s):  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Arachis Hypogaea ◽  
Fusarium Wilt ◽  
Trichoderma Harzianum ◽  
Trichoderma Viride ◽  
Amino Nitrogen ◽  
Wilt Disease ◽  
Fusarium Wilt Disease ◽  
Viable Approach

Fusarium wilt caused by Fusarium oxysporum is a devastating disease of peanut. The fungus causes severe yield loss in groundnut. Combinations of biocontrol agents that are compatible with each other is a viable approach to control the plant disease. The study was conducted to determine the beneficiary aspects of  combining different species of Trichoderma and Pseudomonasfluorescens i.e Trichoderma viride+Pseudomonas fluorescens (Tv+Pf), Trichoderma harzianum+Pseudomonas fluorescens (Th+Pf) and Trichoderma viride +Trichoderma harzianum (Tv+Th) to control the Fusarium wilt  in  biochemicalparameters such as DNA, RNA, Amino nitrogen, phenols, dihydroxy and   proline  contents of Arachis hypogaea.L. Among the three combinations tested, Trichoderma viride + Pseudomonas fluorescens (1+2%) sprayed leaves provided greater suppression of Fusariumoxysporum by increasing the levels of DNA,RNA,Amino nitrogen contents resulting  in the suppression of  Fusarium wilt  disease of Arachis hypogaea L.Maximum  reduction of  DNA, RNA, Amino nitrogen was observed in the infected Fusariumoxysporumleaves Phenol, Dihydroxy phenols and proline contents increase sharply in the treated plants treated with (Tv+Pf) as compared to the control plants. At the same time the other two combinations resulted in  enhanced control  in  comparison with  individual ones. This present study indicates that specific combination of Trichoderma viride and Pseudomonas fluorescens could have the greater efficacy in the inhibition of pathogen  in the biocontrol of Fusarium wilt  disease  as   compared   with   individual strains.

scholarly journals EVALUASI KETAHANAN AKSESI KENAF (Hibiscus cannabinus L.) TERHADAP PENYAKIT LAYU Fusarium oxysporum SCHLECT

2020 ◽  
Vol 6 (2) ◽  
pp. 29
Author(s):  
GEMBONG DALMADIYO ◽  
CECE SUHARA ◽  
SUPRIYONO SUPRIYONO ◽  
SUDJINDRO SUDJINDRO
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Hibiscus Cannabinus ◽  
Resistant Variety ◽  
Fusarium Wilt Disease ◽  
Screen House

<p><strong>Evaluation on the resistance of kenaf accessions (Hibis¬ cus cannabinus /..) to Fusarium oxysporum Schlect</strong></p><p>Resistant variety is one of the most important components controlling of fusarium wilt disease on kenaf caused by Fusarium oxysporum Schlect. To ind out resistant variety an evaluation on kenaf accessions was conducted in the laboratory and screen house of Phytopathology, RITFC, Malang in June-December 1997. The results of the selection on 77 accessions showed that 41 accessions were highly resistant, 1 2 accessions were resistant, 7 accessions were moderate, 12 accessions were susceptible, and 5 accessions were highly susceptible Three resistant and highly resistant accessions were namely 85-9-73, DS/005 H, and FJ/004 He could inhibit F. oxysporum growth about 23.40- 32.43 mm and its discolorisation about 0.0-13.4%.</p>

scholarly journals Seleksi Bakteri Antagonis untuk Mengendalikan Layu Fusarium pada Tanaman Phalaenopsis

2013 ◽  
Vol 22 (3) ◽  
pp. 276 ◽  
Author(s):  
Ika Djatnika
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Antagonistic Bacteria ◽  
Plant Research Institute ◽  
Fusarium Spp ◽  
Human Environment ◽  
Bacterial Antagonists ◽  
Important Disease ◽  
Selection Of

<p>ABSTRAK. Layu Fusarium merupakan penyakit penting yang menjadi kendala dalam memproduksi tanaman anggrek. Untuk mengendalikannya,  petani masih menggunakan fungsida. Tanaman anggrek kerap ditampilkan sebagai hiasan yang dekat dengan lingkungan manusia, maka penggunaan pestisida perlu diperhatikan. Oleh karena itu sangat penting dicari cara pengendalian lainnya yang aman terhadap lingkungan, antara lain dengan  pengendalian hayati. Tujuan penelitian ialah mendapatkan isolat bakteri antagonis yang dapat mengendalikan layu Fusarium pada tanaman Phalaenopsis. Percobaan dilakukan di Laboratorium dan Rumah Kasa Balai Penelitian Tanaman Hias Segunung (1100 m dpl.) serta untuk  mikrob diisolasi dari lokasi tanaman hortikultura di Jawa Barat dan DKI Jakarta, mulai Bulan Januari sampai dengan Desember 2010. Penelitian meliputi isolasi Fusarium spp. sebagai patogen pada tanaman anggrek di beberapa lokasi, isolasi bakteri antagonis, uji kemangkusan bakteri terhadap pertumbuhan Fusarium spp. di laboratorium, dan uji kemangkusan bakteri antagonis terhadap layu Fusarium di rumah kasa. Hasil penelitian menunjukkan bahwa penyebab layu Fusarium pada tanaman Phalaenopsis ialah Fusarium oxysporum. Dari 154 isolat bakteri yang diisolasi dari lapangan, hanya ada tiga  isolat yaitu nomor B23, B 26, dan B37 yang dapat menekan pertumbuhan F. oxysporum pada media PDA. Sampai dengan pengamatan minggu ke-10 setelah inokulasi, ketiga bakteri tersebut masing-masing menekan jumlah tanaman yang terserang layu Fusarium, yaitu sebesar 46,9; 48,9; dan 65,3%, dan masing-masing menekan intensitas penyakit layu 50,5; 43,9; dan 55,1%.</p><p>ABSTRACT. Djatnika, I 2012. Selection of Antagonistic Bacteria for Controlling of Fusarium Wilt on Phalaenopsis Plants. Fusarium wilt is an important disease as constraint on production of orchid plants. The control of Fusarium wilt of orchids with fungicides often use by farmers. Orchid plants are often displayed as a decoration which is close to the human environment, so the application of pesticides have to get attention. It is therefore necessary to find another method that is safe for environments, such as using of biological control. The purpose of the study was to get isolates of  bacterial antagonists for controlling of Fusarium wilt of Phalaenopsis plants. The experiment was conducted at Laboratory and Screenhouse of Indonesian Ornamental Plant Research Institute, Segunung (1100 m asl.) and the microbes were isolated from horticultural area in West Java and DKI Jakarta since January until December 2010. The research comprised of isolation of Fusarium spp. from orchid plants in some location, isolation of bacterial antagonists, the effectiveness of the bacteria to suppress Fusarium spp. growth in laboratory, and the effectiveness of the bacteria to control Fusarium wilt on Phalaenopsis plants in the screenhouse. The results showed that the causal Fusarium wilt of Phalaenopsis plants was identified as Fusarium oxysporum. Three of 154 isolates of bacteria, i.e. isolates number of B23, B26, and B37, could suppress of F. oxysporum growth on PDA media. Observation up to 10 weeks after inoculation, the three bacteria could reduce the number of  plants attacked by Fusarium wilt , which were 46.9; 48.9; and  65.3% respectively, and each of them suppress wilt disease intensity 50.5, 43.9, and 55.1% respectively. <br /><br /></p>

scholarly journals Kombinasi Beberapa Dosis dan Spesies Trichoderma Formulasi Pelet dalam Menekan Perkembangan Jamur Fusarium oxyporum f.sp capsici di Pembibitan Cabai Merah ( Capsicum annum L.)

2020 ◽  
Vol 3 (2) ◽  
pp. 70-79
Author(s):  
Frara Efeseli Fakhdian ◽  
Abduh Ulim ◽  
Tjut Chamzurni
Keyword(s):  
Fusarium Oxysporum ◽  
Incubation Period ◽  
Trichoderma Harzianum ◽  
Plant Protection ◽  
High Price ◽  
Selling Price ◽  
Trichoderma Virens ◽  
Study Program ◽  
Capsicum Annum ◽  
Pepper Plants

Abstrak:Cabai merah (Capsicum annum L.) merupakan salah satu komoditas hortikultura unggulan terbesar dari golongan sayur-sayuran karena memiliki harga jual yang tinggi. Disamping itu selain harga yang tinggi, di lapangan cabai merah sering  mengalami gangguan dari mulai benih disemai sampai tanaman cabai menghasilkan, hal ini disebabkan penyakit tular tanah atau pun terbawa benih. Salah satu penyakit yang memprihatinkan pada tanaman cabai merah adalah penyakit layu yang disebabkan oleh jamur Fusarium  oxysporum f.sp capsici,penyakit ini sering merugikan para petani, jika tanaman sudah terserang oleh penyakitini,  kerugian bisa mencapai 80 % pada tanaman cabai merah. Salah satu alternatif  yang digunakan dalam Pengendalian Hama dan Penyakit terpadu (PHPT) dengan menggunakan agensia Trichoderma formulasi pelet. Penelitian ini bertujuan agar mengetahui keefektifan Trichoderma  Formulasi pelet dalam menekan penyakit Layu Fusarium  yang disebabkan oleh Jamur Fusarium  oxysporum f.sp capsici. Penelitian ini telah dilaksanakan di laboratorium Penyakit Tumbuhan Program Studi Proteksi Tanaman dan Rumah Kasa Kebun Percobaan Fakultas Pertanian Unsyiah. Penelitian ini dimulai sejak bulan Agustus sampai Oktober 2017. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola non faktorial dengan 9 perlakuan dan 3 ulangan setiap perlakuan terdiri dari 5 unit polibag sehingga terdapat 135 unit polibag. Peubah yang diamati meliputi masa inkubasi, tinggi tanaman, jumlah daun dan persentase serangan.Hasil penelitian menunjukkan bahwa aplikasi pelet Trichoderma harzianum 2,5 g/polibagdan Trichoderma virens 2 g/polibag mampu memperlama masa inkubasi (Tidak terdapat gejala). Aplikasi pelet  T. virens 1,5 g polibag dapat meningkatkan tinggi tanaman cabai merah sampai 16, 80 cm. Aplikasi pelet T. harzianum  1,5 g/polibag dapat mamacu pertumbuhan  jumlah daun sebesar 9,07 helai. Aplikasi pelet T. harzianum 2,5 g/polibag dan T. virens formulasi pelet 2 g/polibag mampu menekan persentase serangan jamur Fusarium  oxysporum f.sp capsici sebesar 13,68 % pada 35 hari setelah tanam.Combination of Several Doses and Species of Trichoderma Pellet Formulations In Suppressing Development Fungus of fusarium oxysporum f.sp capsici in Red Chili Breeding (Capsicum annum L.)Abstract : a red chili (Capsicum annum L.) is one of the largest superior horticultural commodity of vegetables, because it has a high selling price. Although it has high price, but in the field red chilli often disturbance from the seed sowing until the pepper plants produce, it is cause by soil contagion or even carried by seed. One of the most common diseases in red chili plants is the wilting disease cause of the fungus Fusarium oxysporum f.sp capsici. It often detrimental of the farmers if the plants have been attacked by Fusarium wilt disease, the loss could reach out about 80% in red chili plants. One of the alternative to Integrated Pest and Disease Control (PHPT) is using the Trichoderma pellet formulation agent. The reasearch purpose to determine of the effectiveness Trichoderma Formulation of pellets in suppressing Fusarium Lung disease caused by Fusarium oxysporum f.sp capsici. The research has been conducted in Plant Disease Laboratory of Plant Protection Study Program and home srceen Experimental Garden of Agriculture Faculty Unsyiah. The research was started since August to October 2017. The research uses Completely Randomized Design (RAL) non factorial pattern with 9 treatments and 3 replications each treatment consists of 5 units of polybags so that there are 135 units of polybags. The variable observed included incubation period, plant height, number of leaves and percentage of attacks. Results of the research showed that the application of Trichoderma harzianum pellet 2.5 g / polybag and Trichoderma virens 2 g / polybag was able to prolong the incubation period. Application of T. virens pellet 1.5 g polybags can increase the height of red pepper plants up to 16, 80 cm. Application of pellets T. harzianum 1.5 g / polybags can spure growth of leaves amounted to 9.07 strands. The application of T. harzianum pellet 2.5 g / polybag and T. virens pellet 2 g / polybag formulation was able to suppress the percentage of Fusarium oxysporum f.sp capsici attack by 13.68% at 35 days after planting.

scholarly journals Non-Synergistic Effect of Trichoderma harzianum and Glomus spp. in Reducing Infection of Fusarium Wilt in Banana

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Arfe Castillo ◽  
Cecirly Puig ◽  
Christian Cumagun
Keyword(s):  
Biological Control ◽  
Synergistic Effect ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Trichoderma Harzianum ◽  
Root Weight ◽  
Biological Control Agents ◽  
Competitive Effects ◽  
Glomus Spp ◽  
Race 4

Philippine banana is currently threatened by Fusarium oxysporum f. sp. cubense Tropical Race 4 (FocR4). This study investigated the use of Trichoderma harzianum pre-treated with Glomus spp, as a means of managing Fusarium wilt on young ‘Lakatan’ banana seedlings. Results showed that Glomus applied basally significantly improved banana seedling growth with increased increment in plant height and pseudostem diameter and heavier root weight. The application of Glomus spp. alone offered 100% protection to the ‘Lakatan’ seedlings against FocR4 as indicated by the absence of the wilting symptom. A combination of T. harzianum and Glomus spp. also gave significant effect against Fusarium wilt through delayed disease progression in the seedlings but was not synergistic. Competitive effects were suspected when application of the two biological control agents on banana roots was done simultaneously.

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