scholarly journals AMINO ACID COMPOSITION AND ANTI-TYROSINASE ACTIVITY OF METABOLITES FROM EDIBLE Pleurotus SPECIES FOR THEIR NUTRITIONAL AND THERAPEUTIC POTENTIAL

2021 ◽  
Vol 9 (6) ◽  
pp. 837-850
Author(s):  
Iyabo O. Omomowo ◽  
Comfort O. Bamigboye ◽  
Olawale I. Omomowo ◽  
Olusola N. Majolagbe ◽  
Adijat F. Ogundola ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Value ◽  
Tyrosinase Activity ◽  
Environmental Study ◽  
Mushroom Tyrosinase ◽  
Inhibitory Effects ◽  
Pleurotus Species

Mushrooms are being extensively researched due to their nutritional value and medicinal importance. The genus Pleurotus is the second most cultivated mushroom and is known for its high nutritional value, therapeutic properties, taste, flavor, as well as their application in biotechnology and environmental study. Also, tyrosinase is prevalent in most living organisms. The enzyme catalyzes the oxidation of monophenols to ortho-quinones in a two-step reaction process. This study was aimed to assess the amino acid composition and anti-tyrosinase activity of metabolites obtained from edible Pleurotus species. Assessment of the nutritional content and inhibitory studies of mushroom tyrosinase produced from four Pleurotus strains was carried out using high-performance liquid chromatography (RP-HPLC). The results of the study showed that seventeen different amino acids were identified in the crude and partially purified protein metabolites. Also, the crude extract metabolite had the highest quantity of amino acids than the partially purified. The highest and lowest amino acids value Glutamic acid (1343.26 µmol/mL) and valine (0.34 µmol/mL). The anti-tyrosinase inhibition studies of the four Pleurotus strains showed varying results from significantly inhibitory effects to slightly inhibitory effects on mushroom tyrosinase. The highest inhibition was 14.86% (Pleu-W), while the lowest inhibition was 51.42% (Plof-30) respectively. The high point of this study is that the Pleurotus species contains a significant number of amino acids and also, they possess good anti-tyrosinase activity. Therefore, these are a good source of nutritional and therapeutic metabolites and these can be explored further for their nutritional and medicinal importance to man.

scholarly journals Пищевая ценность телятины как важнейший критерий оценки мясного сырья для детского питания

2018 ◽  
Vol 8 (1) ◽  
pp. 637-643
Author(s):  
T.L. Holubenko
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Value ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Baby Food ◽  
Black And White ◽  
Balanced Diet

<p><span lang="EN-US">The aim of the research is to assess the amino acid composition of veal from different genotype bull-calves concerning the usage in baby food production. A comparative analysis of the essential amino acids content in meat of calves of <span>black-and-white motley breed,</span> Aberdeen Angus breed and black and white crossbreeds grown according to the traditional technology of dairy cattle breeding hasn’t showed any significant differences. However, some differences were observed for each separate amino acid. Although the difference in amino acids was 2. 9% in favor of <span>black-and-white motley breed</span>. According to the amino acid composition, the calves meat of Charolais breed is biologically more complete than the Aberdeen Angus calves meat of the first generation in the valine content by 7.4%, isoleucine by 45.3% (P &lt;0.001), leucine by 15.2% (P &lt;0.001), lysine by 7.8%, threonine and phenylalanine + tyrosine by 6.5% (P &lt;0.05) and 7.5% (P &lt;0.01), respectively. Amino acid content was limited by the sum of amino acids phenylalanine + tyrosine (80.2%) in purebreds and methionine + cystine (83.4%) in <span>crossbreeds</span>. The other amino acids content was more than 100%; it indicates a high biological and nutritional value of veal. In the first experiment, the degree of compliance with the norms of a balanced diet is 37.4-38.2%. The human body's need for such essential amino acids as valine, isoleucine, phenylalanine + tyrosine is satisfied for more than 20%; the human body's need for leucine, lysine, and threoni9ne is satisfied for more than 30%. Veal of the Charolaise young is distinguished by higher indicators. The degree of its compliance with the norms of a balanced diet is 43.8% against 37.6% in hybrid calves. It is proved that veal obtained from young animals of different breeds in ecologically clean zones has a high biological and nutritional value, it corresponds to the indices for meat raw materials for baby food in accordance with Sanitary Norms 11-63 RB98.</span></p>

scholarly journals Nutritional value and amino acids composition of the mushroom (Agaricus bisporus) at different stages of its development

2013 ◽  
Vol 38 (2) ◽  
pp. 103-113 ◽  
Author(s):  
Józef Bąkowski ◽  
Ryszard Kosson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Agaricus Bisporus ◽  
Crude Protein ◽  
Nutritional Value ◽  
Chicken Manure ◽  
Total N ◽  
Stage 4

The study of the nutritional value and amino acid composition of the mushroom (<i>Agaricus bisporus</i>) was carried out with the Somycel 653 strain cultivated on a synthetic compost containing rye straw, broiler chicken manure and gypsum. In 4 developmental stages of the whole fruit-bodies and the caps and stipes the following were determined: dry matter, vitamin C, nitrates, total nitrogen, crude protein (N × 4.38) and amino acid composition. It was observed that in all stages of development the levels of total N and crude protein are significantly higher in the caps than in the stipes. From the amino acid composition it appears that the caps of stage 4 contain the highest amounts of essential and total amino acids as compared with caps, stipes and whole sporophores found in any developmental stage. This is correlated with the opening of the mushrooms in stage 4. It was observed that the nitrates level tends, to be higher in the cap than in the stipe.

scholarly journals Mineral and Amino Acid Composition of Germinated and Canned Wheat Grains

2019 ◽  
pp. 513-521
Author(s):  
Maria Zenkova
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Value ◽  
Wheat Grain ◽  
Biological Value ◽  
Wheat Grains ◽  
Mineral Substances ◽  
Technological Processing

Introduction. Grain germination can improve the nutritional value and functional properties of grain. The research featured the effect of technological processing on the quality of the finished product as in the case of natural, germinated, and canned wheat grains. A set of experiments was conducted to define the mineral substances and amino acid composition as factors that affect the chemical composition of a product during its production. Study objects and methods. The research featured soft wheat grain, germinated wheat grain, and canned germinated wheat grain. The latter consisted of 55% of pre-treated germinated wheat grain and 45% of filling, which contained 4.5% of sugar and 3.5% of salt. The content of mineral substances was determined by atomic-emission spectrometry with inductively coupled plasma using an atomicemission spectrometer Optima 2100 DV (USA). The amino acid composition was determined using a liquid chromatograph Agilent 1200 (USA). Results and discussion. 100 grams of canned germinated grain contained about 15% of daily manganese, 12% of chromium (III), 8.4% of copper, and 8.3% of zinc. Canned germinated wheat demonstrated a high biological protein value and contained 20.6% of essential amino acids. As for amino acids, canned germinated wheat grains had a high content of proline (958.4 mg/100 g) and glutamic acid (2456.4 mg/100 g). The characteristics of the nutritional value of canned protein from germinated wheat grains included amino acid rate, amino acid feedstock difference coefficient (AAFDC), and potential biological value (PBV). The proteins of canned germinated wheat appeared inferior in their amino acid composition and contained insufficient amounts of lysine and threonine. The coefficient of differences in amino acid scores was 71.9%, while the potential biological value of the protein was 28.1%. Conclusion. Germinated grains have a certain set of minerals and amino acids in their composition. Therefore, they can be used to develop canned foods or dishes for public catering industry. The effect of technological processing on the mineral composition of the finished product resulted in the loss of mineral substances from 45 to 85%. Moreover, germinated wheat should be combined with products that are rich in lysine and threonine.

scholarly journals Variability Russian Rice Varieties in Essential Amino Acids Composition

2021 ◽  
Vol 285 ◽  
pp. 02046
Author(s):  
Yulia Goncharova ◽  
Svetlana Verechagina ◽  
Nikolay Ochkas ◽  
Sergey Goncharov
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Value ◽  
Protein Composition ◽  
Essential Amino Acids ◽  
Rice Varieties ◽  
Amino Acids Composition ◽  
White Grain

Rice contains antioxidants, minerals, carbohydrates, lipids, vitamins that make it a functional food. A dozen times higher content of antioxidants was noted in black-grain and red-grain rice varieties as compared to white-grain ones. In terms of protein content, color and white-grain varieties do not differ significantly. The protein composition largely determines the nutritional value and functional properties, as well as the texture and taste of rice. Although rice contains less protein than many cereals, its biological value is highest due to balanced composition of amino acids. Nutritional value of different rice varieties varies greatly due to different amino acid composition. Determination of amino acid composition was carried out on devices Kapel 105 - M. In the work used 50 collection samples of rice. The variability was studied and the sources were identified among domestic rice varieties. To create a new generation varieties with more balanced amino acid composition, it is necessary both to isolate sources with a high content of individual amino acids, and varieties with high characteristics in terms of content complex of amino acids, especially essential ones.

scholarly journals Differences in the amino acid composition of the breast muscle of wild and farmed pheasants

2012 ◽  
Vol 30 (No. 4) ◽  
pp. 309-313 ◽  
Author(s):  
A. Brudnicki ◽  
A. Kułakowska ◽  
D. Pietruszyńska ◽  
M. Łożyca-Kapłon ◽  
J. Wach
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Value ◽  
Amino Acid Profile ◽  
Breast Muscle ◽  
High Quality ◽  
Nutritional Values ◽  
Biological Value

Numerous studies show the slaughter yield and also basic chemical composition of pheasant meat. The results reveal a higher biological value of the meat of pheasants which were fed naturally, in comparison to the meat of pheasants fed with commercial mixtures. In many countries, the pheasant is selected with the aim of producing high quality meat with very desirable nutritional values. There are only few publications on amino acid composition of pheasant meat. The knowledge of amino acid composition of pheasant meat can be used to determine its potential nutritional value. The amino acid compositions were compared of the meats of wild and farm pheasants. In the study, the following amino acids were determined: Asp, Thr, Ser, Glu, Pro, Gly, Ala, Val, Ile, Leu, Tyr, Phe, His, Lys, Arg. An improved amino acid profile was found in the breast muscle of pheasants kept at the farm in comparison with that of wild pheasants. &nbsp;

scholarly journals Effect of feed composition on the nutritional value of meat of African catfish

2020 ◽  
Vol 27 ◽  
pp. 00134
Author(s):  
L. A. Shadieva ◽  
E. M. Romanova ◽  
V. N. Lyubomirova ◽  
V. V. Romanov ◽  
T. M. Shlenkina
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Value ◽  
Fat Content ◽  
High Protein ◽  
Protein Diet ◽  
African Catfish ◽  
Feed Composition

The article outlines the results of the research into the influence of feed composition on the amino acid value of African catfish meat. It has been shown that quality characteristics of fish meat depend on protein and fat content. It has been proved that high-protein feed ensures increase in the content of all amino acids in African catfish meat. Nevertheless, protein and fat content in the muscles of the studied fish is more than 2 times higher than the same indicator in the fish on low-protein and low-fat diet. Meat of the African catfish is rich in two amino acids – leucine and lysine. Two amino acids, tryptophan and methionine, are limitative at a high protein diet. At a lower protein diet, isoleucine amino acid is also added. The amino acid composition of African catfish meat is highest at high-protein feeds. The amino acid index of African catfish muscles at high-protein feeds is 0.48, significantly exceeding the index of fish bred on feeds with a reduced protein content. The conducted studies have shown that the use of high-protein feeds in catfish breeding stimulates protein metabolism, enriching the amino acid composition of muscle tissue and increasing the nutritional value of fish as a food product. The research has been funded by the Russian Foundation for Fundamental Research, project No. 18-016-00127.

scholarly journals The Amino Acid Composition of Keratins

1955 ◽  
Vol 8 (4) ◽  
pp. 537 ◽  
Author(s):  
DH Simmonds
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Merino Wool

The amino acid composition of 16-hr 6N HCI hydrolysates of three qualities of commercially classified wools has now been determined using the technique of Moore and Stein (1951). In this paper the results obtained on samples of Merino 70's and Corriedale 56's wool are compared with those previously reported for Merino wool of 64's quality. The overall pattern of the amino acid composition of the three wools is similar although small variations between the wools are observed with some of the amino acids.

scholarly journals Amino acid production in isolated rat liver mitochondria

1973 ◽  
Vol 134 (2) ◽  
pp. 431-436 ◽  
Author(s):  
W. Ferdinand ◽  
W. Bartley ◽  
V. Broomhead
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Lipid Hydroperoxide ◽  
Liver Mitochondria ◽  
Cysteic Acid ◽  
Rat Liver Mitochondria ◽  
Isolated Mitochondria ◽  
Selective Retention

Amino acid analyses of mitochondrial membranes are compared with the amino acid composition of whole mitochondria (Alberti, 1964) and found to be very similar except in the cystine content. The composition of the endogenous amino acids found in freshly prepared mitochondria has been established and shown to differ considerably from the amino acid composition of membranes or whole mitochondria. The amino acids produced during anaerobic incubation of mitochondria at pH7.4, on the other hand, resemble the membrane in composition, supporting the view that neutral proteinase activity is responsible for their appearance. Aerobic incubation produces a similar pattern of amino acids except that amino acids such as proline, serine, asparagine, glutamic acid and glutamine, which can be metabolically utilized under aerobic conditions, are present to a smaller extent. The presence of large relative concentrations of endogenous taurine, cysteic acid and oxidized glutathione and the accumulation of taurine during incubation is found. The selective retention of taurine and cysteic acid within the mitochondria is established. It is proposed that the first step in the degeneration of isolated mitochondria results from lipid hydroperoxide accumulation caused by the lack of glutathione reductase in isolated mitochondria.

scholarly journals AMINO ACID COMPOSITION AND ELECTROPHORETIC PROPERTIES OF HYPERTENSIN I

1955 ◽  
Vol 102 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Leonard T. Skeggs ◽  
Walton H. Marsh ◽  
Joseph R. Kahn ◽  
Norman P. Shumway
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Amino Acid ◽  
Ion Exchange ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Isoelectric Point ◽  
Column Chromatography ◽  
Isoleucine Leucine ◽  
Ion Exchange Column

A preparation of hypertensin I was purified by countercurrent distribution and was shown to migrate as a single component in starch blocks at pH 9.3 and 4.2. It had an isoelectric point of 7.7. Quantitative analysis by ion exchange column chromatography showed eight amino acids in approximately unimolar proportion: aspartic, proline, valine, isoleucine, leucine, tyrosine, phenylalanine, and arginine. There were in addition two moles of histidine.

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