scholarly journals Dietary Supplementation of a Mixture of Saponin-Rich Plants to Reduce Ammonia-Nitrogen Excretion in Juvenile Nile Tilapia (Oreochromis niloticus)

2018 ◽  
Vol 2 ◽  
pp. 6-12
Author(s):  
Paul Engler ◽  
Pierre Caillis ◽  
Samuel Voller ◽  
Laura Labrie

The intensification in aquaculture production leads to increasing attention on the management of ammonia from its waste. Natural solutions such asYucca schidigeraextracts have shown beneficial effects. Their origin (harvested from the wild), however, call for alternative and equally efficient solutions in order to increase the sustainability of their production. In this work, juvenile Nile tilapias (Oreochromis niloticus) were supplemented with different doses of a commercial mixture of saponin-rich plants (Norponin® OPTI, CTL: 0ppm, D1: 100ppm, D2: 500ppm and D3: 2500ppm). The impact on water quality was monitored, with a particular attention to total ammonia excretion (TAN). TAN was significantly reduced in treatments D1 and D2 compared to the control and D3 treatments (p<0.05). Overall, the present study demonstrates that a supplementation with low doses of the feed additive (100 or 500ppm) induced beneficial effects on TAN management.

2017 ◽  
Vol 39 (5) ◽  
pp. 601
Author(s):  
Davi De Holanda Cavalcante ◽  
Francisco Roberto Dos Santos Lima ◽  
Vanessa Tomaz Rebouças ◽  
Marcelo Vinícius do Carmo e Sá

The present study aimed to assess the possible beneficial effects of the integration between bioflocs and periphyton to the Nile tilapia’s water quality and growth performance. There were four treatments with five replicates each: (1) Control: green waters, (2) Periphyton: substrate-based system, (3) BFT: bioflocs technology for aquaculture, and (4) Biophyton: integration between bioflocs and periphyton. Fish (1.63 ± 0.07 g) were reared for 10 weeks in twenty 250 L outdoor tanks. Two polyethylene boards were vertically set out in the Periphyton and Biophyton tanks as underwater substrates. The C: N ratios of water in the BFT and Biophyton tanks were adjusted to 15:1 with dry molasses applications. The concentrations of total ammonia nitrogen were higher in the Control and Periphyton tanks than in the BFT and Biophyton ones. On the other hand, the concentrations of reactive phosphorus were higher in the BFT and Biophyton tanks than in the Control and Periphyton ones. The fish final body weight, specific growth rate and fish yield have not differed between the tanks. The integration between bioflocs and periphyton has not brought clear benefits to tilapia culture on water quality and growth performance.  


2009 ◽  
Vol 296 (5) ◽  
pp. F994-F1005 ◽  
Author(s):  
M. H. Braun ◽  
S. L. Steele ◽  
M. Ekker ◽  
S. F. Perry

Injection of antisense oligonucleotide morpholinos to elicit selective gene knockdown of ammonia (Rhag, Rhbg, and Rhcg1) or urea transporters (UT) was used as a tool to assess the relative importance of each transporter to nitrogen excretion in developing zebrafish ( Danio rerio). Knockdown of UT caused urea excretion to decrease by ∼90%, whereas each of the Rh protein knockdowns resulted in an ∼50% reduction in ammonia excretion. Contrary to what has been hypothesized previously for adult fish, each of the Rh proteins appeared to have a similar effect on total ammonia excretion, and thus all are required to facilitate normal ammonia excretion in the zebrafish larva. As demonstrated in other teleosts, zebrafish embryos utilized urea to a much greater extent than adults and were effectively ureotelic until hatching. At that point, ammonia excretion rapidly increased and appeared to be triggered by a large increase in the mRNA expression of Rhag, Rhbg, and Rhcg1. Unlike the situation in the adult pufferfish ( 35 ), the various transporters are not specifically localized to the gills of the developing zebrafish, but each protein has a unique expression pattern along the skin, gills, and yolk sac. This disparate pattern of expression would appear to preclude interaction between the Rh proteins in zebrafish embryos. However, this may be a developmental feature of the delayed maturation of the gills, because as the embryos matured, expression of the transporters in and around the gills increased.


2017 ◽  
Vol 9 (2) ◽  
pp. 429-441
Author(s):  
Wastu Ayu Diamahesa ◽  
Toshiro Masumoto ◽  
Dedi Jusadi ◽  
Mia Setiawati

Efforts to reduce the impact of waste improvement on degradation of water quality can be transferred by utilization of inorganic waste as a source of seaweed nutrition. This study aimed to determine the growth and protein content of Ulva prolifera maintained at different flow rates in integrated aquaculture system. 9 Yellowtail stocked with 5.095 g with an average weight of 566.11±81.51 g were kept in 540 L tank for 24 days, by water flowing at the rate of 10 L min-1. Water from the fish tank was distributed into the sediment tank and go to 6 Ulva tanks with the flow rate of 0.5, 1.0 and 1.5 L min-1. Test parameters measured were growth performance of Yellowtail, biomass of Ulva prolifera, protein content of Ulva prolifera, and total ammonia nitrogen. The measurement results showed that the biomass of fish increased to 5.408 g, then biomass of Ulva increased to 42 g, 156 g and 155 g for flow rate of 0.5 L, 1 L and 1.5 L min-1, respectively. The protein content of Ulva for all the treatments was the same (P> 0.05). A total of ammonia in the tank outlet of Ulva (0.0202 - 0.1137 mg N L-1) were smaller than those were in the inlet (0.0286 - 0.1394 mg N L-1).


Author(s):  
M. Lazarevic ◽  
R. Resanovic ◽  
I. Vucicevic ◽  
A. Kocher ◽  
C.A. Moran

SummaryAmmonia production in commercial poultry sheds poses a problem for farmers as high levels can affect animal's respiratory health, resulting in hock and foot pad lesions and thereby affecting performance. A study was conducted to evaluate the suitability of whole, chopped and dried Yucca schidigera (De-Odorase™, Alltech Inc, USA) to reduce ammonia excretion from broilers and investigate the impact on broiler performance. Nine hundred and two, one-day-old Cobb broilers were randomly assigned to one of two diets, without (control) or supplemented with 500 g/t De-Odorase™. Birds fed the treatment diet had significantly higher body weight (P < 0.05) at 42 d and improved FCR (P < 0.05). Significant reductions in ammonia from litter were reported at 37 and 42 days old (P < 0.05 and P < 0.1, respectively). No significant differences were seen in foot pad, gait score, feather score or carcass data; although the birds fed De-Odorase™ had consistent numerical improvements in these parameters. The results of this study suggest that feeding whole, chopped and dried Yucca schidigera as a functional ingredient in the diet can be part of an ammonia management programme to improve broiler welfare and zootechnical performance.


1994 ◽  
Vol 190 (1) ◽  
pp. 179-193 ◽  
Author(s):  
D Varley ◽  
P Greenaway

The rate and mechanism of nitrogen excretion were examined in Geograpsus grayi. This species excretes waste nitrogen as gaseous NH3 in periodic bursts. The mean concentration of total ammonia ([NH3]+[NH4+]) in the primary urine during bursts of excretion (1.72 mmol l-1) was similar to that of haemolymph (2.07 mmol l-1) but was significantly lower (P&lt;0.005) than that of branchial fluid (80.6 mmol l-1). The effects of ion exchange inhibitors on the apical membrane of the gill epithelium in Geograpsus grayi were examined. The presence of an amiloride-sensitive Na+/NH4+ exchanger was confirmed and a SITS-sensitive Cl- influx suggested Cl-/HCO3- exchange. Thus, the site of nitrogenous excretion in this species is the branchial chamber, which is also the site of reprocessing of urine for ion regulation in other terrestrial crabs. Gaseous ammonia excretion is achieved by volatilisation of NH3 from the branchial fluid. High partial pressures of ammonia in the branchial fluid are produced by apical Na+/NH4+ exchange and elevation of the pH.


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