Falls risk is predictive of dysphagia in Parkinson’s disease

Objective Evaluate the relationship between falls, freezing of gait, and swallowing disturbance in Parkinson’s disease (PD). Background Dysphagia is a common symptom in PD, and is often thought of as an axial feature along with falls and gait disturbance. It is of interest to examine the relationship between these symptoms in PD, given the possibility of shared pathophysiology due to non-dopaminergic and extranigral dysfunction. Methods We recruited 29 consecutive non-demented patients with idiopathic PD and at least one clinically determined impairment in swallowing, falls, or freezing of gait. Swallow dysfunction was assessed using the Swallowing Disturbance Questionnaire (SDQ). The Falls Efficacy Scale and Freezing-of-gait questionnaire were recorded. Correlation analysis and multiple regression were used to determine the relationship between swallow and gait disturbance. Results Total SDQ score correlated strongly with the falls efficacy scale (Spearman’s rho = 0.594; P = 0.001), but not with the freezing-of-gait score. Linear regression controlling for other factors associated with dysphagia identified falls efficacy score as a significant predictor of swallow dysfunction. Conclusions The severity of dysphagia in PD is closely related to severity of falls, but not gait freezing. This may be helpful to more precisely determine the anatomical substrate of levodopa-resistant axial symptoms in PD and provide clues to further management.

Effect of Feeding a High Calcium: Phosphorus Ratio, Phosphorous Deficient Diet on Hypophosphatemic Rickets Onset in Broilers

Recently, a P-deficient diet caused rickets in commercial chicks within three days. This study aimed to investigate the duration of onset of rickets in chicks. Data were collected from 3–11 day old chicks raised on 88 commercial farms. Male day-old Arbor Acres Plus broilers (n = 450) were studied in three trials, with three to four treatments each. Each treatment used one of the following crumbled feeds: control feed (calcium (Ca): phosphorus (P)-1.41), slightly high Ca:P feed (SHCa:P, Ca:P-2.69), high Ca:P ratio, P deficient feed (HCa:P, Ca:P-3.08), and HCa:P feed plus 1.5% dicalcium phosphate (HCa:P + DP). Each treatment had three replicates with 15 birds each. Rickets was induced by HCa:P, and cured by HCa:P + DP, confirmed by gross anatomy, gait score, serum P concentration and growth performance. Lameness was not found in control groups, whereas, observed in the HCa:P groups as early as day 2.7 on commercial farms and day 3 in experimental farm. Serum P was reduced in HCa:P (p < 0.01). Bodyweight and feed intake started decreasing at day 3 on commercial farms and in all trials (p < 0.01). The duration of onset of hypophosphatemic rickets in broiler chicks fed HCa:P crumbled feed is approximately three days.

Movement Analysis to Associate Broiler Walking Ability with Gait Scoring

The genetic development of the commercial broiler has led to body misconfiguration and consequent walking disabilities, mainly at the slaughter age. The present study aimed to identify broiler locomotion ability using image analysis automatically. A total of 40 broilers that were 40 d old (male and female) were placed to walk on a specially built runway, and their locomotion was recorded. An image segmentation algorithm was developed, and the coordinates of the bird’s center of mass were extracted from the segmented images for each frame analyzed, and the unrest index (UI) was applied. We calculated the center of mass’s movement of the broiler walking lateral images capturing the bird’s displacement speed in the onward direction. Results indicated that broiler walking speed on the runway tends to decrease with the increase of the gait score. The locomotion did not differ between males or females. The proposed algorithm was efficient in predicting the broiler gait score based on their displacement speed.

Movement Analysis to Predict Broiler Walking Ability

The genetic development of commercial broiler led to body misconfiguration and consequent walking disabilities, mainly at the slaughter age. The present study aimed to identify broiler locomotion ability using image analysis automatically. A total of 40 broiler 40 d-old were placed to walk on a specially built runway, and their locomotion was recorded. An image segmentation algorithm was developed, and the coordinates of the bird's center of mass were extracted from the segmented images for each frame analyzed, and the Unrest Index (UI) was applied. We calculated the center of mass's movement of the broiler walking's lateral images, therefore, capturing the bird's displacement speed in the onward direction. Results indicated that broiler speed on the runway tends to decrease with the increase of the gait score. The locomotion did not differ between males or females. The proposed algorithm was efficient if predicting the broiler gait score based on their displacement speed.

Effect of Hedyotis diffusa Willd extract on gouty arthritis in rats

Purpose: To investigate the effect of Hedyotis diffusa Willd extract (HDWE) on gouty arthritis in rats.Method: Monosodium urate (MSU) crystal was injected into the ankle joint of rats to establish a rat model of gouty arthritis. HDWE (4.8, 9.6 and 19.2 g/kg) was administered to the rats treated with MSU crystals. The walking behavior of the rats was observed daily, and the gait score was calculated to evaluate the Oswestry disability index of rats. Levels of IL-1β and TNF-α in lavage fluid of articularcavities were measured using enzyme linked immunosorbent assay (ELISA) kits. The synovial tissues of joint of control, model and 19.2 g/kg HDWE group rats were obtained and NLRP3 inflammasome was analysed by Western blot.Results: The results showed that HDWE ameliorated the symptoms of gouty arthritis and gait score in rats significantly (p < 0.05). Further pharmacological experiments showed that all doses of HDWE decreased the levels of inflammatory cytokines IL-1β and TNF-α (p < 0.05), and inhibited NLRP3, caspase-1, ASC, IL-1β and IL-18 protein expressions of the lavage fluid of articular cavities in MSU crystal-treated rats (p < 0.01).Conclusion: The results indicate that HDWE exhibits a significant effect in ameliorating gouty arthritis via inhibition NLRP3 inflammasome, and thus is a potential new drug choice for the treatment of gouty arthritis. Keywords: Hedyotis diffusa, Caspase, Gouty arthritis, Inflammatory cytokines, NLRP3 inflammasom

Freezing of Gait in Parkinson’s Disease: Risk Factors, Their Interactions, and Associated Nonmotor Symptoms

Background. Freezing of gait (FOG) is a debilitating and incompletely understood symptom in Parkinson’s disease (PD). Objective. To determine the principal clinical factors predisposing to FOG in PD, their interactions, and associated nonmotor symptoms. Methods. 164 PD subjects were assessed in a cross-sectional retrospective study, using the MDS-UPDRS scale, MMSE, and Clinical Dementia Rating Scale. Clinical factors associated with FOG were determined using univariate analysis and nominal logistic regression. Receiver operating characteristic curves were computed, to obtain measures of sensitivity and specificity of predictors of FOG. Subgroups of patients with FOG were compared with those without FOG, based on defining aspects of their clinical phenotype. Results. Relative to non-FOG patients, those with FOG had a longer disease duration, higher PIGD and balance-gait score, higher LED, and more motor complications ( p < 0.0001 ) and were more likely to exhibit urinary dysfunction ( p < 0.0003 ), cognitive impairment, hallucinations, and psychosis ( p = 0.003 ). The balance-gait score and motor complications, at their optimum cutoff values, together predicted FOG with 86% accuracy. Interactions were noted between cognitive dysfunction and both the Bal-Gait score and motor complication status, cognitive impairment or dementia increasing the likelihood of FOG in subjects without motor complications ( p = 0.0009 ), but not in those with motor complications. Conclusions. Both disease and treatment-related factors, notably LED, influence the risk of FOG in PD, with a selective influence of cognitive dysfunction in patients with balance-gait disorder but not in those with motor fluctuations. These findings may help to inform clinical management and highlight distinct subgroups of patients with PD-FOG, which are likely to differ in their network pathophysiology.

Gait Score, Latency-to-Lie and their Association with Body Weight and Morphometric Measurements in Light and Heavy Genotypes of Domestic Turkey

Locomotion problems limit access to drinkers and feeders with a resultant effect on birds' survival and productivity. In this study, gait score (GS), latency-to-lie (LTL) and their association with body weight (BWT) and morphometric traits in light weight (Nigerian Local, NL) and heavy weight (Nicholas White, NW) strains of turkeys were evaluated. A total of two hundred day-old Nigerian local and Nicholas white poults were used for the study. Gait of poults were scored at 4, 8, 12 and 16 weeks of age using Kestin gait scoring system. Other parameters measured include latency-to-lie, body weight and morphometric traits at 4, 8, 12 and 16 weeks of age. The results showed that poults with normal walking ability (GS=0) in the light strain Nigerian local declined slightly from 88.4 percent at week 4 to 85.0 percent at week 16, while poults with normal walking ability (GS=0) in Nicholas white declined from 81.1 at week 4 to 16.4 percent at week 16, respectively. The GS of Nicholas White was higher (p<0.05) than that of NL at 8 and 16 weeks of age. The LTL values decreased (1310.1- 555.7 seconds) with the age of birds and were higher (p<0.05) in NL at 8, 12 and 16 weeks of age. Correlation (r =-0.256 to 0.278) between BWT and GS was significant (p<0.05) at weeks 8 and 12 in NW and at weeks 12 and 16 in NL. BWT was correlated (p<0.05) with body girth, keel length, femur length, shank diameter and Tibia Metatarsus in both genotypes. There was significant correlation (p<0.05) between LTL and GS (r= -0.36 to -0.87), BWT and LTL (r= -0.24 to 0.31), BWT and LM traits (r= 0.56 to 0.96) in both genotypes. It was concluded that GS and LTL are influenced by genotype and age of poults.

Effect of various monochromatic light-emitting diode colours on the behaviour and welfare of broiler chickens

This study was conducted to evaluate the effect of different monochromatic light-emitting diode colours on the behaviour and welfare of broiler chicks. A total of 750 one-day-old chicks were used and lighting was set up as follows: pure blue (PB, 440–450 nm), bright blue (460–470 nm), sky blue (480–490 nm), greenish blue (500–510 nm), and green (530–540), while fluorescent white (400–700 nm) was used as a control. Birds were placed into 30 independent light proof pens and each light treatment was replicated five times with 25 birds in each pen. Video was recorded and behaviour was evaluated twice per day and observed five consecutive days in a week. Broiler welfare was evaluated using the characteristics of gait score, tibia dyschondroplasia, tonic immobility duration, and heterophil:lymphocyte ratio. In results, sitting, walking, and ground pecking behaviour were influenced by the light colour from 0 to 7 d. Extending the rearing period from 8 to 21 d resulted in increased sitting behaviour and decreased walking and pecking behaviour in chicks in the PB treatment (P < 0.05). When the growth period was extended further (22–42 d), sitting behaviour increased when chicks were exposed to PB light (P < 0.05). The effect of light colour did not significantly influence welfare of broiler chicks. Thus, the present results suggest that PB light colour decreased broiler chickens movement and thus increased duration of sitting behaviour. These results would be helpful to choose light colour for broiler producers.