scholarly journals Transplanting Space Effect on In-vitro Raised Sugarcane

2018 ◽  
Vol 10 (01) ◽  
pp. 12-16
Author(s):  
Sonali Gangwar ◽  
Saurabh Pathak ◽  
Maya Datt Joshi
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth And Yield ◽  
Cane Yield ◽  
Sugarcane Variety ◽  
Rapid Multiplication ◽  
Space Effect ◽  
Healthy Seed ◽  
Disease Free

Tissue cuture is considered to be a best technique for rapid multiplication and production of disease free, healthy seed cane. With a view to studying the effect of transplanting spacing on growth and yield of micropropagated crop of sugarcane, an experiment was carried out at Tissue Culture Laboratory, Shobhit University, Meerut. Tissue culture raised plantlets of sugarcane variety. In vitro cultured sugarcane were transplanted at various spacing of 90 x 45, 90 x 60, 90 x 90 and 120 x 60 cm. Among the four spacings, the highest plant growth, number of tillers, number of malleable canes, cane height and cane yield were recorded at 90 x 60 cm. Thus, a spacing of 90 x 60 cm was found most suitable for transplantation of tissue culture raised plantlets of sugarcane.

Transplanting Space Effect on In-vitro Raised Sugarcane

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Sonali Gangwar ◽  
Saurabh Pathak ◽  
Maya Datt Joshi
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth And Yield ◽  
Cane Yield ◽  
Sugarcane Variety ◽  
Rapid Multiplication ◽  
Space Effect ◽  
Healthy Seed ◽  
Disease Free

Tissue cuture is considered to be a best technique for rapid multiplication and production of disease free, healthy seed cane. With a view to studying the effect of transplanting spacing on growth and yield of micropropagated crop of sugarcane, an experiment was carried out at Tissue Culture Laboratory, Shobhit University, Meerut. Tissue culture raised plantlets of sugarcane variety. In vitro cultured sugarcane were transplanted at various spacing of 90 x 45, 90 x 60, 90 x 90 and 120 x 60 cm. Among the four spacings, the highest plant growth, number of tillers, number of malleable canes, cane height and cane yield were recorded at 90 x 60 cm. Thus, a spacing of 90 x 60 cm was found most suitable for transplantation of tissue culture raised plantlets of sugarcane

scholarly journals Comparative studies on growth and Yield of Conventional and Tissue culture plants of Turmeric (Curcuma longa) var. CO2

2019 ◽  
Vol 14 (2) ◽  
pp. 162-165
Author(s):  
Chitra R
Keyword(s):  
Tissue Culture ◽  
Agronomic Traits ◽  
Curcuma Longa ◽  
Growth And Yield ◽  
Yield Potential ◽  
Planting Material ◽  
Cropping Season ◽  
Planting Materials ◽  
Disease Free

Turmeric (Curcuma longa L.) is an ancient spice, native of India and South East Asia used from antiquity as spice and a dye. It is commonly propagated through rhizomes. The availability of disease free quality planting material is scarce during the cropping season (June – September). An experiment was conducted to study the performance of in vitro derived turmeric plants with conventional rhizome under field condition. The results indicated that the tissue culture plants showed better performance over the conventional rhizome planting. Tissue culture plants grew vigorously and taller than conventional type. The highest yield potential was observed in tissue cultureplants (40.83 tons/ha) as compared to the conventional rhizome planting (30.14 tons/ha). The rhizome rot incidence was lower (3.87% ) in tissue culture plants than rhizome-derived plants (25.58% ). However, the agronomic traits observed during the present study in tissue culture plants are stable and rhizome harvested from tissue culture plants can be used as disease free planting materials for further planting.

RAPID IN VITRO PROPAGATION OF WESTERN GHATS CULTIVAR - COLOCASIA ESCULENTA FOR THE HIGH FREQUENCY OF PLANTLETS

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Jyothi R ◽  
Srinivasa Murthy K M ◽  
Hossein . ◽  
Veena .
Keyword(s):  
Tissue Culture ◽  
Wide Distribution ◽  
Colocasia Esculenta ◽  
Limiting Factor ◽  
Promising Alternative ◽  
Rapid Multiplication ◽  
Medical Plant ◽  
Total Fat ◽  
Planting Materials

Colocasia esculenta is commonly known as Taro, it is referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Every 100 g of taro corms possess 112 Kcal, 26.46 g carbohydrate, 1.50 g protein, 0.20 g total fat and 4.1g fiber (USDA National Nutrient Data Base). Besides its nutritional value, taro is used as a medical plant and provides bioactive compounds used as an anti-cancer drugs. Traditionally, cocoyams are vegetative propagated from tuber fragments, a practice that encourages pathogen distribution. Colocasia esculenta is a widely distributed food crop in the humid tropics and subtropics. Despite of its wide distribution, Taro plants are commonly infected with DsMV and other pathogens. This virus induces conspicuous mosaic, malformation, dwarfing or feathering on leaves in taro. As the results of infection, it reduces the quality and yield of taro production greatly. This virus is thus considered as a major limiting factor in the production of taro. Here plays the importance of  tissue culture plays a major role in producing the disease resistant plants round the year with high quality. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. KEYWORDS: Colocasia esculenta, Virus, Pathogens, Conventional propagation, Micropropagation, Yield, Rapid multiplication, Quality

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals Crude Citric Acid of Trichoderma asperellum: Tomato Growth Promotor and Suppressor of Fusarium oxysporum f. sp. lycopersici

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 222
Author(s):  
Abdulaziz A. Al-Askar ◽  
WesamEldin I. A. Saber ◽  
Khalid M. Ghoneem ◽  
Elsayed E. Hafez ◽  
Amira A. Ibrahim
Keyword(s):  
Citric Acid ◽  
Plant Growth ◽  
Organic Acid ◽  
Wilt Disease ◽  
Growth And Yield ◽  
Total Phenolic ◽  
Growth Promoters ◽  
Chlorophyll Pigments

Presently, the bioprocessing of agricultural residues to various bioactive compounds is of great concern, with the potential to be used as plant growth promoters and as a reductive of various diseases. Lycopersiconesculentum, one of the most consumed crops in the human diet, is attacked by Fusarium wilt disease, so the main aim is to biocontrol the pathogen. Several fungal species were isolated from decayed maize stover (MS). Trichodermaasperellum was chosen based on its organic acid productivity and was molecularly identified (GenBank accession number is MW195019). Citric acid (CA) was the major detected organic acid by HPLC. In vitro, CA of T.asperellum at 75% completely repressed the growth of Fusariumoxysporum f. sp. lycopersici (FOL). In vivo, soaking tomato seeds in CA enhanced the seed germination and vigor index. T. asperellum and/or its CA suppressed the wilt disease caused by FOL compared to control. There was a proportional increment of plant growth and yield, as well as improvements in the biochemical parameters (chlorophyll pigments, total phenolic contents and peroxidase, and polyphenol oxidase activities), suggesting targeting both the bioconversion of MS into CA and biological control of FOL.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals Organogenesis in okra (Abelmoschus esculentus L. Moench.): A plant recalcitrant to tissue culture

2016 ◽  
Vol 41 (3) ◽  
pp. 521-528
Author(s):  
MR Kabir ◽  
S Ahmed ◽  
MAY Akhond
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Growth ◽  
Root Induction ◽  
Ms Medium ◽  
Natural Conditions ◽  
Hypocotyl Explants ◽  
Cotyledonary Nodes ◽  
Dichlorophenoxy Acetic Acid

Seedling-derived cotyledonary nodes and hypocotyl explants of BARI Dherosh- 1 were cultured in vitro on MS medium supplemented with varying concentrations of 2, 4-Dichlorophenoxy acetic acid (2, 4-D), 6- Benzylaminopurine (BAP), Thidiazuron (TDZ), BAP with 1-Nepthaleneacetic acid (NAA), BAP with Indole 3-butyric acid (IAA) and Zeatin with IAA along with a control. Shooting response (100%) with callus was only observed from cotyledonary nodes on thidiazuron (TDZ) where hypocotyls produced only callus or callus with roots on different concentrations of plant growth regulators. Considering the shooting response, the cotyledonary nodes of BARI Dherosh-1 were cultured on various concentrations of TDZ for regeneration. The highest percentage (64.0) with maximum number (6.8) of shoots per explant were observed in 0.044 ?M TDZ in 8.4 days. The regenerated shoots were rooted on ½ strength MS, MS supplemented with 2.46 ?M IBA and 0.53 ?M NAA. The highest percentage (83.3) and minimum days (9.7) required for root induction were recorded in 2.46 ?M IBA. The rooted plantlets were transferred to soil and hardened in the plastic pots under green house conditions. The rooted shoots grew normally under natural conditions following acclimatization.Bangladesh J. Agril. Res. 41(3): 521-528, September 2016

scholarly journals Exploring the Potential Soil Bacteria for Sustainable Wheat (Triticum aestivum L.) Production

2019 ◽  
Author(s):  
Rizwan Sheirdil ◽  
Rifat Hayat ◽  
Xiao-Xia Zhang ◽  
Nadeem Abbasi ◽  
Safdar Ali ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Plant Growth ◽  
Triticum Aestivum L ◽  
Growth And Yield ◽  
Recommended Dose ◽  
Bacterial Strains ◽  
Chemical Fertilizers ◽  
Inorganic Fertilizers

Plant growth promoting rhizobacteria (PGPR) are capable to reduce the use of chemical fertilizers input cost of farmer. Keeping in view the study was designed to investigate and evaluate inoculation effect of indigenous rhizospheric bacteria on growth and yield of wheat (Triticum aestivum L.) under in vitro and in vivo conditions using different treatments. Ten potential strains were selected on the basis of their ACC deaminase activity, siderophore production, P-solubilization and production of indole acetic acid (IAA). Further these strains were tested in three different experiments (growth chamber, pot and field). We found significant increase in crop growth response to the inoculants in comparison with un-inoculated control. In pot and field trial we tested PGPR with recommended dose of inorganic fertilizers. The results of present study revealed that inoculation of bacterial strains with wheat seeds significantly increased plant growth and improved crop yield. Results of present study reveal that these strains could be employed in different combinations and can get higher yield in case of half recommended doses of inorganic fertilizers along with consortium of strains in comparison with sole application of recommended dose of fertilizer and with consortium of strains. These strains were further identified by 16Sr RNA gene sequencing, fatty acid profile and biolog. It can be concluded that inoculated bacteria have more potential and contributes in good crop quality, increased yield when they are applied in combination, thus have potential to minimize use of chemical fertilizers.

scholarly journals 664 PB 104 AGROBACTERIUM INFECTION OF WHITE ASH (FRAXINUS AMERICANA L.)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 528a-528
Author(s):  
Sharon A. Bates ◽  
John E. Preece ◽  
John H. Yopp
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth Regulators ◽  
Callus Formation ◽  
High Humidity ◽  
Ms Medium ◽  
Gall Formation ◽  
White Ash ◽  
Seedling Explants

Both greenhouse-grown white ash plants derived from tissue culture and rooted microshoots in high humidity trays were inoculated with 11 tumor-inducing Agrobacterium strains. Eight strains stimulated mutative gall formation. Plants inoculated with strain A281 exhibited a higher frequency of callus formation (greenhouse-22.2%; microshoots-18.8%) than other strains at the site of the wound. Therefore, strain A281 was used to inoculate seed and seedling explants in vitro. Explants were placed on MS medium containiner no plant growth regulators and inoculated at 0, 3, 5, 7, or 10 days after initiation. Plants inoculated at 10 days showed a higher frequency of callus formation (16.4%) than with earlier inoculations. Also, rewounding of the explant at inoculation resulted in a higher frequency of callus formation (11.3%) compared to not rewounding the explant (3.9%).

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