scholarly journals Isolation, identification and antibiotic sensitivity pattern of aerobic bacteria from burn wound patient admitted in tertiary care hospital

2020 ◽  
Vol 8 (6) ◽  
pp. 2095
Author(s):  
Amit Kumar Sah ◽  
Varun Goel ◽  
Siddu Lagamappagol
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
Infectious Complications ◽  
Aetiological Factor ◽  
Research Centre ◽  
Care Hospital ◽  
Burn Wound ◽  
Sensitivity Pattern

Background: Significant burn injuries induce a state of immunosuppression that predisposes patients to infectious complications, thus the rate of nosocomial infections are higher. Rapidly merging multidrug resistant among the various isolate in indoor burn patients are depending on time-line becoming serious threat for managing therapeutically. Objective of this study is to determine the aetiological factor, prevalence, antimicrobial susceptibility pattern and emerging nosocomial pathogens.Methods: A prospective study was carried in burn ward of K.L.E.’s Dr. Prabhakar Kore Hospital and Medical Research Centre, Belgaum for the period of 1 year. Pair of wound swab were collected from patient having burnt more than 30% (RULE OF NINE) on 3rd day of stay. Sample were collected aseptically from 30 patients and processed by convectional culture and biochemical identification procedures and tested against commonly used antibiotics.Results: 30 patients that fall under inclusive criteria were enrolled in the study. The total burn surface area (TBSA) ranges from 30-82%. The ratio of female to male patient suffering burn wound in our study is 1.5:1. Aetiology of burn is heat (moist/dry) mostly. Depending upon degree of burn, most of patient suffered from 20 degree (superficial to deep) injury. From 30 swab cultures, 42 isolates were identified during the study in which mixed were 66.66% and one is fungi. The most commonly isolated is Pseudomonas aeruginosa (45.24%) then Klebsiella pneumoniae (19.04%), Acinetobacter spp. (14.28%), Staphylococccus aureus (11.90%). Among gram positive isolates, isolates are found to be most resistant to Erythromycin (100%) and Co-trimoxazole (100%) and sensitive to Vancomycin (71.42%). Among gram negative isolates are found to be most resistant to Gentamicin (91.65%), Ciprofloxacin (82.35%), Ceftazidime (82.35%) and sensitive to Meropenem (52.95%), Piperacillin (35.30%), Carbenicillin (29.41%).Conclusions: Pseudomonas aeruginosa was found to be the most common isolate. The nature of microbial wound colonization and flora changes with time should be taken into consideration in empirical antimicrobial therapy.

scholarly journals Bacteriological profile with antibiotic sensitivity pattern of burn wound infections in a peripheral tertiary care hospital

2021 ◽  
Vol 8 (4) ◽  
pp. 1253
Author(s):  
Arnab Mandal ◽  
Swapan Das
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Susceptibility ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Specific Pattern ◽  
Care Hospital ◽  
Wound Infections ◽  
Burn Wound ◽  
Mortality And Morbidity ◽  
Sensitivity Pattern

Background: India, has an estimated burn incidence of 6-7 million annually. Nearly 10% of these are life threatening and require hospitalization, and main cause of mortality and morbidity of these burn patients were wound infection and sepsis after 1st 24 hours. The present study was tried to determine specific pattern of burn wound infections, and antibiotic susceptibility of those isolates.Methods: After matching inclusion and exclusion criteria, total 55 patients were taken for this institution based, prospective observational study. Wound swabs were collected on day 7 and cultured aerobically in MacConkey agar and 5% blood agar and antibiotic susceptibility testing was done on Muller Hinton agar using Kirby-Bauer disc diffusion method.Results: Among study population 69.1% patients were female and majority (29.1%) of the patients belongs to age group from 21 to 30. It was found that 48 wound swabs were positive for microorganisms, of which Pseudomonas aeruginosa was most common isolated organism (23.6%), followed by Klebsiella pneumoniae (16.4%) and Staphylococcus aureus (14.5%). The most effective antibiotic found in this study was piperacillin/tazobactam, followed by imipenem/cilastatin.Conclusions: It was seen that gram-negative organisms were more prevalent. Pseudomonas aeruginosa was the most common microorganism and piperacillin/tazobactam was most effective antibiotic.

PHENOTYPIC DETECTION OF EXTENDED SPECTRUM BETA LACTAMASE AND THEIR ANTIBIOTIC SENSITIVITY PATTERN IN VARIOUS CLINICAL ISOLATES OF ENTEROBACTERIACEAE

2021 ◽  
pp. 54-58
Author(s):  
Deepali Gupta ◽  
Vipin Kumar Varshney ◽  
Shachi Shachi
Keyword(s):  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Research Centre ◽  
Care Hospital ◽  
Beta Lactamase ◽  
Klebsiella Species ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Sensitivity Pattern ◽  
Esbl Producers

Extended-spectrum β-lactamases (ESBLs) are a group of plasmid-mediated, diverse, complex and rapidly evolving enzymes that are posing a major therapeutic challenge today in the treatment of hospitalized and community-based patients. Enterobacteriaceae group is the main cause of bacterial infection and in this family Escherichia coli and Klebsiella species are the most common causes of nosocomial infections. ESBLs represents a major threat among multidrug-resistant (MDR) bacteria isolates. These ESBL producing pathogens are now recognized globally as major causes of nosocomial and community-acquired infections. ESBL detection is important from a therapeutic point of view and for infection control purposes. Carbapenems are often used to treat infections caused by ESBL producing E. coli and Klebsiella. This study was conducted in indoor patient department of Teerthanker Mahaveer Medical College & Research Centre, Moradabad, U.P., which is a tertiary care hospital. This study was conducted during the period from April 2015 to December 2017.A total no. of 350 gram negative Enterobacteriaceae were isolated in 800 no. of samples. They were screened for the beta-lactamase production. Among the 350 isolates 210 strains were ESBL producers. The major ESBL producers were E.coli (32%) followed by Klebsiella, Enterobacter, Citrobacter, Proteus. Multidrug resistance to Fluoroquinolones and Aminoglycosides were observed in the ESBL producing organism. The most common ESBL producing organism were from ICU.

scholarly journals Molecular characterization of strong biofilm producing MDR, XDR and PDR Pseudomonas aeruginosa isolated from a tertiary care hospital in South India

2019 ◽  
Author(s):  
Bhavani Manivannan ◽  
Chanakya Pachipulusu ◽  
Balaram Khamari ◽  
Chandreyee Datta ◽  
Manmath Lama ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Tap Water ◽  
Random Amplified Polymorphic Dna ◽  
Phylogenetic Analyses ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Control Measures ◽  
Care Hospital ◽  
Sensitivity Pattern

Abstract Background : Pseudomonas aeruginosa is an opportunistic, gram negative bacterium that causes serious infections, especially among immunocompromised patients. An unusually high incidence of nosocomial P. aeruginosa infections was observed among patients hospitalized at Sri Sathya Sai Institute of Higher Medical Sciences, Puttaparthi, India, between 2014 November and 2015 February. Some of the patients being treated for a variety of cardiac and urological disorders in the Cardiothoracic and Vascular Surgery - Intensive Care Unit (CTVS-ICU) and Urology ward were found infected with P. aeruginosa . Active surveillance and environmental sampling revealed the presence of two additional MDR P. aeruginosa in the tap waters of CTVS-ICU. Based on the Antibiotic sensitivity pattern, fourteen P. aeruginosa MDR (n=7), XDR (n=6) and PDR (n=1) isolates (inclusive of the two tap water isolates) were shortlisted for additional investigations. These isolates were characterized for antibiotic sensitivity, biofilm production, minimum biofilm inhibitory concentration (MBIC), presence of antibiotic resistance genes, efflux pumps, and integrons. Results : Mutations in gyr A, gyr B, par C, mex R, nfx B, mex B and mex F genes were correlated to enhanced antibiotic resistance. Notably, the isolates were also found to harbor integrons and blaNDM-1. Pulsed Field Gel Electrophoresis (PFGE) and Random Amplified Polymorphic DNA (RAPD) based phylogenetic analyses grouped the clinical isolates into three distinct ward specific clusters, while the tap water isolates were grouped into a separate cluster. Detection of nosocomial P. aeruginosa in the CTVS-ICU and urology ward triggered the activation of enhanced surveillance and infection control measures to contain and eliminate P. aeruginosa infections. Conclusions : Multiple clones of P. aeruginosa are prevalent in the study center. Hence, continuous screening and identification of potential reservoirs is absolutely essential to control the spread of drug resistant P. aeruginosa infections.

scholarly journals A Clinical-Bacteriological Profile of Multidrug Resistant Pseudomonas aeruginosa at Tertiary Care Hospital Shahjahanpur, Uttar Pradesh

2021 ◽  
Vol 10 (9) ◽  
pp. 301-313
Author(s):  
Jitendra Kumar Chaudhary
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Uttar Pradesh ◽  
Multidrug Resistant ◽  
Antimicrobial Treatment ◽  
Clinical Samples ◽  
Care Hospital ◽  
Bacteriological Profile ◽  
Drug Resistant Strains

The Pseudomonas aeruginosa is predominant agent causing nosocomial infections. In recent time, it develops resistance continuously to the antibiotics becomes Multidrug-resistant P. aeruginosa. So, in cystic fibrosis patents it difficult to eradicate P. aeruginosa infections with antimicrobial treatment. Therefore, focus on alternative mechanisms for treating P. aeruginosa infections. On the basis of growth, morphological and biochemical characteristics, P. aeruginosa strains were isolated from the clinical samples in this work. After that the antibiotic sensitivity was performed and the Multidrug resistant Pseudomonas aeruginosa was identified according to CLSI standard guideline chart by measuring the zone of inhibition for P. aeruginosa. The isolated strains showed resistance against three or more antibiotics, considered as MDR Pseudomonas aeruginosa. By antibiogram pattern 51 showed Multi drug resistant strains out of 102 isolated strains. As P. aeruginosa abide to develop resistance to the antibiotics, the quorum sensing increased transcriptional regulator QscR might performs another target. Thus the prevalence of MDR strains of Pseudomonas aeruginosa was investigated on current study. The antibiogram pattern revealed 51 MDR strains of P. aeruginosa. In which the majority of strains exhibited resistance towards Piperacillin (98%), Ciprofloxacin (90%), Ofloxacin (90%), Levofloxacin (80%) and Tobramycin (60%).

scholarly journals Antimicrobial drug sensitivity pattern of Pseudomonas aeruginosa in respiratory infections

2017 ◽  
Vol 6 (7) ◽  
pp. 1591
Author(s):  
Syed S. Ameen ◽  
Shanmukananda Prakash ◽  
Laxminarayana Bairy K. ◽  
Shahabuddin Soherwardi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Respiratory Infections ◽  
Tertiary Care ◽  
Empirical Therapy ◽  
Multidrug Resistant ◽  
Resistance Rate ◽  
Mortality And Morbidity ◽  
Antimicrobial Susceptibility Pattern ◽  
Sensitivity Pattern ◽  
Male Preponderance

Background: Pseudomonas aeruginosa, a gram-negative pathogen commonly associated with nosocomial infections is the most widespread multidrug-resistant pathogen causing pneumonia in hospitalized patients. Inadequate empirical therapy has been associated with high mortality and morbidity. Objective: To evaluate and analyze the antimicrobial susceptibility pattern of P. aeruginosa in respiratory infections in a tertiary care hospital.Methods: The study was carried out at Kasturba Hospital, Manipal from Jan 2011 to Dec 2011. Specimens of 63 in-patients were analyzed who were culture positive for P. aeruginosa.Results: Majority of patients were aged above 40yrs with a male preponderance. Specimens were taken from patients who were diagnosed with bronchiectasis, pneumonia, COPD, bronchial asthma etc. Overall the organism was most sensitive to carbapenems (87.3%) followed by cefoperazone-sulbactam combination (85.7%). Sensitivity to ceftazidime and cefepime was equal (82.5%) and was more when compared to piperacillin-tazobactam (81.5%). Overall resistance rate was highest for fluoroquinolones (23.8%) followed by aztreonam (22.2%).Conclusions: Hence we would like to recommend cefoperazone-sulbactam as the preferred antipseudomonal agent and carbapenems as reserved drugs in treating pseudomonal lung infections. Use of fluoroquinolones and aztreonam as monotherapy in resistant P. aeruginosa infections should be restricted.

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