Comparison of endothelial cell changes by specular microscopy between Ringer’s and Ringer’s lactate during phacoemulsification

2020 ◽  
Vol 1 (4) ◽  
pp. 218-223
Author(s):  
Eman Desoky ◽  
◽  
Hany Ahmed Helaly ◽  
Keyword(s):  
Endothelial Cell ◽  
Corneal Thickness ◽  
Cell Loss ◽  
Specular Microscopy ◽  
Ringer’S Solution ◽  
Age Related ◽  
Ringer’S Lactate ◽  
The Difference ◽  
Group 2 ◽  
Group 1

AIM: To compare the difference between using Ringer’s and Ringer’s lactate (RL) solutions as irrigating solutions during phacoemulsification and investigate their effect on corneal endothelium. METHODS: This was a prospective interventional double blinded clinical study that included 100 eyes of 100 patients aged between 50 to 65y suffering from a visually significant age-related cataract and scheduled for routine uncomplicated phacoemulsification. The included eyes were randomly divided into two equal groups (each group included 50 eyes). Group 1 received Ringer’s solution and group 2 received Ringer’s lactate as an irrigating solution. RESULTS: The first group with Ringer’s solution had a mean age of 57.5±8y and the second group with RL solution had a mean age of 58.6±9y. After 3mo, endothelial cell density decreased in group 1 by a mean of 8.5%, and in group 2 by a mean of 3.6% (P=0.013). Additionally, central corneal thickness increased in group 1 by a mean of 6.9% and in group 2 by a mean of 1.5% (P=0.006). By correlating the percentage of change in pachymetry and specular microscopic parameters with volume of irrigation solution used, there was no significant correlation. CONCLUSION: The use of Ringer’s lactate as an irrigating solution is associated with less endothelial cell loss postoperatively in comparison to Ringer’s solution and associated with decreased postoperative edema.

scholarly journals A Comparison of Endothelial Cell Loss in Combined Cataract and MIGS (Hydrus) Procedure to Phacoemulsification Alone: 6-Month Results

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Antonio M. Fea ◽  
Giulia Consolandi ◽  
Giulia Pignata ◽  
Paola Maria Loredana Cannizzo ◽  
Carlo Lavia ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cataract Surgery ◽  
Open Angle Glaucoma ◽  
Glaucoma Surgery ◽  
Cell Loss ◽  
Endothelial Cell Loss ◽  
Age Related ◽  
Before And After ◽  
Group 2 ◽  
Group 1

Purpose. To compare the corneal endothelial cell loss after phacoemulsification, alone or combined with microinvasive glaucoma surgery (MIGS), in nonglaucomatous versus primary open angle glaucoma (POAG) eyes affected by age-related cataract.Methods. 62 eyes of 62 patients were divided into group 1 (n=25, affected by age-related cataract) and group 2 (n=37, affected by age-related cataract and POAG). All patients underwent cataract surgery. Group 2 was divided into subgroups A (n=19, cataract surgery alone) and B (n=18, cataract surgery and MIGS). Prior to and 6 months after surgery the patients’ endothelium was studied. Main outcomes were CD (cell density), SD (standard deviation), CV (coefficient of variation), and 6A (hexagonality coefficient) variations after surgeries.Results. There were no significant differences among the groups concerning preoperative endothelial parameters. The differences in CD before and after surgery were significant in all groups: 9.1% in group 1, 17.24% in group 2A, and 11.71% in group 2B. All endothelial parameters did not significantly change after surgery.Conclusions. Phacoemulsification determined a loss of endothelial cells in all groups. After surgery the change in endothelial parameters after MIGS was comparable to the ones of patients who underwent cataract surgery alone.

scholarly journals Comparative study of anatomic and functional changes of the corneal endothelium in cataract patients undergoing phacoemulsification and MSICS

2021 ◽  
Vol 7 (2) ◽  
pp. 358-362
Author(s):  
Gurvinder Khosa ◽  
Karanijit Singh ◽  
Prempal Kaur ◽  
Rajesh Kumar ◽  
Upasna Ajmani
Keyword(s):  
Visual Acuity ◽  
Endothelial Cell ◽  
Central Corneal Thickness ◽  
Corneal Thickness ◽  
Cell Loss ◽  
P Value ◽  
Corrected Visual Acuity ◽  
Group 2 ◽  
Best Corrected Visual Acuity ◽  
Group 1

The purpose of this study was to compare the effect of Phacoemulsification and Manual small incision cataract surgery (MSICS) on the corneal endothelium and to assess its impact on visual acuity and induced astigmatism. In this prospective randomized study, 100 cases were randomly selected with the help of lottery system. 50 cases underwent phacoemulsification (group 1) and 50 underwent MSICS (group 2) by a single surgeon. The endothelial cell count, central corneal thickness, best corrected visual acuity and induced astigmatism was measured preoperatively and postoperatively on day 1, 7, 28 and on day 42. Mean endothelial cell loss (cells/mm2) in group 1 at the end of six weeks was 538.64 (20.59%) and in group 2 was 485.66 (19.20%) which was statistically insignificant (p >0.05). The mean central corneal thickness was reduced in both the groups with a mean of 0.51±0.04 mm in group 1 and 0.50±0.03 mm in group 2 which was statistically insignificant. Postoperative best corrected visual acuity of more than 6/18 was found in 49 (98%) cases in each group at six weeks, and was statistically insignificant (p value > 0.05). Phacoemulsification group had statistically significant less mean postoperative astigmatism than MSICS group (p value < 0.05). Both groups gave similar statistically insignificant results in relation to endothelial cell loss, central corneal thickness and best corrected visual acuity at the end of six weeks, although the mean astigmatism was more in group 2 in comparison to group 1 with a statistically significant difference.

scholarly journals Endothelial Cell Loss Rate Following Penetrating Keratoplasty: Optical versus Therapeutic Grafts

2021 ◽  
Author(s):  
Abdelrhman Shams ◽  
Ayman Gaafar ◽  
Rania Elkitkat ◽  
Mohamed Yousif
Keyword(s):  
Endothelial Cell ◽  
Graft Rejection ◽  
Penetrating Keratoplasty ◽  
Cell Loss ◽  
P Value ◽  
Specular Microscopy ◽  
Endothelial Cell Loss ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Abstract Purpose: To compare the rate of endothelial cell loss (ECL) following penetrating keratoplasty (PKP) for optical and therapeutic indications and to state whether therapeutic PKP is inferior to optical PKP or not.Methods: This is a Prospective, observational, comparative study that included patients who sought medical advice at the Cornea Outpatient Clinic of Ain Shams University Hospitals. The study enrolled two groups; group 1 included 30 corneas of 30 patients who performed optical PKP for various purposes, while group 2 comprised 30 corneas of 30 patients who were planned for performing therapeutic PKP for unhealed, resistant corneal infections. Specular microscopy was done to all the patients at the 3-, 6- and 12-months visits using Nidek CEM-530 (NIDEK Co., Ltd. Japan) specular microscope. Results: There were no statistically significant differences between both groups as regards to the timing of the graft clarity following surgery or the rate of ECL at the 3- and 6- months intervals, yet the rate of ECL was significantly higher in group 2 compared to group 1 at the 12 months interval (P-value <0.05), though the statistical difference was narrow from a clinical point of view. There was also no statistically significant difference between both groups regarding the rate of graft rejection.Conclusion: Therapeutic PKP can be considered non-inferior to optical PKP regarding the graft viability, the rate of ECL, and the rate of graft rejection along a follow up interval of one year.

scholarly journals Endothelial cell loss rate after penetrating keratoplasty: Optical versus therapeutic grafts

2021 ◽  
Vol 10 (2) ◽  
pp. 74-79
Author(s):  
Abdelrhman Shams ◽  
Ayman Abdelmoneim Gaafar ◽  
Rania Serag Elkitkat ◽  
Mohamed Omar Yousif
Keyword(s):  
Endothelial Cell ◽  
Graft Rejection ◽  
Penetrating Keratoplasty ◽  
Cell Loss ◽  
Specular Microscopy ◽  
Endothelial Cell Loss ◽  
Group 2 ◽  
Cell Loss Rate ◽  
Group 1

Background: This study aimed to compare the rate of endothelial cell loss (ECL) after penetrating keratoplasty (PKP) for optical versus therapeutic grafts at 3-, 6-, and 12-month postoperatively. Furthermore, the study aimed to investigate postoperative graft viability and the rate of graft rejection during the first year of follow-up for both indications. Methods: This was a prospective, observational, comparative study that included patients who sought medical advice at the cornea outpatient clinic of Ain Shams University Hospitals, Cairo, Egypt. The study recruited 60 patients: group 1 included 30 transplanted corneas of 30 patients who underwent optical PKP for various indications, while group 2 included 30 transplanted corneas of 30 patients who underwent therapeutic PKP for unhealed, resistant infectious keratitis. Specular microscopy was performed for all patients at the 3-, 6-, and 12-month follow-up visits using Nidek CEM-530 specular microscopy. Postoperative clinical examinations were performed at the same follow-up visits to detect graft rejection. Results: There were no statistically significant differences between the groups concerning the postoperative timing of graft clarity or the rate of ECL at 3- and 6-months postoperatively; however, the rate of ECL was significantly greater in group 2 than in group 1 at 12-months postoperatively (P = 0.03), although the difference was small from a clinical point of view. Moreover, there was no statistically significant difference between the groups in terms of the graft rejection rate. Conclusions: Therapeutic PKP results were comparable to optical PKP with respect to graft viability, the rate of ECL, and the rate of graft rejection 1 year after grafting. How to cite this article: Shams A, Abdelmoneim Gaafar A, Elkitkat RS, Omar Yousif M. Endothelial cell loss rate after penetrating keratoplasty: Optical versus therapeutic grafts. Med Hypothesis Discov Innov Ophthalmol. 2021 Summer; 10(2): 74-79. https://doi.org/10.51329/mehdiophthal1424

scholarly journals Analysis of the corneal endothelium in eyes of chickens using contact specular microscopy

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4199
Author(s):  
Luciane Albuquerque ◽  
João Antonio Tadeu Pigatto ◽  
Luciana Vicente da Rosa Pacicco Freitas
Keyword(s):  
Endothelial Cell ◽  
Corneal Endothelium ◽  
Single Layer ◽  
Specular Microscopy ◽  
Cell Area ◽  
Average Cell ◽  
Group 3 ◽  
Group 2 ◽  
Cellular Pleomorphism ◽  
Group 1

The corneal endothelium is a single layer of polygonal cells essential for corneal transparency. The objective of this study was to assess the parameters corneal endothelial cells in healthy chickens of different ages using a contact specular microscopy. A total 36 eyes of 18 chickens were evaluated in this study. After the humane slaughter of birds and subconjunctival enucleation, the eye bulbs were arranged into three groups according to the age of the chickens. Group 1 consisted of bulbs eyes of 6 chickens with ages ranging from 1 to 3 months old. Group 2 consisted of ocular bulbs 6 chickens with ages ranging from 5 to 12 months old. Group 3 consisted of bulbs eyes of 6 chickens with ages ranging from 24 to 40 months old. The contact specular microscopy was easy to perform and provided data such as endothelial cell density, average cell area and cellular pleomorphism. The results obtained with a contact specular microscopy revealed that the aging causes a decrease in the density of the corneal endothelium healthy chickens. Similarly, increasing the average endothelial cell area as well as the pleomorphism occurs. The present work suggests that the parameters of corneal endothelium of healthy chickens present change with advancing age.

scholarly journals ‘Comparison of Safety and Performance of Two Ophthalmic Viscosurgical Devices in Cataract Surgery’

2021 ◽  
Author(s):  
öznur işcan ◽  
Banu Torun Acar ◽  
Burcu Nurozler Tarakcı
Keyword(s):  
Endothelial Cell ◽  
Cataract Surgery ◽  
Corneal Thickness ◽  
Anterior Chamber Depth ◽  
Sodium Hyaluronate ◽  
Significant Difference ◽  
The Mean ◽  
Group 2 ◽  
And Performance ◽  
Group 1

Abstract Purpose: To compare the safety and performance of two ophthalmic viscosurgical devices (OVDs) Bio-Hyalur SV (Sodium Hyaluronate 3.0%) (Biotech Healthcare Group, Luzern, Switzerland) and Protectalon (sodium hyaluronate 2.0%) (VSY Biotechnology, Turkey) in cataract surgery. Methods: One hundred twenty eyes of one hundred twenty patients who underwent phacoemulsification surgery were included in the study. Postoperatively sixty eyes using Bio-Hyalur-SV were classified as Group 1, and sixty eyes using Protectalon as Group 2. Patients aged 45 and over, Grade I, II or III unilateral / double stained cataract, healthy eyes creating cataract inclueded in this study . Endothelial cell morphological parameters including endothelial cell density (ECD), cell number, cell area, coefficient of variation (CV) in cell size, cell hexagonality and central corneal thickness (CCT) were measured preoperatively and at postoperative first week, first and third month visits.Intraocular pressure (IOP) was measured with an applanation tonometer at every visit. Results: . There was a statistically significant decrease in the mean ECD all follow-up times when compared with the preoperative visit (p=0.000). In terms of mean ECD levels there was no significant difference between the two groups within three months postoperatively (p=0.616) In the first week after surgery, there was an significant increase in CCT in Group 1 and Group 2 respectively (p=0.000). The IOP was <23 mmHg in all of the patients on the first day after surgery. There was no significant difference in the incidence of IOP peaks between the two groups in every vizits. In both groups, a significant increase was observed in the mean IOP at first day, first week, and first month after surgery compared to preoperative values (p=0.000). But no significant difference in IOP increase in Group 1 (P=0.092), Group 2 (P=0.013) compared to preoperative values ​​at third month postoperatively (p <0.001 significant with Bonferrotti correction). Conclusion: The two OVD’s used in this study during cataract surgery were safe and effective. Both OVD’s resulted in similar rates of transient IOP increases and corneal endothelial damage also provided good anterior chamber depth and were fairly easy to remove.

scholarly journals Corneal Thickness and Endothelial Morphology in Normal Thai Eyes

2020 ◽  
Author(s):  
Napaporn Tananuvat ◽  
Natawan Khumchoo
Keyword(s):  
Endothelial Cell ◽  
Cell Density ◽  
Central Corneal Thickness ◽  
Corneal Thickness ◽  
Age Groups ◽  
Inverse Correlation ◽  
Cell Loss ◽  
Endothelial Cell Density ◽  
Specular Microscopy ◽  
The Mean

Abstract Background: This study aimed to determine the influence of age on central corneal thickness and corneal endothelial morphology as well as to identify the relationship between them in normal Thai eyes.Methods: Non-contact specular microscopy was performed in volunteers stratified into seven age groups ranging from 11-88 years. The corneal endothelial parameters studied included central corneal thickness (CCT), endothelial cell density (ECD), coefficient of variation in cell size (CV), cell area (CA) and percentage of regular hexagonal cells.Results: In a total of 501 subjects (1002 eyes), the mean age was 43.12 ± 18.80 years and 347(69.3%) were females. The mean CCT, ECD, CV, CA, and hexagonality was 533.80 ± 33.00 μm, 2,732±258 cell/mm2, 37.61 ± 6.76%, 369.04 ± 37.90 mm, and 49.03% ± 7.53%, respectively. There was a statistically significant inverse correlation between age and CCT (r = -0.212, P <0.001), ECD (r = -0.484, P <0.001), and hexagonality (r = -0.193, P <0.001). The estimate rate of endothelial cell loss was 0.2% per year, whereas CV and CA had statistically significant correlation with age (r = 0.200, P <0.001 and r = 0.475, P <0.001). CCT correlated directly with ECD (r = 0.177, P <0.001).Conclusion: Normative data for corneal endothelial morphology in healthy Thai eyes showed that ECD, CCT, and hexagonality were significantly decreased, while CV and CA were increased with aging. The central corneal thickness was correlated with the endothelial cell density.

scholarly journals Behavior and resistance in Simmental bull calves of different genotypes

2020 ◽  
Vol 193 (2) ◽  
pp. 54-61
Author(s):  
G. Levina ◽  
K. Tikhonov ◽  
O. A. Artemieva ◽  
M. V. Zelepukina
Keyword(s):  
Feeding Behavior ◽  
Feed Intake ◽  
Physical Contact ◽  
Age Related ◽  
Bull Calves ◽  
Intense Fear ◽  
The Difference ◽  
Group 2 ◽  
Herd Milk ◽  
Group 1

Abstract. The new genotypes of animals have been created with the use of the Simmental-seedstock herd improver bulls. Selective breeding to be effective for these animals requires understanding the genetic basis of the main types of behavior. For this purpose, an experiment with the Simmetal herd was performed. The average herd-milk production comprised 7.0 thousand kg milk per cow per year (Kursk Region). The bull calves were kept loose. They were divided into two groups of 6 animals each, representing the breeds formed of 1/4 Simmental × 3/4 Holstein in group 1 and 1/8 Simmental × 3/8 Holstein × 1/2 Montbeliarde in group 2. Age-related differences between the bull calves within the groups were no more than two weeks. It was ascertained that the progeny of the Montbeliarde bull fathers (group 2) tended to be calm, spending more time on social behavior. Thus, frequencies of allogrooming in these bull calves at 10–12 months old and 14–15 months old were 50 % and 33 % more, respectively, than that in their peers of a genotype formed of 1/4 Simmental × 3/4 Holstein. Feeding behavior activity and feed intake in the bull calves aged 10–12 months and 14–15 months from group 2 were 18.4 % and 10.8 % higher, respectively, than that in their peers from group 1. The bull calves from group 1 were more nervous, since head butting, which occurred with a physical contact, among the bull calves at 14–15 months old was observed in 50 % of them. In addition, frequencies of agitated behavior (which can be indicated by scratching) exhibited by the bull calves aged 10–11 months and 14–15 months were higher by 16 % and 34 %, respectively, than that in their peers produced from the Montbeliarde bulls. Intense fear associated with a change in the habitual place of keeping was exhibited by 33 % of bull calves from group 1. The innate resistance parameter values for the bull calves of both genotypes were corresponded to the normal variables. The difference between the 14–15-month-old bull calves in the serum bactericidal activity was in favor of the Montbeliarde bulls, comprising 0.1 μ g/mL. Therefore, using the Montbeliarde bulls, overall, has had a positive effect on behavior including feeding behavior activity and feed intake in the produced progeny. It contributed to increasing the liveweights in the bull calves at 10–12 months old and 14–15 months old by 68.5 kg and 97 kg, respectively, when compared to the bull calves with the high rates of consanguinity in Holsteins.

scholarly journals Analysis of the corneal endothelium in eyes of chickens using contact specular microscopy

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4199 ◽  
Author(s):  
Luciane Albuquerque ◽  
João Antonio Tadeu Pigatto ◽  
Luciana Vicente da Rosa Pacicco Freitas
Keyword(s):  
Endothelial Cell ◽  
Corneal Endothelium ◽  
Single Layer ◽  
Specular Microscopy ◽  
Cell Area ◽  
Average Cell ◽  
Group 3 ◽  
Group 2 ◽  
Cellular Pleomorphism ◽  
Group 1

The corneal endothelium is a single layer of polygonal cells essential for corneal transparency. The objective of this study was to assess the parameters corneal endothelial cells in healthy chickens of different ages using a contact specular microscopy. A total 36 eyes of 18 chickens were evaluated in this study. After the humane slaughter of birds and subconjunctival enucleation, the eye bulbs were arranged into three groups according to the age of the chickens. Group 1 consisted of bulbs eyes of 6 chickens with ages ranging from 1 to 3 months old. Group 2 consisted of ocular bulbs 6 chickens with ages ranging from 5 to 12 months old. Group 3 consisted of bulbs eyes of 6 chickens with ages ranging from 24 to 40 months old. The contact specular microscopy was easy to perform and provided data such as endothelial cell density, average cell area and cellular pleomorphism. The results obtained with a contact specular microscopy revealed that the aging causes a decrease in the density of the corneal endothelium healthy chickens. Similarly, increasing the average endothelial cell area as well as the pleomorphism occurs. The present work suggests that the parameters of corneal endothelium of healthy chickens present change with advancing age.

scholarly journals A survey of corneal changes caused by daily wear silicone hydrogel contact lenses

2015 ◽  
Vol 08 (06) ◽  
pp. 1550044 ◽  
Author(s):  
Beata Kettesy ◽  
Julianna Vardai ◽  
Andras Berta ◽  
Laszlo Modis ◽  
Adam Kemeny-Beke
Keyword(s):  
Endothelial Cell ◽  
Cell Density ◽  
Contact Lens ◽  
Contact Lenses ◽  
Corneal Thickness ◽  
Endothelial Cell Density ◽  
Silicone Hydrogel ◽  
Group 2 ◽  
One Year ◽  
Group 1

Purpose: The examination of the effects of second generation lotrafilcon B silicone hydrogel (SiH) lenses on the cornea when worn for three years of daily wear. Material and Methods: 55 healthy patients were divided into two groups: current hydrogel contact lens wearers refitted with lotrafilcon B lenses (Group 1; 28 patients) and neophyte contact lens wearers (Group 2; 27 patients). Each patient's subjective eye comfort was measured with a self-administered questionnaire. The corneas were analyzed using contact specular microscope to measure corneal thickness and the endothelium before the SiH lenses were fitted after four weeks, one month, six months, one year, two years, and three years of lens wear. Results: Subjective complaints of patients in Group 1 were reduced; however patients in Group 2 experienced discomfort during the first two to four weeks of use. In Group 1, objective examinations identified a decrease in endothelial cell density. In Group 2, the endothelial cell density increased slightly in the first two years but decreased after three years. The results indicate that lotrafilcon B slows down the deleterious effects of contact lenses. The coefficient of variation significantly decreased after six months in Group 1 (0.47 vs 0.44; p = 0.049), whereas, in Group 2, the hexagonal cells improved significantly after one month (27.78 vs 28.25; p = 0.025). Conclusion: Based on the subjects involved and the period of time under examination, it can be concluded that high-Dk SiH lenses support the physiological metabolism and functions of the cornea by improving oxygen provision.

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