scholarly journals INFLUENCE OF DEFICIENCY OF CALCIUM AND MAGNESIUM ON MAINTENANCE OF PEPTIDE CONNECTIONS IN HUMAN ERYTHROCYTES AT THE INCUBATION IN VITRO

2017 ◽  
Vol 17 (8) ◽  
pp. 179-182
Author(s):  
A.V. Isaeva ◽  
R.O. Klenov ◽  
N.A. Klenova
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Erythrocytes ◽  
Calcium And Magnesium ◽  
Presence And Absence

The maintenance of peptide connections inside red blood cells and in the incubatory environment in the conditions of presence and absence of ions of calcium and magnesium, action of 1,3-dimetilksantin and АТГ is studied. The increase in the maintenance of peptides inside erythrocytes in the conditions of blocking L-calcium of channels and АТГ and reduction of their maintenance in the incubatory environment which is not containing ions of calcium is revealed.

scholarly journals STATE SYSTEM OF HEMOGLOBIN AND THE ACTIVITY OF ENZYMES OF HUMAN ERYTHROCYTES UNDER CONDITIONS OF HYPERGLYCEMIA IN VITRO

2017 ◽  
Vol 21 (10) ◽  
pp. 174-180
Author(s):  
A.A. Panarina ◽  
A.A. Pestryakova ◽  
A.V. Sadychova ◽  
N.A. Klenova ◽  
E.A. Lebedeva
Keyword(s):  
Red Blood Cells ◽  
Membrane Permeability ◽  
Proteolytic Activity ◽  
Blood Cells ◽  
Glycosylated Hemoglobin ◽  
Hemoglobin Level ◽  
Human Erythrocytes ◽  
Glycosylated Hemoglobin Level ◽  
Activity Of Enzymes

The study of condition of the system of hemoglobin, release of peptide com- pounds, and proteolytic activity of human erythrocytes under conditions of hy- perglycemia of varying degrees is carried out. The increase in the level of hy- perglycemia is accompanied by a decrease of affinity of hemoglobin to oxygen and the growth of level of metgemoglobin, the glycosylated hemoglobin level is increased in conditions of severe hyperglycemia. The level of formation of pep- tides in erythrocytes and the level of output of their cells is determined by the increase in trypsin-like activity in cytosol and increased membrane permeability of red blood cells.

Oxidative damage to human red blood cells treated with chlorfenvinphos, an organophosphate insecticide (in vitro)

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Bożena Sosnowska ◽  
Bogumiła Huras ◽  
Hanna Nowacka-Krukowska ◽  
Bożena Bukowska
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Changes ◽  
Insect Pest ◽  
Human Erythrocytes ◽  
Human Organism ◽  
Organophosphate Insecticide ◽  
High Concentration

AbstractChlorfenvinphos (CFVF) is an organophosphorus insecticide, which was used to control insect pest on livestock and household pests such as flies, fleas, and mites. The molecular basis of toxic properties of CFVF in animals has been insufficiently studied. Blood can transport oxygen and nutrients as well as toxic compounds. Xenobiotics can enter to red blood cells and cause damage. Therefore, investigation of the toxicity of different compounds to erythrocytes is very important. The purpose of the present experiment was to evaluate the effect of this compound on human erythrocytes. We have evaluated the hemolysis, hemoglobin oxidation (met-Hb formation) and lipid peroxidation in human erythrocytes. Moreover, the changes in the level of reactive oxygen species (ROS) were assessed using flow cytometry as well as those in morphological changes of erythrocytes using phase contrast microscopy. This study describes the interaction of low concentrations of CFVF with human erythrocytes as well as the concentrations, which may enter human organism as a result of acute poisoning (0.5–250 μM). It was shown that CFVF only at high concentration induced changes in human erythrocytes. We have observed hemolysis (at 250 μM), changes in morphological parameters including echinocytes formation (at 250 μM), as well as increase in lipid peroxidation in erythrocytes (at 250 μM), ROS formation (at 100 μM) in red blood cells treated 1 hour with CFVF. Additionally, CFVF after 4 h of incubation oxidized hemoglobin, however, to a lower degree.

Evaluating Hemolytic and Photo Hemolytic Potential of Organophosphorus by In Vitro Method as an Alternative Tool Using Human Erythrocytes

2020 ◽  
Vol 20 (9) ◽  
pp. 738-745 ◽  
Author(s):  
Ana L.G. Soria ◽  
Fabiola R. Ramirez ◽  
Alberto B. Pliego ◽  
Héctor R.D. Guadarrama ◽  
Guadalupe P.M. Farrera ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Methyl Parathion ◽  
Sodium Dodecyl ◽  
Human Erythrocytes ◽  
The Other ◽  
In Vitro Assays ◽  
Haemolytic Activity ◽  
Other Hand

Aims: The present study aims to determine the phototoxic and haemolytic activity of organophosphorus. The use of alternative in vitro assays with human erythrocytes is suggested to predict the polluting effect of these products on health. Methodology: Human erythrocytes from Toluca Blood Bank were used. Sodium dodecyl sulfate was employed as a positive control. Additionally, the haemolysis percentage of three organophosphate (Acetate, Chlorpyrifos, Malathion, Methamidophos, Methyl Parathion) induced photo haemolysis formulated with surfactants on a concentration of 2 x 109 erythrocytes were evaluated. Finally, the products were classified as irritant or phototoxic. Results: Results showed that the HC50 red blood cells were similar for each organophosphate (Malathion and Methamidophos) indicating very irritant action with ratio classification (L/D) of 0.041 and 0.053, respectively. On the other hand, Chlorpyrifos was classified as an irritant with L/D= 0.14. On the other hand, the HC50 obtained photo hemolysis assays irradiated red blood cells was similar for each organophosphate (Acetate, Chlorpyrifos, Malathion, Methamidophos, Methyl Parathion) indicating no phototoxic action. Conclusion: As a conclusion, it can be said that the parameters of haemolysis and denaturation of proteins are good indicators to classify organophosphorus formulated with surfactants as irritating or phototoxic.

MOLECULAR EVENTS ASSOCIATED WITH VIPERA LATIFI VENOM EFFECT ON CONDITION OF HUMAN RED BLOOD CELLS

2016 ◽  
Vol 50 (2 (240)) ◽  
pp. 43-50
Author(s):  
L.A. Ghulikyan ◽  
M. Mohamadvarzi ◽  
G.V. Ghukasyan ◽  
A.V. Kishmairyan ◽  
N.A. Zaqaryan ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Erythrocytes ◽  
Human Red Blood Cells ◽  
Molecular Events ◽  
Membrane Structure And Function ◽  
Electrolyte Homeostasis ◽  
The Sixties ◽  
And Function

Viper venom as a hemolytic biochemical “cocktail” of toxins, primarily cause to the systemic alteration of blood cells. In the sixties and seventies, human erythrocytes were extensively studied, but the mechanical and chemical stresses commonly exerted on red blood cells continue to attract interest of scientists for the study of membrane structure and function. Here we monitor the effect of Vipera latifi venom on human erythrocytes ghost membranes using phase contrast microscopy and changes in ATPase activity under snake venom influence in vitro. The ion pumps (Na+, K+)-ATPase and (Ca2+, Mg2+)-ATPase plays a pivotal role in the active transport of certain cations and maintenance of intracellular electrolyte homeostasis. We also describe the action of Vipera latifi venom on the freeradical processes in the membrane of erythrocyte ghosts and changes of activity of superoxide dismutase in course of envenomation.

scholarly journals An In Vivo and In Vitro Model of Plasmodium falciparum Rosetting and Autoagglutination Mediated by varO, a Group A var Gene Encoding a Frequent Serotype

2008 ◽  
Vol 76 (12) ◽  
pp. 5565-5580 ◽  
Author(s):  
Inès Vigan-Womas ◽  
Micheline Guillotte ◽  
Cécile Le Scanf ◽  
Sébastien Igonet ◽  
Stéphane Petres ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Confidence Interval ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Model ◽  
Human Erythrocytes ◽  
Group A ◽  
Vitro Model

ABSTRACTIn theSaimiri sciureusmonkey, erythrocytes infected with the varO antigenic variant of thePlasmodium falciparumPalo Alto 89F5 clone bind uninfected red blood cells (rosetting), form autoagglutinates, and have a high multiplication rate, three phenotypic characteristics that are associated with severe malaria in human patients. We report here that varO parasites express avargene having the characteristics of group Avargenes, and we show that the varO Duffy binding-like 1α1(DBL1α1) domain is implicated in the rosetting of bothS. sciureusand human erythrocytes. The soluble varO N-terminal sequence (NTS)-DBL1α1recombinant domain, produced in a baculovirus-insect cell system, induced high titers of antibodies that reacted with varO-infected red blood cells and disrupted varO rosettes. varO parasites were culture adapted in vitro using human erythrocytes. They formed rosettes and autoagglutinates, and they had the same surface serotype and expressed the samevarOgene as the monkey-propagated parasites. To develop an in vitro model with highly homogeneous varO parasites, rosette purification was combined with positive selection by panning with a varO NTS-DBL1α1-specific mouse monoclonal antibody. The single-variant, clonal parasites were used to analyze seroprevalence for varO at the village level in a setting where malaria is holoendemic (Dielmo, Senegal). We found 93.6% (95% confidence interval, 89.7 to 96.4%) seroprevalence for varO surface-reacting antibodies and 86.7% (95% confidence interval, 82.8 to 91.6%) seroprevalence for the recombinant NTS-DBL1α1domain, and virtually all permanent residents had seroconverted by the age of 5 years. These data imply that the varO model is a relevant in vivo and in vitro model for rosetting and autoagglutination that can be used for rational development of vaccine candidates and therapeutic strategies aimed at preventing malaria pathology.

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

Effect of liposomal curcumin on red blood cells in vitro

2013 ◽  
Vol 1 (Suppl. 1) ◽  
pp. A4.1
Author(s):  
Angela Storka
Keyword(s):  
Red Blood Cells ◽  
Blood Cells

scholarly journals THE SYNTHESIS OF PROTOPORPHYRIN IN VITRO BY RED BLOOD CELLS OF THE DUCK

1950 ◽  
Vol 183 (2) ◽  
pp. 757-765 ◽  
Author(s):  
David Shemin ◽  
Irving M. London ◽  
D. Rittenberg
Keyword(s):  
Red Blood Cells ◽  
Blood Cells

Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan

2021 ◽  
pp. 153537022110132
Author(s):  
Shu-Qin Liu ◽  
Xiao-Ye Hou ◽  
Feng Zhao ◽  
Xiao-Ge Zhao
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cardiac Injury ◽  
Myocardial Regeneration ◽  
Matrix Metalloproteinase 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cardiac Tissues ◽  
Nucleated Red Blood Cells ◽  
Model Animal

Heart regeneration is negligible in humans and mammals but remarkable in some ectotherms. Humans and mammals lack nucleated red blood cells (NRBCs), while ectotherms have sufficient NRBCs. This study used Bufo gargarizan gargarizan, a Chinese toad subspecies, as a model animal to verify our hypothesis that NRBCs participate in myocardial regeneration. NRBC infiltration into myocardium was seen in the healthy toad hearts. Heart needle-injury was used as an enlarged model of physiological cardiomyocyte loss. It recovered quickly and scarlessly. NRBC infiltration increased during the recovery. Transwell assay was done to in vitro explore effects of myocardial injury on NRBCs. In the transwell system, NRBCs could infiltrate into cardiac pieces and could transdifferentiate toward cardiomyocytes. Heart apex cautery caused approximately 5% of the ventricle to be injured to varying degrees. In the mildly to moderately injured regions, NRBC infiltration increased and myocardial regeneration started soon after the inflammatory response; the severely damaged region underwent inflammation, scarring, and vascularity before NRBC infiltration and myocardial regeneration, and recovered scarlessly in four months. NRBCs were seen in the newly formed myocardium. Enzyme-linked immunosorbent assay and Western blotting showed that the levels of tumor necrosis factor-α, interleukin- 1β, 6, and11, cardiotrophin-1, vascular endothelial growth factor, erythropoietin, matrix metalloproteinase- 2 and 9 in the serum and/or cardiac tissues fluctuated in different patterns during the cardiac injury-regeneration. Cardiotrophin-1 could induce toad NRBC transdifferentiation toward cardiomyocytes in vitro. Taken together, the results suggest that the NRBC is a cell source for cardiomyocyte renewal/regeneration in the toad; cardiomyocyte loss triggers a series of biological processes, facilitating NRBC infiltration and transition to cardiomyocytes. This finding may guide a new direction for improving human myocardial regeneration.

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