scholarly journals Bactericidal effect of photodynamic therapy against methicillin-resistant Staphylococcus aureus strain with the use of various porphyrin photosensitizers.

2007 ◽  
Vol 54 (3) ◽  
pp. 665-670 ◽  
Author(s):  
Mariusz Grinholc ◽  
Bozena Szramka ◽  
Katarzyna Olender ◽  
Alfreda Graczyk
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bactericidal Effect ◽  
Aureus Strain ◽  
Methicillin Resistant ◽  
Essential Change ◽  
Log10 Unit ◽  
Porphyrin Photosensitizers ◽  
Unit Reduction

Photodynamic therapy (PDT) is based on photosensitizers activated by light of appropriate wavelength. Their activation leads to generation of singlet oxygen and free radicals responsible for the cytotoxic effect. The aim of this project was to compare the bactericidal effect of PDT using different porphyrin photosensitizers against a methicillin-resistant Staphylococcus aureus strain. Exogenous sensitizers (protoporphyrin IX and newly synthesized derivative, protoporphyrin diarginate) induced a 3 log10-unit reduction in bacterial viable counts. With the use of endogenous, ALA-induced porphyrins, a 1.6 log10-unit reduction was obtained. The sensitizers tested executed their antibacterial activity with no essential change in the antibiotic resistance pattern of the studied strain.

scholarly journals Photodynamic effect of protoporphyrin diarginate (PPArg2) on methicillin-resistant Staphylococcus aureus and human dermal fibroblasts.

2008 ◽  
Vol 55 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Mariusz Grinholc ◽  
Anna Kawiak ◽  
Julianna Kurlenda ◽  
Alfreda Graczyk ◽  
Krzysztof P Bielawski
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bactericidal Effect ◽  
Viable Count ◽  
Dermal Fibroblasts ◽  
Aureus Strain ◽  
Human Dermal Fibroblasts ◽  
Experimental Conditions ◽  
Methicillin Resistant ◽  
Unit Reduction

The worldwide rise in the antibiotic resistance of bacteria forces the development of alternative antimicrobial treatments. A potential approach is photodynamic inactivation (PDI). The aim of the present study was to determine the phototoxicity of protoporphyrin diarginate (PPArg(2)) against methicillin-resistant Staphylococcus aureus and human dermal fibroblasts. Different concentrations (0 to 20 microM) of PPArg(2) and light dose of 6 J cm(-2) were tested. Cell viability was evaluated using the methylthiazoletetrazolium (MTT) assay. Incubation with 10 microM followed by illumination yielded a 3.6 log(10)-unit reduction in the viable count for Staphylococcus aureus. At the same experimental conditions, only 22.5% of the fibroblasts were photoinactivated. Protoporphyrin diarginate at concentrations up to 20 microM demonstrated no toxicity towards S. aureus or fibroblasts when not irradiated. These results suggest that the protoporphyrin diarginate exerts a high bactericidal effect against methicillin-resistant S. aureus strain without harming eukaryotic cells.

scholarly journals Whole-Genome Sequence for Methicillin-Resistant Staphylococcus aureus Strain ATCC BAA-1680

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Luke T. Daum ◽  
Violet V. Bumah ◽  
Daniela S. Masson-Meyers ◽  
Manjeet Khubbar ◽  
John D. Rodriguez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Whole Genome Sequence ◽  
Aureus Strain ◽  
Whole Genome ◽  
Strain Atcc ◽  
Methicillin Resistant

Inhibition of a Methicillin-Resistant Staphylococcus aureus Strain in Afuega'l Pitu Cheese by the Nisin Z–Producing Strain Lactococcus lactis subsp. lactis IPLA 729

2004 ◽  
Vol 67 (5) ◽  
pp. 928-933 ◽  
Author(s):  
NATALIA RILLA ◽  
BEATRIZ MARTÍNEZ ◽  
ANA RODRÍGUEZ
Keyword(s):  
Staphylococcus Aureus ◽  
Lactococcus Lactis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Aureus Strain ◽  
Northern Spain ◽  
Potential Threat ◽  
Methicillin Resistant ◽  
Initial Inoculum ◽  
Lactococcus Lactis Subsp ◽  
Nisin Z

Methicillin-resistant Staphylococcus aureus strains are a potential threat for food safety because foodborne illness caused by methicillin-resistant Staphylococcus aureus has been reported even though these strains were only associated with nosocomial infections until recently. This article focuses on the inhibitory effect of the nisin Z–producing strain Lactococcus lactis subsp. lactis IPLA 729 on the growth of Staphylococcus aureus CECT 4013, a methicillin-resistant strain. S. aureus was inhibited by the presence of the nisin producer IPLA 729 in buffered Trypticase soy broth, milk, and Afuega'l Pitu cheese, an acid-coagulated cheese manufactured in Asturias, Northern Spain. A reduction of 3.66 log units was observed in Trypticase soy broth at the end of the incubation period. In milk, viable counts of S. aureus were undetectable or were reduced by 2.16 log units in 24 h depending on the initial inoculum (1.8 × 104 and 7.2 × 106 CFU/ml). The staphylococcal strain was also undetected in test cheeses in which the nisin Z producer was present whereas 2 log units were detected in control cheeses at the end of ripening.

scholarly journals A Novel Dicationic Boron Dipyrromethene-based Photosensitizer for Antimicrobial Photodynamic Therapy against Methicillin-Resistant Staphylococcus aureus

2020 ◽  
Vol 28 ◽  
Author(s):  
Priyanga Dharmaratne ◽  
Ligang Yu ◽  
Roy Chi-Hang Wong ◽  
Ben Chun-Lap Chan ◽  
Kit-Man Lau ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Skin Irritation ◽  
Scientific Basis ◽  
Positive Control ◽  
Methicillin Resistant ◽  
Boron Dipyrromethene ◽  
Mrsa Strains

Background: We report herein the synthesis of a novel dicationic boron dipyrromethene derivative (compound 3) which is symmetrically substituted with two trimethylammonium styryl groups. Methods: The antibacterial photodynamic activity of compound 3 was determined against sixteen methicillin-resistant Staphylococcus aureus (MRSA) strains, including four ATCC type strains (ATCC 43300, ATCC BAA-42, ATCC BAA-43, and ATCC BAA-44), two mutant strains [AAC(6’)-APH(2”) and RN4220/pUL5054], and ten non-duplicate clinical strains of hospital- and communityassociated MRSA. Upon light irradiation, the minimum bactericidal concentrations of compound 3 were in the range of 1.56-50 µM against all the sixteen MRSA strains. Interestingly, compound 3 was not only more active than an analogue in which the ammonium groups are not directly connected to the pconjugated system (compound 4), but also showed significantly higher (p < 0.05) antibacterial potency than the clinically approved photosensitizer methylene blue. The skin irritation of compound 3 during topical application was tested on human 3-D skin constructs and proven to be non-irritant in vivo at concentrations below 1.250 mM. In the murine MRSA infected wound study, the colony forming unit reduction of compound 3 + PDT group showed significantly (p < 0.05) higher value (>2.5 log10) compared to other test groups except for the positive control. Conclusion: In conclusion, the present study provides a scientific basis for future development of compound 3 as a potent photosensitizer for photodynamic therapy for MRSA wound infection.

scholarly journals Complete Genome Sequence of Hospital-Acquired Methicillin-Resistant Staphylococcus aureus Strain WCUH29

2019 ◽  
Vol 8 (23) ◽  
Author(s):  
Ting Lei ◽  
Ying Zhang ◽  
Junshu Yang ◽  
Kevin Silverstein ◽  
Yinduo Ji
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Model System ◽  
Aureus Strain ◽  
Methicillin Resistant ◽  
Content Type ◽  
Hospital Acquired

The hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA) strain WCUH29 has been intensively and widely used as a model system for identification and evaluation of novel antibacterial targets and pathogenicity. In this announcement, we report the complete genome sequence of HA-MRSA WCUH29 (NCIMB 40771).

scholarly journals Effect of exogenous glycine on peptidoglycan composition and resistance in a methicillin-resistant Staphylococcus aureus strain.

1996 ◽  
Vol 40 (6) ◽  
pp. 1498-1503 ◽  
Author(s):  
B L de Jonge ◽  
Y S Chang ◽  
N Xu ◽  
D Gage
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Aureus Strain ◽  
Methicillin Resistant ◽  
Resistance Phenotype ◽  
Concomitant Decrease

A highly homogeneously methicillin-resistant Staphylococcus aureus strain was grown in the presence of various concentrations of exogenous glycine. Increasing concentrations of glycine in the medium resulted in a decrease in methicillin resistance and the appearance of a heterogeneous resistance phenotype. Parallel to the gradual changes in resistance was an alteration in the muropeptide composition of peptidoglycan. Increasing concentrations of glycine in the medium resulted in peptidoglycan in which muropeptides with a D-alanyl-D-alanine terminus were replaced with D-alanyl-glycine-terminating muropeptides. The disappearance of D-alanyl-D-alanine-terminating muropeptides in peptidoglycan and the concomitant decrease in resistance indicate a central role for D-alanyl-D-alanine-terminating precursors in methicillin resistance.

scholarly journals Characterization of a Catalase-Negative Methicillin-Resistant Staphylococcus aureus Strain

2007 ◽  
Vol 45 (8) ◽  
pp. 2684-2685 ◽  
Author(s):  
B. M. Gruner ◽  
S.-R. Han ◽  
H.-G. Meyer ◽  
U. Wulf ◽  
S. Bhakdi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Aureus Strain ◽  
Methicillin Resistant
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