scholarly journals A STUDY ON SEROPREVALENCE OF DENGUE IGM ANTIBODY IN SUSPECTED CASE OF DENGUE FEVER IN AND AROUND JAMNAGAR, GUJARAT (INDIA)

2017 ◽  
Vol 4 (94) ◽  
pp. 5883-5886
Author(s):  
Saurabh Chhotalal Gamit ◽  
Hitesh Kantilal Shingala ◽  
Mala Sinha
Keyword(s):  
Dengue Fever ◽  
Suspected Case ◽  
Igm Antibody

scholarly journals Evaluation of Six Commercial Point-of-Care Tests for Diagnosis of Acute Dengue Infections: the Need for Combining NS1 Antigen and IgM/IgG Antibody Detection To Achieve Acceptable Levels of Accuracy

2011 ◽  
Vol 18 (12) ◽  
pp. 2095-2101 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Mark S. Bailey ◽  
Ampai Tanganuchitcharnchai ◽  
Kemajittra Jenjaroen ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Sensitivity And Specificity ◽  
Dengue Infection ◽  
Antibody Test ◽  
Antibody Detection ◽  
Sample Collection ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
Antibody Tests

ABSTRACTSix assays were evaluated in this study to determine their suitability for the diagnosis of acute dengue infection using samples from 259 Sri Lankan patients with acute fevers (99 confirmed dengue cases and 160 patients with other confirmed acute febrile illnesses): (i) the Merlin dengue fever IgG & IgM combo device (Merlin), (ii) the Standard Diagnostics Dengue Duo nonstructural 1 (NS1) antigen and IgG/IgM combo device (Standard Diagnostics, South Korea), (iii) the Biosynex Immunoquick dengue fever IgG and IgM (Biosynex, France) assay, (iv) the Bio-Rad NS1 antigen strip (Bio-Rad, France), (v) the Panbio Dengue Duo IgG/IgM Cassette (Inverness, Australia), and (vi) the Panbio dengue NS1 antigen strip (Inverness, Australia). The median number of days of fever prior to admission sample collection was 5 days (interquartile range, 3 to 7 days). Sensitivity and specificity of the NS1 antigen tests ranged from 49 to 59% and from 93 to 99%, respectively, and sensitivity and sensitivity of the IgM antibody test ranged from 71 to 80% and from 46 to 90%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics Dengue Duo test gave the best compromise of sensitivity and specificity (93% and 89%, respectively) and provided the best sensitivity in patients presenting at different times after fever onset. The Merlin IgM/IgG antibody tests correctly classified 64% and 86% of the primary and secondary dengue infection cases, respectively, and the Standard Diagnostics IgM/IgG antibody tests correctly classified 71% and 83% of the primary and secondary dengue infection cases, respectively. This study provides strong evidence of the value of combining dengue antigen- and antibody-based test results in the rapid diagnostic test (RDT) format for the acute diagnosis of dengue.

scholarly journals Sera-epidemiology of Dengue Virus Infection in Clinically Suspected Patients Attended in Dhaka Medical College Hospital During January to December 2016

2018 ◽  
Vol 26 (2) ◽  
pp. 111-116
Author(s):  
Monira Pervin ◽  
Afroza Akbar Sweety ◽  
Mohammad Zaid Hossain ◽  
Rabeya Sharmin ◽  
Nusrat Fatema ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Clinical Manifestations ◽  
Medical College Hospital ◽  
Dengue Virus Infection ◽  
College Hospital ◽  
Medical College ◽  
Igm Antibody ◽  
Orbital Pain

Introduction: Dengue is now established as one of the most important Arboviral infection in tropical countries. As the epidemic continues worldwide, this Aedes mosquito-transmitted pathogen is considered a major re-emerging tropical disease and significant public health concern. The study was conducted to determine the sero-epidemiology and clinical picture of dengue virus infection among the clinically suspected patients attended in Dhaka Medical College Hospital during January to December 2016.Materials and Methods: The study was carried out on 145 clinically suspected Dengue patients attended in Dhaka Medical College Hospital from January to December 2016 who had fever with temperature >39°C. Blood sample was collected during acute febrile phase of patients and separated sera were tested for NS1 dengue antigen and IgM antibodies using commercial test kits (NS1 by OMC Healthcare (Pvt.) Ltd & IgM antibody by Omega Diagnostics Ltd.).Results: Of the total 145 patients 40 (27.6%) were positive for Dengue NS1 antigen and or IgM antibody test. Of the positive 40 patients, 18 (45%) were positive for NS1, 19 (47.5%) were positive for IgM and 3 (7.5%) were positive for both NS1 and IgM antibody. Major clinical manifestations were fever with arthralgia (79.5%) and retro-orbital pain (50%).Conclusion: Outbreak of Dengue fever is continuing every year and typical presentation of Dengue fever has changed. Arthralgia and retro orbital pain was the most common clinical feature in the present study instead of headache and rash with fever. A large number of suspected dengue cases were negative by Dengue tests for antigen NS1 and IgM antibody which suggests other organisms of similar clinical manifestations are becoming prevalent in Dhaka city.J Dhaka Medical College, Vol. 26, No.2, October, 2017, Page 111-116

scholarly journals Evaluation of NS1, IgM ELISA and RT-PCR in diagnosis of dengue fever

2018 ◽  
Vol 6 (7) ◽  
pp. 2440
Author(s):  
Deepak Kumar ◽  
Rajesh Kumar Verma ◽  
Amit Singh ◽  
Manoj Kumar ◽  
Dharmendra Prasad Singh ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Dengue Fever ◽  
Sensitivity And Specificity ◽  
Late Phase ◽  
Accurate Diagnosis ◽  
Suspected Case ◽  
Rt Pcr ◽  
Igm Elisa ◽  
Antigen Elisa ◽  
Ns1 Antigen

Background: Dengue fever is a mosquito borne disease caused by flavivirus. Its cases are increasing in India with increasing mortality rate year by year hence, prompt and accurate diagnosis is necessary to prevent morbidity and mortality.Methods: In this study we enrolled 125 clinically suspected cases of dengue. All the collected samples were processed for RT-PCR, NS1 and IgM ELISA. We evaluated NS1 antigen ELISA alone, and combination of NS1 and IgM ELISA against RT-PCR.Results: Among 125 clinically suspected case 67 were positive by RT-PCR and 58 were negative. Sensitivity and Specificity of NS1 ELISA and NS1 with IgM ELISA (in combination) against RT-PCR were 83.58%, 94.82% and 95.55%, 79.31% respectively. (p<0.001).Conclusions: The NS1 ELISA alone was sufficient to detect acute phase of dengue fever, although, combination of NS1 and IgM proved to be most appropriate method for detection of acute as well as late phase of dengue fever.

scholarly journals THE STUDY OF DETECTION OF DENGUE CASES BY NS1 ANTIGEN AND IGM ANTIBODY IN RIMS, ADILABAD, INDIA.

2019 ◽  
Vol 3 (11) ◽  
Author(s):  
Dr. Tanajee Zade ◽  
Dr. K. Srinivas ◽  
Dr. Akshay Berad
Keyword(s):  
Early Diagnosis ◽  
Dengue Fever ◽  
Dengue Infection ◽  
Tertiary Care ◽  
High Morbidity ◽  
Care Hospital ◽  
Serum Samples ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen

Dengue fever is an acute febrile arboviral disease affecting tropical & subtropical regions of the world. Dengue infection produces a spectrum of clinical illness, ranging from an asymptomatic to its most severe form like dengue haemorrhagic fever and dengue shock syndrome. In view of high morbidity and mortality, it is imperative to have a rapid and sensitive laboratory assay for early detection of the dengue infection. The newer parameter NS1 antigen has gained a lot of interest for early diagnosis of the disease. Detection of non-structural antigen (NS1 Ag), IgM and IgG antibody may help in the early diagnosis. The present study was conducted in a RIMS Adilabad, tertiary care hospital & medical college in the Department of  General Medicine. A total of 100 serum samples were processed from suspected cases of dengue fever by using dengue test for detection of NS1 antigen and IgG antibodies. Platelet counts of all these cases were noted. . Of these 100 subjects 85 were serologically proved to have dengue illness, 57 patients were NS1 antigen positive, 28 patients were IgM antibody positive patients. As the NS1 antigen is detectable in blood from day one after onset of fever, its assay is an effective tool for early diagnosis of dengue infection so as to avoid complications. Key words:  Dengue, NS1 Antigen, IgM antibody, Platelet

scholarly journals Clinical Profile and Laboratory Findings of Dengue Fever in Children

2021 ◽  
Vol 11 (5) ◽  
pp. 30-37
Author(s):  
Mahesh Ahirrao ◽  
Ashish Patil ◽  
Anjali Kurup
Keyword(s):  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Clinical Profile ◽  
Health Concern ◽  
Age Group ◽  
Laboratory Findings ◽  
Igm Antibody ◽  
Ns1 Antigen

Background: Dengue is an arthropod borne viral hemorrhagic fever. It is a major public health concern throughout the tropical and subtropical regions of the world. Objectives: To study clinical profile and laboratory findings of dengue infection in children. Materials and Methods: Children up to the age of 15 years who were confirmed serologically dengue positive (either positive NS1 antigen or positive IgM antibody by ELISA method were included in study. Total 100 patients were studied. Pre tested, semi structured questionnaire were developed and used for data collection. Result and Discussion: In current study, maximum patients were found in Dengue Hemorrhagic Fever (DHF) group (68%), followed by Dengue Fever (25%). Fever was most common clinical manifestation. Maximum patients in this study were in 10 to 15 years of age group, male being more commonly affected. Thrombocytopenia and leukopenia were present in 90% and 69% of patients respectively. Conclusion: School going children were more exposed to mosquito bites during day time school activities, thus maximum patients were from 10 to 15 years of age group. In early diagnosis of dengue fever, ELISA NS1 antigen is seen more promisable. When used in combination with ELISA IgM antibody, it significantly improves the diagnostic algorithm. Thrombocytopenia is more consistent feature of Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). Key words: Dengue Hemorrhagic Fever (DHF), Dengue Shock Syndrome (DSS), ELISA NS1 antigen, ELISA IgM antibody.

scholarly journals Increasing number of secondary dengue cases: a concern

2019 ◽  
Vol 7 (5) ◽  
pp. 1558
Author(s):  
Kumar S. Abhishek ◽  
Oves Siddiqui ◽  
Anita Chakravarti
Keyword(s):  
Dengue Fever ◽  
Fatal Outcome ◽  
Severe Dengue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igg Antibody ◽  
Past Infection ◽  
Igm Antibody ◽  
Igg Avidity ◽  
Increasing Trend

ackground: Dengue fever is caused by mosquito borne arbovirus of family Flaviviridae, Aedes agypti as the principle vector. In the recent past Delhi has witnessed several outbreaks affecting thousands of individuals and many of them get re-infected during subsequent years forming a bulk of secondary dengue cases putting them at risk of developing severe dengue.Methods: A total of 150 serum samples from suspected dengue cases were tested for dengue fever by NS-1 antigen and IgM antibody enzyme-linked immunosorbent assay (ELISA) followed by categorization into primary and secondary dengue using IgG avidity ELISA.Results: Out of total 150 clinically suspected dengue cases, 56 were positive either by Dengue NS-1 antigen or dengue IgM antibody or both. On the basis of dengue IgG avidity ELISA among 56 diagnosed dengue cases, 30 (53.57%) were found to be of secondary dengue.Conclusions: There is increasing trend of dengue cases in Delhi since past one decade. Being hyper-endemic area for dengue, more than 25% population have been reported to have past infection of dengue. Due to increased prevalence and simultaneous circulation of more than one serotypes, number of secondary dengue cases is also increasing. Since majority of severe dengue cases are associated with secondary dengue, early diagnosis and treatment can significantly reduce the fatal outcome. Thus, avidity testing for IgG antibody becomes an important tool.

Dengue Fever Reemerges in Florida After 75 Years

2010 ◽  
Vol 44 (8) ◽  
pp. 19
Author(s):  
ROXANNA GUILFORD-BLAKE
Keyword(s):  
Dengue Fever
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