Evaluation of Cell Block Diagnostics in Intra-Abdominal Lesions with Radiological and Clinical Correlation in a Tertiary Centre in North Karnataka Region

2021 ◽  
Vol 8 (19) ◽  
pp. 1397-1402
Author(s):  
Md Hamed Altaf Mali ◽  
Anita A. M ◽  
Meenakshi Meenakshi ◽  
Anuradha G Patil
Keyword(s):  
Diagnostic Yield ◽  
Cost Effective ◽  
Needle Aspiration ◽  
Vital Role ◽  
Cell Block ◽  
Diagnostic Modality ◽  
Fluid Analysis ◽  
Neoplastic Lesions ◽  
Malignant Lesions ◽  
Abdominal Lesions

BACKGROUND Cytological examination of intra-abdominal lesions is crucial in diagnosis, staging, and prognosis of suspected malignancies. Fine needle aspiration cytology (FNAC) is a routine diagnostic modality for diagnosis. FNAC is a reliable, rapid, cost effective procedure and plays an important role particularly in low resource centres. FNAC along with cell block has shown remarkable result in diagnosis of malignant lesions and its sub-classification using various antibody markers. METHODS A total of 102 cases of intra-abdominal lesions were studied over a period of one year in this descriptive study. Ultrasound sonography (USG) guided FNACs were done in 89 cases and ascetic / peritoneal fluid analysis in 13 cases. Cell blocks were made in 95 cases and immunohistochemistry (IHCs) were done in 69 cases of suspected / malignant intra-abdominal lesions to confirm the diagnosis. RESULTS Out of 102 cases, cytological smears in 89, fluid smears in 13 and cell blocks in 95 cases were available respectively. Most common intra-abdominal organ involved was liver (21.56 %) followed by retroperitoneum (17.65 %) and lymph nodes (14.70 %). The intra-abdominal lesions were classified into non - neoplastic (16.67 %) and neoplastic lesions (83.33 %). Neoplastic lesions (83.33 %) were further sub classified into benign (15.68 %) and malignant (67.65 %). IHC was done in all malignant lesions for confirmation of primary and / or metastasis. Complete concordance between cell blocks and smears was observed in 95 / 102 cases (93.14 %), while discordances were noted in 05 out of 102 cases (06.86 %). CONCLUSIONS Cell blocks play a vital role in cytopathology and as an adjunct to the routine cytology smears and body fluids. Cell buttons are cost effective and simple to make without involving much higher technicality. Cell block sections offer advantages with respect to cellular architecture, archival storage and application of IHCs which help in pin-pointing the diagnosis. Hence, cell blocks must be considered to increase the diagnostic yield and efficacy. KEYWORDS Cytopathology, Malignant Lesions, Immuno - Histochemistry, Diagnostic Utility

Yield and Complications in Ultrasound-Guided Biopsy of Abdominal Lesions

1995 ◽  
Vol 36 (4-6) ◽  
pp. 485-490 ◽  
Author(s):  
R. S. Nyman ◽  
J. Cappelen-Smith ◽  
J. Brismar ◽  
W. von Sinner ◽  
I. Kagevi
Keyword(s):  
Diagnostic Yield ◽  
Correct Diagnosis ◽  
Needle Aspiration ◽  
Benign Disease ◽  
Bleeding Complications ◽  
Benign Lesions ◽  
Ultrasound Guided ◽  
Focal Lesions ◽  
Malignant Lesions ◽  
Abdominal Lesions

A series of 458 consecutive ultrasound-guided biopsies in 347 patients − 171 fine-needle aspiration biopsies (FNABs) and 287 1.2-mm needle core biopsies (NCBs) − was analysed for diagnostic yield and complications. FNAB was diagnostic in 107 (64%) biopsies of focal lesions with a correct diagnosis of malignancy in 86 of 125 biopsies (69%) and of benign disease in 21 of 43 (49%) biopsies. NCB provided a correct diagnosis in 189 (90%) biopsies for focal lesions, divided into 140 of 159 (88%) correct for malignancy and 49 of 50 (98%) correct for benign disease. In 69 patients examined with both FNAB and NCB on the same occasion, 50 out of 55 malignant lesions were identified with NCB but only 34 with FNAB; all 14 benign lesions were correctly identified by NCB, and only 6 by FNAB. Clinical relevant bleeding complications occurred in 6 out of 458 biopsies (1.3%) − 3 out of 287 following NCB (1.0%) and 3 out of 171 following FNAB (1.8%). It is concluded that if FNAB is replaced with 1.2-mm NCB using an automated biopsy gun, the diagnostic accuracy for abdominal lesions increases significantly (p<0.001), while the complication rate remains the same.

EVALUATION OF CELL BLOCK TECHNIQUE VERSUS CONVENTIONAL CYTOLOGY ON BRONCHOSCOPY GUIDED NEEDLE ASPIRATION/ BRUSH CYTOLOGY FOR DIAGNOSIS OF LUNG CANCER

2021 ◽  
pp. 64-66
Author(s):  
Manveer kour Raina ◽  
Neena Gupta ◽  
Sanjeev Kumar ◽  
Anuradha Kusum
Keyword(s):  
Gold Standard ◽  
Diagnostic Yield ◽  
Needle Aspiration ◽  
Cell Block ◽  
Brush Cytology ◽  
Conventional Cytology ◽  
Cytological Preparation ◽  
True Conclusion ◽  
Diagnosis Of Lung Cancer ◽  
Cytology Study

INTRODUCTION: Bronchoscopy is a safe and effective method for diagnosing lung carcinomas with a variation in the diagnostic yield with different bronchoscopy guided procedures. Cell block technique has shown an addition cases positivity in diagnosing carcinomas as compared to the conventional method. AIM: The present study was aimed to evaluate the diagnostic utility of cell block technique on Bronchoscopy guided needle aspiration/ Brush and also to compare cytological preparation with cell block. MATERIAL AND METHODS: A total of 50 cases were included in the study that was suspected to be having lung carcinoma. These patients went under bronchoscope guided aspirations (TBNA, EBNA, and Brush). Smears were immediately made for conventional cytology study and well as in another aliquot samples were collected to prepare cell blocks following which H&E staining was done. RESULTS: Out of 50 cases, 8 cases came out to be negative on conventional smears and when compared with cell block technique 4 additional cases came out to be positive who were negative on conventional smears. The diagnosis were compared with histopathology biopsies keeping it as a gold standard and results on cell block techniques were conrmed to be true. CONCLUSION: Out of 50 cases, an additional 4 more cases were diagnosed malignant by using the cell blocks technique but there were few drawbacks with cell block technique. In few of the cases on cell block, cellularity was very less, cells morphology was also not very clear and some showed cells entrapped in a clusters. The conclusion made out of this study is that cell block technique is more accurate than the cytological smears and when used in combination diagnostic efcacy will be improved.

scholarly journals Pull vs. wet: rendimiento diagnóstico y calidad de las muestras de las PAAF guiadas por USE en las masas sólidas del páncreas

2021 ◽  
Vol 51 (1) ◽  
Author(s):  
Cecilia Curvale ◽  
Ignacio Málaga ◽  
Paloma Rojas Saunero ◽  
Viviana Tassi ◽  
Enrique Martins ◽  
...  
Keyword(s):  
Endoscopic Ultrasound ◽  
Fine Needle Aspiration ◽  
Diagnostic Yield ◽  
Needle Aspiration ◽  
Double Blind Study ◽  
Cell Block ◽  
Ultrasound Guided ◽  
Double Blind ◽  
Fine Needle ◽  
Significant Difference

Differential diagnosis of pancreatic masses is challenging. The endoscopic ultrasound-guided fine-needle aspiration method with the highest diagnostic yield has not been established. It was realized a prospective, randomized, double-blind study of the endoscopic ultrasound-guided fine-needle aspiration in solid lesions of the pancreas to compare and evaluate diagnostic yield and aspirate quality between wet and pull technique. Forty-one patients were enrolled. The wet technique presented a sensitivity, a specificity, a positive and negative predictive value, and a diagnostic accuracy of 58.3%, 100%, 100%, 25% and 63.4%, respectively. In the capillary technique they were: 75%, 100%, 100%, 35.7% and 78.1%, respectively. Comparing the diagnostic yield between both techniques, there was no statistically significant difference (McNemar’s test p = 0.388). Regarding the cellularity of the specimen, both in cytology and the cell block samples, no significant difference was observed between the techniques (p = 0.84 and 0.61, respectively). With respect to contaminating blood in the specimen, there was no difference in cytology samples (p = 0.89) and no difference in cell block samples (p = 0.08). The suitability of cytology samples for diagnosis was similar in both techniques (wet = 57.5% and capillary = 56.7%, p = 0.94) and there was no difference in cell block samples (wet = 75% and capillary = 66.1%, p = 0.38). In this study we did not observe differences in diagnostic yield or sample quality. Since both techniques are effective, we suggest the simultaneous and alternate use of both methods.

scholarly journals Molecular Biologic Approach to the Diagnosis of Pancreatic Carcinoma Using Specimens Obtained by EUS-Guided Fine Needle Aspiration

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Kiyohito Kato ◽  
Hideki Kamada ◽  
Takayuki Fujimori ◽  
Yuuichi Aritomo ◽  
Masahiro Ono ◽  
...  
Keyword(s):  
Pancreatic Carcinoma ◽  
Fine Needle Aspiration ◽  
Early Stage ◽  
Cost Effective ◽  
Needle Aspiration ◽  
Diagnostic Techniques ◽  
Pancreatic Tumors ◽  
Diagnostic Modality ◽  
Fine Needle ◽  
Response To Chemotherapy

We review the utility of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA), a rapid, safe, cost-effective, and accurate diagnostic modality for evaluating pancreatic tumors. EUS-FNA is currently used for the diagnosis and staging of pancreatic tumors. The sensitivity of EUS-FNA for pancreatic malignancy ranges from 75% to 94%, and its specificity approaches 100% in most studies. However, EUS-FNA has some limitations in the diagnosis of well-differentiated or early-stage cancers. Recent evidence suggests that molecular biological analysis using specimens obtained by EUS-FNA improves diagnostic sensitivity and specificity, especially in borderline cytological cases. It was also reported that additional information regarding patient response to chemotherapy, surgical resectability, time to metastasis, and overall survival was acquired from the genetic analysis of specimens obtained by EUS-FNA. Other studies have revealed that the analysis of KRAS, MUC, p53, p16, S100P, SMAD4, and microRNAs is helpful in making the diagnosis of pancreatic carcinoma. In this paper, we describe the present state of genetic diagnostic techniques for use with EUS-FNA samples in pancreatic diseases. We also discuss the role of molecular biological analyses for the diagnosis of pancreatic carcinoma.

scholarly journals Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting

2020 ◽  
Vol 5 (6) ◽  
pp. 167
Author(s):  
Hiroshani Hansamali Kulatunga ◽  
Ajith Aloka Pathirana ◽  
Neluka Fernando ◽  
Bawantha Gamage ◽  
Apsara Epa ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Cost Effective ◽  
Needle Aspiration ◽  
Resource Poor Setting ◽  
Cell Block ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Cost Effective Method ◽  
Resource Poor ◽  
Needle Aspiration Cytology

scholarly journals FNAC and histopathological correlation of salivary gland lesions: an observational study

2017 ◽  
Vol 4 (7) ◽  
pp. 2148 ◽  
Author(s):  
Sneha Kakoty ◽  
Tridip Dutta Baruah ◽  
C. P. Ganesh Babu
Keyword(s):  
Salivary Gland ◽  
Observational Study ◽  
Needle Aspiration ◽  
Age Group ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Histopathological Correlation ◽  
Neoplastic Lesions ◽  
Malignant Lesions ◽  
Needle Aspiration Cytology

Background: Fine needle aspiration cytology (FNAC) is a sensitive and specific and yet an economically effective technique for diagnosis of salivary gland lesions. FNAC of salivary glands has achieved a pivotal role in the diagnosis and management of salivary gland lesions since its induction by Stewart et al in 1933. However, it has always been under scrutiny when compared to histopathology. Histopathology of salivary gland lesions is still the final method to establish diagnosis and predicting prognosis in these lesions.1Methods: A prospective observational study of 50 patients with salivary gland lesions was done at Gauhati Medical College and hospital from June 2011 to May 2012. 39 patients underwent histopathological confirmation. Cases with histopathological correlation were included in calculating diagnostic accuracy. The cytological findings were correlated with that of the histopathological diagnosis to obtain the accuracy of the cytological diagnosis. The parameters of diagnostic validity of the cytological technique in terms of sensitivity, specificity and predictive value were evaluated.Results: Study population included patients of age group ranging from 13-70 years with median age group being 31-40 years (30%). 54% of the affected patients were male with the parotid being the most commonly involved gland (62%). Neoplastic lesions constituted the major bulk of the lesions (39 cases, 78%) with benign tumours constituting 54%. The most commonly involved benign neoplastic lesion was pleomorphic adenoma (22cases, 44%). Among the non-neoplastic lesions (22%), the acute sialadenitis was frequently noted. Histopathological correlations were available in 39 cases with 11 cases being the malignant lesions. The acute sialadenitis lesions did not undergo histological examination. 28 cases of non-malignant lesions underwent the histological confirmation. In the present study, the specificity and the sensitivity were found to be 96.42% and 90.91% respectively. The predictive value of salivary gland cytology was 90.91% and diagnostic accuracy was 94.87%.Conclusions: Fine Needle Aspiration Cytology is thus a safe, reliable, quick, convenient and accurate method of diagnosis and should be considered as one of the first line of investigations in the evaluation of salivary gland lesions.

scholarly journals Medical thoracoscopy in evaluation of undiagnosed pleural effusion

2019 ◽  
Vol 7 (5) ◽  
pp. 1803
Author(s):  
Khurshid Ahmad Dar ◽  
Sheikh Tariq Sultan ◽  
Ahmed Jamal Jamil ◽  
Nazia Mehfooz ◽  
Naveed Nazir Shah ◽  
...  
Keyword(s):  
Pleural Effusion ◽  
Diagnostic Yield ◽  
Cost Effective ◽  
Histopathological Diagnosis ◽  
Complication Rates ◽  
Safe Procedure ◽  
Diagnostic Modality ◽  
Medical Thoracoscopy ◽  
Multiple Variable ◽  
Age Range

Background: Medical thoracoscopy or pleuroscopy, in recent past has received lot of interest for diagnostic as well as therapeutic purposes. In the evaluation of undiagnosed pleural effusion, it has become a key diagnostic modality as it is a cost effective and safe procedure. The aim of present study was to assess the diagnostic yield of medical thoracoscopy in patients with undiagnosed exudative pleural effusion.Methods: This prospective study was conducted at government chest diseases hospital Srinagar between December 2016 to June 2018. One hundred and twenty-five (125) patients who fulfilled inclusion criteria were included in this study. Thoracoscopy was done using rigid thoracoscope under local anesthesia.  Thoracoscopic and histopathological data of enrolled patients was collected prospectively and analysed.Results: Patients enrolled in the study were in the age range of 17 to 82 years and consisted of 80 males and 45 females. Most common thoracoscopic finding was multiple variable sized nodules (53.6%) followed by sago grain infiltration (15.2%). Malignancy was the most common histopathological diagnosis (60.8%) with metastatic adenocarcinoma being the most common histopathological diagnosis (50%). The overall diagnostic yield of thoracoscopy was 90.4%.Conclusions: Medical thoracoscopy is a safe procedure with excellent diagnostic yield for evaluation of undiagnosed pleural effusion with minimal complication rates.

scholarly journals A prospective study and correlation of cytological, cell-block and histopathological diagnosis of breast lesions

2021 ◽  
Vol 8 (3) ◽  
pp. 352-358
Author(s):  
Ankita Paul ◽  
Sowmya S Manjunath
Keyword(s):  
Prospective Study ◽  
Histopathological Examination ◽  
Needle Aspiration ◽  
Histopathological Diagnosis ◽  
Cell Block ◽  
Diagnostic Modality ◽  
Cross Sectional ◽  
Diagnostic Role ◽  
Palpable Breast ◽  
A Prospective Study

Breast carcinoma is the second most common cancer in the world and establishing an early diagnosis is clinically very important. Fine needle aspiration cytology (FNAC) is the fast, reliable and ideal initial diagnostic modality for the diagnosis of these lesions. This is a cross-sectional prospective study of 12 months duration. A total of 160 patients underwent FNAC of palpable breast lump/lumps. A cell-block preparation was done for 80 cases and the histopathological examination has been performed on all the 160 cases.The cytomorphological diagnosis, cell-block diagnosis and histopathological diagnosis were correlated using suitable statistical methods. Out of 160 patients, on cytological examination, 107 cases (67%) were benign and 53cases (33%) were malignant. On histopathological examination, 104 cases (65%) were benign and 56 cases (35%) were malignant. On examination of cell block, two cases which were indeterminate in FNAC turned out to be malignant. FNAC plays a main diagnostic role as an out-patient procedure which gives rapid diagnosis. FNAC when combined with cell-block can give more accurate diagnosis.

scholarly journals Diagnostic yield of transbronchial needle aspiration for lymphoma

2016 ◽  
Vol 22 (3) ◽  
pp. 67
Author(s):  
B Sonnekus ◽  
J Steenkamp ◽  
M Louw ◽  
C F N Koegelenberg
Keyword(s):  
Predictive Value ◽  
Diagnostic Yield ◽  
Cost Effective ◽  
Final Diagnosis ◽  
Needle Aspiration ◽  
Tracheobronchial Tree ◽  
Transbronchial Needle Aspiration ◽  
Predictive Values ◽  
Hilar Adenopathy ◽  
Sensitivity Specificity

<p>Background. Transbronchial needle aspiration (TBNA) is a minimally invasive bronchoscopic technique that is cost-effective and safe for diagnosing mediastinal and hilar adenopathy in lung cancer, other malignancies, sarcoidosis and infectious processes such as tuberculosis. Few studies have analysed the sensitivity, specificity and predictive values of TBNA for diagnosing lymphoma.</p><p>Objective. To evaluate the diagnostic yield of TBNA for diagnosing mediastinal and hilar adenopathy in suspected lymphoma.</p><p>Methods. We performed a retrospective analysis of collected data of patients with mediastinal and hilar adenopathy adjacent to the tracheobronchial tree detected by thoracic computed tomography, who underwent TBNA at Tygerberg Hospital between July 2010 and June 2013. We included 25 patients with suspected or proven lymphoma. Histology was used as the gold standard.</p><p>Results. Adequate samples for cytological evaluation were obtained for 22 (88%) patients. Cytological diagnosis was possible for 8 (32%). For 17 (68%) who could not be diagnosed by TBNA alone, histology provided final diagnosis. Rapid on-site examination (ROSE) was performed in 23 (92%). In 17/23 (74%) cases, these had similar results to formal cytology. Only 4 (16%) had flow cytometry requested. Twelve (48%) had lymphoma confirmed on histology. TBNA cytology had 100% specificity and positive predictive value for suspicion of lymphoma. Sensitivity was 33% and negative predictive value 62%.</p><p>Conclusion. TBNA is an appropriate first-line diagnostic procedure in evaluating mediastinal and hilar lymphadenopathy in suspected lymphoma. Biopsy should be the immediate second-line procedure when ROSE/cytology is suspicious of lymphoma or shows atypical cells. Patients with negative TBNA cytology, but high clinical or radiological suspicion of lymphoma, should be further investigated.</p>

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