scholarly journals Obtaining viable Azospirillum brasilense SR80 cells encapsulated in alginate hydrogel

2021 ◽  
Vol 21 (3) ◽  
pp. 298-303
Author(s):  
Aleksandr V. Kovrizhnikov ◽  
◽  
Timofey E. Pylaev ◽  
Andrey M. Zaharevich ◽  
Svetlana A. Konnova ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Industrial Production ◽  
Azospirillum Brasilense ◽  
Respiratory Activity ◽  
Alginate Beads ◽  
Alginate Hydrogel ◽  
Bacterial Preparations ◽  
Ca Alginate ◽  
Growth Ability ◽  
Immobilization Method

Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding. We demonstrated the retained respiratory activity and growth ability of the bacteria during immobilization, thus confirming the advantageous prospects of alginate templates for azospirilla encapsulation.

Fabrication of monodisperse alginate microgel beads by microfluidic picoinjection: A chelate free approach

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Husnain Ahmed ◽  
Bjørn Torger Stokke
Keyword(s):  
Calcium Alginate ◽  
Biomedical Applications ◽  
Alginate Beads ◽  
Alginate Hydrogel ◽  
Hydrogel Beads ◽  
Ca Alginate

Micron-sized alginate hydrogel beads are extensively employed as an encapsulation medium for biochemical and biomedical applications. Here we report on the microfluidic assisted fabrication of calcium alginate (Ca-alginate) beads by...

Characterization and Screening of a Novel Multiparticulate Pulsatile Delivery of Aceclofenac

2016 ◽  
Vol 9 (5) ◽  
pp. 3494-3501
Author(s):  
Bipul Nath ◽  
Santimoni Saikia
Keyword(s):  
Drug Release ◽  
Sodium Alginate ◽  
Alginate Beads ◽  
In Vitro Drug Release ◽  
Dsc Studies ◽  
Ft Ir ◽  
Lag Period ◽  
Pulsatile Delivery ◽  
Ca Alginate

In the present investigation, sodium alginate based multiparticulate system overcoated with time and pH dependent polymer was studied in the form of oral pulsatile system to achieve pulsatile with sustained release of aceclofenac for chronotherapy of rheumatoid arthritis seven batches of micro beads with varying concentration of sodium alginate (2-5 %) were prepared by ionotropic-gelation method using CaCl2 as cross-linking agent. The prepared Ca-alginate beads were coated with 5% Eudragit L100 and filled into pulsatile capsule with varying proportion of plugging materials. Drug loaded microbeads were investigated for physicochemical properties and drug release characteristics. The mean particle sizes of drug-loaded microbeads were found to be in the range 596±1.1 to 860 ± 1.2 micron and %DEE in the range of 65-85%. FT-IR and DSC studies revealed the absence of drug polymer interactions. The release of aceclofenac from formulations F1 to F7 in buffer media (pH 6.8) at the end of 5h was 65.6, 60.7, 55.7, 41.2, 39.2, 27 and 25% respectively. Pulsatile system filled with eudragit coated Ca-alginate microbeads (F2) showed better drug content, particle size, surface topography, in-vitro drug release in a controlled manner. Different plugging materials like Sterculia gum, HPMC K4M and Carbopol were used in the design of pulsatile capsule. The pulsatile system remained intact in buffer pH 1.2 for 2 hours due to enteric coat of the system with HPMCP. The enteric coat dissolved when the pH of medium was changed to 7.4. The pulsatile system developed with Sterculia gum as plugging material showed satisfactory lag period when compared to HPMC and Carbopol.

Immobilization of horseradish peroxidase in Ca-alginate beads: Evaluation of the enzyme leakage on the overall removal of an azo-dye and mathematical modeling

2021 ◽  
Vol 156 ◽  
pp. 134-143
Author(s):  
Diego Alberto Morales Urrea ◽  
Analía Verónica Fernández Gimenez ◽  
Yamila Eliana Rodriguez ◽  
Edgardo Martín Contreras
Keyword(s):  
Mathematical Modeling ◽  
Horseradish Peroxidase ◽  
Azo Dye ◽  
Alginate Beads ◽  
Enzyme Leakage ◽  
Ca Alginate

Use of Immobilized Candida Cells on Xylitol Production from Sugarcane Bagasse

2000 ◽  
Vol 55 (3-4) ◽  
pp. 213-217 ◽  
Author(s):  
Walter de Carvalho ◽  
Silvio Silvério da Silva ◽  
Michele Vitolo ◽  
Ismael Maciel de Mancilha
Keyword(s):  
Sugarcane Bagasse ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Candida Guilliermondii ◽  
Volumetric Productivity ◽  
Xylitol Production ◽  
Experimental Conditions ◽  
Work Volume ◽  
Yield Factor ◽  
Ca Alginate

Abstract In this study we used the yeast Candida guilliermondii FTI 20037 immobilized by entrapment in Ca-alginate beads (2 .5 -3 mm diameter) for xylitol production from concentrated sugarcane bagasse hemicellulosic hydrolysate in a repeated batch system. The fermentation runs were carried out in 125- and 250-ml Erlenmeyer flasks placed in an orbital shaker at 30 °C and 200 rpm during 72 h, keeping constant the proportion between work volume and flask total volume. According to the results, cell viability was substantially high (98%) in all fermentative cycles. The values of parameters xylitol yield and volumetric productivity increased significantly with the reutilization of the immobilized biocatalysts. The highest values of xylitol final concentration (11.05 g/1), yield factor (0.47 gig) and volumetric productivity (0.22 g/lh) were obtained in 250-ml Erlenmeyer flasks containing 80 ml of medium plus 20 mi of immobilized biocatalysts. The support used in this study (Ca-alginate) presented stability in the experimental conditions used. The results show that the use of immobilized cells is a promising approach for increasing the xylitol production rates.

Bacterial behaviour in the biodegradation of phenol by indigenous bacteria immobilized in Ca-alginate beads

2018 ◽  
Vol 41 (14) ◽  
pp. 1829-1836
Author(s):  
A. Namane ◽  
F. Amrouche ◽  
J. Arrar ◽  
O. Ali ◽  
A. Hellal
Keyword(s):  
Alginate Beads ◽  
Indigenous Bacteria ◽  
Ca Alginate

scholarly journals Development of a bioreactor system for the remediation of endosulphan

2018 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
K. Jesitha ◽  
P. S. Harikumar
Keyword(s):  
Detection Limit ◽  
Calcium Alginate ◽  
Packed Bed ◽  
Alginate Beads ◽  
Soil Samples ◽  
Packed Bed Reactor ◽  
Contaminated Water ◽  
Initial Concentration ◽  
Bioreactor System ◽  
Ca Alginate

Abstract A bioreactor system that consisted of Pseudomonas fluorescens cells immobilised in calcium-alginate beads was utilised to remediate endosulphan contaminated water and soil. A packed bed reactor system was designed for the bio-degradation of endosulphan in artificially spiked water samples (initial concentration of endosulphan: 350 µg/L). Reactor studies with cell-immobilised Ca-alginate beads were conducted after checking their efficiency through batch and column degradation studies. The results showed that the concentration of toxic isomers of endosulphan (endosulphan alpha and endosulphan beta) was below the limit in the bioreactor during the 7th day of the experiment. Experiments conducted with contaminated soil samples (initial concentration of endosulphan: 1,000 μg/kg) indicated that the toxic isomers of endosulphan degraded to below the detection limit within 10 days and monitoring of endosulphan residues on the 14th day revealed that almost complete degradation of metabolites of endosulphan had occurred. The bioreactor system designed can be scaled up for remediation of endosulphan in contaminated areas.

High ethanol productivities using small Ca-alginate beads of immobilized cells of Zymomonas mobilis

1981 ◽  
Vol 3 (11) ◽  
pp. 613-618 ◽  
Author(s):  
Argyrios Margaritis ◽  
Pramod K. Bajpai ◽  
J. Blair Wallace
Keyword(s):  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
High Ethanol ◽  
Ca Alginate

Ultrastructure of interaction in alginate beads between the microalgaChlorella vulgariswith its natural associative bacteriumPhyllobacterium myrsinacearumand with the plant growth-promoting bacteriumAzospirillum brasilense

2001 ◽  
Vol 47 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Vladimir K Lebsky ◽  
Luz E Gonzalez-Bashan ◽  
Yoav Bashan
Keyword(s):  
Wastewater Treatment ◽  
Plant Growth ◽  
Azospirillum Brasilense ◽  
Bacterial Species ◽  
Alginate Beads ◽  
Transmission Electron ◽  
Associative Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Treatment Water

Chlorella vulgaris, a microalga often used in wastewater treatment, was coimmobilized and coincubated either with the plant growth-promoting bacterium Azospirillum brasilense, or with its natural associative bacterium Phyllobacterium myrsinacearum, in alginate beads designed for advanced wastewater treatment. Interactions between the microalga and each of the bacterial species were followed using transmission electron microscopy for 10 days. Initially, most of the small cavities within the beads were colonized by microcolonies of only one microorganism, regardless of the bacterial species cocultured with the microalga. Subsequently, the bacterial and microalgal microcolonies merged to form large, mixed colonies within the cavities. At this stage, the effect of bacterial association with the microalga differed depending on the bacterium present. Though the microalga entered a senescence phase in the presence of P. myrsinacearum, it remained in a growth phase in the presence of A. brasilense. This study suggests that there are commensal interactions between the microalga and the two plant associative bacteria, and that with time the bacterial species determined whether the outcome for the microalga is senescence or continuous multiplication.Key words: Azospirillum, Chlorella, Phyllobacterium, wastewater treatment, water bioremediation.

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