scholarly journals The Effect of The Shape of Magnetic Field on the Viability of Endothelial Cells

2021 ◽  
Author(s):  
Akram Mahna ◽  
Saeed Solali ◽  
Fateme Akbarbeiglou
Keyword(s):  
Magnetic Field ◽  
Endothelial Cells ◽  
Magnetic Fields ◽  
Chronic Exposure ◽  
Umbilical Vein ◽  
Non Invasive ◽  
Effective Factor ◽  
Umbilical Vein Endothelial Cells ◽  
Acute And Chronic Exposure ◽  
The Magnetic Field

Purpose: Magnetic field is one of the effective and non-invasive modalities on biology and angiogenesis. Studies on the effects of magnetic fields on angiogenesis showed that the shape of the magnetic field could potentially affect angiogenesis. Therefore, this study aimed to control the frequency, intensity, and duration of exposure of magnetic field while investigating the effect of the shape of the magnetic field on the viability of Human Umbilical Vein Endothelial Cells (HUVECs). Materials and Methods: The HUVECs were exposed to various shapes of 50 and 60 Hz magnetic fields with intensities of 0.5 and 1 mT in acute and chronic exposure regimes. The viability of HUVECs was assessed via MTT assay. Results: Results showed that the sin type 50 and 60 Hz magnetic fields are more effective in decreasing the viability. The rectified 100 and 120 Hz with 1 and 0.5 mT could increase and decrease the viability compared with 50 and 60 Hz, respectively. Conclusion: It can be concluded that the shape of the magnetic field can be an effective factor in biology and must be controlled to have a reliable response.

Gene expression profile in cultured human umbilical vein endothelial cells exposed to a 300 mT static magnetic field

2011 ◽  
Vol 33 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Emanuela Polidori ◽  
Sabrina Zeppa ◽  
Lucia Potenza ◽  
Chiara Martinelli ◽  
Evelin Colombo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Magnetic Field ◽  
Endothelial Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Static Magnetic Field ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Effects of a 300 mT static magnetic field on human umbilical vein endothelial cells

2010 ◽  
Vol 31 (8) ◽  
pp. 630-639 ◽  
Author(s):  
Lucia Potenza ◽  
Chiara Martinelli ◽  
Emanuela Polidori ◽  
Sabrina Zeppa ◽  
Cinzia Calcabrini ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Endothelial Cells ◽  
Static Magnetic Field ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Emergence of Twisted Magnetic Flux Related Sigmoidal Brightening

2000 ◽  
Vol 179 ◽  
pp. 263-264
Author(s):  
K. Sundara Raman ◽  
K. B. Ramesh ◽  
R. Selvendran ◽  
P. S. M. Aleem ◽  
K. M. Hiremath
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Magnetic Flux ◽  
Ultra Violet ◽  
Active Regions ◽  
Morphological Properties ◽  
Energy Storage And Conversion ◽  
The Sun ◽  
X Rays ◽  
The Magnetic Field

Extended AbstractWe have examined the morphological properties of a sigmoid associated with an SXR (soft X-ray) flare. The sigmoid is cospatial with the EUV (extreme ultra violet) images and in the optical part lies along an S-shaped Hαfilament. The photoheliogram shows flux emergence within an existingδtype sunspot which has caused the rotation of the umbrae giving rise to the sigmoidal brightening.It is now widely accepted that flares derive their energy from the magnetic fields of the active regions and coronal levels are considered to be the flare sites. But still a satisfactory understanding of the flare processes has not been achieved because of the difficulties encountered to predict and estimate the probability of flare eruptions. The convection flows and vortices below the photosphere transport and concentrate magnetic field, which subsequently appear as active regions in the photosphere (Rust & Kumar 1994 and the references therein). Successive emergence of magnetic flux, twist the field, creating flare productive magnetic shear and has been studied by many authors (Sundara Ramanet al.1998 and the references therein). Hence, it is considered that the flare is powered by the energy stored in the twisted magnetic flux tubes (Kurokawa 1996 and the references therein). Rust & Kumar (1996) named the S-shaped bright coronal loops that appear in soft X-rays as ‘Sigmoids’ and concluded that this S-shaped distortion is due to the twist developed in the magnetic field lines. These transient sigmoidal features tell a great deal about unstable coronal magnetic fields, as these regions are more likely to be eruptive (Canfieldet al.1999). As the magnetic fields of the active regions are deep rooted in the Sun, the twist developed in the subphotospheric flux tube penetrates the photosphere and extends in to the corona. Thus, it is essentially favourable for the subphotospheric twist to unwind the twist and transmit it through the photosphere to the corona. Therefore, it becomes essential to make complete observational descriptions of a flare from the magnetic field changes that are taking place in different atmospheric levels of the Sun, to pin down the energy storage and conversion process that trigger the flare phenomena.

Single-Chain and Two-Chain Tissue-Type Plasminogen Activator (t-PA) Bind Differently to Cultured Human Endothelial Cells

1989 ◽  
Vol 62 (02) ◽  
pp. 699-703 ◽  
Author(s):  
Rob J Aerts ◽  
Karin Gillis ◽  
Hans Pannekoek
Keyword(s):  
Endothelial Cells ◽  
Plasminogen Activator ◽  
Binding Sites ◽  
Umbilical Vein ◽  
Tissue Type ◽  
Single Chain ◽  
Human Endothelial Cells ◽  
Tissue Type Plasminogen Activator ◽  
Type Plasminogen Activator ◽  
Umbilical Vein Endothelial Cells

SummaryIt has recently been shown that the fibrinolytic components plasminogen and tissue-type plasminogen activator (t-PA) both bind to cultured human umbilical vein endothelial cells (HUVEC). After cleavage of t-PA by plasmin, “single-chain” t-PA (sct-PA) is converted into “two-chain” t-PA (tct-PA), which differs from the former in a number of respects. We compared binding of sct-PA and tct-PA to the surface of HUVEC. Removal of t-PA bound to HUVEC by a mild treatment with acid and a subsequent quantification of eluted t-PA both by activity- and immunoradiometric assays revealed that, at concentrations between 10 and 500 nM, HUVEC bind about 3-4 times more sct-PA than tct-PA. At these concentrations, both sct-PA and tct-PA remain active when bound to HUVEC. Mutual competition experiments showed that sct-PA and tct-PA can virtually fully inhibit binding of each other to HUVEC, but that an about twofold higher concentration of tct-PA is required to prevent halfmaximal binding of sct-PA than visa versa. These results demonstrate that sct-PA and tct-PA bind with different affinities to the same binding sites on HUVEC.

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