scholarly journals Pyrrolidinedithiocarbamic Acid Ammonium Salt Inhibits Apop-tosis and Phenotypic Transformation of Co-Culture of Myeloma Cells and Renal Tubular Epithelial Cells by Reducing the Secretion of Light Chain Protein

2020 ◽  
Author(s):  
Xiaoyan YU ◽  
Jie BAO ◽  
Xinyu CUI ◽  
Fengxia DU ◽  
Yuefei WANG ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ammonium Salt ◽  
Light Chain ◽  
Culture System ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Myeloma Cells ◽  
Λ Light Chain ◽  
Renal Tubular ◽  
Rpmi 8226

Background: We investigate the effects of NFκB inhibitor pyrrolidinedithiocarbamic acid ammonium salt (PDTC) on the viability, apoptosis and cell phenotype of HK-2 cells in the co-culture system of myeloma cells in renal tubular epithelial cells. Methods: This study was performed in Qiqihar Medical University, Qiqihar, China from Jun 2018 to Jan 2019. RPMI-8226 cells and HK-2 cells were inoculated in the co-culture chamber and cultured to establish the co-culture system. An immunoturbidimetric assay was performed to detect κ light chain and λ light chain in RPMI-8226 cells. The effect of PDTC on the secretion of κ light chain and λ light chain of RPMI-8226 cells was detected by immunoturbidimetry and the ratio was calculated. Results: PDTC significantly increased the viability of HK-2 cells. PDTC reduced the apoptosis of renal tubular epithelial cells. After PDTC treatment, the expression of cell surface marker E-cadherin decreased, and the expression of α-SMA increased, which induced the renal interstitial fibrosis. The secretion of κ light chain and λ light chain of RPMI-8226 cells was significantly decreased after the addition of PDTC, but the ratio was not changed. Conclusion: PDTC can inhibit the cell activity, promote apoptosis, and reduce the secretion of secretion of κ light chain and λ light chain through inhibiting the NF-κB pathway activation of myeloma cell RPMI-8226.

PDTC inhibits apoptosis and phenotypic transformation of co-culture of myeloma cells and renal tubular epithelial cells by reducing the secretion of light chain protein

2020 ◽  
Author(s):  
Xiaoyan Yu ◽  
Jie Bao ◽  
Xinyu Cui ◽  
Fengxia Du ◽  
Yuefei Wang
Keyword(s):  
Epithelial Cells ◽  
Light Chain ◽  
Culture System ◽  
Interstitial Fibrosis ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Myeloma Cells ◽  
Λ Light Chain ◽  
Renal Tubular ◽  
Rpmi 8226

Abstract Background The human myeloma cell line RPMI-8226 is co-cultured with human proximal tubular epithelial cell HK-2, which is commonly used to simulate the renal environment in patients with multiple myeloma. This study aimed to investigate the effects of NFκB inhibitor PDTC on the viability, apoptosis and cell phenotype of HK-2 cells in the co-culture system of myeloma cells in renal tubular epithelial cells.Methods An in vivo environment was simulated through a cell co-culture system. RPMI-8226 cells and HK-2 cells were inoculated in the co-culture chamber and cultured for 24 h to establish the co-culture system. PDTC was used in the single culture group and the co-culture group, respectively. The activity of HK-2 cells and RPMI-8226 cells in each group was detected by MTT. An immunoturbidimetric assay was performed to assess the effect of PDTC on secretion of κ light chain and λ light chain in RPMI-8226 cells. Flow cytometry was used to detect apoptosis of HK-2 cells. Western blot was carried out to detect NF-κB activation in RPMI-8226 myeloma cells, as well as the expression levels of caspase-3, bcl-2, Bax, E-cadherin, and α-SMAin HK-2 cells. Caspse-3 assay kit was used to detect the activity of caspase-3. The effect of PDTC on the secretion of κ light chain and λ light chain of RPMI-8226 cells was detected by immunoturbidimetry and the ratio was calculated.Results PDTC had a potent inhibitory effect on proliferation of RPMI-8226 cells in a dose- and time-dependent manner. PDTC had no significant effect on the viability of HK-2 cells cultured alone, and the addition of PDTC to the co-culture system significantly increased the viability of HK-2 cells. PDTC did not significantly change the apoptosis of HK-2 cells cultured alone, but could reduce the apoptosis of renal tubular epithelial cells by regulating the activity of caspase3 and the ratio of bcl2/bax in HK-2 cells in the co-culture system. After PDTC treatment, the expression of cell surface marker E-cadherin decreased, and the expression of α-SMA increased, which induced the renal interstitial fibrosis. The secretion of κ light chain and λ light chain of RPMI-8226 cells was significantly decreased after the addition of PDTC, but the ratio was not changed.Conclusions PDTC can inhibit the cell activity, promote apoptosis, and reduce the secretion of secretion of κ light chain and λ light chain through inhibiting the NF-κB pathway activation of myeloma cell RPMI-8226, leading to increased activity of renal tubular epithelial cells HK-2 in the co-culture system, decreased apoptosis, and renal interstitial fibrosis.

scholarly journals MiR-30c regulates cisplatin-induced apoptosis of renal tubular epithelial cells by targeting Bnip3L and Hspa5

2017 ◽  
Vol 8 (8) ◽  
pp. e2987-e2987 ◽  
Author(s):  
Bin Du ◽  
Xiao-meng Dai ◽  
Shuang Li ◽  
Guo-long Qi ◽  
Guang-xu Cao ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Renal Tubular ◽  
Induced Apoptosis

Cadmium and Molybdenum co-induce pyroptosis via ROS/PTEN/PI3K/AKT axis in duck renal tubular epithelial cells

2020 ◽  
pp. 116403
Author(s):  
Caiying Zhang ◽  
Tianjin Lin ◽  
Gaohui Nie ◽  
Ruiming Hu ◽  
Shaoxing Pi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Renal Tubular

Possible reason for preferential damage to renal tubular epithelial cells evoked by amphotericin B.

1996 ◽  
Vol 40 (5) ◽  
pp. 1116-1120 ◽  
Author(s):  
I Walev ◽  
S Bhakdi
Keyword(s):  
Epithelial Cells ◽  
Amphotericin B ◽  
Important Determinant ◽  
Major Complication ◽  
Toxic Action ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Recovery Mechanisms ◽  
Renal Tubular

An important determinant of nephrotoxicity, which is the major complication of long-term amphotericin B treatment, is dysfunction of distal tubular epithelial cells. The underlying cause for this rather selective damage to the cells is unknown. In the present investigation, it was shown that kidney epithelial cells were initially damaged by amphotericin B at concentrations of 2.5 to 10 micrograms/ml, as demonstrable by a dramatic drop in cellular K+ levels. Cells could recover from the initial toxic action of the polyene if they were kept in medium of neutral pH, and cellular K+ levels returned to normal after 6 h. However, the recovery mechanisms failed at lower pHs of 5.6 to 6.0. At low pHs, cells became progressively depleted of ATP; they leaked lactate dehydrogenase and became irreversibly damaged after approximately 6 h. The possibility that the low pH characteristic of the distal tubulus lumen renders the renal epithelial cells particularly vulnerable to the toxic action of amphotericin B is raised. The concept is in line with an earlier report that alkalization ameliorates amphotericin B nephrotoxicity in rats.

Sign in / Sign up

Export Citation Format

Share Document