scholarly journals Sulforaphane response on aluminum-induced oxidative stress, alterations in sperm characterization and testicular histomorphometry in Wistar rats

2020 ◽  
Author(s):  
Babatunde Ogunlade ◽  
Sunday Adelakun ◽  
Kingsley Iteire
Keyword(s):  
Oxidative Stress ◽  
Adult Male ◽  
Antioxidant Status ◽  
Wistar Rats ◽  
Sperm Count ◽  
Seminiferous Tubules ◽  
Testicular Toxicity ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Aluminum Trichloride

Background: The exposure of male individual to environmental toxicant is regarded as a channel that results in reduced sperm counts and infertility. Objective: This study investigated the ameliorative response of Sulforaphane (SFN) on Aluminum trichloride (AlCl3) induced testicular toxicity in adult male Wistar rats. Materials and Methods: A total of 32 adult male Wistar rats (180-200 gm between 8-10 wk) were divided into four groups (n = 8/each). Group A) received distilled water orally as placebo; Group B) received 100 mg/kgbw AlCl3 only orally; Group C) received 100 mg/kgbw AlCl3 and 100 mg/kgbw SFN orally; and Group D) received 100 mg/kgbw SFN only orally. After 28 days of experiment, animals underwent cervical dislocation, blood serum was obtained for analysis, and testes were harvested for biochemical assays, histology, hormonal profile, and sperm characterization. Results: The sperm parameters showed a significant difference within the AlCl3 only group compared with the control and SFN only groups (p = 0.02). However, AlCl3 and SFN co-treatment showed improvement in the motility, viability, and sperm count compared with the AlCl3 only group (p = 0.02). Furthermore, there was a significant decline in the levels of hormones profile and antioxidant status in AlCl3 only group compared to the control and SFN only (p = 0.02). The testicular histoarchitecture of the AlCl3 only group showed shrinkage of seminiferous tubules, spermatogenesis disruption, and empty lumen compared to the control and SFN only groups. Conclusion: The present study revealed the ameliorative response of SFN on AlCl3-induced testicular toxicity on serum hormone profiles, antioxidant status, lipid peroxidation, and histomorphometric analysis through oxidative stress. Key words: Sulforaphane, Aluminum trichloride, Oxidative stress, Testis, Histology.

The effect of Achillea millefolium extract on spermatogenesis of male Wistar rats

2010 ◽  
Vol 30 (4) ◽  
pp. 328-334 ◽  
Author(s):  
Nasrin Takzare ◽  
Mir-Jamal Hosseini ◽  
Seyedeh Hamideh Mortazavi ◽  
Sahar Safaie ◽  
Rayhaneh Moradi
Keyword(s):  
Adult Male ◽  
Seminiferous Tubule ◽  
Wistar Rats ◽  
Sperm Count ◽  
Basal Membrane ◽  
Achillea Millefolium ◽  
Seminiferous Tubules ◽  
Native Plant ◽  
Cell Accumulation ◽  
Male Wistar Rats

Achillea millefolium or yarrow, a native plant in many countries, has been recognized in historical medicine, mainly because of its astringent effects. However, some aspects of the toxicity of yarrow such as possible effects on male reproductive system are not well established. In this investigation, the effects of A. millefolium L. extract on spermatogenesis in adult male wistar rats were studied. Eighty-five male Wistar rats were divided into nine experimental groups (10 in each group except the ninth group). Extract was administered at the dose of 200,400 and 800 mg/kg/day by intraperitoneal (IP) injection or through gavage for 22 days, on every other day. Three groups were determined as sham and control groups. Five rats from each group were killed and the rest of the rats were kept for 40 days later, but with no injection, to assess the reversibility of extract effect on spermatogenesis. The results of the study showed scattered immature cells on basal membrane in seminiferous tubules at the dose of 400 mg/kg/day IP. Moreover, a significant decrease in cell accumulation and vacuolization in seminiferous tubule was seen. At the dose of 800 mg/kg, IP, thickened seminiferous tubules on basal membrane, decrease in cell accumulation in seminiferous tubule, severe disarrangement, degenerative cells and severe decrease in sperm count were seen. At the dose of 800 mg/kg/day, orally, basal membrane was thickened and the disarrangement in cells was demonstrated. As a conclusion, our results suggest that the total extract of A. millefolium L. exhibit temporary antifertile activity in adult male animals.

scholarly journals Azathioprine and Methotrexate impaired the morphology and functions of the testes in adult wistar rats

2014 ◽  
Vol 31 (02) ◽  
pp. 075-081
Author(s):  
A. Akinlolu ◽  
O. Akinola ◽  
P. Khobe ◽  
K. Obasi ◽  
O. Dada
Keyword(s):  
Adult Male ◽  
Wistar Rats ◽  
Follicle Stimulating Hormone ◽  
Physiological Saline ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Connective Tissues ◽  
Group I ◽  
Male Wistar Rats ◽  
Dose Dependent

Abstract Introduction: AAzathioprine and Methotrexate are both used in the treatment of cancer; and are classified as cytotoxic drugs with reported adverse effects such as oxidative damage to the DNA/RNA, the testes and sperm cells. This study, therefore, tested the hypothesis that AAzathioprine and Methotrexate administrations impair the morphology and functions of the testes in adult male wistar rats. Methods: AAzathioprine (50-150mg per day) and Methotrexate (2.5mg per week) are used in the treatment of cancer in adult Man. We tested the hypothesis that AAzathioprine and Methotrexate impair the morphology and functions of testes in rats. Forty adult male wistar rats (150-230g) were employed in the study: Control Group I received physiological saline while Experimental Groups II - V received oral administrations of 5mg/kg/bodyweight of AAzathioprine per day, 15mg/kg/bodyweight of AAzathioprine per day, 8mg/kg/bodyweight of Methotrexate per week and 20mg/kg/bodyweight of Methotrexate per week respectively for 35 days. Results: Histological examinations of the testes of rats of Groups II - V showed dose-dependent morphological anomalies such as fewer collagen ibers of connective tissues, disrupted seminiferous tubules and scanty spermatozoa when compared to rats of Group I. Statistical analyses showed dose-dependent elevated levels (P≤0.05) of superoxide dismutase and malondialdehyde in testes homogenates of rats of Groups II - V when compared to rats of Group I. This implied increased oxidative stress in rats of Groups II - V. Evaluations of Follicle Stimulating Hormone and Testosterone showed dose-dependent significantly elevated levels (P≤0.05) in rats of Groups II - V when compared to rats of Group I. Conclusions: Our findings are consistent with the stated hypothesis.

Chronic consumption of Hypericum humifusum leaf extracts impairs epididymis spermatozoa characters in association with oxidative stress in adult male Wistar rats

2017 ◽  
Vol 93 ◽  
pp. 616-625 ◽  
Author(s):  
Imen Hammami ◽  
Ridha Ben Ali ◽  
Afef Nahdi ◽  
Olfa Kallech-Ziri ◽  
Marwa Boussada ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Male ◽  
Wistar Rats ◽  
Leaf Extracts ◽  
Male Wistar Rats

TiO2 Nanoparticles Induced Oxidative Stress Mediated DNA Damage In The Liver Of Adult Male Wistar Rats

2018 ◽  
Vol 10 (2) ◽  
pp. 145-150
Author(s):  
Dasal Vasantharaja ◽  
◽  
Venugopal Ramalingam ◽  
Shanmugam Thangapandiyan ◽  
Nagarajan Sridhar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Tio2 Nanoparticles ◽  
Adult Male ◽  
Wistar Rats ◽  
Male Wistar Rats

scholarly journals Aluminium phosphide-induced testicular toxicity through oxidative stress in Wistar rats: Ameliorative role of hesperidin

2018 ◽  
Vol 2 ◽  
pp. 239784731881279 ◽  
Author(s):  
Olusegun Kayode Afolabi ◽  
Adedoja Dorcas Wusu ◽  
Regina Ugbaja ◽  
John Olabode Fatoki
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Sperm Count ◽  
Sperm Parameters ◽  
Lipid Hydroperoxides ◽  
Testicular Toxicity ◽  
Testicular Damage ◽  
Histological Alterations ◽  
Aluminium Phosphide

The present study was designed to investigate aluminium phosphide (ALP)-induced testicular toxicity, including its effects on sperm parameters and histological alterations in Wistar rats, and the possible protective role of hesperidin (HSD). Oral administration of ALP at 1.15 mg/kg body weight (1/10 LD50) for 30 days resulted in a significant increase in testicular malondialdehyde, lipid hydroperoxides, and oxidized protein levels. These indicators of oxidative stress were accompanied by decreased activity of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase, followed by a drastic reduction in the non-enzymatic antioxidant indices of glutathione and total antioxidant capacity when compared to control. Furthermore, ALP treatment produced a marked reduction in sperm count, motility and viability while increasing abnormal sperm morphology and adverse histopathological changes in testis. Co-administration with HSD significantly ameliorated ALP-induced testicular damage by suppressing oxidative stress indices and enhancing antioxidant status while also improving the sperm parameters and histological alterations in ALP-treated rats. The results of the present study indicated that testicular toxic effects of ALP are due to oxidative imbalance and that HSD could be a potential therapeutic agent against ALP-induced testicular damage.

scholarly journals The Effects of Long-Term Chaetomellic Acid A Administration on Renal Function and Oxidative Stress in a Rat Model of Renal Mass Reduction

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
António Nogueira ◽  
Francisco Peixoto ◽  
Maria Manuel Oliveira ◽  
Carlos André Pires ◽  
Bruno Colaço ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Renal Mass ◽  
Wistar Rats ◽  
Reduced Glutathione ◽  
Mass Reduction ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
And Oxidative Stress

Purpose.This study aimed to evaluate the effect of chronic treatment with chaetomellic acid A (CAA) on oxidative stress and renal function in a model of renal mass reduction.Methods. Male Wistar rats were subjected to 5/6 nephrectomy (RMR) or sham-operated (SO). One week after surgery, rats have been divided into four experimental groups: RMR: RMR rats without treatment(n=14); RMR + CAA: RMR rats treated with CAA(n=13); SO: SO rats without treatment(n=13); and SO + CAA: SO rats treated with CAA(n=13). CAA was intraperitoneally administered in a dose of 0.23 µg/Kg three times a week for six months.Results.RMR was accompanied by a significant reduction in catalase and glutathione reductase (GR) activity(p<0.05)and a decrease in reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio. CAA administration significantly increased catalase and GR activity(p<0.05)and increased GSH/GSSG ratio, but no significant difference between the treated and nontreated groups was found in this ratio. No significant differences were found between the RMR groups in any of the parameters of renal function. However, CAA administration slightly improves some parameters of renal function.Conclusions.These data suggest that CAA could attenuate 5/6 RMR-induced oxidative stress.

scholarly journals Interventions of aqueous extract of Solanum melongena fruits (garden eggs) on mercury chloride induced testicular toxicity in adult male Wistar rats

2020 ◽  
Vol 43 (2) ◽  
pp. 174-182 ◽  
Author(s):  
Sunday Aderemi Adelakun ◽  
Victor Okoliko Ukwenya ◽  
Grace Temitope Akingbade ◽  
Olusegun Dare Omotoso ◽  
Julius Akomaye Aniah
Keyword(s):  
Aqueous Extract ◽  
Adult Male ◽  
Wistar Rats ◽  
Solanum Melongena ◽  
Mercury Chloride ◽  
Testicular Toxicity ◽  
Male Wistar Rats
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