scholarly journals Morphological and Biochemical Parameters of Pigs’ Blood with Enzootic Pneumonia

2021 ◽  
Author(s):  
Artur Tazayan ◽  
Timur Tambiev ◽  
Tatiana Babkina
Keyword(s):  
Biochemical Parameters ◽  
Mycoplasma Hyopneumoniae ◽  
Chain Reaction ◽  
Enzootic Pneumonia ◽  
Blood Sedimentation ◽  
Secondary Infections ◽  
Blood Sedimentation Rate ◽  
Polymerase Chain ◽  
Hematological Changes ◽  
Increased Activity

One of the most common diseases of pigs in large pig enterprises is enzootic pneumonia. The main etiological agent causing enzootic pneumonia is Mycoplasma hyopneumoniae. Mycoplasmas have minimal virulence, but they increase the susceptibility of pigs to secondary infections. In association with other microorganisms, Mycoplasma hyopneumoniae enhances its pathogenic effect, which makes the course of the disease more severe, often leading to the death of animals. Blood tests were performed to study the changes in morphological and biochemical parameters during the development of infection caused by Mycoplasma hyopneumoniae. Experimental groups of animals were formed, consisting of healthy as well as infected and ill pigs of different ages (2, 3, and 4 months), in which the pathogen M. hyopneumoniae was detected by polymerase chain reaction. The following hematological changes were revealed in the piglets suffering from respiratory mycoplasmosis: erythropenia, leukopenia, increased blood sedimentation rate (BSR), hypoproteinemia, hypoalbuminemia, increased activity of alanine aminotransferase and alkaline phosphatase, increased urea, and hyperglycemia. Keywords: pigs breeding; pneumonia; biochemical parameters; morphological parameters

scholarly journals Clinical Characteristics, Serum Biochemical Changes, and Expression Profile of Serum Cfa-miRNAs in Dogs Confirmed to Have Congenital Portosystemic Shunts Accompanied by Liver Pathologies

2020 ◽  
Vol 7 (2) ◽  
pp. 35 ◽  
Author(s):  
Ahmed M. El-Sebaey ◽  
Pavel N. Abramov ◽  
Fatma M. Abdelhamid
Keyword(s):  
Biochemical Parameters ◽  
Chain Reaction ◽  
Non Invasive ◽  
Molecular Events ◽  
Portosystemic Shunts ◽  
Liver Injuries ◽  
Novel Biomarkers ◽  
Serum Biochemical ◽  
Healthy Dogs ◽  
Polymerase Chain

Computed tomography angiography (CTA) and biochemical parameters cannot specify liver pathologies in dogs with congenital portosystemic shunts (CPSS) that are easily determined by invasive histopathology. This study aims to assess the possibility of using circulating serum canine familiaris (cfa) microRNAs (miRNAs) as novel non-invasive serum-based fingerprints for liver injuries associated with various morphologies of extrahepatic and intrahepatic portosystemic shunts (EHPSS and IHPSS). Data were obtained from 12 healthy dogs and 84 dogs confirmed to have EHPSS (splenocaval, splenophrenic, splenoazygos, right gastrocaval (RGC), right gastrocaval with caudal loop (RGC–CL)) and IHPSS (right divisional and left divisional) using CTA. Hepatic pathologies were determined by histopathology. Serum expression of miRNAs was assessed by real-time polymerase chain reaction. Based on the nature of liver injuries in each shunt type, cfa-miR-122 was significantly upregulated in all CPSS groups. Meanwhile, serums cfa-miR-34a and 21 were not significantly expressed in splenophrenic or splenoazygos groups, but they were extensively upregulated in splenocaval, RGC, RGC–CL groups and less frequently in right or left divisional groups. Also, serum cfa-miR126 was significantly upregulated in both IHPSS groups but less significantly expressed in RGC, RGC–CL, and splenocaval groups. Overall, estimated cfa-miRNAs could serve as novel biomarkers to mirror the histopathological and molecular events within the liver in each shunt type.

scholarly journals Diagnosis of feline haemoplasma infection using a real-time PCR assay

2004 ◽  
Vol 75 (2) ◽  
Author(s):  
R.G. Lobetti ◽  
S. Tasker
Keyword(s):  
Real Time ◽  
Biochemical Parameters ◽  
Inverse Correlation ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Significant Inverse Correlation ◽  
Cytological Examination ◽  
Blood Smears ◽  
Polymerase Chain ◽  
Veterinary Laboratory

Haemobartonella felis has been reclassified within the genus Mycoplasma as Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum', collectively referred to as the feline haemoplasmas. A total of 78 cats from the Johannesburg area that had blood samples submitted to a private veterinary laboratory were tested using a real-time polymerase chain reaction (PCR) assay able to detect and distinguish the two feline haemoplasma (basonym Haemobartonella) species. All samples had been diagnosed with haemoplasma infection by cytological examination of blood smears. Statistical analysis was performed to evaluate associations between haemoplasma status, age, and haematological and biochemical parameters. On PCR assay 43 cats (55 %) were haemoplasma negative, 25 (32.1 %) positive for 'Candidatus Mycoplasma haemominutum', 5 (6.4 %) positive for Mycoplasma haemofelis and 5 (6.4 %) positive for both species. Significant inverse correlation was found between the amount of M. haemofelis DNA present in the blood and the haematocrit value. Cats that were positive for M. haemofelis showed macrocytic regenerative anaemia, monocytosis and thrombocytopaenia. This report documents the existence of both haemoplasma species in cats in South Africa.

Detection of Mycoplasma hyopneumoniae DNA by the polymerase chain reaction

1991 ◽  
Vol 5 (2) ◽  
pp. 103-109 ◽  
Author(s):  
Ryô Harasawa ◽  
Kaoru Koshimizu ◽  
Osamu Takeda ◽  
Takashi Uemori ◽  
Kiyozo Asada ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycoplasma Hyopneumoniae ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Survey Of Infectious Agents Associated With Porcine Respiratory Disease Complex (PRDC) In Serbian Swine Herds Using Polymerase Chain Reaction (PCR) Detection

2015 ◽  
Vol 65 (1) ◽  
pp. 79-88 ◽  
Author(s):  
SAVIĆ Božidar ◽  
RADANOVIĆ Oliver ◽  
JOVIČIĆ Dubravka ◽  
NEŠIĆ Ksenija ◽  
IVANOVIĆ Snežana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Disease ◽  
Respiratory Virus ◽  
Mycoplasma Hyopneumoniae ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Porcine Respiratory

Abstract A retrospective study on 235 natural cases of Porcine Respiratory Disease Complex in order to determine the etiological agents, their prevalence and interrelationships was performed in Serbia. Lung tissue samples were analyzed by Polymerase Chain Reaction for the presence of Porcine circovirus type 2, Porcine reproductive and respiratory virus, Swine influenza virus, Mycoplasma hyopneumoniae, Pasteurella multocida, Actinobacillus pleuropneumoniae, Haemophilus parasuis, Streptococcus suis and Arcanobacterium pyogenes. A total of 49 different combinations of viral and bacterial pathogens were found. Five different viral and viral/Mhp co-infections were detected. Monobacterial infections were found in 150 cases and polybacterial infection was detected in 85 samples. PCV2 was the main virus detected, and Pm was the most aggressive secondary pathogen detected in PRDC. The reason for PRDC being so prevalent among Serbian pigs is most likely due to the large number of risk factors in the conventional farrow-to-finish system, compared to multi-site production systems. Therefore, measures aimed at a better control of respiratory viruses, particularly Porcine circovirus type 2 and Porcine reproductive and respiratory virus, as well as Mycoplasma hyopneumoniae infections, and adoption of rational decisions on respiratory bacterial pathogens specific therapeutic and preventive strategies at herd level, simultaneously with significant improvements on farm management should reduce the occurrence of PRDC.

scholarly journals THE ACTIVITY OF ANTIOXIDANT ENZYMES AND THE EXPRESSION OF THEIR GENES IN THE RAT BRAIN AFTER INJECTING OF VINDEBURNOL IN THE MULTIPLE SCLEROSIS MODEL

2020 ◽  
Vol 3 (3(72)) ◽  
pp. 8-11
Author(s):  
A.M. Mendzheritsky ◽  
M.V. Zhukova ◽  
L.D. Safronova
Keyword(s):  
Multiple Sclerosis ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Sod1 Gene ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Increased Activity ◽  
Cerebral Vasodilator ◽  
Experimental Allergic

The article presents the results of a study on the effectiveness of vindeburnol, a structural analogue of the cerebral vasodilator vincamine, in the model of multiple sclerosis. The drug was injected subcutaneously in rats after modeling experimental allergic encephalomyelitis at a dose of 20 μg/kg, from the first manifestation of a neurological deficit over the next 10 days. In the somatosensory cortex of animals, the activity of superoxide dismutase, glutathione peroxidase and glutathione reductase, as well as the expression level of the SOD1, GPX4, GPX6 and GSR genes using real-time polymerase chain reaction were studied. It was found that vindeburnol does not affect the expression of the SOD1 gene, as well as the activity of superoxide dismutase, while increasing the expression of the GPS4, GPS6 and GRS genes, which determines the increased activity of glutathione peroxidase and glutathione reductase in rats in the model of multiple sclerosis.

scholarly journals Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems

2008 ◽  
Vol 39 (3) ◽  
pp. 471-476 ◽  
Author(s):  
M. Yamaguti ◽  
E.E. Muller ◽  
A.I. Piffer ◽  
J.D. Kich ◽  
C.S. Klein ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycoplasma Hyopneumoniae ◽  
Chain Reaction ◽  
Respiratory Problems ◽  
Polymerase Chain

scholarly journals DETERMINATION OF DIAGNOSTIC ACCURACY OF BIOCHEMICAL PARAMETERS (CRP, LDH & FERRITIN) IN THE DIAGNOSIS OF COVID-19 IN SUSPECTED COVID CASES

2021 ◽  
Vol 71 (5) ◽  
pp. 1722-26
Author(s):  
Tayyaba Ashiq ◽  
Abdus Sattar ◽  
Nasir Uddin ◽  
Qamar Bashir ◽  
Sajida Shaheen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lactate Dehydrogenase ◽  
Diagnostic Accuracy ◽  
Biochemical Parameters ◽  
C Reactive Protein ◽  
Chain Reaction ◽  
Reactive Protein ◽  
Poor Predictor ◽  
Lactate Dehydrogenase C ◽  
Polymerase Chain

Objective: To determine the diagnostic accuracy of the Lactate Dehydrogenase, C-Reactive Protein and Ferritin in suspected patients of COVID-19. Study Design: Cross-sectional validation study. Place and Duration of Study: Pathology department of Combined Military Hospital Lahore in the month of May 2020. Methodology: We included 101 adult (>18 years) symptomatic suspected COVID-19 patients of both genders. Children, pregnant women and asymptomatic patients were excluded from study. Age, gender and results of Reverse Transcriptase Polymerase Chain Reaction, Lactate Dehydrogenase, C-Reactive Protein, ferritin were recorded. Results: Lactate Dehydrodenase had highest sensitivity (75%) with positive predictive value of 71.6% and diagnostic accuracy of 65.3% among three biochemical parameters studied. Receiver Operator Characteristic curve was studied. Area under curve of Lactate Dehydrogenase (AUC=0.65) and Ferritin (AUC=0.59) reflected their ability to prognosticate the presence of COVID19 disease. However, C-Reactive Protein (AUC=0.42) appeared to be a poor predictor of the disease. Conclusion: Raised serum Lactate Dehydrogenase (>490 U/L) and Ferritin (>152 ng/L) levels can be used to predict the Reverse Transcriptase Polymerase Chain Reaction positivity for COVID-19 in the population of suspected patients of COVID19. However, C-Reactive Protein is a poor predictor of COVID-19.

Analysis of the effects of coumarin on Lens culinaris Medik by some biochemical parameters using real-time polymerase chain reaction

2019 ◽  
Author(s):  
Burcu Yuksel ◽  
Ozlem Aksoy
Keyword(s):  
Lipid Peroxidation ◽  
Real Time ◽  
Biochemical Parameters ◽  
Lens Culinaris ◽  
Probit Analysis ◽  
Pcr Analysis ◽  
Chain Reaction ◽  
Ungerminated Seed ◽  
Biochemical Studies ◽  
Polymerase Chain

This study aimed to reveal the effects of coumarin on Lens culinaris Medik. The germinated and ungerminated seed counts of the experimental groups were determined and the EC50 value was calculated by probit analysis. In biochemical studies, catalase and superoxide dismutase enzyme activities were investigated together with lipid peroxidation and hydrogen peroxide quantities. The results demonstrated an increase in the amount of malondialdehyde, a measure of lipid peroxidation, a decrease in the amount of H2O2 and EC50 value in the CAT activity and an increase in the EC50 x 2 value. In real-time PCR analysis, three different genetic expressions related to abiotic stress (CAT, Cu / Zn SOD and Mn SOD gene expression) were examined. It was determined that coumarin caused genotoxic and biochemical damage on L. culinaris.

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