Analysis of the effects of coumarin on Lens culinaris Medik by some biochemical parameters using real-time polymerase chain reaction

This study aimed to reveal the effects of coumarin on Lens culinaris Medik. The germinated and ungerminated seed counts of the experimental groups were determined and the EC50 value was calculated by probit analysis. In biochemical studies, catalase and superoxide dismutase enzyme activities were investigated together with lipid peroxidation and hydrogen peroxide quantities. The results demonstrated an increase in the amount of malondialdehyde, a measure of lipid peroxidation, a decrease in the amount of H2O2 and EC50 value in the CAT activity and an increase in the EC50 x 2 value. In real-time PCR analysis, three different genetic expressions related to abiotic stress (CAT, Cu / Zn SOD and Mn SOD gene expression) were examined. It was determined that coumarin caused genotoxic and biochemical damage on L. culinaris.

Diagnosis of feline haemoplasma infection using a real-time PCR assay

Journal of the South African Veterinary Association ◽

10.4102/jsava.v75i2.460 ◽

2004 ◽

Vol 75 (2) ◽

Cited By ~ 15

Author(s):

R.G. Lobetti ◽

S. Tasker

Keyword(s):

Real Time ◽

Biochemical Parameters ◽

Inverse Correlation ◽

Chain Reaction ◽

Pcr Assay ◽

Significant Inverse Correlation ◽

Cytological Examination ◽

Blood Smears ◽

Polymerase Chain ◽

Veterinary Laboratory

Haemobartonella felis has been reclassified within the genus Mycoplasma as Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum', collectively referred to as the feline haemoplasmas. A total of 78 cats from the Johannesburg area that had blood samples submitted to a private veterinary laboratory were tested using a real-time polymerase chain reaction (PCR) assay able to detect and distinguish the two feline haemoplasma (basonym Haemobartonella) species. All samples had been diagnosed with haemoplasma infection by cytological examination of blood smears. Statistical analysis was performed to evaluate associations between haemoplasma status, age, and haematological and biochemical parameters. On PCR assay 43 cats (55 %) were haemoplasma negative, 25 (32.1 %) positive for 'Candidatus Mycoplasma haemominutum', 5 (6.4 %) positive for Mycoplasma haemofelis and 5 (6.4 %) positive for both species. Significant inverse correlation was found between the amount of M. haemofelis DNA present in the blood and the haematocrit value. Cats that were positive for M. haemofelis showed macrocytic regenerative anaemia, monocytosis and thrombocytopaenia. This report documents the existence of both haemoplasma species in cats in South Africa.

REAL TIME POLYMERASE CHAIN REACTION (RT-PCR) FOR DNA PIG CONTAMINATION IDENTIFICATION SAUSAGE IN SIX DISTRICT PANDEGLANG BANTEN

International Journal Mathla’ul Anwar of Halal Issues ◽

10.30653/ijma.202111.13 ◽

2021 ◽

Vol 1 (1) ◽

pp. 81-88

Author(s):

Hadi Susilo

Keyword(s):

Real Time ◽

Meat Product ◽

Meat Products ◽

Pcr Analysis ◽

Processed Meat ◽

Rt Pcr ◽

Chain Reaction ◽

Dna Purity ◽

Sausage is a meat product processed that is popular food especially in Pandeglang, Banten Province. The importance of halal certificates or the existence of the MUI (Indonesian Ulama Council) halal logo for processed meat products makes Muslim people confident to consume them. The aim this research was to identify pig DNA contamination in sausage products in six districts in Pandeglang without the MUI halal labels using RT-PCR (Real Time-Polymerase Chain Reaction). RT PCR that can calculate to pig to fill these sample free from pig contamination. This research was divided into two stage, the first stage is extracted or carried out DNA and the second stage is RT PCR analysis. The results of the DNA purity test on sausage samples had DNA purity values of 1.84-1.9 (A260 / A280) and resulted in sample concentrations ranging from 37.8 to 102.5 ng / µl. The only amplification on the FAM curve was in the positive control pig. the Cq value ranges from 30 - 31.29. The results of RT PCR on sausage samples in the district area in Pandeglang Banten did not detect the presence of pig DNA.

Comparison of Real-Time Polymerase Chain Reaction and Enzyme-Linked Immunosorbent Assay for Sensitive and Quantitative Detection of Hazelnuts in Nut Pastes

Journal of AOAC International ◽

10.5740/jaoacint.18-0017 ◽

2018 ◽

Vol 101 (6) ◽

pp. 1864-1867 ◽

Cited By ~ 1

Author(s):

Ľubica Piknová ◽

Veronika Janská ◽

Tomáš Kuchta ◽

Peter Siekel

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Sandwich Elisa ◽

Pcr Analysis ◽

Quantitative Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Chain Reaction ◽

Wide Range ◽

Order Of Magnitude ◽

Abstract Background: Hazelnuts, being a frequent agent of allergenic reactions, need to be detected in food products. Thus, it is necessary to develop and further investigate appropriate methods for detection. Objective: The aim of the study was to compare the analysis of nut pastes (peanut paste spiked with different amounts of hazelnut paste) as a model of contamination of confectionery. Methods: Real-time PCR and sandwich ELISA (RidaScreen Hazelnut Fast Kit) were used. Results: For real-time PCR, LOQ of 2 mg/kg and a quantification range from 2 to 10 000 mg/kg were determined. For ELISA, LOQ of 1 mg/kg and a quantification range from 1 to 100 mg/kg were determined. Conclusions: The comparison shows that the methods had comparable sensitivity with LOQs in the same order of magnitude. Although ELISA was slightly more sensitive, it required dilution of samples at higher concentrations of the analyte because of its narrow quantification range. Results of this study suggest that real-time PCR and ELISA are both suitable methods for the analysis of nut pastes over a wide range of concentrations. Achieved results could be useful for control as well as for technological purposes. Highlights: Real-time PCR analysis of peanut paste spiked with different amounts of hazelnut paste as a model is proposed. Sandwich ELISA analysis of peanut paste spiked with different amounts of hazelnut paste as a model is proposed. The analytical parameters of real-time PCR and ELISA methods are compared.

Real–Time Quantitative Reverse Transcriptase-Polymerase Chain Reaction (RT–PCR) Analysis of the Vitamin D Pathway in UV Irradiated Keratinocytes

Biologic Effects of Light 2001 ◽

10.1007/978-1-4615-0937-0_40 ◽

2002 ◽

pp. 403-408

Author(s):

John N. Flanagan ◽

Daniel W. Rust ◽

Vin Tangpricha ◽

Tai C. Chen ◽

Michael F. Holick

Keyword(s):

Vitamin D ◽

Reverse Transcriptase ◽

Real Time ◽

Pcr Analysis ◽

Rt Pcr ◽

Chain Reaction ◽

Transovarial transmission of dengue 1 virus in Aedes aegypti larvae: real-time PCR analysis in a Brazilian city with high mosquito population density

Canadian Journal of Microbiology ◽

10.1139/cjm-2017-0614 ◽

2018 ◽

Vol 64 (6) ◽

pp. 393-400 ◽

Cited By ~ 1

Author(s):

Alexsander Moraes ◽

Filipe C. Cortelli ◽

Taís B. Miranda ◽

Davi R. Aquino ◽

José R. Cortelli ◽

...

Keyword(s):

Aedes Aegypti ◽

Real Time ◽

Pcr Analysis ◽

Transovarial Transmission ◽

Common Disease ◽

Chain Reaction ◽

Primary Mechanism ◽

Virus Serotype ◽

Transovarial transmission is among the reported factors able to influence environmental maintenance of dengue virus (DENV). Endemic areas with active transmission of dengue are suitable for studying transovarial transmission. Brazil is a country where dengue is endemic and where DENV-1 is the most common disease-related virus serotype. This study aimed to identify transovarial transmission of DENV-1 in Aedes aegypti larvae by reverse-transcriptase nested real-time polymerase chain reaction. Between March and October 2016, Culicidae larvae were collected using traps in 3 locations in Taubaté, São Paulo, Brazil, which has a high occurrence of dengue. The collected larvae were sacrificed in the 3rd or 4th larval stage, classified, and stored at –20 °C. The A. aegypti larvae samples (n = 910) were separated into 91 pools of 10 specimens each from which RNA was extracted, reverse transcribed into cDNA, and analyzed by nested qPCR. None of the pools tested positive for DENV-1. Due to the absence of detectable virus in the evaluated samples, we concluded that transovarial transmission may not be the primary mechanism for maintenance of DENV-1 in this particular environment.

Study of TMPRSS2-ETS molecular translocations in prostate cancer by fluorescence in-situ hybridization and real time polymerase chain reaction and its correlation with clinical and biochemical parameters

European Urology Supplements ◽

10.1016/s1569-9056(18)33766-7 ◽

2018 ◽

Vol 17 (14) ◽

pp. e2907

Author(s):

S. Menon ◽

S. Balagangadharan ◽

O. Shetty ◽

P. Bapat ◽

S.B. Desai

Keyword(s):

Prostate Cancer ◽

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Real Time ◽

Biochemical Parameters ◽

Chain Reaction ◽

Real-time PCR analysis of carbon catabolite repression of cellobiose dehydrogenase gene transcription inTrametes versicolor

Canadian Journal of Microbiology ◽

10.1139/w03-108 ◽

2004 ◽

Vol 50 (2) ◽

pp. 113-119 ◽

Cited By ~ 4

Author(s):

P C Stapleton ◽

J O'Mahony ◽

A D.W Dobson

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Carbon Catabolite Repression ◽

Carbon Sources ◽

Cellobiose Dehydrogenase ◽

Pcr Analysis ◽

Chain Reaction ◽

Dehydrogenase Gene ◽

Repressive Effect ◽

Cellobiose dehydrogenase production in Trametes versicolor is repressed when additional carbon sources, such as glucose, maltose, galactose, arabinose, and xylose, are added to the fungal cultures growing on cellulose. Real-time quantitative reverse transcription – polymerase chain reaction has been used to demonstrate that the addition of galactose, arabinose, and xylose results in 19-, 92-, and 114-fold reductions, respectively, in cdh transcript levels 96 h post-addition. Glucose exhibits the greatest repressive effect, resulting in a 3400-fold decrease in cdh transcript levels.Key words: cellobiose dehydrogenase, carbon repression, real-time PCR.