scholarly journals THE ACTIVITY OF ANTIOXIDANT ENZYMES AND THE EXPRESSION OF THEIR GENES IN THE RAT BRAIN AFTER INJECTING OF VINDEBURNOL IN THE MULTIPLE SCLEROSIS MODEL

2020 ◽  
Vol 3 (3(72)) ◽  
pp. 8-11
Author(s):  
A.M. Mendzheritsky ◽  
M.V. Zhukova ◽  
L.D. Safronova
Keyword(s):  
Multiple Sclerosis ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Sod1 Gene ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Increased Activity ◽  
Cerebral Vasodilator ◽  
Experimental Allergic

The article presents the results of a study on the effectiveness of vindeburnol, a structural analogue of the cerebral vasodilator vincamine, in the model of multiple sclerosis. The drug was injected subcutaneously in rats after modeling experimental allergic encephalomyelitis at a dose of 20 μg/kg, from the first manifestation of a neurological deficit over the next 10 days. In the somatosensory cortex of animals, the activity of superoxide dismutase, glutathione peroxidase and glutathione reductase, as well as the expression level of the SOD1, GPX4, GPX6 and GSR genes using real-time polymerase chain reaction were studied. It was found that vindeburnol does not affect the expression of the SOD1 gene, as well as the activity of superoxide dismutase, while increasing the expression of the GPS4, GPS6 and GRS genes, which determines the increased activity of glutathione peroxidase and glutathione reductase in rats in the model of multiple sclerosis.

scholarly journals Effect of Cross-Sex Hormonal Replacement on Antioxidant Enzymes in Rat Retroperitoneal Fat Adipocytes

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Israel Pérez-Torres ◽  
Verónica Guarner-Lans ◽  
Alejandra Zúñiga-Muñoz ◽  
Rodrigo Velázquez Espejel ◽  
Alfredo Cabrera-Orefice ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Enzymatic Activities ◽  
Glutathione S Transferase ◽  
Control Groups ◽  
Intact Female ◽  
Hormonal Replacement

We report the effect of cross-sex hormonal replacement on antioxidant enzymes from rat retroperitoneal fat adipocytes. Eight rats of each gender were assigned to each of the following groups: control groups were intact female or male (F and M, resp.). Experimental groups were ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone (OvxF + T), and CasM plus estradiol (CasM + E2) groups. After sacrifice, retroperitoneal fat was dissected and processed for histology. Adipocytes were isolated and the following enzymatic activities were determined: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). Also, glutathione (GSH) and lipid peroxidation (LPO) were measured. In OvxF, retroperitoneal fat increased and adipocytes were enlarged, while in CasM rats a decrease in retroperitoneal fat and small adipocytes are observed. The cross-sex hormonal replacement in F rats was associated with larger adipocytes and a further decreased activity of Cu-Zn SOD, CAT, GPx, GST, GR, and GSH, in addition to an increase in LPO. CasM + E2exhibited the opposite effects showing further activation antioxidant enzymes and decreases in LPO. In conclusion, E2deficiency favors an increase in retroperitoneal fat and large adipocytes. Cross-sex hormonal replacement in F rats aggravates the condition by inhibiting antioxidant enzymes.

Detection of Herpesviridae in postmortem multiple sclerosis brain tissue and controls by polymerase chain reaction

1996 ◽  
Vol 2 (4) ◽  
pp. 249-258 ◽  
Author(s):  
Virginia J Sanders ◽  
Stephen Felisan ◽  
Aimee Waddell ◽  
Wallace W Tourtellotte
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Brain Tissue ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Intensity of cardiac free-radicals processes and expression of glutathione peroxidase and glutathione reductase genes in rats with adrenaline

2012 ◽  
Vol 58 (5) ◽  
pp. 530-538 ◽  
Author(s):  
I.Y. Iskusnykh ◽  
T.N. Popova ◽  
O.S. Musharova
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Free Radical Oxidation ◽  
Pathological Process ◽  
Mrna Levels ◽  
Maximal Increase ◽  
Radical Oxidation ◽  
Polymerase Chain ◽  
Radical Processes

The correlation between changes in activities of glutathione peroxidase and glutathione reductase in heart of rats during development of adrenaline myocarditis and intensity of free radical processes estimated by biochemiluminesce parameters and the content of lipoperoxidation products was demonstrated. The maximal increase of glutathione peroxidase and glutathione reductase activities (in 1.8 and 1.4 times accordingly) was observed t 24 h after the development of the pathological process; this coincided with the maximum intensity of prosesses of free radical oxidation. Using combination of reverse transcriptions with real-time polymerase chain reaction the cardiac mRNA levels of glutathione peroxidase and glutathione reductase genes were determined during the development of adrenaline myocarditis in rats. Analysis of expression of glutathione peroxidase and glutathione reductase genes showed, that the level of this transcripts demonstrated 2,8- and 7,3- increase in rats with adrenaline myocarditis, respectively. Obviously, overexpression of these enzymes can increase the resistance of cardiomyocites to oxidative stress.

scholarly journals Comparison of oxidant and antioxidant status of seminal plasma and spermatozoa of several fish species

2013 ◽  
Vol 58 (No. 7) ◽  
pp. 313-320 ◽  
Author(s):  
A. Shaliutina-Kolešová ◽  
I. Gazo ◽  
J. Cosson ◽  
O. Linhart
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Seminal Plasma ◽  
Brook Trout ◽  
Siberian Sturgeon ◽  
Russian Sturgeon ◽  
Thiobarbituric Acid Reactive Substance ◽  
Acipenser Baerii ◽  
Carbonyl Protein

Oxidant and antioxidant activity in seminal plasma was compared with that in spermatozoa of teleost (common carp Cyprinus carpio and brook trout Salvelinus fontinalis) and chondrostean (Russian sturgeon Acipenser gueldenstaedtii, Siberian sturgeon Acipenser baerii, and sterlet Acipenser ruthenus) fishes. No differences were found between seminal plasma and spermatozoa in the level of thiobarbituric-acid-reactive substance (0.24 ± 0.08 to 0.33 ± 0.04 nmol/mg proteins) in Russian sturgeon, Siberian sturgeon, and sterlet. Carbonyl protein concentration was significantly higher in spermatozoa than in seminal plasma of all studied species. Analyzed antioxidants included superoxide dismutase, glutathione reductase, and glutathione peroxidase activity. Significant differences (P < 0.05) were detected between seminal plasma and spermatozoa in total superoxide dismutase (SOD) and glutathione reductase (GR). Total glutathione peroxidase (GPx) activity was significantly higher in brook trout (12.56 ± 3.23 mU/mg proteins) and Russian sturgeon (11.56 ± 3.12 mU/mg proteins) spermatozoa compared to seminal plasma (6.81 ± 1.56 mU/mg proteins in brook trout and 9.56 ± 3.12 mU/mg proteins in Russian sturgeon). This study provides new data on oxidant and antioxidant balance between spermatozoa and seminal plasma that may be of value in the development of methods for artificial reproduction of teleost and chondrostean species.  

Failure to Detect Measles Virus Rna, by Reverse Transcription-Polymerase Chain Reaction, in Peripheral Blood Leucocytes of Patients with Multiple Sclerosis

1996 ◽  
Vol 1 (4) ◽  
pp. 204-206 ◽  
Author(s):  
B. Brankin ◽  
M. Osman ◽  
L. Herlihy ◽  
S.A. Hawkins ◽  
S.L. Cosby
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Peripheral Blood ◽  
Measles Virus ◽  
Chain Reaction ◽  
Specific Primers ◽  
Polymerase Chain ◽  
Multiple Sclerosis Patients ◽  
Peripheral Blood Leucocytes

We have examined peripheral blood leucocytes (PBLs) from 17 multiple sclerosis patients, two patients with rheumatoid arthritis, one case of acute childhood measles and one case of subacute sclerosing panencephalitis, as well as 19 healthy adult controls for measles virus (MV) RNA, by the technique of reverse transcription-polymerase chain reaction. MV nucleocapsid gene specific primers were used to amplify all PBL-derived cDNA samples. These proved to be negative with the exception of the sample derived from the acute measles case. Selected cases were examined further, using fusion gene and matrix gene specific primers. MV RNA could not be detected.

scholarly journals Frequency of HLA-DRB1 Gene Alleles in Patients With Multiple Sclerosis in a Lithuanian Population

Medicina ◽  
2012 ◽  
Vol 48 (1) ◽  
pp. 2
Author(s):  
Renata Balnytė ◽  
Daiva Rastenytė ◽  
Dalia Mickevičienė ◽  
Antanas Vaitkus ◽  
Erika Skrodenienė ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Case Control Study ◽  
Case Control ◽  
Chain Reaction ◽  
Female Patients ◽  
History Of ◽  
Male Patients ◽  
Polymerase Chain ◽  
The Relationship ◽  
Control Study

The aim of the present study was to investigate the influence of HLA-DRB1 alleles on the genetic susceptibility to multiple sclerosis in the Lithuanian population. Material and Methods. A total of 120 patients with multiple sclerosis and 120 unrelated healthy controls were enrolled in this case-control study. Allelic frequencies were compared between the groups. HLA-DRB1 alleles were genotyped using the polymerase chain reaction. Results. HLA-DRB1*15 was present in 55.8% of the patients with multiple sclerosis and 10.0% of the controls (OR, 5.58; 95% CI, 3.19–9.77; P<0.0001). The protective alleles that were found to be more prevalent among the controls compared with the patients with multiple sclerosis were HLADRB1* 01 (26.7% vs. 7.5%, P<0.0001), *03 (17.5% vs. 8.3%, P=0.034), and *16 (11.7% vs. 3.3%, P=0.014). HLA-DRB1*15 was more common among the female patients with multiple sclerosis than among the male patients (68.4% vs. 34.1%; OR, 4.18; 95%, CI 1.90–9.22; P=0.001). The heterozygous inheritance of HLA-DRB1*15 allele was more common in the patients with a history of maternal multiple sclerosis than in those with a history of paternal multiple sclerosis (29.4% vs. 9.8%; P=0.045). Conclusions. HLA-DRB1*15 was found to be associated with multiple sclerosis in the Lithuanian population. This allele was more prevalent among the female patients with multiple sclerosis. Maternal multiple sclerosis was more common than paternal multiple sclerosis, but the relationship with HLA-DRB1*15 allele was not established. HLA-DRB1*01, *03, and *16 appeared to be the protective alleles in this series.

Dietary supplementation with magnesium citrate may improve pancreatic metabolic indices in an alloxan-induced diabetes rat model

10.5219/1375 ◽  
2020 ◽  
Vol 14 ◽  
pp. 836-846
Author(s):  
Olena Shatynska ◽  
Oleksandr Tokarskyy ◽  
Petro Lykhatskyi ◽  
Olha Yaremchuk ◽  
Iryna Bandas ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Fasting Glucose ◽  
Reduced Glutathione ◽  
Pancreatic Tissue ◽  
Magnesium Supplementation ◽  
Metabolism Enzymes ◽  
Peroxidation Index

The purpose of the current study was to evaluate the protective properties of dietary magnesium supplementation on pancreatic tissue of rats with alloxan-induced diabetes mellitus. Twenty-five male Wistar rats were split into five groups (control, diabetes, diabetes with 100 mg Mg daily, diabetes with 250 mg Mg daily, diabetes with 500 mg Mg daily) with feeding supplementation starting on day 1, diabetes induction on day 21, and animal sacrifice on day 30. Fasting glucose in blood serum was measured on days 21, 25, 27, and day 30. Glucose metabolism enzymes, namely, lactate dehydrogenase and glucose-6-phosphate dehydrogenase, were measured in pancreatic tissue upon the sacrifice, as well as lipid peroxidation, antioxidant system protective enzymes (catalase and superoxide dismutase), and glutathione system components (glutathione reductase, glutathione peroxidase, and glutathione reduced). Pearson correlation coefficients showed strong negative correlation between serum glucose (control and diabetic animals) and glucose metabolism enzymes, catalase, superoxide dismutase, glutathione peroxidase in pancreatic tissue (r >-0.9, p <0.05), moderate negative correlation with reduced glutathione (r = -0.79, p <0.05), moderate positive correlation with lipid peroxidation index (r = +0.67, p <0.05), weak correlation with glutathione reductase (r = -0.57, p <0.05). Magnesium supplementation slowed down diabetes onset considering fasting glucose levels in rats (p <0.05), as well as partially restored investigated dehydrogenase levels in the pancreas of rats comparing to diabetes group (p <0.05). The lipid peroxidation index varied between treatments showing the dose-dependent influence of Mg2+. Magnesium supplementation partially restored catalase and superoxide dismutase activities in pancreatic tissue, as well as glutathione peroxidase and reduced glutathione levels (p <0.05), while glutathione reductase levels remained unaffected (p >0.05). The obtained results suggested a model, where magnesium ions may have a possible protective effect on pancreatic tissue against the negative influence of alloxan inside β cells of the pancreas.

Activity of Superoxide Dismutase, Catalase, Glutathione Peroxidase, and Glutathione Reductase in Different Stages of Colorectal Carcinoma

2013 ◽  
Vol 58 (9) ◽  
pp. 2646-2652 ◽  
Author(s):  
Kristina R. Gopčević ◽  
Branislav R. Rovčanin ◽  
Svetislav B. Tatić ◽  
Zoran V. Krivokapić ◽  
Milan M. Gajić ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Colorectal Carcinoma ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase
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