Laser Actuated Non-Invasive Smart Instrumentation - Enabling  Lab-on-Chip

Cell mechanics is a discipline that bridges cell biology with mechanics. Emerging microscale technologies are opening new venues in the field, due to their costeffectiveness, relatively easy fabrication, and high throughput. Two examples of those technologies are discussed here: microfluidic impedance cytometry and erythrocyte electrodeformation. The former is a lab-on-chip technology offering a simple, non-invasive, label-free method for counting, identifying and monitoring cellular biophysical and mechanical function at the single-cell level. The latter is a useful complement to the former, enabling cell deformation under the influence of an applied electric field.

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Trapping and Optomechanical Sensing of Particles with a Nanobeam Photonic Crystal Cavity

Crystals ◽

10.3390/cryst9020057 ◽

2019 ◽

Vol 9 (2) ◽

pp. 57 ◽

Cited By ~ 2

Author(s):

Lin Ren ◽

Yunpeng Li ◽

Na Li ◽

Chao Chen

Keyword(s):

Photonic Crystal ◽

Optical Power ◽

Optical Force ◽

Aqueous Environment ◽

Particle Trapping ◽

Lab On Chip ◽

Photonic Crystal Cavity ◽

Non Invasive ◽

On Chip ◽

Functional Components

Particle trapping and sensing serve as important tools for non-invasive studies of individual molecule or cell in bio-photonics. For such applications, it is required that the optical power to trap and detect particles is as low as possible, since large optical power would have side effects on biological particles. In this work, we proposed to deploy a nanobeam photonic crystal cavity for particle trapping and opto-mechanical sensing. For particles captured at 300 K, the input optical power was predicted to be as low as 48.8 μW by calculating the optical force and potential of a polystyrene particle with a radius of 150 nm when the trapping cavity was set in an aqueous environment. Moreover, both the optical and mechanical frequency shifts for particles with different sizes were calculated, which can be detected and distinguished by the optomechanical coupling between the particle and the designed cavity. The relative variation of the mechanical frequency achieved approximately 400%, which indicated better particle sensing compared with the variation of the optical frequency (±0.06%). Therefore, our proposed cavity shows promising potential as functional components in future particle trapping and manipulating applications in lab-on-chip.

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A Bio-Manipulation Method Based on the Hydrodynamic Force of Multiple Microfluidic Streams

Journal of Robotics and Mechatronics ◽

10.20965/jrm.2013.p0611 ◽

2013 ◽

Vol 25 (4) ◽

pp. 611-618 ◽

Cited By ~ 6

Author(s):

Yaxiaer Yalikun ◽

◽

Yoshitake Akiyama ◽

Takayuki Hoshino ◽

Keisuke Morishima

Keyword(s):

Open Space ◽

Cfd Simulation ◽

Rectilinear Motion ◽

Lab On Chip ◽

Micro Assembly ◽

Multi Scale ◽

Non Invasive ◽

3D Manipulation ◽

On Chip ◽

Calculated Velocity

This paper proposes a multiple microfluidic streambased manipulation (MMSM) system for bio-objects. It uses micro hydrodynamics and lab on chip (LOC) technology. Our method can implement the functions of micro manipulation and micro assembly of bio-objects in an open space without contact. Compared to other conventional bio-micro-manipulation and assembly methods, this system manipulates micro objects by controlling multiple microfluidic streams onto them from various directions. The advantages of this method are that it performs open space, multifunction, multi-scale, multi-degree-of-freedom, and non-invasive 3D manipulation. These microfluidic streams are generated simultaneously from multiple orifices. By regulating the parameters of the microfluidic stream, such as the position and number of operating orifices and the flow rate, the direction and velocity of the object can be controlled. To verify this principle, we design an open-space fluidic system for on-chip manipulation and calculated velocity and direction of the microfluidic stream using CFD simulation. Then the prototype microchip with an array of nine orifices is fabricated from glass. In experiments, demonstrations of rectilinear motion of a single cell andmicro particle are observed. The results presented in this paper show that this MMSM is capable of biomicromanipulation and micro assembly of bio-objects.

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The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review

Veterinární Medicína ◽

10.17221/5913-vetmed ◽

2012 ◽

Vol 57 (No. 4) ◽

pp. 169-176 ◽

Cited By ~ 2

Author(s):

D. Bukowska ◽

B. Kempisty ◽

H. Piotrowska ◽

R. Walczak ◽

P. Sniadek ◽

...

Keyword(s):

Quality Assessment ◽

Embryo Quality ◽

Developmental Potential ◽

Vitro Fertilization ◽

Lab On Chip ◽

New Device ◽

Non Invasive ◽

Mammalian Oocyte ◽

On Chip

 The quality of oocytes-embryos can be determined by several techniques, including morphological, molecular, cellular and biochemical ones. The morphological methods of female gamete or embryo quality assessment often use thе following in vitro manipulation procedures such as: in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro embryo production (IVP). However, these methods are highly subjective and the morphological classification of oocytes or embryos is not always compatible with their ability to grow and develop. Additionally, molecular biology methods are objective and present parametric results, which are more or less comparable to the real oocyte-embryo “health”. Although these techniques enable us to determine markers of oocyte-embryo developmental potential, when applied they lead to destruction of the analysed cells. Therefore, the need still exists to search for new methods that will be highly objective (parametric) and, which is most important, non-invasive. In this review, the morphological and molecular methods of oocyte-embryo quality assessment are presented. Moreover, we described a new system based on microfluidic technology (Lab-on-Chip) which allows the creation of a new device for mammalian oocyte as well as embryo quality evaluation: by using their spectral characterisation following embryo transfer (ET) procedures in the cattle and the pig.    

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Developmental competence of mammalian oocytes-insights into molecular research and the promise of microfluidic technology: a review

Veterinární Medicína ◽

10.17221/8580-vetmed ◽

2017 ◽

Vol 60 (No. 12) ◽

pp. 663-674 ◽

Cited By ~ 1

Author(s):

B. Kempisty ◽

P. Zawierucha ◽

S. Ciesiolka ◽

H. Piotrowska ◽

P. Antosik ◽

...

Keyword(s):

Oocyte Quality ◽

Developmental Competence ◽

Extrinsic Factors ◽

Gene Markers ◽

Lab On Chip ◽

Non Invasive ◽

Oocyte Developmental Competence ◽

Microfluidic Technology ◽

Follicular Size ◽

On Chip

Developmental competence of female gametes determines their maturation ability, successful fertilisation, and proper zygote formation. Oocyte quality may be assessed by expression profiling of several gene markers such as Cx43, TGFB, GDF9, BMP, Lox and Pdia5 that determine the biological features of oocytes. Conversely, several other extrinsic factors, including follicular size or heat shock may significantly influence oocyte quality and ability to grow and develop during folliculo- and oogenesis. However, using molecular methods for evaluation of oocyte quality often leads to destruction of an analysed cell. Therefore, there is an increased requirement to seek new non-invasive methods of oocyte-embryo quality assessment. Here we describe the Lab-on-Chip system based on microfluidic technology, which is the first parametric and objective device for evaluation of oocyte developmental competence using spectral images. In this review several extrinsic factors and molecular markers of oocyte developmental competence are discussed. Furthermore, based on our previous studies, we discuss the possibility of applying the spectrophotometric system (Lab-on-Chip) in both biomedical and reproductive research in domestic animals.

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Salt Precipitation During Injection of CO2 into Saline Aquifers: Lab-on-Chip Experiments on Glass and Geomaterial Microfluidic Specimens

SSRN Electronic Journal ◽

10.2139/ssrn.3365553 ◽

2019 ◽

Author(s):

Mohammad Nooraiepour ◽

Hossein Fazeli ◽

Rohaldin Miri ◽

Helge Hellevang

Keyword(s):

Saline Aquifers ◽

Salt Precipitation ◽

Lab On Chip ◽

On Chip

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A Lab‐On‐chip Tool for Rapid, Quantitative, and Stage‐selective Diagnosis of Malaria

Advanced Science ◽

10.1002/advs.202004101 ◽

2021 ◽

pp. 2004101

Author(s):

Marco Giacometti ◽

Francesca Milesi ◽

Pietro Lorenzo Coppadoro ◽

Alberto Rizzo ◽

Federico Fagiani ◽

...

Keyword(s):

Lab On Chip ◽

On Chip

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Integrated Magnetohydrodynamic Pump with Magnetic Composite Substrate and Laser-Induced Graphene Electrodes

Polymers ◽

10.3390/polym13071113 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1113

Author(s):

Mohammed Asadullah Khan ◽

Jürgen Kosel

Keyword(s):

Magnetic Flux ◽

Flow Rate ◽

Magnetic Composite ◽

Propulsive Force ◽

Closed Channel ◽

Lab On Chip ◽

Composite Substrate ◽

On Chip ◽

High Level ◽

Moving Parts

An integrated polymer-based magnetohydrodynamic (MHD) pump that can actuate saline fluids in closed-channel devices is presented. MHD pumps are attractive for lab-on-chip applications, due to their ability to provide high propulsive force without any moving parts. Unlike other MHD devices, a high level of integration is demonstrated by incorporating both laser-induced graphene (LIG) electrodes as well as a NdFeB magnetic-flux source in the NdFeB-polydimethylsiloxane permanent magnetic composite substrate. The effects of transferring the LIG film from polyimide to the magnetic composite substrate were studied. Operation of the integrated magneto hydrodynamic pump without disruptive bubbles was achieved. In the studied case, the pump produces a flow rate of 28.1 µL/min. while consuming ~1 mW power.

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Towards lab-on-chip ultrasensitive ethanol detection using photonic crystal waveguide operating in the mid infrared

Nanophotonics ◽

10.1515/nanoph-2020-0576 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Ali Rostamian ◽

Ehsan Madadi-Kandjani ◽

Hamed Dalir ◽

Volker J. Sorger ◽

Ray T. Chen

Keyword(s):

Photonic Crystal ◽

Slow Light ◽

High Sensitivity ◽

Guided Wave ◽

Silicon On Insulator ◽

Group Index ◽

Photonic Crystal Waveguide ◽

Mid Infrared ◽

Lab On Chip ◽

On Chip

Abstract Thanks to the unique molecular fingerprints in the mid-infrared spectral region, absorption spectroscopy in this regime has attracted widespread attention in recent years. Contrary to commercially available infrared spectrometers, which are limited by being bulky and cost-intensive, laboratory-on-chip infrared spectrometers can offer sensor advancements including raw sensing performance in addition to use such as enhanced portability. Several platforms have been proposed in the past for on-chip ethanol detection. However, selective sensing with high sensitivity at room temperature has remained a challenge. Here, we experimentally demonstrate an on-chip ethyl alcohol sensor based on a holey photonic crystal waveguide on silicon on insulator-based photonics sensing platform offering an enhanced photoabsorption thus improving sensitivity. This is achieved by designing and engineering an optical slow-light mode with a high group-index of n g = 73 and a strong localization of modal power in analyte, enabled by the photonic crystal waveguide structure. This approach includes a codesign paradigm that uniquely features an increased effective path length traversed by the guided wave through the to-be-sensed gas analyte. This PIC-based lab-on-chip sensor is exemplary, spectrally designed to operate at the center wavelength of 3.4 μm to match the peak absorbance for ethanol. However, the slow-light enhancement concept is universal offering to cover a wide design-window and spectral ranges towards sensing a plurality of gas species. Using the holey photonic crystal waveguide, we demonstrate the capability of achieving parts per billion levels of gas detection precision. High sensitivity combined with tailorable spectral range along with a compact form-factor enables a new class of portable photonic sensor platforms when combined with integrated with quantum cascade laser and detectors.

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