Role of the products of oocyte and embryo metabolism in patients with external genital endometriosis in successful embryo implantation

2020 ◽  
Vol 4_2020 ◽  
pp. 21-25
Author(s):  
Smolnikova V.Yu. Smolnikova ◽  
Ibragimova L.K. Ibragimova ◽  
Bobrov M.Yu. Bobrov ◽  
Romanov E.A. Romanov ◽  
Keyword(s):  
Embryo Implantation ◽  
Embryo Metabolism

scholarly journals Role of Vascular Endothelial Growth Factor (VEGF) in Human Embryo Implantation: Clinical Implications

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 253
Author(s):  
Xi Guo ◽  
Hong Yi ◽  
Tin Chiu Li ◽  
Yu Wang ◽  
Huilin Wang ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Recurrent Miscarriage ◽  
Embryo Implantation ◽  
Critical Role ◽  
Angiogenic Factor ◽  
Vascular Endothelial ◽  
Underlying Mechanisms ◽  
Endothelial Growth Factor

Vascular endothelial growth factor (VEGF) is a well-known angiogenic factor that plays a critical role in various physiological and pathological processes. VEGF also contributes to the process of embryo implantation by enhancing embryo development, improving endometrial receptivity, and facilitating the interactions between the developing embryo and the endometrium. There is a correlation between the alteration of VEGF expression and reproductive failure, including recurrent implantation failure (RIF) and recurrent miscarriage (RM). In order to clarify the role of VEGF in embryo implantation, we reviewed recent literature concerning the expression and function of VEGF in the reproductive system around the time of embryo implantation and we provide a summary of the findings reported so far. We also explored the effects and the possible underlying mechanisms of action of VEGF in embryo implantation.

scholarly journals COUP-TFII Regulates Human Endometrial Stromal Genes Involved in Inflammation

2013 ◽  
Vol 27 (12) ◽  
pp. 2041-2054 ◽  
Author(s):  
Xilong Li ◽  
Michael J. Large ◽  
Chad J. Creighton ◽  
Rainer B. Lanz ◽  
Jae-Wook Jeong ◽  
...  
Keyword(s):  
Cell Fate ◽  
Cell Biology ◽  
Embryo Implantation ◽  
Orphan Nuclear Receptor ◽  
Loss Of Function ◽  
Mouse Uterus ◽  
Endometrial Stromal Cells ◽  
Endometrial Stroma ◽  
Stroma Cell

Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII; NR2F2) is an orphan nuclear receptor involved in cell-fate specification, organogenesis, angiogenesis, and metabolism. Ablation of COUP-TFII in the mouse uterus causes infertility due to defects in embryo attachment and impaired uterine stromal cell decidualization. Although the function of COUP-TFII in uterine decidualization has been described in mice, its role in the human uterus remains unknown. We observed that, as in mice, COUP-TFII is robustly expressed in the endometrial stroma of healthy women, and its expression is reduced in the ectopic lesions of women with endometriosis. To interrogate the role of COUP-TFII in human endometrial function, we used a small interfering RNA-mediated loss of function approach in primary human endometrial stromal cells. Attenuation of COUP-TFII expression did not completely block decidualization; rather it had a selective effect on gene expression. To better elucidate the role of COUP-TFII in endometrial stroma cell biology, the COUP-TFII transcriptome was defined by pairing microarray comparison with chromatin immunoprecipitation followed by deep sequencing. Gene ontology analysis demonstrates that COUP-TFII regulates a subset of genes in endometrial stroma cell decidualization such as those involved in cell adhesion, angiogenesis, and inflammation. Importantly this analysis shows that COUP-TFII plays a role in controlling the expression of inflammatory cytokines. The determination that COUP-TFII plays a role in inflammation may add insight into the role of COUP-TFII in embryo implantation and in endometrial diseases such as endometriosis.

The role of the leukemia inhibitory factor receptor in embryo implantation and placentation

Placenta ◽  
2021 ◽  
Vol 114 ◽  
pp. 139
Author(s):  
Jumpei Terakawa ◽  
Kazuhiro Matsuo ◽  
Takafumi Namiki ◽  
Kana Ohtomo ◽  
Atsuko Kageyama ◽  
...  
Keyword(s):  
Leukemia Inhibitory Factor ◽  
Embryo Implantation ◽  
Inhibitory Factor ◽  
Leukemia Inhibitory Factor Receptor

scholarly journals Microbiota and Human Reproduction: The Case of Female Infertility

2020 ◽  
Vol 9 (2) ◽  
pp. 12 ◽  
Author(s):  
Rossella Tomaiuolo ◽  
Iolanda Veneruso ◽  
Federica Cariati ◽  
Valeria D’Argenio
Keyword(s):  
Reproductive Tract ◽  
Assisted Reproductive Technologies ◽  
Embryo Implantation ◽  
Reproductive Technologies ◽  
Human Reproduction ◽  
Microbial Dysbiosis ◽  
Technical Issues ◽  
Almost All ◽  
Generation Sequencing

During the last decade, the availability of next-generation sequencing-based approaches has revealed the presence of microbial communities in almost all the human body, including the reproductive tract. As for other body sites, this resident microbiota has been involved in the maintenance of a healthy status. As a consequence, alterations due to internal or external factors may lead to microbial dysbiosis and to the development of pathologies. Female reproductive microbiota has also been suggested to affect infertility, and it may play a key role in the success of assisted reproductive technologies, such as embryo implantation and pregnancy care. While the vaginal microbiota is well described, the uterine microbiota is underexplored. This could be due to technical issues, as the uterus is a low biomass environment. Here, we review the state of the art regarding the role of the female reproductive system microbiota in women’s health and human reproduction, highlighting its contribution to infertility.

scholarly journals PARP1 during embryo implantation and its upregulation by oestradiol in mice

Reproduction ◽  
2014 ◽  
Vol 147 (6) ◽  
pp. 765-780 ◽  
Author(s):  
Anubha Joshi ◽  
Sahil Mahfooz ◽  
Vineet Kumar Maurya ◽  
Vijay Kumar ◽  
Chadchan Sangappa Basanna ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Embryo Implantation ◽  
Transcript Level ◽  
Tissue Remodelling ◽  
Native Form ◽  
Ovarian Steroids ◽  
Blastocyst Implantation ◽  
Implantation Sites ◽  
Implantation Period

Pregnancy requires successful implantation of an embryo, which occurs during a restricted period defined as ‘receptivity of the endometrium’ and is influenced by the ovarian steroids progesterone and oestradiol. The role of poly(ADP-ribose)polymerase-1 (PARP1) in apoptosis is well established. However, it is also involved in cell differentiation, proliferation and tissue remodelling. Previous studies have described the presence of PARP in the uterus, but its exact role in embryo implantation is not yet elucidated. Hence, in this study, we studied the expression of PARP1 in the uterus during embryo implantation and decidualisation, and its regulation by ovarian steroids. Our results show upregulation of the native form of PARP1 (∼116 kDa) in the cytosolic and nuclear compartments of implantation and non-implantation sites at day 5 (0500 h), followed by downregulation at day 5 (1000 h), during the embryo implantation period. The transcript level of Parp1 was also augmented during day 5 (0500 h). Inhibition of PARP1 activity by the drug EB-47 decreased the number of embryo implantation sites and blastocysts at day 5 (1000 h). Further, cleavage of native PARP1 was due to the activity of caspase-3 during the peri-implantation stage (day 5 (0500 h)), and is also required for embryo implantation, as inhibition of its activity compromised blastocyst implantation. The native (∼116 kDa) and cleaved (∼89 kDa) forms of PARP1 were both elevated during decidualisation of the uterus. Furthermore, the expression level of PARP1 in the uterus was found to be under the control of the hormone oestrogen. Our results clearly demonstrate that PARP1 participates in the process of embryo implantation.

scholarly journals Leptin Upregulates the Expression of β3-Integrin, MMP9, HB-EGF, and IL-1β in Primary Porcine Endometrium Epithelial Cells In Vitro

2020 ◽  
Vol 17 (18) ◽  
pp. 6508
Author(s):  
Hongfang Wang ◽  
Jinlian Fu ◽  
Aiguo Wang
Keyword(s):  
Signaling Pathways ◽  
Embryo Implantation ◽  
Mapk Signaling ◽  
Dependent Manner ◽  
Reproductive Disorders ◽  
Β3 Integrin ◽  
Epithelium Cells ◽  
Dose Dependent

Obesity has become a global health problem. Research suggests that leptin, a hormone that responds to fat deposition, may be involved in mammalian reproduction; however, its precise role in embryo implantation is poorly understood. Here, primary porcine endometrium epithelium cells (PEECs) were cultured in vitro and used to evaluate the regulatory role of different leptin levels on β3-integrin, MMP9, HB-EGF, and IL-1β, which are, respectively, involved in four critical steps of embryo implantation. Results showed that only 0.01 nM leptin significantly improved β3-integrin mRNA expression (p < 0.05). MMP9 and HB-EGF mRNA expressions were upregulated by 0.10–10.00 nM leptin (p < 0.05). The IL-1β expression level was only increased by 10.00 nM leptin (p < 0.05). β3-integrin, MMP9, HB-EGF, and IL-1β mRNA and protein have a similar fluctuant response to increased leptin. Leptin’s influence on β3-integrin, MMP9, HB-EGF, and IL-1β disappeared when the JAK2, PI(3)K, or MAPK signaling pathways were blocked, respectively. In conclusion, leptin affected porcine implantation by regulating the expression of β3-integrin, MMP9, HB-EGF, and IL-1β in a dose-dependent manner. The signaling pathways of JAK2, PI(3)K, and MAPK may participate in this regulatory process. These findings will contribute to further understanding the mechanisms of reproductive disorders in obesity.

The role of progesterone induced blocking factor (PIBF) in murine embryo implantation

2016 ◽  
Vol 115 ◽  
pp. 88
Author(s):  
Tímea J. Csabai ◽  
Zoltán Bognár ◽  
Júlia Szekeres-Barthó
Keyword(s):  
Embryo Implantation ◽  
Blocking Factor ◽  
Murine Embryo
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