The role of the folate cycle genes in development of uterine fibroids

Low provision of cells with vitamin B2 and magnesium leads to a decrease in the activity of the sirtuin-1 deacetylase and an increase in the activity of the pro-inflammatory factor NF-kB, a decrease in the levels of glutathione, an increase in the levels of homocysteine, thrombus formation, the activity of mitochondria, the development of migraine, convulsions and miscarriage. The role of riboflavin in the regulation of the folate cycle in the genotype MTHFR 677TT for the prevention of folatresistant fetal malformations, the advantages of an aqueous solution of riboflavin and magnesium citrate is considered. The data on titanium dioxide, which increases the level of pro-inflammatory cytokines IL-1b, IL-4, IL-5, IL-6, G-CSF, CCL-2, CCL-3, CCL-4, are presented.

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Role of Laparoscopy in Infertility : Review Article

BIRDEM Medical Journal ◽

10.3329/birdem.v2i2.12324 ◽

2012 ◽

Vol 2 (2) ◽

pp. 99-103 ◽

Cited By ~ 1

Author(s):

S Jahan

Keyword(s):

Fiber Optics ◽

Semen Analysis ◽

Uterine Fibroids ◽

Scar Tissue ◽

Direct Visualization ◽

Male Factor ◽

Operative Laparoscopy ◽

Pelvic Organs ◽

Carbon Dioxide Co2

Infertility is defined as failure to conceive during one year of unprotected frequent intercourse. Leading causes of infertility include tubal disease, ovulatory disorders, uterine or cervical factors, endometriosis and male factor infertility. A laparoscope is a thin fiber optic telescope that is inserted into the abdomen usually through the belly button. The fiber optics allow a light to used to see inside the abdomen. Carbon dioxide (CO2) gas is placed into the abdomen prior to inserting the laparoscope. Generally, laparoscopy should be reserved for couples who have already completed a more basic infertility evaluation including assessing for ovulation, ovarian reserve, ultrasound and hysterosalpingogram for the female and semen analysis for the male. Laparoscopy can help physicians diagnose many gynecological problems including endometriosis, uterine fibroids and other structural abnormalities, ovarian cysts, adhesions (scar tissue), and ectopic pregnancy. Robotic assisted laparoscopic surgery (RAL) is a more recent development and a form of operative laparoscopy. In RAL, the instruments and telescope are very similar to conventional laparoscopy, but they are attached to a robot which in turn is controlled by the surgeon who is seated at a viewing console. Women who have been diagnosed with endometriosis are more likely to experience infertility, and observational studies have shown that the monthly probability of pregnancy in women with endometriosis is about half of the probability in normal women. In spite of this well-documented association, a true cause and effect relationship has not been established. Laparoscopy is used world-wide to investigate infertility. It is an essential part of full assessment and treatment of infertility. It provides direct visualization of the pelvic organs, ovarian and tubal status and can elucidate the site of tubal obstruction. It has got an advantage of direct visualization of the pelvic organs and the peri-tubal status resulting in greater information as compared to hysterosalpingography and ultrasonography. The advance in instrument technology has made this procedure more productive and less hazardous. Laparoscopy is the most dependable tool to investigate pelvic pathology. The role of laparoscopy in diagnosis of infertility both primary as well as secondary is established beyond any doubt.DOI: http://dx.doi.org/10.3329/birdem.v2i2.12324 (Birdem Med J 2012; 2(2): 99-103)

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ROLE OF GENETIC POLYMORPHISMS ASSOCIATED WITH THROMBOPHILIA, PROTHROMBOTIC STATE AND FOLATE CYCLE DISTURBANCES IN WOMEN WITH REPRODUCTIVE DISORDERS

Тромбоз, гемостаз и реология ◽

10.25555/thr.2017.2.0784 ◽

2017 ◽

Author(s):

А. Киселева ◽

Е. Бутина ◽

Г. Зайцева ◽

Е. Попонина ◽

С. Игнатьев ◽

...

Keyword(s):

Genetic Disorders ◽

Folate Metabolism ◽

Clear Understanding ◽

Reproductive Disorders ◽

Primary Infertility ◽

Risk Alleles ◽

Heterozygous Form ◽

Folate Cycle ◽

Pai 1

Введение. Причины осложнения беременности достаточно многообразны: хромосомные и генетические нарушения, эндокринопатии, анатомические, инфекционные, иммунные и тромбофилические факторы. До настоящего времени нет четкого представления об истинных составляющих нарушения гестационного процесса. Материалы и методы. Проведено исследование аллелей, ассоциированных с тромбофилией и протромботическими состояниями, у 629 женщин и генов фолатного обмена у 277 женщин с отягощенным акушерским анамнезом. Результаты. Проанализирован характер распределения «аллелей риска» у женщин с первичным бесплодием, осложненными родами, неразвивающимися беременностями и у женщин без отягощенного акушерского анамнеза. Представлены данные о частоте встречаемости генетических мутаций, ассоциированных с тромбофилией (F2, F5), протромботическими состояниями (F7, F13, FGB, ITGA-2, ITGB-3, PAI-1) и нарушениями фолатного обмена (MTFFR, MTR, MTRR) у женщин с репродуктивными расстройствами. Заключение. Обнаружены статистически значимые различия в частоте распределения гетерозиготной формы полиморфизма ITGB3 у женщин с первичным бесплодием и осложненными родами в сравнении с женщинами без репродуктивных неудач. Влияние других полиморфных генов, ассоциированных с тромбофилией, протромботическими состояниями и нарушениями фолатного обмена, на наступление, течение и исход беременности не подтверждено. Introduction. The causes of pregnancy complications are quite multiformous: chromosomal and genetic disorders, endocrinopathies, anatomical, infectious, immune and thrombophilic factors. Until now there is no clear understanding of true components of gestational disorders. Materials and methods. We studied alleles associated with thrombophilia and prothrombotic conditions (in 629 women) and folate metabolism genes (in 277 women) with burdened obstetrical anamnesis. Results. The distribution of «risk alleles» in women with primary infertility, complicated delivery, non-developing pregnancy and at women without aggravated obstetric history was analyzed. Data about incidence of genetic mutations associated with thrombophilia (F2, F5), prothrombotic states (F7, F13, FGB, ITGA-2, ITGB-3, PAI-1) and folate metabolism disturbances (MTFFR, MTR, MTRR) in women with reproductive disorders are presented. Conclusion. Statistically signifi cant diff erences in distribution rate of heterozygous form of ITGB3 polymorphism in women with primary infertility and complicated delivery in comparison with women without reproductive failures were found. Impact of other polymorphic genes associated with thrombophilia, prothrombotic states and disturbances of folate metabolism to onset, gestation course and outcome of pregnancy was not proved.

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The role of clinical radiology in the management of uterine fibroids

British Journal of Hospital Medicine ◽

10.12968/hmed.2019.80.5.c66 ◽

2019 ◽

Vol 80 (5) ◽

pp. C66-C69

Author(s):

Shao J Ong ◽

Yen Zhi Tang ◽

Nadeem Shaida

Keyword(s):

Uterine Fibroids ◽

Clinical Radiology

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Overhydroxylation of Lysine of Collagen Increases Uterine Fibroids Proliferation: Roles of Lysyl Hydroxylases, Lysyl Oxidases, and Matrix Metalloproteinases

BioMed Research International ◽

10.1155/2017/5316845 ◽

2017 ◽

Vol 2017 ◽

pp. 1-13 ◽

Cited By ~ 6

Author(s):

Marwa Kamel ◽

Mohamed Wagih ◽

Gokhan S. Kilic ◽

Concepcion R. Diaz-Arrastia ◽

Mohamed A. Baraka ◽

...

Keyword(s):

Matrix Metalloproteinases ◽

Treatment Options ◽

Uterine Fibroids ◽

Cross Linking ◽

Ki 67 ◽

Subsequent Formation ◽

Lysyl Oxidases ◽

Cross Links ◽

Collagen Cross Linking

The role of the extracellular matrix (ECM) in uterine fibroids (UF) has recently been appreciated. Overhydroxylation of lysine residues and the subsequent formation of hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) cross-links underlie the ECM stiffness and profoundly affect tumor progression. The aim of the current study was to investigate the relationship between ECM of UF, collagen and collagen cross-linking enzymes [lysyl hydroxylases (LH) and lysyl oxidases (LOX)], and the development and progression of UF. Our results indicated that hydroxyl lysine (Hyl) and HP cross-links are significantly higher in UF compared to the normal myometrial tissues accompanied by increased expression of LH (LH2b) and LOX. Also, increased resistance to matrix metalloproteinases (MMP) proteolytic degradation activity was observed. Furthermore, the extent of collagen cross-links was positively correlated with the expression of myofibroblast marker (α-SMA), growth-promoting markers (PCNA; pERK1/2;FAKpY397; Ki-67; and Cyclin D1), and the size of UF. In conclusion, our study defines the role of overhydroxylation of collagen and collagen cross-linking enzymes in modulating UF cell proliferation, differentiation, and resistance to MMP. These effects can establish microenvironment conducive for UF progression and thus represent potential target treatment options of UF.

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The role of epidermal growth factor receptor (EGFR) common gene mutations in Iranian women with uterine fibroids

European Journal of Obstetrics & Gynecology and Reproductive Biology ◽

10.1016/j.ejogrb.2018.08.017 ◽

2018 ◽

Vol 229 ◽

pp. 103-107

Author(s):

Paria Nikpey ◽

Tahereh Nazari ◽

Shadi Khalili ◽

Ahmad Ebrahimi

Keyword(s):

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Uterine Fibroids ◽

Growth Factor Receptor ◽

Gene Mutations ◽

Iranian Women ◽

Common Gene ◽

Epidermal Growth

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Ulipristal acetate (UPA) for fibroids–IVF outcomes following treatment with UPA after IVF failure: series of 2 case reports

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20172959 ◽

2017 ◽

Vol 6 (7) ◽

pp. 3177 ◽

Cited By ~ 1

Author(s):

Ashish R Kale

Keyword(s):

Case Reports ◽

Uterine Fibroids ◽

Case Series ◽

Standard Of Care ◽

Infertile Woman ◽

Ulipristal Acetate ◽

Uterine Tumors ◽

Size Number ◽

Ivf Failure

Uterine fibroids (also known as leiomyomas or myomas) are the most common form of benign uterine tumors. Fibroids are present in 5-10% of infertile patients, and may be the sole cause of infertility in 1-2.4%. The removal of fibroids by hysteroscopy and laparoscopy has now become the standard of care depending upon the size, number and location of fibroids in an infertile woman. Off lately, there is growing evidence of the crucial role of progesterone pathways in the pathophysiology of uterine fibroids due to the use of selective progesterone receptor modulators (SPRMs) such as ulipristal acetate (UPA). Here, we report a case series of 2 women, with fibroids, who were put on UPA prior to IVF. These women had at least one previous IVF failure. The pre and post UPA fibroid characteristics were compared to see the effect of the drug. IVF was done post 3 month UPA therapy. Both the women showed a drastic reduction in the fibroid size, increased distance from endometrial cavity, and successful post UPA IVF cycles. Safety of the drug has been proven in few recent trials, but the role of UPA in infertile women with fibroids, who have had previous IVF failure, has opened new horizons in this field.

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