scholarly journals Determination of the molecular mass of hydrolyzed fibroin obtained from natural silk fibroin by spectrophotometry

2022 ◽  
pp. 115-120
Author(s):  
Khushnudbek ESHCHANOV ◽  
Muhabbat BALTAYEVA
Keyword(s):  
Molecular Mass ◽  
Silk Fibroin ◽  
Natural Silk

A Convenient Method for Molecular Mass Determination of Heparin

1992 ◽  
Vol 68 (04) ◽  
pp. 478-480 ◽  
Author(s):  
Jørgen Ilum Nielsen
Keyword(s):  
Molecular Mass ◽  
Convenient Method ◽  
Mass Determination ◽  
Molecular Mass Determination

New approaches for the determination of dextran in the sugar production process

2018 ◽  
pp. 138-146 ◽  
Author(s):  
Karin Abraham ◽  
Eckhard Flöter
Keyword(s):  
Molecular Mass ◽  
Mass Distribution ◽  
Molecular Mass Distribution ◽  
Molecular Size ◽  
Mass Variation ◽  
Negative Effects ◽  
New Approaches ◽  
Precise Knowledge ◽  
Enzyme Application

The presence of polysaccharides in cane and beet raw juices causes several negative effects during the sugar manufacture. These are usually mitigated by enzymatic decomposition of dextrans. Such effects not only depend on the content, but also on the molecular mass distribution. This means that the different dextran fractions specifically affect the process. An accurate process control hence requires the most precise knowledge about the existing content and the molecular mass distribution present. A detailed understanding of the specific processing problems and also a targeted enzyme application hence requires the determination of a total dextran content and also its characterization including the differentiation between the different dextran fractions. An accurate analytical tool which equally satisfies industrial applicability is still lacking. To improve on this situation, two new approaches for the determination of dextran were developed and benchmarked against the commonly used and established Haze Method, which is rather inaccurate and also sensitive to molecular mass variation. The two new approaches are both based on polarimetry. These two methods indicate to be superior over the Haze Method with respect two molecular mass variation and hence enable the determination of a broader molecular size range including also low molecular mass dextrans.

scholarly journals Determination of the molecular mass of apolipoprotein B-100 A chemical approach

1986 ◽  
Vol 239 (3) ◽  
pp. 777-780 ◽  
Author(s):  
C Y Yang ◽  
F S Lee ◽  
L Chan ◽  
D A Sparrow ◽  
J T Sparrow ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Apolipoprotein B ◽  
Cell Surface Receptor ◽  
Specific Cell ◽  
Apo B ◽  
Chemical Approach ◽  
Surface Receptor ◽  
Cnbr Cleavage ◽  
Specific Cell Surface

Apolipoprotein B-100 (apo B-100) is the protein ligand in low-density lipoproteins that binds to a specific cell-surface receptor. Its molecular mass has been a subject of controversy. We have determined the molecular mass of the protein by a chemical approach. After complete CNBr cleavage, the C-terminal fragment of apo B-100 was purified by reverse-phase h.p.l.c. Amino acid N- and C-terminal analyses confirm that this peptide represents the C-terminal peptide as deduced from the DNA sequence of a human apo B-100 cDNA clone. A chemically synthesized peptide was used to determine the recovery of the peptide (74.72%). On the basis of these data, the molecular mass of apo B-100 was determined to be 496.82 +/- 24.84 kDa.

Evaluation of “Solvent-Peak Separation” Column for the Determination of Polymer Molecular Mass Averages by SEC

1997 ◽  
Vol 4 (2) ◽  
pp. 87-97 ◽  
Author(s):  
Sadao Mori ◽  
Hélène Maréchal ◽  
Hiroshi Suzuki
Keyword(s):  
Molecular Mass ◽  
Peak Separation ◽  
Separation Column
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