scholarly journals Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro

2021 ◽  
pp. 22-29
Author(s):  
E. A. Kalashnikova ◽  
R. N. Kirakosyan ◽  
A. V. Gushchin ◽  
K. G. Abubakarov ◽  
N. N. Sleptsov ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Human Life ◽  
Red Light ◽  
The Body ◽  
Ex Vitro ◽  
High Quality ◽  
Planting Material ◽  
Micro Propagation

Relevance. Currently, food products that include prebiotics, in particular, inulin, are particularly popular. Interest in this substance is justified by its valuable properties – it is a good immunomodulator, cleanses the body of toxins, radionuclides, "bad" cholesterol, promotes the assimilation of useful trace elements necessary for human life. Inulin is contained in plants such as jerusalem artichoke, chicory, as well as in sweet potatoes, the popularity of which is increasing every year. However, sweet potato plants are afraid of cold and frost-resistant. Therefore, the creation of new varieties and hybrids that are resistant to low temperatures is an urgent problem. Cellular biotechnology is aimed at solving this problem using methods of clonal microreproduction, cell selection, somatic hybridization, etc. For rapid reproduction and obtaining high-quality planting material, biotechnology methods are used, in particular, clonal micro-propagation. However, in this technology there are difficulties associated with poor adaptation of microclones to ex vitro conditions. This fact introduces an additional requirement for the selection of optimal rooting modes in vitro and ex vitro adaptation of microclones.Material and methodology. The aim of the work was to study the influence of cultivation conditions on in vitro rooting and ex vitro adaptation of I. batatas (L.) microclones. The object of the study was sweet potato microgears propagated in vitro. I. batatas micro-gears were cultured in vitro on a Murashige-Skug medium, differing by the type of auxins. The influence of red (R) and far red (FR) light on the shoots rooting in vitro and the adaptation of microclones ex vitro was studied.Results. It has been experimentally established that the cultivation of micro-gears on a medium containing indolyl butyric acid at a concentration of 0.5-1 mg/l and under conditions of illumination by LED lamps of red and far red light in equal amounts leads to the production of microclones with a well-developed root system and vegetative biomass. The use of an aeroponic installation at the last stage of clonal micro-propagation makes it possible to obtain high-quality planting material that can adapt well to open ground conditions.

scholarly journals USING NATURAL LIGHT ON MICROPROPAGATION OF SWEET POTATO ( Ipomoea batatas L. ) IN AREA OF HO CHI MINH CITY

2012 ◽  
Vol 15 (3) ◽  
pp. 36-46
Author(s):  
Giap Dang Do ◽  
Hien Thi Dieu Huynh ◽  
The Danh Tran ◽  
Tuan Trong Tran
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Natural Light ◽  
Ex Vitro ◽  
Ambient Conditions ◽  
Potato Plants ◽  
Standard Culture ◽  
Survival And Growth ◽  
Better Than

Plantlets of sweet potato ( Ipomoea batatas L. ) were cultured in vitro under three different ambient conditions including a standard culture room - PS, a culture room inside a glasshouse with natural light but controlled temperature - TH, and a standard glasshouse with natural light (natural fluctuations of temperature) - NP. Plantlets from three treatments were compared in terms of pathogen rate, growth, survival plant at the end of the in vitro stage and at the ex vitro acclimatization. This result showed that, after 28 days of culture, sweet potato plants were cultured in vitro TH conditions have reduced entirely due to susceptibility to fungal disease causing outside air. After 14 days of ex vitro acclimatization, plants originally grow in vitro under the TH condition had ability to adapt about field survival and growth rates better than the other two treatments.

scholarly journals Enhancement of the Antiobesity and Antioxidant Effect of Purple Sweet Potato Extracts and Enhancement of the Effects by Fermentation

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 888
Author(s):  
Seul Gi Lee ◽  
Jongbeom Chae ◽  
Dong Se Kim ◽  
Jung-Bok Lee ◽  
Gi-Seok Kwon ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Radical Scavenging ◽  
The Body ◽  
Brown Adipocyte ◽  
Obese Mice ◽  
Adipose Tissues ◽  
White Adipocytes ◽  
Beige Adipocytes ◽  
Purple Sweet Potato

The browning of white adipocytes, which transforms energy-storing white adipocytes to heat-producing beige adipocytes, is considered a strategy against metabolic diseases. Several dietary compounds, such as anthocyanins, flavonoids, and phenolic acids, induce a brown adipocyte-like phenotype in white adipocytes. In this study, we demonstrated that purple sweet potato (Ipomoea batatas) extract (PSP) exhibited potent radical scavenging activity. In addition, PSP was found to contain large amounts of phenolic, flavonoid, and anthocyanin compounds; the amount of these compounds was affected by fermentation. Functionally, PSP-induced adipose browning in high-fat-diet (HFD)-induced obese mice. The administration of PSP significantly suppressed the body weight gain and abnormal expansion of white adipose tissues in the obese mice. The expression of adipose browning-related genes was higher in the inguinal white adipose tissues from the PSP-treated mice than those in the HFD-fed mice. Moreover, PSP-treated 3T3-L1 adipocytes formed multilocular lipid droplets, similar to those formed in the 3T3-L1 adipocytes treated with a browning induction cocktail. The PSP-treated cells had an increased expression level of mitochondria and lipolysis-related genes. The browning effects of PSP were enhanced by fermentation with Lactobacillus. This study, to our knowledge, is the first to identify a new mechanism to increase the antiobesity effects of PSP by inducing adipocyte browning of adipocytes.

scholarly journals Biofertlizer Lumbrical improves the growth and ex vitro acclimatization of micropropagated pear plants

2021 ◽  
Vol 22 (1) ◽  
pp. 17-30
Author(s):  
Nataliya Dimitrova ◽  
Lilyana Nacheva ◽  
Małgorzata Berova ◽  
Danuta Kulpa
Keyword(s):  
Woody Species ◽  
Photosynthetic Photon Flux Density ◽  
Stem Length ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Ex Vitro ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Number Of Leaves

In vitro micropropagation of plants is highly useful for obtaining large quantities of planting material with valuable economic qualities. However, plantlets grow in vitro in a specific environment and the adaptation after the transfer to ex vitro conditions is difficult. Therefore, the acclimatization is a key step, which mostly determines the success of micropropagation. The aim of this investigation was to study the effect of the biofertlizer Lumbrical on ex vitro acclimatization of micropropagated pear rootstock OHF 333 (Pyrus communis L.). Micropropagated and rooted plantlets were potted in peat and perlite (2:1) mixture with or without Lumbrical. They were grown in a growth chamber at a temperature of 22±2 °C and photoperiod of 16/8 hours supplied by cool-white fluorescent lamps (150 µmol m-2 s-1 Photosynthetic Photon Flux Density, PPFD). The plants were covered with transparent foil to maintain the high humidity, and ten days later, the humidity was gradually decreased. Biometric parameters, anatomic-morphological analyses, net photosynthetic rate and chlorophyll a fluorescence (JIP test) were measured 21 days after transplanting the plants to ex vitro conditions. The obtained results showed that the plants, acclimatized ex vitro in the substrate with Lumbrical, presented better growth (stem length, number of leaves, leaf area and fresh mass) and photosynthetic characteristics as compared to the control plants. This biostimulator could also be used to improve acclimatization in other woody species

scholarly journals Biochemistry of postharvest spoilage of sweet potato (Ipomoea batatas L.). 2. Comparison of cellulolytic enzyme production in cultures and fungi-infected sweet potato tubers

2006 ◽  
pp. 48-57
Author(s):  
R. C. Ray
Keyword(s):  
Sweet Potato ◽  
Enzyme Production ◽  
Ipomoea Batatas ◽  
Rhizopus Oryzae ◽  
Cellulose Synthesis ◽  
Cellulolytic Enzyme ◽  
Optimum Temperature ◽  
Potato Tubers

The study was conducted to determine the production in vitro and in vivo of cellulases by Botrydiplodia theobromae and Rhizopus oryzae. Isolates of these organisms were obtained from the postharvest decay of sweetpotato tubers. Results revealed that B. theobrornae and R. oryzae which were isolated from postharvest spoilage of sweetpotato tubers produced endo-13-1,4-glucanase and exo-V-1 ,4-glucanase in culture and in fungi-infected tissues of sweetpotato tubers. The optimum temperature and pH for cellulose synthesis and activity were 30°C and pH 6.5, respectively.

scholarly journals Effect of cytokinins and auxin on bud burst and direct organogenesis in vitro of some sweet potato landraces (Ipomoea batatas L.) grown in Benin

2019 ◽  
Vol 131 (1) ◽  
pp. 13347
Author(s):  
Arsène M. Doussoh ◽  
Justine Sossou Dangou ◽  
Gilles H.T. Cacaï ◽  
Serge S. Houedjissin ◽  
Corneille Ahanhanzo
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Direct Organogenesis ◽  
Bud Burst

scholarly journals Growth and Acclimation of In Vitro-Propagated M9 Apple Rootstock Plantlets under Various Visible Light Spectrums

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1017
Author(s):  
Guem-Jae Chung ◽  
Jin-Hui Lee ◽  
Myung-Min Oh
Keyword(s):  
Stomatal Conductance ◽  
Blue Light ◽  
Photosynthetic Rate ◽  
Light Quality ◽  
Red Light ◽  
High Ratio ◽  
The Other ◽  
Ex Vitro ◽  
Apple Rootstock

This study aimed to explore the suitable light quality condition for ex vitro acclimation of M9 apple plantlets. Light quality treatments were set as followed; monochromatic LEDs (red (R), green (G), blue (B)) and polychromatic LEDs (R:B = 7:3, 8:2 and 9:1; R:G:B = 6:1:3, 7:1:2 and 8:1:1). Plant height of R, R9B1, and R8G1B1 treatments were significantly higher than the other treatments. The number of leaves and SPAD value of B were significantly higher than the other treatments. Root fresh weights of R9B1 and R7G1B2 treatments showed an increase of at least 1.7-times compared to R, G and R8B2. R8G1B1 accumulated higher starch contents than the other treatments. Photosynthetic rate of R9B1 and R8B2 were significantly higher than the other treatments. In terms of stomatal conductance and transpiration rate, treatments with high blue ratio such as B, R7B3 had higher values. Rubisco concentration was high in R and B among monochromatic treatments. In conclusion, red light was effective to increase photosynthetic rate and biomass and blue light increased chlorophyll content and stomatal conductance. Therefore, for R9B1 and R8G1B1, a mixture of high ratio of red light with a little blue light would be proper for the acclimation of in vitro-propagated apple rootstock M9 plantlets to an ex vitro environment.

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