scholarly journals The impact of receptor recycling on the exocytosis of αvβ3 integrin targeted gold nanoparticles

Oncotarget ◽  
2017 ◽  
Vol 8 (24) ◽  
pp. 38618-38630 ◽  
Author(s):  
Yanan Cui ◽  
Xiaoning Song ◽  
Suxin Li ◽  
Bing He ◽  
Lan Yuan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Αvβ3 Integrin ◽  
Receptor Recycling ◽  
The Impact

Investigating the fate of gold nanoparticles taken up by trees through leaf and root pathways

2021 ◽  
Author(s):  
Paula Ballikaya ◽  
Ivano Brunner ◽  
Claudia Cocozza ◽  
Ralf Kaegi ◽  
Marcus Schaub ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Particulate Matter ◽  
Scots Pine ◽  
Tree Rings ◽  
Forest Ecosystems ◽  
Atmospheric Particulate Matter ◽  
Quantitative Risk Assessment ◽  
P Value ◽  
The Impact ◽  
Uptake And Transport

<p>Industrial activities and human population growth have resulted in an unprecedented increase in the release of particulate matter (PM) into the environment. Incidental nanoparticles (NPs) as a byproduct of industrial processes and engineered NPs are being discharged into terrestrial and aquatic ecosystems. Several studies on the impact of PM and NPs on human health have been conducted this century, but their effects on plants are poorly understood. What happens to them in forest ecosystems and trees has yet to be explored. The use of dendrochemistry to monitor air pollution is essential to provide past levels of contamination. Several studies have shown the ability of trees to accumulate pollutants into their annual rings, but the effect of particles at nano-scale is still largely unknown and their presence in tree rings unexplored.</p><p>In July 2019, a greenhouse experiment was conducted in order a) to confirm the uptake and transport of NPs in trees, b) to determine the delivery efficiency of different NPs entry pathways (leaves and roots), and c) to investigate the influence of surface-charged NPs on their uptake and transport. The fate of gold nanoparticles (AuNPs) was investigated in two tree species, European beech (<em>Fagus sylvatica</em> L.) and Scots pine (<em>Pinus sylvestris </em>L.). In the experiment, 40nm surface-charged AuNPs (positive, negative, and neutral AuNPs, hereafter referred to as treatments) were supplied once, separately to leaves and to roots. Twenty days after the treatment, Au concentration (mg kg<sup>-1</sup>) in leaves, stem and roots was determined by ICP-MS. In the leaf supply, Au concentrations were higher in leaves (98.3% and 99.2% on average, in beech and Scots pine respectively) and stems (1.4% and 0.45% on average) than in roots (0.3% and 0.35% on average). In the root supply, higher Au concentration was found in the roots (99.9 % on average in both species) than in the stems (0.1% on average in both species), whereas gold was not detected in the leaves. In the majority of cases, the measured Au was greater in beech than in Scots pine, probably due to their higher stomatal activity. AuNP concentrations among the treatments were significantly different (p value < 0.05), but distribution pattern in Scots pine were not discernible. In conclusion, AuNPs can be taken up by roots and leaves and transported to different compartments of trees. Different entry pathways influence the NP delivery within the plant tissues through transport mechanisms that are still unclear. It seems that NPs are allowed to move faster from the leaves through the phloem to the xylem and are further distributed throughout the plant system, including to the roots. The influence of surface-charged nanoparticles on their uptake and transport is not completely clear, and further research is needed in order to understand their behavior in trees.</p><p>This study shows the potential of trees as proxies to monitor NPs in forest ecosystems. Using tree rings as spatiotemporal indicators of the impact of particles on the environment will help a quantitative risk assessment and management of atmospheric particulate matter and NPs concentrations in the environment.</p>

scholarly journals Exposure to airborne gold nanoparticles: a review of current toxicological data on the respiratory tract

2020 ◽  
Vol 22 (8) ◽  
Author(s):  
Barbara De Berardis ◽  
Magda Marchetti ◽  
Anna Risuglia ◽  
Federica Ietto ◽  
Carla Fanizza ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Human Health ◽  
Large Scale ◽  
Current Knowledge ◽  
Engineered Nanoparticles ◽  
In Vivo Studies ◽  
Field Exposure ◽  
The Impact

AbstractIn recent years, the introduction of innovative low-cost and large-scale processes for the synthesis of engineered nanoparticles with at least one dimension less than 100 nm has led to countless useful and extensive applications. In this context, gold nanoparticles stimulated a growing interest, due to their peculiar characteristics such as ease of synthesis, chemical stability and optical properties. This stirred the development of numerous applications especially in the biomedical field. Exposure of manufacturers and consumers to industrial products containing nanoparticles poses a potential risk to human health and the environment. Despite this, the precise mechanisms of nanomaterial toxicity have not yet been fully elucidated. It is well known that the three main routes of exposure to nanomaterials are by inhalation, ingestion and through the skin, with inhalation being the most common route of exposure to NPs in the workplace. To provide a complete picture of the impact of inhaled gold nanoparticles on human health, in this article, we review the current knowledge about the physico-chemical characteristics of this nanomaterial, in the size range of 1–100 nm, and its toxicity for pulmonary structures both in vitro and in vivo. Studies comparing the toxic effect of NPs larger than 100 nm (up to 250 nm) are also discussed.

The impact of gold nanoparticles on hTERT gene expression leading to termination of malignant tumor

Gene ◽  
2012 ◽  
Vol 493 (1) ◽  
pp. 140-141 ◽  
Author(s):  
C. George Priya Doss ◽  
C. Debajyoti ◽  
S. Debottam
Keyword(s):  
Gene Expression ◽  
Gold Nanoparticles ◽  
Malignant Tumor ◽  
Htert Gene ◽  
The Impact

Effect of surface roughness on substrate-tuned gold nanoparticle gap plasmon resonances

Nanoscale ◽  
2015 ◽  
Vol 7 (9) ◽  
pp. 4250-4255 ◽  
Author(s):  
Chatdanai Lumdee ◽  
Binfeng Yun ◽  
Pieter G. Kik
Keyword(s):  
Surface Roughness ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Single Particle ◽  
Gold Films ◽  
Particle Scattering ◽  
Plasmon Resonances ◽  
Scattering Spectra ◽  
The Impact ◽  
Effect Of Surface

The impact of nanoscale surface roughness on substrate-tuned gold nanoparticle plasmon resonances is demonstrated by comparing single-particle scattering spectra with simulated scattering spectra of gold nanoparticles on gold films with realistic roughness.

scholarly journals Impact of Surface Modification with Gold Nanoparticles on the Bioelectrocatalytic Parameters of Immobilized Bilirubin Oxidase

Acta Naturae ◽  
2014 ◽  
Vol 6 (1) ◽  
pp. 102-106 ◽  
Author(s):  
D. V. Pankratov ◽  
Y. S. Zeifman ◽  
А. V. Dudareva ◽  
G. K. Pankratova ◽  
M. E. Khlupova ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Electron Transfer ◽  
Transfer Rate ◽  
Direct Electron Transfer ◽  
Electron Transfer Rate ◽  
Bilirubin Oxidase ◽  
Heterogeneous Electron Transfer ◽  
Enzyme Molecules ◽  
Different Diameters ◽  
The Impact

We unveil experimental evidence that put into question the widely held notion concerning the impact of nanoparticles on the bioelectrocatalytic parameters of enzymatic electrodes. Comparative studies of the bioelectrocatalytic properties of fungal bilirubin oxidase from Myrothecium verrucaria adsorbed on gold electrodes, modified with gold nanoparticles of different diameters, clearly indicate that neither the direct electron transfer rate (standard heterogeneous electron transfer rate constants were calculated to be 319 s -1) nor the biocatalytic activity of the adsorbed enzyme (bioelectrocatalytic constants were calculated to be 3411 s -1) depends on the size of the nanoparticles, which had diameters close to or larger than those of the enzyme molecules.

scholarly journals Gold nanoparticles-assisted plasmonic enhancement for DNA detection on the graphene-based portable SPR sensor

2020 ◽  
Author(s):  
Briliant Adhi Prabowo ◽  
Agnes Purwidyantri ◽  
Bei Liu ◽  
Hsin-Chih Lai ◽  
Kou-Chen Liu
Keyword(s):  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Limit Of Detection ◽  
Dna Detection ◽  
Integrated Approach ◽  
Optical Simulation ◽  
Range Limit ◽  
Plasmonic Enhancement ◽  
Spr Sensor ◽  
The Impact

The impact of different gold nanoparticles (GNPs) structures on the plasmonic enhancement for DNA detection is investigated on a few-layer graphene (FLG) surface plasmon resonance (SPR) sensor. Two distinct structures of gold nanourchins (GNu) and gold nanorods (GNr) were used to bind the uniquely designed single-stranded probe DNA (ssDNA) of Mycobacterium tuberculosis complex (MTBC) DNA. The two types of GNPs-ssDNA mixture were adsorbed onto the FLGcoated SPR sensor through the π-π stacking force between the ssDNA and the graphene layer. In the presence of the complementary single-stranded DNA (cssDNA), the hybridization process took place and gradually removed the probes from the graphene surface. From SPR sensor preparation, the annealing process of the Au layer of the SPR sensor effectively enhanced the FLG coverage leading to a higher load of the probe DNA onto the sensing interface. The FLG was shown effective in providing a larger surface area for biomolecular capture due to its roughness. Carried out in the DNA hybridization study with SPR sensor, GNu, with its rough and spikey structures, significantly reinforced the overall DNA hybridization signal than the GNr with smooth superficies, especially in capturing the probe DNA. The DNA hybridization detection assisted by GNu reached the femtomolar range limit of detection (LoD). An optical simulation validated the extreme plasmonic field enhancement at the tip of the GNu spicules. The overall integrated approach of graphene-based SPR sensor and GNu-assisted DNA detection provided the proof-of-concept for the possibility for Tuberculosis disease screening using a low-cost and portable system potentially applied in remote or third world countries.

scholarly journals Impact of Gold Nanoparticles on the Functions of Macrophages and Dendritic Cells

2020 ◽  
Author(s):  
Arindam K Dey ◽  
Alexis Gonon ◽  
Eve Isabelle Pécheur ◽  
Mylène Pezet ◽  
Christian Villiers ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dendritic Cells ◽  
Immune System ◽  
Metabolic Activity ◽  
Immune Cells ◽  
Cell Activation ◽  
Interleukin 4 ◽  
No Production ◽  
Phagocytic Capacity ◽  
The Impact

Abstract Background:Gold nanoparticles (AuNPs) have demonstrated outstanding performance in various biomedical applications, but their effects on the immune system remain ill-defined. We studied the impact of AuNPs on antigen-presenting cells (APCs) because of their phagocytic capacity that allows the accumulation of exogenous materials. As models, we used primary macrophages (M) and dendritic cells (DCs) originating from the bone marrow and tested the modulation of their functions, including phagocytosis, cell activation, production of cytokines and mediators and metabolic activity.Results: The AuNPs by themselves displayed no significant effect on M and DCs functions. However, when exposed to AuNPs, M and DCs responded differently to lipopolysaccharide (LPS) or Interleukin- 4(IL-4) stimulations. We showed AuNPs altered cytokine and reactive oxygen species (ROS) productions differently in M and DCs, whereas nitric oxide (NO) production by both cells remained unaffected. The metabolic profile underpins all functions of the immune cells and their polarisation. The analysis of the metabolic activity revealed that AuNPs significantly altered mitochondrial respiration and glycolysis of M, while only little effect was seen on DCs. Furthermore, we showed that T cell responses increased when antigen was presented by AuNPs-exposed DCs, leading to stronger Th1, Th2, and Th17 responses. Conclusions: Our data provide new insights into the complexity of the effects of AuNPs on the immune system. Although AuNPs may be considered on the whole to be devoid of direct significant effect, they may induce discrete modifications on some functions that can differ among the immune cells.

A carbon ink screen-printed immunoelectrode for Dengue virus NS1protein detection based on photosynthesized amine gold nanoparticles

2018 ◽  
pp. 1-12
Keyword(s):  
Gold Nanoparticles ◽  
Dengue Virus ◽  
Limit Of Detection ◽  
Colloidal Suspension ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Vis Spectroscopy ◽  
Label Free Detection ◽  
The Impact ◽  
Screen Printed

Dengue virus (DENV) is a reemerging mosquito-borne disease that is endemic in more than 125 countries, affecting 200 million people per year. Screening testing has been a good attempt to minimize the impact caused by high morbity and mortality rates of DENV. In this study, a simple and disposable label-free electrochemical immunosensor based on a carbon ink graphite screen-printed electrode (SPE) one-step fabricated was developed for detection of non-structural 1 protein (NS1). The SPE surface was modified by drop casting, depositing a colloidal suspension containing amine-functionalized gold nanoparticles (AuNP-NH2). AuNPs were synthetized by a photoinduced physical method, illuminating preformed gold seeds with a light-emitting diode (LED,) at blue region, by using the polyethyleneimine (NH2) as reductor and stabilizing agent. UV-VIS spectroscopy and Transmission Electron Microscopy (TEM) were used to characterize the amine AuNPs. Electrocatalytic activity of AuNPs allowed more sensitivity for a label-free detection of NS1 by square wave voltammetry (SWV), with linear response from 0.1 to 2 µg mL-1. It was found a good linearity (coefficient of correlation of 0.995 (p<0.01) and a limit of detection of 0.03 µg mL-1 NS1 for analytical responses. AuNP-NH2 synthesis provided an easy oriented immobilization of anti-NS1 antibodies by Fc portion, resulting in a simple fabrication immunosensor with relative high performance and feasibility for early diagnostic of DENV.

Adsorption of ozone and plasmonic properties of gold hydrosol: the effect of the nanoparticle size

2015 ◽  
Vol 17 (28) ◽  
pp. 18431-18436 ◽  
Author(s):  
Boris G. Ershov ◽  
Evgeny V. Abkhalimov ◽  
Vyacheslav I. Roldughin ◽  
Viktor M. Rudoy ◽  
Olga V. Dement'eva ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Plasmon Resonance ◽  
Bathochromic Shift ◽  
Resonance Peak ◽  
Nanoparticle Size ◽  
Plasmon Resonance Peak ◽  
The Impact

The impact of the size of gold nanoparticles on the magnitude of the bathochromic shift of their plasmon resonance peak upon ozone adsorption is revealed and analyzed.

Sign in / Sign up

Export Citation Format

Share Document