The component formula of Salvia miltiorrhiza and Panax ginseng induces apoptosis and inhibits cell invasion and migration through targeting PTEN in lung cancer cells

BACKGROUND: The incidence of lung cancer in Yunnan area ranks firstly in the world and underlying molecular mechanisms of lung cancer in Yunnan region are still unclear. We screened a novel potential oncogene CYP2S1 used mRNA microassay and bioinformation database. The function of CYP2S1 in lung cancer has not been reported. OBJECTIVE: To investigate the functions of CYP2S1 in lung cancer. METHODS: Immunohistochemistry and Real-time PCR were used to verify the expression of CYP2S1. Colony formation and Transwell assays were used to determine cell proliferation, invasion and migration. Xenograft assays were used to detected cell growth in vivo. RESULTS: CYP2S1 is significantly up-regulated in lung cancer tissues and cells. Knockdown CYP2S1 in lung cancer cells resulted in decrease cell proliferation, invasion and migration in vitro. Animal experiments showed downregulation of CYP2S1 inhibited lung cancer cell growth in vivo. GSEA analysis suggested that CYP2S1 played functions by regulating E2F targets and G2M checkpoint pathway which involved in cell cycle. Kaplan-Meier analysis indicated that patients with high CYP2S1 had markedly shorter event overall survival (OS) time. CONCLUSIONS: Our data demonstrate that CYP2S1 exerts tumor suppressor function in lung cancer. The high expression of CYP2S1 is an unfavorable prognostic marker for patient survival.

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Hsa-miR-125a-3p and hsa-miR-125a-5p are downregulated in non-small cell lung cancer and have inverse effects on invasion and migration of lung cancer cells

BMC Cancer ◽

10.1186/1471-2407-10-318 ◽

2010 ◽

Vol 10 (1) ◽

Cited By ~ 141

Author(s):

Lili Jiang ◽

Qin Huang ◽

Siyang Zhang ◽

Qingfu Zhang ◽

Jihong Chang ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cancer Cells ◽

Cell Lung Cancer ◽

Small Cell ◽

Lung Cancer Cells ◽

Small Cell Lung ◽

Invasion And Migration ◽

And Migration

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LncRNA DLX6-AS1 promotes the proliferation, invasion, and migration of non-small cell lung cancer cells by targeting the miR-27b-3p/GSPT1 axis

OncoTargets and Therapy ◽

10.2147/ott.s196865 ◽

2019 ◽

Vol Volume 12 ◽

pp. 3945-3954 ◽

Cited By ~ 12

Author(s):

Wen Sun ◽

Liwen Zhang ◽

Ranran Yan ◽

Ying Yang ◽

Xiangli Meng

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cancer Cells ◽

Cell Lung Cancer ◽

Small Cell ◽

Lung Cancer Cells ◽

Small Cell Lung ◽

Invasion And Migration ◽

And Migration

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Ectopic Production of MDA-7/IL-24 Inhibits Invasion and Migration of Human Lung Cancer Cells

Molecular Therapy ◽

10.1016/j.ymthe.2004.01.019 ◽

2004 ◽

Vol 9 (4) ◽

pp. 510-518 ◽

Cited By ~ 69

Author(s):

Rajagopal Ramesh ◽

Isao Ito ◽

Began Gopalan ◽

Yuji Saito ◽

Abner M Mhashilkar ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Human Lung ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Invasion And Migration ◽

Human Lung Cancer Cells ◽

Ectopic Production ◽

And Migration

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lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 (GLI2)

Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics ◽

10.3727/096504018x15220594629967 ◽

2019 ◽

Vol 27 (3) ◽

pp. 359-369 ◽

Cited By ~ 10

Author(s):

Hongliang Yang ◽

Lei Yan ◽

Kai Sun ◽

Xiaodong Sun ◽

Xudong Zhang ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cancer Cells ◽

Cell Lung Cancer ◽

Small Cell ◽

Lung Cancer Cells ◽

Small Cell Lung ◽

Invasion And Migration ◽

And Migration

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Pumilio RNA Binding Family Member 2 Promotes the Proliferation and Metastasis of Lung Cancer Cells by Regulating Ca2+ Signaling Pathway via Targeting C-X-C Chemokine Receptor Type 4

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2692 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1339-1345

Author(s):

Zhijie Shang ◽

Yuxuan Wang ◽

Lixun Chai ◽

Gengpu Yang

Keyword(s):

Lung Cancer ◽

Western Blot ◽

Cancer Cells ◽

Signaling Pathway ◽

Chemokine Receptor ◽

Rna Binding ◽

Lung Cancer Cells ◽

Invasion And Migration ◽

Proliferation And Metastasis ◽

And Migration

The aim of the present study was to investigate the mechanism by which pumilio RNA binding family member 2 (PUM2), an RNA-binding protein (RBP) of C-X-C chemokine receptor type 4 (CXCR4), exerts its effects on the development of lung cancer. RT-qPCR and western blot analysis were utilized to measure the expression of PUM2 in several lung cancer cell lines. Cell Counting Kit-8 (CCK-8), colony formation assay, transwell- and wound healing assays were employed to determine the proliferation, invasion and migration of NCI-H520 cells, respectively. Next, the expression of CXCR4 was measured using western blot analysis, and the combination between PUM2 and CXCR4 was verified by RNA immunoprecipitation (RIP) assay and RNA pull down assay. Finally, whether the expression of PUM2 can affect the Ca2+ signaling pathway was confirmed by western blot assay. Results revealed that the expression level of PUM2 was notably upregulated in lung cancer cells, and knockdown of PUM2 significantly inhibited the proliferation, invasion and migration of NCI-H520 cells. PUM2 was confirmed to be the RBP of CXCR4, and PUM2 knockdown decreased the expression of CXCR4. In addition, PUM2 silencing inhibited the phosphorylation of CaMKII, ERK, and MEK. Taken together, these findings demonstrated that PUM2 could promote the proliferation and metastasis of lung cancer cells by regulating Ca2+ signaling pathway via targeting CXCR4, which may provide a novel insight for the future treatment of lung cancer.

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