scholarly journals Characteristics and genetic diversity of multi-drug resistant extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolated from bovine mastitis

Oncotarget ◽  
2017 ◽  
Vol 8 (52) ◽  
pp. 90144-90163 ◽  
Author(s):  
Tariq Ali ◽  
Sadeeq ur Rahman ◽  
Limei Zhang ◽  
Muhammad Shahid ◽  
Dandan Han ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Bovine Mastitis ◽  
Beta Lactamase ◽  
Drug Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

scholarly journals Genetic characterization of AmpC and extended-spectrum beta-lactamase (ESBL) phenotypes in Escherichia coli and Salmonella from Alberta poultry

2020 ◽  
Author(s):  
Tam Tran ◽  
Sylvia Checkley ◽  
Niamh Caffrey ◽  
Rashed Cassis ◽  
Chunu Mainali ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Beta Lactamase ◽  
Drug Resistant ◽  
Gene Encoding ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Class 1

AbstractHorizontal gene transfer is an important mechanism which facilitates bacterial populations in overcoming antimicrobial treatment. In this study, a total of 120 Escherichia coli and 62 Salmonella enterica subsp. enterica isolates were isolated from poultry farms in Alberta. Fourteen serovars were identified among Salmonella isolates. Thirty one percent of E. coli isolates were multiclass drug resistant (resistant to ≥ 3 drug classes), while only about 16% of Salmonella isolates were multiclass drug resistant. Among those, eight E. coli isolates had an AmpC-type phenotype, and one Salmonella isolate had an extended-spectrum beta-lactamase (ESBL)-type β-lactamase phenotype. We identified both AmpC-type (blaCMY-2) and ESBL-type (blaTEM) genes in both E. coli and Salmonella isolates. Plasmids from eight of nine E. coli and Salmonella isolates were transferred to recipient strain E. coli J53 through conjugation. Transferable plasmids in above total eight E. coli and Salmonella isolates were also transferred into a lab-made sodium azide-resistant Salmonella recipient through conjugation. The class 1 integrase gene, int1, was detected on plasmids from two E. coli isolates. Further investigation of class 1 integron cassette regions revealed the presence of an aadA gene encoding streptomycin 3”-adenylyltransferase, an aadA1a/aadA2 gene encoding aminoglycoside 3”-O-adenyltransferase, and a putative adenylyltransferase gene. This study provides some insight into potential horizontal gene transfer events of antimicrobial resistance genes between E. coli and Salmonella in poultry production.

scholarly journals Chorioamnionitis and Neonatal Sepsis Due to Extended Spectrum Beta-Lactamase producing Escherichia Coli Infection: A Case Report

2021 ◽  
Author(s):  
Saheed Shittu ◽  
Sufia Athar ◽  
Adila Shaukat ◽  
Lolwa Alansari
Keyword(s):  
Escherichia Coli ◽  
Neonatal Sepsis ◽  
Adverse Outcomes ◽  
Beta Lactamase ◽  
Drug Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Escherichia Coli Infection ◽  
Prolonged Hospital

Chorioamnionitis, a common complication of pregnancy is associated with significant maternal, perinatal, and long-term adverse outcomes. This case of placental infection leading to preterm delivery, severe neonatal sepsis, maternal wound infection, postnatal readmission, and prolonged hospital stay was caused by multi-drug resistant Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia Coli

scholarly journals Multi-drug Resistant (MDR), Extended Spectrum Beta-lactamase (ESBL) and New Delhi Metallo Beta-lactamase (NDM) Producing Escherichia Coli in Rescued Sloth Bears (Melursus Ursinus), India

2021 ◽  
Author(s):  
O.R. Vinodhkumar ◽  
M. Karikalan ◽  
S. Ilayaraja ◽  
Arun A Sha ◽  
B.R. Singh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Beta Lactamase ◽  
Drug Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Carbapenemase Gene ◽  
Sloth Bear ◽  
Melursus Ursinus

Abstract The study reports the MDR, ESBL, and NDM producing Escherichia coli (CRE) isolated from the rescued sloth bear (Melursus ursinus), India. The faecal samples of adult rescued sloth bear (n=21) were collected from a rescue center located in India during 2015-2016 and processed for isolation and antibacterial susceptibility pattern of E. coli. 45E. coli isolates were recovered, and on phenotypic screening, 23 were MDR, 17 were ESBL producers, and five were carbapenem-resistant (CR). The MDR isolates carried beta-lactamase, chloramphenicol, aminoglycosides, tetracycline, fluroquinone, and sulphadimidine resistance genes. All the phenotypic ESBL producing isolates had blaCTX-M genes. On genotypic screening, three CRE (60.0 %, 3/5) were positive for blaNDM carbapenemase gene. Efflux pump-mediated carbapenem resistance was noticed in two CRE isolates (40.0 %, 2/5).The CRE also isolates co-harbored AMR genes like blaTEM-1, blaAmpC, qnrA, qnrB, qnrS, tetA, tetB and sulI. Virulence screening of the resistant isolates revealed the presence of Stx1, Stx2, eae, hlyA genes.Plasmid incompatibility (Inc) typesof three NDM positive isolates revealed that two isolates blaNDM-5 gene on Incl1 an one isolate on IncF plasmid. Apart from NDM genes, the plasmids also carried tetracycline, beta-lactamase and quinolone resistance genes. The plasmid multilocus sequence typing (pMLST) of the E. coli Incl1 plasmid showed the Sequence Type (ST) 297.This appears to be the first report of multi-drug resistant, extended spectrum beta-lactamase (ESBL) producing and blaNDM-5geneson Incl1 and IncF plasmids in rescued sloth bear.

scholarly journals Multidrug-Resistant, Including Extended-Spectrum Beta Lactamase-Producing and Quinolone-Resistant, Escherichia coli Isolated from Poultry and Domestic Pigs in Dar es Salaam, Tanzania

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 406
Author(s):  
Zuhura I. Kimera ◽  
Fauster X. Mgaya ◽  
Gerald Misinzo ◽  
Stephen E. Mshana ◽  
Nyambura Moremi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Antibiotics Resistance ◽  
Beta Lactamase ◽  
Domestic Pigs ◽  
Extended Spectrum Beta Lactamase ◽  
Phenotypic Profile ◽  
E Coli ◽  
Extended Spectrum ◽  
Esbl Producers

We determined the phenotypic profile of multidrug-resistant (MDR) Escherichia coli isolated from 698 samples (390 and 308 from poultry and domestic pigs, respectively). In total, 562 Enterobacteria were isolated. About 80.5% of the isolates were E. coli. Occurrence of E. coli was significantly higher among domestic pigs (73.1%) than in poultry (60.5%) (p = 0.000). In both poultry and domestic pigs, E. coli isolates were highly resistant to tetracycline (63.5%), nalidixic acid (53.7%), ampicillin (52.3%), and trimethoprim/sulfamethoxazole (50.9%). About 51.6%, 65.3%, and 53.7% of E. coli were MDR, extended-spectrum beta lactamase-producing enterobacteriaceae (ESBL-PE), and quinolone-resistant, respectively. A total of 68% of the extended-spectrum beta lactamase (ESBL) producers were also resistant to quinolones. For all tested antibiotics, resistance was significantly higher in ESBL-producing and quinolone-resistant isolates than the non-ESBL producers and non-quinolone-resistant E. coli. Eight isolates were resistant to eight classes of antimicrobials. We compared phenotypic with genotypic results of 20 MDR E. coli isolates, ESBL producers, and quinolone-resistant strains and found 80% harbored blaCTX-M, 15% aac(6)-lb-cr, 10% qnrB, and 5% qepA. None harbored TEM, SHV, qnrA, qnrS, qnrC, or qnrD. The observed pattern and level of resistance render this portfolio of antibiotics ineffective for their intended use.

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