Effect of supplementing Aegle marmelos leaves on in vitro rumen fermentation and methanogenesis of diets varying in roughage to concentrate ratio

The present study was undertaken to observe the effect of supplementing Aegle marmelos leaves on in vitro rumen fermentation and methane production of different diets containing varied proportion of roughage (R) to concentrate (C). Aegle marmelos (Bael) leaves was supplemented at 4 levels (0, 5, 10 and 15%) to three diets having roughage (R) and concentrate (C) (wheat straw and concentrate mixture) in the ratio 70:30, 50:50 and 30:70. Inclusion of Aegle leaves at different levels showed significant rise in total gas (mL/g DM) among different diets. Similarly, Aegle marmelos leaves at the rate of 15% improved (P>0.05) the IVTDMD and IVTOMD (%) of the experimental diets. Among the VFA’s acetate concentration (mM) was significantly enhanced at 15% of Aegle marmelos supplementation. No significant effect was observed in the values of CH4 (%, mL/24h and mL/100mg DDM) and NH3-N (mg/dL) on supplementation of Aegle leaves if any of the diets. Therefore, it can be concluded from the present findings that supplementation of Aegle marmelos leaves enhances the digestibility hence it can be used to improve the nutritive value of diets.

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Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

SN Applied Sciences ◽

10.1007/s42452-021-04264-6 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Avijit Dey ◽

Shyam Sundar Paul ◽

Puran Chand Lailer ◽

Satbir Singh Dahiya

Keyword(s):

Environmental Pollution ◽

Methane Production ◽

Rumen Fluid ◽

Bubalus Bubalis ◽

Rumen Fermentation ◽

Acetyl Esterase ◽

Garlic Oil ◽

Enteric Methane ◽

In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

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