scholarly journals In vitro rumen fermentation and methane production as affected by rambutan peel powder

2017 ◽  
Vol 46 (1) ◽  
pp. 626-631 ◽  
Author(s):  
Pongsatorn Gunun ◽  
Nirawan Gunun ◽  
Anusorn Cherdthong ◽  
Metha Wanapat ◽  
Sineenart Polyorach ◽  
...  
2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.


2022 ◽  
Vol 10 (1) ◽  
pp. 1-9
Author(s):  
Aarón A. Molho-Ortiz ◽  
Atmir Romero-Pérez ◽  
Efrén Ramírez-Bribiesca ◽  
Claudia C. Márquez-Mota ◽  
Francisco A. Castrejón-Pineda ◽  
...  

2020 ◽  
Vol 52 (5) ◽  
pp. 2433-2441
Author(s):  
Lais Micaelle Lopes Moura ◽  
Flávia Denise da Silva Pereira ◽  
Patrícia Rodrigues de Lima ◽  
Júlio Cesar Silva Nascimento ◽  
Andresca dos Santos de Oliveira ◽  
...  

2021 ◽  
Vol 51 (2) ◽  
pp. 212-220
Author(s):  
L. A. Mejía Uribe ◽  
I. A. Domínguez Vara ◽  
F. Hernández Ruipérez ◽  
A. A. Rayas Amor ◽  
E. Morales Almaráz

The objective was to investigate the effect of replacing maize silage (MS) with canola silage (CS) on the chemical composition and fatty acid (FA) profile of total mixed rations (TMR) containing these silages, and on in vitro rumen fermentation and methane production from them. The canola (Brassica napus var. Monty) was cultivated on a small-scale agricultural farm and harvested at 148 days after sowing. Maize silage in a TMR was replaced with 0%, 15%, 25%, and 35% CS to make the rations CS0, CS15, CS25, and CS35, respectively. Proximate analyses of the rations were evaluated in a completely randomized design. The results showed that linolenic acid increased linearly with the level of CS, primarily at the expense of linoleic acid. In vitro dry matter digestibility (IVDMD) was similar among treatments. However, in vitro neutral detergent fibre digestibility (IVNDF) decreased linearly (P <0.05) when the CS proportion increased in the TMR. The lowest ammonia nitrogen content (P <0.05) was observed in CS35. The soluble fraction (A) increased (P <0.05) when the CS increased in the TMR from 0% to 35%. In vitro methane (CH4) production was lowest with CS25 and CS35, decreasing 34% and 23.9%, respectively, compared with CS0. Linolenic acid had a negative correlation with IVNDF (r = -0.94; P <0.05). The IVDMD and methane production were positively correlated (r = 0.60) (P <0.05). In conclusion, 25% and 35% augmentation of MS with CS in a TMR was an important source of linolenic acid (C18:3) and decreased in vitro methane production.


2013 ◽  
Vol 64 (9) ◽  
pp. 935 ◽  
Author(s):  
B. K. Banik ◽  
Z. Durmic ◽  
W. Erskine ◽  
K. Ghamkhar ◽  
C. Revell

Thirteen current and potential pasture species in southern Australia were examined for differences in their nutritive values and in vitro rumen fermentation profiles, including methane production by rumen microbes, to assist in selection of pasture species for mitigation of methane emission from ruminant livestock. Plants were grown in a glasshouse and harvested at 7 and 11 weeks after sowing for in vitro batch fermentation, with nutritive values assessed at 11 weeks of growth. The pasture species tested differed significantly (P < 0.001) in methane production during in vitro rumen fermentation, with the lowest methane-producing species, Biserrula pelecinus L., producing 90% less methane (4 mL CH4 g–1 dry matter incubated) than the highest methane-producing species, Trifolium spumosum L. (51 mL CH4 g–1 dry matter incubated). Proxy nutritive values of species were found not to be useful predictors of plant fermentation characteristics or methane production. In conclusion, there were significant differences in fermentative traits, including methane production, among selected pasture species in Australia, indicating that the choice of fodder species may offer a way to reduce the impact on the environment from enteric fermentation.


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