scholarly journals Evaluating the Efficiency of Newly Formulated Pomade® and Ceftiofur Hydrochloride for Treating Foot Rot in Dairy Cattle

2021 ◽  
Author(s):  
K. Yiğitarslan ◽  
M. Kale ◽  
D. Öztürk ◽  
N. Mamak
Keyword(s):  
Dairy Cattle ◽  
Economic Loss ◽  
Treatment Method ◽  
Fusobacterium Necrophorum ◽  
Foot Rot ◽  
Chain Reaction ◽  
Dichelobacter Nodosus ◽  
Foot Disease ◽  
New Treatment ◽  
Ceftiofur Hydrochloride

Background: Foot rot is an important contagious disease that causes economic loss in dairy cattle. Even though many antibiotic treatments have been tried on foot rot, very few information about new topical treatment method or product have been reported about the disease. The main objective of this paper is to evaluate an alternative new treatment for foot root disease in dairy cattle. Methods: Forty-one swap samples were collected from cattle’s feet which were further investigated under microscopic examination and polymerase chain reaction (PCR) for Dichelobacter nodosus and Fusobacterium necrophorum. Newly formulated pomade® and ceftiofur hydrochloride (Eficur®) applications were used in foot rot cases. Result: Polymerized chain reaction revealed D. nodosus in all the samples were as F. necrophorum was seen only in 22 (53.66%) samples. In this study, D. nodosus was considered as the primary agent involved in foot disease of cattle and F. necrophorum along with other bacterias were considered to be associated in the infection. The foot rot wounds formed in all cases (100%) in which D. nodosus, F. necrophorum and other bacteria were detected were healed along with tissue regeneration. As a result of treatment applications, a success rate of 93.33% was obtained in lameness resulting due to medium foot rot cases and 45.45% in severe acute lameness. The lameness recovery rate was found to be 80.48%.

scholarly journals Pooling of interdigital swab samples for PCR detection of virulent Dichelobacter nodosus

2017 ◽  
Vol 30 (2) ◽  
pp. 205-210 ◽  
Author(s):  
Deborah Greber ◽  
Iwan Locher ◽  
Peter Kuhnert ◽  
Marc-André Butty ◽  
Kerstin Holdener ◽  
...  
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
Real Time Pcr ◽  
Substantial Reduction ◽  
Control Program ◽  
Foot Rot ◽  
Dichelobacter Nodosus ◽  
Foot Disease ◽  
Development And Validation ◽  
Adequate Method

Virulent ovine foot rot is a contagious foot disease. Given the development and validation of a real-time PCR to detect Dichelobacter nodosus isolates that contain the virulence-associated protease genes aprV2 and aprB2, the diagnosis of foot rot has made considerable progress. We evaluated pooling methods to reduce the number of samples during a foot rot control program. Samples of individual feet were compared to a 4-feet sample of the same sheep. All further analyses based on 4-feet samples (pools-of-5 and pools-of-10 4-feet samples) were compared to samples of individual sheep, and a risk-based herd sampling was evaluated and compared to the whole flock. The sensitivity and specificity of the 4-feet samples for detection of aprV2-positive strains was 93.8% (CI: 87.6–97.5%) and 98.3% (CI: 96.5–99.3%), respectively. The sensitivity and specificity of the pools-of-10 was 86.7% (CI: 78.4–92.7%) and 100.0% (CI: 97.4–100%), respectively. Pools-of-5 were not significantly more sensitive than pools-of-10. The pooling of 4 individual foot samples into one 4-feet sample is an adequate method to reduce the number of samples of individual sheep. The sensitivity of pools-of-5 and pools-of-10 is too imprecise for a control program. Risk-based sampling allowed for a substantial reduction of samples to be tested, had a sensitivity of 95.8% (CI: 78.9–99.9%) and specificity of 100.0% (CI: 88.1–100.0%) when determining the foot rot flock status, and represents an adequate methodology to predict within-flock freedom from infection.

scholarly journals Contagious Ovine Digital Dermatitis: A Novel Bacterial Etiology and Lesion Pathogenesis

2021 ◽  
Vol 8 ◽  
Author(s):  
Gareth J. Staton ◽  
Joseph W. Angell ◽  
Dai Grove-White ◽  
Simon R. Clegg ◽  
Stuart D. Carter ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bacterial Colonization ◽  
Fusobacterium Necrophorum ◽  
Digital Dermatitis ◽  
Dichelobacter Nodosus ◽  
Copy Numbers ◽  
Colonization Patterns ◽  
Foot Disease ◽  
Long Acting ◽  
The Uk

Contagious ovine digital dermatitis (CODD) is a severe and common infectious foot disease of sheep and a significant animal welfare issue for the sheep industry in the UK and some European countries. The etiology and pathogenesis of the disease are incompletely understood. In this longitudinal, experimental study, CODD was induced in 18 sheep, and for the first time, the clinical lesion development and associated microbiological changes in CODD affected feet are described over time, resulting in a completely new understanding of the etiopathogenesis of CODD. The majority of CODD lesions (83.9%) arose from pre-existing interdigital dermatitis (ID) and/or footrot (FR) lesions. All stages of foot disease were associated with high levels of poly-bacterial colonization with five pathogens, which were detected by quantitative PCR (qPCR): Treponema medium, Treponema phagedenis, Treponema pedis, Dichelobacter nodosus, and Fusobacterium necrophorum. Temporal colonization patterns showed a trend for early colonization by T. phagedenis, followed by F. necrophorum and D. nodosus, T. medium, and then T. pedis, D. nodosus was present at significantly higher predicted mean log10 genome copy numbers in FR lesions compared to both ID and CODD, while Treponema species were significantly higher in CODD and FR lesions compared to ID lesions (p < 0.001). Treatment of CODD-affected sheep with two doses of 10 mg/kg long acting amoxicillin resulted in a 91.7% clinical cure rate by 3 weeks post-treatment; however, a bacteriological cure was not established for all CODD-affected feet. The study found that in an infected flock, healthy feet, healed CODD feet, and treated CODD feet can be colonized by some or all of the five pathogens associated with CODD and therefore could be a source of continued infection in flocks. The study is an experimental study, and the findings require validation in field CODD cases. However, it does provide a new understanding of the etiopathogenesis of CODD and further supportive evidence for the importance of current advice on the control of CODD; namely, ensuring optimum flock control of footrot and prompt isolation and effective treatment of clinical cases.

scholarly journals Foot rot and other foot diseases of goat and sheep in the semiarid region of northeastern Brazil

2011 ◽  
Vol 31 (10) ◽  
pp. 879-884 ◽  
Author(s):  
Gildeni M.N Aguiar ◽  
Sara V.D Simões ◽  
Tatiane R Silva ◽  
Adriana C.O Assis ◽  
João M.A Medeiros ◽  
...  
Keyword(s):  
Fusobacterium Necrophorum ◽  
Control Measures ◽  
Northeastern Brazil ◽  
Semiarid Region ◽  
Common Disease ◽  
Foot Rot ◽  
White Line ◽  
Dichelobacter Nodosus ◽  
Foot Diseases ◽  
Sole Ulcers

This paper reports the occurrence and epidemiology of outbreaks of foot rot and other foot diseases in goats and sheep in the semiarid region of Paraíba, northeastern Brazil. Four farms were inspected for the presence of foot lesion in sheep and goats and for environmental conditions, general hygiene, pastures, and disease control measures. The prevalence of foot lesions was 19.41% (170/876) in sheep and 17.99% (52/289) in goats, ranging between 5.77% and 33.85% in different farms. Foot rot was the most common disease, affecting 12.1% of the animals examined (141/1165), but with significantly higher (p<0.05) prevalence in sheep (13.69%) than in goats (7.27%). The frequency of malignant foot rot was also significantly lower (p<0.05) in goats (9.53%) than in the sheep (40.83%). On one farm, Dorper sheep showed significantly higher (p<0.05) prevalence of foot rot (17.5%) than Santa Inês sheep (6.79%), and the number of digits affected was also higher in the former. Dichelobacter nodosus and Fusobacterium necrophorum were isolated from cases of foot rot. White line disease was found in 3.95% of the animals, sole ulcers in 1.29%, foot abscess in 1.03% and hoof overgrowth in 0.5%. The high rainfall at the time of occurrence, grazing in wetlands, clay soils with poor drainage, presence of numerous stony grounds, closure of the flocks in pens at night, and introduction of affected animals were considered predisposing factors for the occurrence of foot diseases.

scholarly journals Isolation and identification of Dichelobacter nodosus and Fusobacterium necrophorum using the polymerase chain reaction method in sheep with footrot

2015 ◽  
Vol 84 (2) ◽  
pp. 97-104 ◽  
Author(s):  
Ediz Kagan Ozgen ◽  
Seyda Cengiz ◽  
Mustafa Ulucan ◽  
Zafer Okumus ◽  
Asli Kortel ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Small Ruminants ◽  
Fusobacterium Necrophorum ◽  
Chain Reaction ◽  
Culture Methods ◽  
Isolation And Identification ◽  
Dichelobacter Nodosus ◽  
Specificity And Sensitivity ◽  
Pcr Method ◽  
Polymerase Chain

Footrot is an important infectious disease of small ruminants leading to severe economical losses. The aim of the present study was to determine isolation and identification rates ofDichelobacter nodosusandFusobacterium necrophorumin the culture techniques and reveal the specificity and sensitivity of the culture technique based on the polymerase chain reaction (PCR) method in sheep with footrot. Dry swabs and swabs with Amies medium from 83 sheep were subjected to PCR and culture analyses. In dry swabs, 4 samples were positive forF. necrophorumand all were negative forD. nodosus. Colonies in Eugon and Fusobacterium selective agars from swabs with Amies medium were evaluated. Polymerase chain reaction analysis was conducted on macroscopically and microscopically unidentified samples. The positivity rate was 55.4% forD. nodosusand 69.8% forF. necrophorumin cultures from Fusobacterium selective agars. The positivity rate forD. nodosusin Fusobacterium selective agars was higher than that in Eugon agar. Performing PCR and culture methods increased positivity as compared to performing them alone. In comparison with the PCR method, culturing in Fusobacterium selective agars had moderate sensitivity and low specificity forD. nodosus(71.7 and 28.7%) andF. necrophorum(61.3 and 80.0%), respectively. In conclusion, Fusobacterium selective agar (without antibiotics) for isolation and identification ofD. nodosusis superior to Eugon agar.Fusobacterium necrophorumshould also be considered as a provoking agent for footrot in small ruminants. The PCR method on culture increases elucidation of definitive aetiology.

scholarly journals Detection of Fusobacterium necrophorum and Dichelobacter nodosus from cow footrot in the Heilongjiang Province, China

2019 ◽  
Vol 88 (2) ◽  
pp. 165-168
Author(s):  
Jiaqi Han ◽  
Jing Dong ◽  
Wenhui Yang ◽  
Yunze Xu ◽  
Qiming Xing ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Northeast China ◽  
Pcr Primers ◽  
Fusobacterium Necrophorum ◽  
Heilongjiang Province ◽  
Chain Reaction ◽  
Dichelobacter Nodosus ◽  
Important Concern ◽  
Frequent Finding ◽  
Polymerase Chain

Cow footrot in the Heilongjiang Province, northeast China is a problem resulting in lost production in agriculture. In this study, 200 swab samples from footrot lesions of naturally infected cows with odorous exudative inflammation and keratinous hoof separation at 10 farms were examined in the period from May 2016 to May 2017. Twenty cows from each farm were taken for sampling. The samples were examined for detectingthe presence of Dichelobacter nodosus (D. nodosus) and Fusobacterium necrophorum (F. necrophorum). Such detection was carried out using polymerase chain reaction (PCR). The PCR primers were designed to identify the lktA gene, which encodes a leukotoxin unique to F. necrophorum, and the fimA gene of D. nodosus. Of the 200 samples, 111 (55.5%) revealed the presence of F. necrophorum and 11 (5.5%) exhibited D. nodosus. The frequent finding of F. necrophorum in cow farms of Heilongjiang province, northeast China is noteworthy. The possibility of F. necrophorum and D. nodosus infection should be an important concern when controlling cow footrot in China.

Comparison of milk production, mastitis, lameness and days to conception of Brown Swiss, Holstein and Holstein cross Brown Swiss dairy cattle

2006 ◽  
Vol 2006 ◽  
pp. 86-86
Author(s):  
G Wellwood ◽  
J K Margerison
Keyword(s):  
Dairy Cattle ◽  
Somatic Cell ◽  
Milk Production ◽  
Milk Yield ◽  
Complex Disease ◽  
Economic Loss ◽  
Brown Swiss ◽  
Somatic Cell Counts ◽  
Cell Counts ◽  
Holstein Friesian

Mastitis is a complex disease causing inflammation of the udder, which has been estimated to cost the dairy farmer between £40-£117/cow per year (Stott et al., 2002). Economic loss occurs as a result of discarded milk, reduced milk yield and milk quality, increased vet costs and an increase in replacement costs. The objective of this study was to examine the effect of breed on the incidence of mastitis and somatic cell counts and milk production capabilities of Holstein Friesian, Brown Swiss and Brown Swiss crossbred cows.

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