scholarly journals Contagious Ovine Digital Dermatitis: A Novel Bacterial Etiology and Lesion Pathogenesis

2021 ◽  
Vol 8 ◽  
Author(s):  
Gareth J. Staton ◽  
Joseph W. Angell ◽  
Dai Grove-White ◽  
Simon R. Clegg ◽  
Stuart D. Carter ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bacterial Colonization ◽  
Fusobacterium Necrophorum ◽  
Digital Dermatitis ◽  
Dichelobacter Nodosus ◽  
Copy Numbers ◽  
Colonization Patterns ◽  
Foot Disease ◽  
Long Acting ◽  
The Uk

Contagious ovine digital dermatitis (CODD) is a severe and common infectious foot disease of sheep and a significant animal welfare issue for the sheep industry in the UK and some European countries. The etiology and pathogenesis of the disease are incompletely understood. In this longitudinal, experimental study, CODD was induced in 18 sheep, and for the first time, the clinical lesion development and associated microbiological changes in CODD affected feet are described over time, resulting in a completely new understanding of the etiopathogenesis of CODD. The majority of CODD lesions (83.9%) arose from pre-existing interdigital dermatitis (ID) and/or footrot (FR) lesions. All stages of foot disease were associated with high levels of poly-bacterial colonization with five pathogens, which were detected by quantitative PCR (qPCR): Treponema medium, Treponema phagedenis, Treponema pedis, Dichelobacter nodosus, and Fusobacterium necrophorum. Temporal colonization patterns showed a trend for early colonization by T. phagedenis, followed by F. necrophorum and D. nodosus, T. medium, and then T. pedis, D. nodosus was present at significantly higher predicted mean log10 genome copy numbers in FR lesions compared to both ID and CODD, while Treponema species were significantly higher in CODD and FR lesions compared to ID lesions (p < 0.001). Treatment of CODD-affected sheep with two doses of 10 mg/kg long acting amoxicillin resulted in a 91.7% clinical cure rate by 3 weeks post-treatment; however, a bacteriological cure was not established for all CODD-affected feet. The study found that in an infected flock, healthy feet, healed CODD feet, and treated CODD feet can be colonized by some or all of the five pathogens associated with CODD and therefore could be a source of continued infection in flocks. The study is an experimental study, and the findings require validation in field CODD cases. However, it does provide a new understanding of the etiopathogenesis of CODD and further supportive evidence for the importance of current advice on the control of CODD; namely, ensuring optimum flock control of footrot and prompt isolation and effective treatment of clinical cases.

scholarly journals Evaluating the Efficiency of Newly Formulated Pomade® and Ceftiofur Hydrochloride for Treating Foot Rot in Dairy Cattle

2021 ◽  
Author(s):  
K. Yiğitarslan ◽  
M. Kale ◽  
D. Öztürk ◽  
N. Mamak
Keyword(s):  
Dairy Cattle ◽  
Economic Loss ◽  
Treatment Method ◽  
Fusobacterium Necrophorum ◽  
Foot Rot ◽  
Chain Reaction ◽  
Dichelobacter Nodosus ◽  
Foot Disease ◽  
New Treatment ◽  
Ceftiofur Hydrochloride

Background: Foot rot is an important contagious disease that causes economic loss in dairy cattle. Even though many antibiotic treatments have been tried on foot rot, very few information about new topical treatment method or product have been reported about the disease. The main objective of this paper is to evaluate an alternative new treatment for foot root disease in dairy cattle. Methods: Forty-one swap samples were collected from cattle’s feet which were further investigated under microscopic examination and polymerase chain reaction (PCR) for Dichelobacter nodosus and Fusobacterium necrophorum. Newly formulated pomade® and ceftiofur hydrochloride (Eficur®) applications were used in foot rot cases. Result: Polymerized chain reaction revealed D. nodosus in all the samples were as F. necrophorum was seen only in 22 (53.66%) samples. In this study, D. nodosus was considered as the primary agent involved in foot disease of cattle and F. necrophorum along with other bacterias were considered to be associated in the infection. The foot rot wounds formed in all cases (100%) in which D. nodosus, F. necrophorum and other bacteria were detected were healed along with tissue regeneration. As a result of treatment applications, a success rate of 93.33% was obtained in lameness resulting due to medium foot rot cases and 45.45% in severe acute lameness. The lameness recovery rate was found to be 80.48%.

scholarly journals Sheep and farm level factors associated with footrot: a longitudinal repeated cross-sectional study of sheep on six farms in the UK

2018 ◽  
Vol 182 (10) ◽  
pp. 293-293 ◽  
Author(s):  
Joseph William Angell ◽  
Dai H Grove-White ◽  
Jennifer S Duncan
Keyword(s):  
Management Practices ◽  
Infectious Agent ◽  
Late Summer ◽  
Risk Groups ◽  
Cross Sectional Study ◽  
Flock Size ◽  
Cross Sectional ◽  
Dichelobacter Nodosus ◽  
Foot Disease ◽  
The Uk

Footrot is an ovine foot disease of infectious origin and a cause of serious welfare and economic compromise in affected animals and flocks. The development of footrot in sheep is associated with the infectious agent Dichelobacter nodosus, which may invade as a primary pathogen, but the risk of disease is increased following damage to the interdigital skin of the foot. In this study, we used data from six farms in North Wales collected between June 2012 and October 2013 to model the dynamic changes of footrot prevalence over time and investigate the association of footrot with multiple farm, management, environmental and sheep factors. Footrot prevalence varied widely within and between farms and overall varied with season with an increase in prevalence shown in late summer and again in the spring. In addition, sheep were more likely to have footrot when the flock size was larger, when grazing poached pasture or when grazing a longer sward, and yearling sheep were less likely to have footrot when compared with lambs and adult sheep. These data may be helpful for advising farmers of likely environmental events, risk groups and management practices that may increase the probability of sheep developing footrot.

scholarly journals Surveying bovine digital dermatitis and non-healing bovine foot lesions for the presence of Fusobacterium necrophorum, Porphyromonas endodontalis and Treponema pallidum

2020 ◽  
Vol 186 (14) ◽  
pp. 450-450 ◽  
Author(s):  
Gareth James Staton ◽  
Leigh Emma Sullivan ◽  
Roger W Blowey ◽  
Stuart D Carter ◽  
Nicholas James Evans
Keyword(s):  
Treponema Pallidum ◽  
Fusobacterium Necrophorum ◽  
Gram Stain ◽  
Digital Dermatitis ◽  
White Line ◽  
Dichelobacter Nodosus ◽  
Lesion Type ◽  
Foot Lesions ◽  
High Level ◽  
Potential Public Health

BackgroundNon-healing bovine foot lesions, including non-healing white line disease, non-healing sole ulcer and toe necrosis, are an increasingly important cause of chronic lameness that are poorly responsive to treatment. Recent studies have demonstrated a high-level association between these non-healing lesions and the Treponema phylogroups implicated in bovine digital dermatitis (BDD). However, a polymicrobial aetiology involving other gram-stain-negative anaerobes is suspected.MethodsA PCR-based bacteriological survey of uncomplicated BDD lesions (n=10) and non-healing bovine foot lesions (n=10) targeting Fusobacterium necrophorum, Porphyromonas endodontalis, Dichelobacter nodosus and Treponema pallidum/T. paraluiscuniculi was performed.ResultsP. endodontalis DNA was detected in 80.0% of the non-healing lesion biopsies (p=<0.001) but was entirely absent from uncomplicated BDD lesion biopsies. When compared to the BDD lesions, F. necrophorum was detected at a higher frequency in the non-healing lesions (33.3% vs 70.0%, respectively), whereas D. nodosus was detected at a lower frequency (55.5% vs 20.0%, respectively). Conversely, T. pallidum/T. paraluiscuniculi DNA was not detected in either lesion type.ConclusionThe data from this pilot study suggest that P. endodontalis and F. necrophorum should be further investigated as potential aetiological agents of non-healing bovine foot lesions. A failure to detect syphilis treponemes in either lesion type is reassuring given the potential public health implications such an infection would present.

scholarly journals Mathematical modeling of ovine footrot in the UK: the effect of Dichelobacter nodosus and Fusobacterium necrophorum on the disease dynamics

Epidemics ◽  
2017 ◽  
Vol 21 ◽  
pp. 13-20 ◽  
Author(s):  
Jolene Atia ◽  
Emma Monaghan ◽  
Jasmeet Kaler ◽  
Kevin Purdy ◽  
Laura Green ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Fusobacterium Necrophorum ◽  
Disease Dynamics ◽  
Dichelobacter Nodosus ◽  
The Uk

scholarly journals The dysbiosis of ovine foot microbiome during the development and treatment of contagious ovine digital dermatitis

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
J. S. Duncan ◽  
J. W. Angell ◽  
P. Richards ◽  
L. Lenzi ◽  
G. J. Staton ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Amplicon Sequencing ◽  
Strong Tendency ◽  
Rrna Gene ◽  
Digital Dermatitis ◽  
Healthy State ◽  
Foot Disease ◽  
End Stage ◽  
And Control ◽  
Interdigital Dermatitis

Abstract Background Contagious Ovine Digital Dermatitis (CODD) is an emerging and common infectious foot disease of sheep which causes severe welfare and economic problems for the sheep industry. The aetiology of the disease is not fully understood and control of the disease is problematic. The aim of this study was to investigate the polybacterial aetiopathogenesis of CODD and the effects of antibiotic treatment, in a longitudinal study of an experimentally induced disease outbreak using a 16S rRNA gene amplicon sequencing approach. Results CODD was induced in 15/30 experimental sheep. During the development of CODD three distinct phenotypic lesion stages were observed. These were an initial interdigital dermatitis (ID) lesion, followed by a footrot (FR) lesion, then finally a CODD lesion. Distinct microbiota were observed for each lesion in terms of microbial diversity, clustering and composition. Porphyromonadaceae, Family XI, Veillonellaceae and Fusobacteriaceae were significantly associated with the diseased feet. Veillonellaceae and Fusobacteriaceae were most associated with the earlier stages of ID and footrot rather than CODD. Following antibiotic treatment of the sheep, the foot microbiota showed a strong tendency to return to the composition of the healthy state. The microbiota composition of CODD lesions collected by swab and biopsy methods were different. In particular, the Spirochaetaceae family were more abundant in samples collected by the biopsy method, suggesting that these bacteria are present in deeper tissues of the diseased foot. Conclusion In this study, CODD presented as part of a spectrum of poly-bacterial foot disease strongly associated with bacterial families Porphyromonadaceae, Family XI (a family in Clostridiales also known as Clostridium cluster XI), Veillonellaceae and Fusobacteriaceae which are predominately Gram-negative anaerobes. Following antibiotic treatment, the microbiome showed a strong tendency to return to the composition of the healthy state. The composition of the healthy foot microbiome does not influence susceptibility to CODD. Based on the data presented here and that CODD appears to be the severest end stage of sheep infectious foot disease lesions, better control of the initial ID and FR lesions would enable better control of CODD and enable better animal welfare.

scholarly journals Systemic glucocorticoid usage in dogs under primary veterinary care in the UK: prevalence and risk factors

2019 ◽  
Vol 185 (4) ◽  
pp. 108-108 ◽  
Author(s):  
Doaa A Elkholly ◽  
Dan O’Neill ◽  
Andrea K Wright ◽  
Kennedy Mwacalimba ◽  
Laura S Nolan ◽  
...  
Keyword(s):  
Primary Care ◽  
Medium Size ◽  
Age Group ◽  
Systemic Glucocorticoid ◽  
Oral Preparation ◽  
Systemic Glucocorticoids ◽  
Study Population ◽  
Long Acting ◽  
The Uk ◽  
To Receive

Glucocorticoids are widely used in primary care veterinary practices. The study aimed to quantify the usage of systemic glucocorticoids (SGC) in dogs in the UK using primary care treatment records recorded during 2013 in the VetCompass Programme. From a study population of 455 557 dogs, 28 472 dogs (6.2 per cent, 95 per cent CI 6.2 to 6.3) received a total of 50 971 SGC therapy events in 2013. Prednisolone represented the most frequently used oral preparation (27 362 events, 90.0 per cent of oral events). Dexamethasone sodium phosphate was the most commonly used injectable agent (12 796 events, 62.7 per cent of injectable events). The most common breed treated was Staffordshire Bull Terriers (2236/28 472 dogs, 7.9 per cent, 95 per cent CI 7.5 to 8.2) and within-breed prevalence of SGC usage was 2236/32 635, 6.9 per cent, 95 per cent CI 6.6 to 7.1. The most commonly treated age group was dogs older than eight years (8931/28472, 31.4 per cent) and the most commonly treated bodyweight group was 10.01–20.0 kg (7918/28 472, 27.8 per cent). Dexamethasone and prednisolone were the most commonly prescribed SGC. Short-acting and intermediate-acting injectable SGC were more commonly used compared with long-acting injectable SGC. Older and medium size dogs were most likely to receive SGC and certain breeds appeared predisposed. These data can provide a useful benchmark for glucocorticoid usage and highlight the benefits from ‘Big Data’ analyses.

scholarly journals Microbiota anaeróbia isolada de bovinos com pododermatite

1999 ◽  
Vol 51 (3) ◽  
pp. 207-212 ◽  
Author(s):  
C.A. Silva ◽  
L.A.F. Silva ◽  
A.J. Mesquita ◽  
M.C.S. Fioravanti ◽  
C.S. Acypreste
Keyword(s):  
Fusobacterium Necrophorum ◽  
Dichelobacter Nodosus

O presente trabalho teve como objetivo isolar e identificar espécies bacterianas anaeróbias presentes nos pés de bovinos portadores de vários graus de pododermatite. Foram utilizados 60 bovinos, distribuídos em quatro grupos de 15. O grupo I foi constituído por animais saudáveis e serviu de controle; o grupo II, por bovinos na fase inicial do processo; o grupo III, por animais portadores de pododermatite interdigital vegetativa e o grupo IV, por bovinos portadores de pododermatite necrosante. Foram colhidos fragmentos de tecido interdigital para cultura e as principais espécies bacterianas isoladas foram: Dichelobacter nodosus nos grupos II, III e IV e Fusobacterium necrophorum nos grupos III e IV, com freqüências de 26,7%, 6,7%, 20,0%, 6,7% e de 13,3%, respectivamente. Encontraram-se também Fusobacterium symbiosum em 40,0% no gb>rupo I, 6,7% no grupo II, 13,3% no grupo III e 13,3% no grupo IV, Bacteroides sp. em 6,7% nos grupos I e IV, Bacteroides ruminatus em 33,3% no grupo I, 6,7% no grupo II, 33,3% no grupo III e 13,3% no grupo IV, Bacteroides oralis em 6,7% no grupo III e Fusobacterium mortiferum em 6,7% no grupo IV.

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