Antioxidant potentials of Terminalia catappa leaf extractin Streptozotocin induced diabetes in rats

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.4552 ◽

2016 ◽

Author(s):

Natarajan Divya ◽

Arumugam Vijaya Anand

Keyword(s):

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Ethanolic Extract ◽

Serum Levels ◽

Albino Rats ◽

Low Density ◽

Subsequent Increase ◽

Terminalia Catappa ◽

Percolation Method

The objective of this study to evaluate the antioxidant capacity of the ethanolic extract of Terminalia catappa in vivo in Wistar albino rats. Streptozotocin (STZ) used as toxin it induces diabetes, damages the cell membrane and causes oxidative stress. The leaves (T. catappa) were extracted using 95 % ethanol by hot continuous percolation method. The extracts were concentrated by rotaevaporador. The residues extract were administrated orally to the STZ induced diabetes animals. After 45th day the animals were sacrificed and blood, liver tissues were collected and various antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), were reduced in the STZ alone treated animals with subsequent increase in LPO. The serum levels of total cholesterol (TC), triglycerides (TG), free fatty acids (FFA), phospholipids, low density lipoprotein (LDL), very low density lipoprotein (VLDL), and high density lipoprotein (HDL) was found out. Treatment with ethanolic extract of T. catappa at a dose of 300, 500 mg/kg once in a day has altered the levels of biochemical markers and brings back to near normal levels. Among which the dose 500 mg/kg having scavenging action to eradicate free radicals and maintained antioxidant status. The statistical data of P Values less than 0.001 were considered as level of significance.

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ANTIDIABETIC AND ANTIHYPERLIPIDEMIC ACTIVITY OF DRACAENA CINNABARI BALF. RESIN ETHANOLIC EXTRACT OF SOQATRA ISLAND IN EXPERIMENTAL ANIMALS

Universal Journal of Pharmaceutical Research ◽

10.22270/ujpr.v3i5.194 ◽

2018 ◽

Author(s):

Nahed Al-Baoqai ◽

Hassan Al-Mahbashi ◽

Adnan Al-Adhal

Keyword(s):

Low Density Lipoprotein ◽

Fasting Blood Glucose ◽

Density Lipoprotein ◽

Ethanolic Extract ◽

Albino Rats ◽

Low Density ◽

Experimental Animals ◽

Socotra Island ◽

Antihyperlipidemic Activity ◽

Dracaena Cinnabari

Dracaena Cinnabari balfis a species plant in Abalfurn 1984e de la p pharmaciegavaceae family. It is a tree endemic to the Island of Socotra,Yemen. It was used as a dye and medicine in Socotra and the Mediterranean basin. Some researches demonstrated both hypoglycemic and hypolipidemic of its resin of Dracaena Cochinchinensis, a traditional Chinese tree. Accordingly, this study aimed to evaluate the antidiabetic potential of the ethanolic extract of dracanea cinnabari balfresin (DCBR), endemic in Socotra Island, in Alloxan induced diabetes, and evaluation of possible antihyperlipidemic activity of the same extract in high-cholesterol diet-induced hyperlipidemia in male albino rats. Experimental diabetes was induced in rats with intraperitoneal injection a single dose of Alloxan (150 mg/kg). Hyperlipidemia was induced in rats by administration of 1% cholesterol in diet and 0.5 % Hydrogen Peroxide in drinking water for 14 days. Fasting blood glucose levels were measured at 1st day, 7th day and 14th day. Pancreas histopathology was done at the end. Estimation of serum lipid profile and liver histopathology was done at the end of 14 day. The results were expressed as mean ± SD and differences among the groups of animals were compared using one-way ANOVA. Administration of ethanolic extract of resin of two doses (100mg and 300mg\kg) in alloxan induce diabetic rats resulted in a significant decrease in FBG levels with a recovery in destruction of pancreas cell compared with untreated group .Also the same doses of extract showed significant decrease in triglycerides, total cholesterol, low density lipoprotein, very low density lipoprotein and alkaline phospatase in comparing with untreated groups. From the results of present study we can conclude the ethanolic extract of Dracaena Cinnabari Balf. have a hypoglycemic and hypolipidemic activity in experimental animals , these activity are time and dose dependent.

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Beneficial effects of melatonin and alpha lipoic acid on lopinavir/ ritonavir-induced alterations in serum lipid and glucose levels of male albino rats

Macedonian Pharmaceutical Bulletin ◽

10.33320/maced.pharm.bull.2016.62.01.004 ◽

2016 ◽

Vol 62 (1) ◽

pp. 47-55 ◽

Cited By ~ 1

Author(s):

Elias Adikwu ◽

Brambaifa Nelson ◽

Wolfe Atuboyedia Obianime

Keyword(s):

Serum Lipid ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Serum Levels ◽

Albino Rats ◽

Placebo Control ◽

Low Density ◽

Alpha Lipoic Acid ◽

Low Density Lipoprotein Cholesterol ◽

Glucose Levels

The use of lopinavir/ritonavir (LPV/r) has been associated with alterations in serum lipid and glucose levels. This study was designed to investigate the effects of melatonin (MT) and alpha lipoic acid (ALA) on LPV/r-induced changes in serum levels of triglycerides (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C) and glucose (GL) levels in male albino rats. Rats in group A1 (placebo control) and group A2 (solvent control) were orally treated with normal saline and 1% ethanol respectively. Groups B-F contained 15 rats each which were divided into 3 groups of 5 rats each. Rats in group B were orally treated with MT (10 mg kg-1/day), ALA (10 mg kg-1/day) and a combination of MT and ALA, accordingly. Rats in group C were orally treated with 22.9/5.71, 45.6/11.4 and 91.4/22.9 mg kg-1/day of LPV/r, accordingly. Rats in groups D-F were orally treated with MT (10 mg kg-1/ day), ALA (10 mg kg-1/day) and combined doses of ALA and MT before oral treatment with 22.9/5.71, 45.6/11.4 and 91.4/22.9 mg kg-1/day of LPV/r, accordingly. All rats were treated for 30 days and at the end of the drug treatment, the serum levels of lipid fractions and glucose were evaluated. Treatment with MT and ALA significantly (p<0.05) decreased baseline serum levels of TG, TC, VLDL-C, LDL-C and GL, but these parameters were significantly (p<0.05) increased in a dose-dependent manner in LPV/r-treated rats when compared to placebo control. Administration of MT and ALA prior to treatment with LPV/r significantly (p<0.05) decreased serum levels of TG, TC, VLDL-C, LDL-C and GL when compared to LPV/r-treated rats. However, decreases obtained in rats pretreated with combined doses of MT and ALA were significantly (p<0.05) different when compared to their individual doses. This study showed that MT and ALA can serve as remedies for LPV/r-induced alterations in serum lipid and glucose levels.

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Hypolipidemic activity of ethanolic extract of Caulerpa recemosa

BIRDEM Medical Journal ◽

10.3329/birdem.v9i3.43080 ◽

2019 ◽

Vol 9 (3) ◽

pp. 197-201

Author(s):

Sayeeda Monira Rahman ◽

Sharif Neaz ◽

Md Morshed Alam ◽

Jasmin Nur

Keyword(s):

Biochemical Parameters ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Serum Levels ◽

Atherogenic Index ◽

Hypolipidemic Activity ◽

Low Density ◽

Caulerpa Racemosa

Background: Caulerpa recemosa, also known as sea grape, is a stout straggling prostrate shrubby plant with spinous stipules and uniaxial siphonous thallus, mostly divided into a creeping axis (stolon), with rhizoids and erect shoots (fronds), either nude, leaf-like or with grape or feather-like ramuli. Caulerpa racemosa has erect fronds up to 9-13 cm high bearing un-crowded vesiculate ramuli that are radially arranged. Fronds are slightly inflated above the attachment to the stolon which are fixed to the substrate by thin short rhizoids. It is collected from St. Martin’s Island locally called ‘Narikel Jinjira’, located on the southernmost tip of Bangladesh for the present study, ethanol extract of Caulerpa recemosa has been screened for its hypolipidemic activity. Methods: Hypolipidemic activity was screened by inducing hyperlipidemia with the help of atherogenic diet in wistar albino rats and serum levels of various biochemical parameters such as total cholesterol, triglycerides, low density lipoprotein and high density lipoprotein cholesterol were determined. Atherogenic index shows the measure of the atherogenic potential of the drugs. Results: Ethanol extract showed significant (p < 0.01) hypolipidemic effect by lowering the serum levels of biochemical parameters such as significant reduction in the level of serum cholesterol, triglyceride, low density lipoprotein and increase in high density lipoprotein level which was similar to the standard drug atorvastatin. Ethanol extract exhibited significant atherogenic index andpercentage protection against hyperlipidemia. Preliminary phytochemical analysis revealed the presence of phytoconstituents such as steroids, flavonoids, glycosides, alkaloids, phenoliccompounds. Conclusion: The overall experimental results suggest that the biologically active phytoconstituents such as flavonoids, glycosides alkaloids present in the ethanolic extract of Caulerpa racemosa may be responsible for the significant hypolipidemic activity and the results may justify the use of Caulerpa racemosa as a significant hypolipidemic agent. Birdem Med J 2019; 9(3): 197-201

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Overexpression of miR-29a-3p Suppresses Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells in Atherosclerosis via Targeting TNFRSF1A

BioMed Research International ◽

10.1155/2020/9627974 ◽

2020 ◽

Vol 2020 ◽

pp. 1-15

Author(s):

Liyi You ◽

Hao Chen ◽

Lixin Xu ◽

Xun Li

Keyword(s):

Smooth Muscle ◽

Vascular Smooth Muscle Cells ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Serum Levels ◽

Plaque Formation ◽

Migration And Invasion ◽

Low Density

Objective. Increasing evidence highlights the significance of microRNAs (miRNAs) in the progression of atherosclerosis (AS). Our aim was to probe out the role and regulatory mechanism of miR-29a-3p in AS. Methods. An in vivo model of AS was conducted by high-fat diet ApoE-/- mice. Oxidized low-density lipoprotein- (ox-LDL-) exposed vascular smooth muscle cells (VSMCs) were utilized as an in vitro of AS. Serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were detected. Hematoxylin and eosin (H&E) and Masson’s staining was presented to investigate the pathological changes. miR-29a-3p and TNFRSF1A expression was detected by RT-qPCR. Proliferative, migrated, and invaded abilities of VSMCs were determined via a series of assays. The interaction between miR-29a-3p and TNFRSF1A was verified through luciferase reporter assay. Results. Upregulated miR-29a-3p and downregulated TNFRSF1A were found both in vitro and in vivo models of AS. miR-29a-3p mimic distinctly decreased the serum levels of TC, TG, and LDL-C and increased serum HDL-C levels. Moreover, its overexpression could ameliorate plaque formation of AS mice. In ox-LDL-induced VSMCs, miR-29a-3p overexpression notably decreased cell proliferation, migration, and invasion, which was reversed by TNFRSF1A overexpression. Also, miR-29a-3p could directly target the 3′UTR of TNFRSF1A. Conclusion. miR-29a-3p overexpression ameliorated plaque formation of AS and suppressed proliferation, migration, and invasion of ox-LDL-induced VSMCs via TNFRSF1A, which offered novel insights into the progression of AS.

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Oxidation of Plasma Low-Density Lipoprotein Accelerates Its Accumulation and Degradation in the Arterial Wall In Vivo

Circulation ◽

10.1161/01.cir.94.7.1698 ◽

1996 ◽

Vol 94 (7) ◽

pp. 1698-1704 ◽

Cited By ~ 46

Author(s):

Klaus Juul ◽

Lars B. Nielsen ◽

Klaus Munkholm ◽

Steen Stender ◽

Børge G. Nordestgaard

Keyword(s):

Arterial Wall ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density

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Interaction of 4-hydroxynonenal-modified low-density lipoproteins with the fibroblast apolipoprotein B/E receptor

Biochemical Journal ◽

10.1042/bj2340245 ◽

1986 ◽

Vol 234 (1) ◽

pp. 245-248 ◽

Cited By ~ 31

Author(s):

W Jessup ◽

G Jurgens ◽

J Lang ◽

H Esterbauer ◽

R T Dean

Keyword(s):

Apolipoprotein B ◽

Negative Charge ◽

Low Density Lipoprotein ◽

Ldl Receptor ◽

Density Lipoprotein ◽

Low Density ◽

Lipid Peroxidation Product ◽

Modified Low Density Lipoproteins ◽

Peroxidation Product

The incorporation of the lipid peroxidation product 4-hydroxynonenal into low-density lipoprotein (LDL) increases the negative charge of the particle, and decreases its affinity for the fibroblast LDL receptor. It is suggested that this modification may occur in vivo, and might promote atherogenesis.

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