scholarly journals Analisis Sidik Jari Kromatografi Lapis Tipis Tanaman Anting-Anting (Acalypha indica L.)

ALCHEMY ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 54-62
Author(s):  
Muhammad Teguh Laksono ◽  
Elok Kamilah Hayati
Keyword(s):  
Chemical Compounds ◽  
Raw Material ◽  
Fingerprint Analysis ◽  
Chromatography Method ◽  
Chromatographic Profile ◽  
Dragendorff Reagent ◽  
The Stability ◽  
Tlc Analysis ◽  
Layer Chromatography ◽  
Acalypha Indica

Anting-anting plant (Acalypha indica L.) is a plant that the community has used as raw material for herbal medicine. Anting-anting plants contain chemical compounds, so it is difficult to ensure these plants' safety and quality control. The research objective was to validate the method by knowing the stability, specificity, precision, and rigidity of the chromatographic profile of alkaloid compounds in the TLC analysis of the anting-anting extract. Extraction was carried out using ethyl acetate solvent for 20 minutes with a frequency of 42 kHz. The eluent used is cyclohexane: toluene: diethylamine (75:15:10) with Dragendorff reagent. The method of validation was acceptable according to the criteria except for the stability test of the analyte during chromatography. The instability of the analyte is indicated that not all spots form a straight diagonal line. The specificity of the method was carried out by comparing the fingerprint patterns of the leaves of the anting-anting with ekor kucing (Acalypha hibsida Farm.) and teh-tehan plants (Acalypha siamensis). The fingerprint profile showed a yellow stain (Rf = 0.39) on the leaves of the anting-anting, which did not appear in the fingerprint profiles of the two comparison samples. Keywords: anting-anting, fingerprint analysis, thin layer chromatography, method validation Anting-anting (Acalypha indica L.) merupakan tanaman yang telah dimanfaatkan masyarakat sebagai bahan baku obat herbal. Anting-anting mengandung banyak senyawa kimia sehingga sulit menjamin keamanan dan pengendalian mutu dari tanaman tersebut. Penelitian ini dilakukan untuk validasi metode dengan mengetahui kestabilan, spesifitas, presisi, dan ketegaran profil kromatografi senyawa alkaloid pada analisis KLT ekstrak anting-anting. Ekstraksi dilakukan menggunakan pelarut etil asetat selama 20 menit dengan frekuensi 42 kHz. Eluen yang digunakan adalah sikloheksana : toluena : dietilamin (75:15:10) dengan reagen Dragendorff. Validasi metode dapat diterima sesuai kriteria kecuali uji stabilitas analit selama kromatografi. Ketidakstabilan analit ditunjukkan dengan seluruh noda yang dihasilkan tidak membentuk garis diagonal yang lurus. Spesifitas pada metode dilakukan dengan membandingkan pola sidik jari daun anting-anting dengan daun ekor kucing (Acalypha hibsida Farm) dan daun teh-tehan (Acalypha siamensis). Profil sidik jari menunjukkan bahwa terdapat noda berwarna kuning kehijauan (Rf = 0,39) pada daun anting-anting yang tidak muncul pada profil sidik jari kedua sampel pembanding tersebut. Kata kunci: anting-anting, analisis sidik jari, kromatografi lapis tipis, validasi metode

scholarly journals DIFFERENTIATION OF Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar BY THIN LAYER CHROMATOGRAPHY FINGERPRINT ANALYSIS

2011 ◽  
Vol 11 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Mohamad Rafi ◽  
Eti Rohaeti ◽  
Ali Miftahudin ◽  
Latifah K. Darusman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Curcuma Longa ◽  
Chemical Compounds ◽  
Specific Marker ◽  
Fingerprint Analysis ◽  
Fingerprint Pattern ◽  
Curcuma Xanthorrhiza ◽  
Zingiber Cassumunar ◽  
Layer Chromatography

Turmeric (Curcuma longa), java turmeric (Curcuma xanthorrhiza) and cassumunar ginger (Zingiber cassumunar) are widely used in traditional Indonesian medicine. These three herbs have relatively similar rhizomes colour so it is difficult to be differentiated especially if they are in powder form. A rapid and reliable method, thin layer chromatography (TLC) fingerprint, has been developed in order to identify, authenticate and differentiate these three herbs through fingerprint profile of chemical compounds. TLC fingerprints of the three herbs were obtained by visualization of separate zones with visible and UV (254 and 366 nm) light. The TLC fingerprint pattern is different each other and showed a specific marker zones respectively. Therefore, TLC fingerprint can be utilized for identification, authentication and differentiation method in quality control of the three herbs tested.

scholarly journals Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus

2019 ◽  
Vol 7 (22) ◽  
pp. 3882-3886
Author(s):  
Yessi Febriani ◽  
Vriezka Mierza ◽  
Novi Putri Handayani ◽  
Surismayanti Surismayanti ◽  
Ibrenaita Ginting
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Chromatography Method ◽  
E Coli ◽  
Layer Chromatography ◽  
And Staphylococcus Aureus

BACKGROUND: Infection is the most common diseases in developing country, including Indonesia. Bacteria that often causes infection is Escherichia coli and Staphylococcus aureus. One of the traditional plants that can be used as an antibacterial is lidah mertua. AIM: The purpose of this study was to find out the profile of chemical compounds by thin layer chromatography method and determine the antibacterial activity of Lidah Mertua leaves by in vitro. METHODS: This research conducted an experimentally using non-polar, semipolar, and polar as solvents to get extract against E. coli and S. aureus as bacterial testing. The antibacterial activity using agar diffusion method to get minimum inhibitory concentration (MIC). RESULTS: The result of the research on thin layer chromatography showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The data obtained were tabulated and analysed descriptively. The antibacterial activity show that n-hexane extract does not provide inhibitory activity. MIC value show that aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 50 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.50 mm and 8.20 mm and methanol extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 12.5 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.46 mm and 8.32 mm. CONCLUSION: The profile of chemical compounds by thin layer chromatography method showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The antibacterial activity show that n-hexane extract does not provide inhibitory activity, but aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus.

scholarly journals Development of Validated Stability-indicating High Performance Thin Layer Chromatography Method for Estimation of Rabeprazole Sodium and Aceclofenac in Bulk Drug

2021 ◽  
pp. 168-185
Author(s):  
Sunil Shewale ◽  
Vaishali Undale ◽  
Maruti Shelar ◽  
Vrushali Bhalchim ◽  
Atul Deshmukh
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chromatography Method ◽  
Stability Indicating ◽  
Thin Layer Chromatography Method ◽  
Rabeprazole Sodium ◽  
The Stability ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: To develop a validated stability-indicating high performance thin layer chromatography method for the estimation of Rabeprazole Sodium (RZL) and Aceclofenac (ACF) in bulk drugs. Methods: A high performance thin layer chromatographic (HPTLC) method has been developed for the separation of RZL & ACF on plates precoated with aluminium back silica gel 60 F254. Different mobile phases were used on trial and error basis for separation of two drugs. The final mobile phase selected for analysis was toluene: ethyl acetate: methanol: acetic acid: ammonia in the ration of 6:4:1:0.2:0.1 (v/v). Both the drugs showed maximum absorbance at 279 nm which was selected as the detection wavelength throughout the experimental work. Developed method was validated as per ICH guidelines. Forced degradation of drugs was carried out under various stress conditions and HPTLC method was used for analysing the stability of drugs. Results: HPTLC method was successfully developed for separation of RZL and ACF with clear separation of bands of the drugs. Method validation after assessment of various parameters indicated low % RSD within an acceptable limit of < 2.0 and the stability studies indicated the satisfactory separation of both the drugs from that of degraded products with considerable % recovery profile. Conclusion: The developed method is rapid, reliable, precise, and reproducible and demonstrates the suitability of the method for stability determination of rabeprazole and aceclofenac.

scholarly journals Identification and quantitative determination of the flavonoids of the complex dense extract of st. john's wort herb and pot marigold flowers

2018 ◽  
pp. 71-79
Author(s):  
T. A. Shostak ◽  
T. G. Kalyniuk ◽  
L.V. Vronska
Keyword(s):  
Active Pharmaceutical Ingredient ◽  
Biologically Active ◽  
Chromatography Method ◽  
St John’S Wort ◽  
St John's Wort ◽  
Chromatographic Profile ◽  
Pot Marigold ◽  
Layer Chromatography

Common Saint-John's wort (Hypericum perforatum) and pot marigold (Calendula officinalis) are rich in such biologically active substances (BAS) as carotene, ascorbic acid, essential oils, vitamins, tannin and resinous substances, as well as flavonoids that bear evident wound healing properties and antiulcerous properties. The object of this study was BAR composition of the complex dense herb extract of St. John's wort and flowers of marigolds (1:10). In order to introduce a new herbal substance into medical practice, it is necessary to develop methods for its identification and quantification. The TLC [thin layer chromatography] method was used to identify the BAR in the extract under study, and the method of absorption spectrophotometry was proposed for quantification of the content of flavonoids. As a result of the conducted research, there were selected characteristic substances - identification markers of the extract, the choice of which was in accordance with the requirements of the SPF on the quality of the herb of St. John's wort and the flowers of pot marigold, and there was indicated the position and coloring of the zones in the chromatographic profile of the tested extract solution. Such approach will enable objective identification of the extract as a substance and as an active pharmaceutical ingredient in the formulation. The criterion for quantitative standardization of the complex dense extract is the content of the amount of flavonoids not less than 1.5% in terms of hyperoside and dry substance.

scholarly journals ISOLATION AND DETERMINATION CARPAINE ALKALOID IN PAPAYA (CARICA PAPAYA L.) LEAF EXTRACT BY THIN-LAYER CHROMATOGRAPHY SCANNER

2021 ◽  
pp. 234-238
Author(s):  
YASMIWAR SUSILAWATI ◽  
NYI MEKAR SAPTARINI ◽  
ZELIKA MEGA RAMADHANIA ◽  
ELI HALIMAH ◽  
NAILAH NURJIHAN ULFAH
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Limit Of Detection ◽  
Preparative Thin Layer Chromatography ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Dragendorff Reagent ◽  
Layer Chromatography

Objective: This research was conducted to isolate the alkaloid carpaine by chromatography method and to determine it quantitatively by Thin Layer Chromatography Scanner. Methods: Dried leaves were macerated with ethanol 70% and fractionated with dichloromethane. Isolation of carpaine alkaloid from the dichloromethane fraction was carried out by column chromatography and preparative thin-layer chromatography according to the Rf value in Thin Layer Chromatography (TLC) after exposure by Dragendorff reagent. Results: The content of carpaine alkaloid was 7.5 mg with Rf 0.58 and dichloromethane: methanol (9.2:0.8) as eluent. Validation showed the linearity (R2) 0.9988, the limit of detection(LOD) was 0.05 ppm, the Limit of Quantification (LOQ) was 0.19 ppm, the recovery from 98.93-102.43%, and the % coefficient of variation was 0.16%. Conclusion: Carpaine alkaloid in papaya leaf extract was 10.52%.

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