Screening of probiotic bacteria and its role on artificial infection of Vibrio harvey in white shrimp (Litopenaeus vannamei)

Probiotic was screened from 28 strains of normal bacterial flora isolated from rearing water in a Litopenaeus vannamei farm based on its inhibitory activity against the growth of Vibrio harveyi. Antibacterial activity was also tested in vivo to V. harveyi in L. vannamei. The result showed that the probiotic has a antibacterial effect on V. harveyi. The in vivo test showed that shrimps injected with probiotic previously before challenged with V. harveyi has survival higher than control. Probiotic isolate was suspected as Vibrio furnissi. Keywords: biocontrol, inhibitory activity, Vibrio furnissi, Vibrio harveyi, Litopenaeus vannamei AbstraK Bakteri probiotik ditapis dari 28 strain bacteria flora yang diisolasi dari air pemeliharaan udang vaname Litopenaeus vannamei berdasarkan aktivitas penghambatannya terhadap pertumbuhan Vibrio harveyi. Aktivitas bakteri probiotik juga diuji secara in vivo terhadap V. harveyi pada udang putih. Hasil penelitian menunjukkan bahwa bakteri probiotik isolat memiliki kemampuan antibakteria terhadap V. harveyi. Uji in vivo menunjukkan bahwa udang yang diinjeksi probiotik sebelum diuji tantang dengan V. harveyi memiliki kelangsungan hidup lebih tinggi daripada kontrol. Isolat probiotik tersebut diduga adalah Vibrio furnissi. Kata kunci: biokontrol, aktivitas penghambatan, Vibrio furnissi, Vibrio harveyi, Litopenaeus vannamei

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Inhibitory activity of native probiotic Bacillus vallismortis IS03 against pathogenic Vibrio harveyi under in vitro and in vivo conditions in Litopenaeus vannamei

Iranian Journal of Aquatic Animal Health ◽

10.29252/ijaah.5.2.54 ◽

2019 ◽

Vol 5 (2) ◽

pp. 54-66

Author(s):

M Mahjoub ◽

M Mirbakhsh ◽

M Afsharnasab ◽

S Kakoolaki ◽

S Hosseinzadeh ◽

...

Keyword(s):

Litopenaeus Vannamei ◽

Inhibitory Activity ◽

Vibrio Harveyi ◽

Pathogenic Vibrio ◽

Bacillus Vallismortis

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Use of Chitosan to Control Vibrio harveyi Infection on White Shrimp Litopeneaus vannamei

Jurnal Akuakultur Indonesia ◽

10.19027/jai.6.205-209 ◽

2007 ◽

Vol 6 (2) ◽

pp. 205

Author(s):

. Sukenda ◽

Y. Tri Anggoro ◽

D. Wahjuningrum ◽

. Rahman

Keyword(s):

Antibacterial Activity ◽

Survival Rate ◽

Litopenaeus Vannamei ◽

Antibacterial Effect ◽

Sea Water ◽

Vibrio Harveyi ◽

White Shrimp ◽

Phagocytic Index ◽

Post Injection ◽

Total Haemocyte Count

Immunostimulation and antibacterial effect of chitosan against vibriosis were examined in white shrimp (Litopenaeus vannamei). Control shrimps were injected with 0.05 μl of sterile sea water, while experimental shrimps were injected with 2, 4 and 6 μg per g shrimp of chitosan. All shrimps were subsequently challenged by 106 CFU/ml of live Vibrio harveyi by injection method. Survival rate of shrimps injected with chitosan were found to slightly increase in accordance with dose of chitosan, even not statistically significant. Total haemocyte count and phagocytic index at experimental shrimps were over than control shrimps up to three days post injection. Number of V. harveyi in the intestine of experimental shrimps were lower than control shrimps indicates an antibacterial activity of chitosan to combat infection. Keywords: chitosan, Vibrio harveyi, haemocyte, phagocytic index, Litopenaeus vannamei ABSTRAK Efek imunostimulasi dan antibakterial dari kitosan melawan vibriosis dilihat pada udang putih (Litopenaeus vannamei). Udang control disuntik dengan 0,05 μl air laut steril, sedangkan udang uji disuntik dengan kitosan 2, 4 dan 6 μg per g udang. Semua udang diuji tantang dengan 106 CFU/ml bakteri Vibrio harveyi hidup dengan metode penyuntikan. Sintasan udang yang disuntik dengan kitosan meningkat berbarengan dengan peningkatan dosis kitosan, meskipun tidak menunjukkan perbedaan yang nyata. Jumlah total hemosit dan indeks fagositosis pada udang lebih tinggi dibandingkan kontrol sampai tiga hari pasca penyuntikan. Jumlah V. harveyi dalam saluran pencernaan dari udang uji lebih rendah dibandingkan udang kontrol, hal ini menunjukkan aktifitas antibacterial dari kitosan dalam melawan infeksi. Kata kunci: kitosan, Vibrio harveyi, hemosit, indeks fagositosis, Litopenaeus vannamei

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Draft Genome Sequence of a Vibrio harveyi Strain Associated with Vibriosis in Pacific White Shrimp (Litopenaeus vannamei)

Genome Announcements ◽

10.1128/genomea.01662-16 ◽

2017 ◽

Vol 5 (7) ◽

Cited By ~ 5

Author(s):

Emille Moreno ◽

Marci Parks ◽

Lee J. Pinnell ◽

James J. Tallman ◽

Jeffrey W. Turner

Keyword(s):

Genome Sequence ◽

Litopenaeus Vannamei ◽

Vibrio Harveyi ◽

Draft Genome ◽

Penaeid Shrimp ◽

Pacific White Shrimp ◽

Draft Genome Sequence ◽

White Shrimp ◽

Content Type ◽

Future Studies

ABSTRACT Vibrio harveyi is a Gram-negative bacterium associated with vibriosis in penaeid shrimp. Here, we report the draft genome sequence of a V. harveyi strain isolated from Pacific white shrimp (Litopenaeus vannamei) during a vibriosis outbreak. The availability of this genome will aid future studies of vibriosis in shrimp aquaculture.

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