scholarly journals ANTIBIOTIC SUSCEPTIBILITY IN UROPATHOGEN FROM INTENSIVE CARE PATIENTS WITH URINE CATHETER

Medicinus ◽  
2018 ◽  
Vol 4 (9) ◽  
Author(s):  
Angeline Nifiani M Wibowo ◽  
Cucunawangsih Cucunawangsih
Keyword(s):  
Escherichia Coli ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Species Identification ◽  
Urine Culture ◽  
Multi Drug Resistance ◽  
Intensive Care Patients ◽  
Gram Positive Bacteria ◽  
Antibiotics Susceptibility ◽  
One Year

<p><strong><em>Background</em></strong><em>: Multi-Drug Resistance Organisms (MDROs) are defined as organisms that acquired non-susceptibility to more than one antimicrobial agent. Intensive care patients are immune-compromised patients, using catheter and are given broad-spectrum antibiotics. Hence, the chance to develop microbial resistance is high. The aim of this study is to see the etiology and the microbial susceptibility pattern of catheter-associated urinary tract infection patients treated in intensive care.</em></p><p><strong><em>Materials and Methods</em></strong><em>: The urine samples were taken from catheterized patients admitted to intensive care in Siloam Lippo Village, Tangerang, Indonesia in a one year period from July 2013 until June 2014. We confirmed species identification with Vitex-2 Compact<sup>®</sup> from Biomérieux, France. The susceptibility of antibiotics is according to Clinical and Laboratory Standard Institute (CLSI). </em></p><p><strong><em>Results</em></strong><em>: We managed to get 113 urine culture results with mean of age 57.03 ± 18.505 (years). There were 67 males (59.3%) and 46 females (40.70%) that were acquired in the sample. The result of species identification showed that Escherichia coli was the dominant isolate from the urine culture (40.63%), followed by Klebsiella pneumoniae (12.5%). The percentage of MDRO was found to be 71.9%. The antibiotics susceptibility of Escherichia coli for Amoxicilin, Ampicillin/Sulbactam, Ciprofloxacin and Levofloxacin are 50%, 58%, 76% and 75% respectively. Meanwhile the susceptibility against Amikacin and Meropenem are 100% for Escherichia coli and Klebsiella pneumoniae. </em></p><p><strong><em>Conclusion</em></strong><em>: The proportion of Escherichia coli was the highest among with susceptibility of Meropenem was still high susceptibility for both gram negative and gram positive bacteria. </em></p><h2> </h2>

Acquisition of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in intensive care units in Thailand

2020 ◽  
Author(s):  
Kanit Assawatheptawee ◽  
Anong Kiddee ◽  
Anamai Na-udom ◽  
Apirath Wangteeraprasert ◽  
Pornpit Treebupachatsakul ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Intensive Care Units ◽  
Extended Spectrum

scholarly journals Emergence of Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli Isolates Possessing the Plasmid-Mediated Carbapenem-Hydrolyzing β-Lactamase KPC-2 in Intensive Care Units of a Chinese Hospital

2008 ◽  
Vol 52 (6) ◽  
pp. 2014-2018 ◽  
Author(s):  
Jia Chang Cai ◽  
Hong Wei Zhou ◽  
Rong Zhang ◽  
Gong-Xiang Chen
Keyword(s):  
Escherichia Coli ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Serratia Marcescens ◽  
Intensive Care Units ◽  
Gel Electrophoresis ◽  
Outer Membrane Proteins ◽  
Carbapenem Resistance ◽  
E Coli ◽  
Insertional Inactivation

ABSTRACT Twenty-one Serratia marcescens, ten Klebsiella pneumoniae, and one Escherichia coli isolate with carbapenem resistance or reduced carbapenem susceptibility were recovered from intensive care units (ICUs) in our hospital. Enterobacterial repetitive intergenic consensus-PCR and pulsed-field gel electrophoresis demonstrated that all the S. marcescens isolates belonged to a clonal strain and the 10 K. pneumoniae isolates were indistinguishable or closely related to each other. The MICs of imipenem, meropenem, and ertapenem for all isolates were 2 to 8 μg/ml, except for K. pneumoniae K10 (MICs of 128, 256, and >256 μg/ml). Isoelectric focusing, PCRs, and DNA sequencing indicated that all S. marcescens isolates produced KPC-2 and a β-lactamase with a pI of 6.5. All K. pneumoniae isolates produced TEM-1, KPC-2, CTX-M-14, and a β-lactamase with a pI of 7.3. The E. coli E1 isolate produced KPC-2, CTX-M-15, and a β-lactamase with a pI of 7.3. Conjugation studies with E. coli (EC600) resulted in the transfer of reduced carbapenem susceptibility compared to that of the original isolates, and only the bla KPC-2 gene was detected in E. coli transconjugants. Plasmid restriction analysis showed identical restriction patterns among all E. coli transconjugants. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and ompK35/36 gene sequence analysis of outer membrane proteins revealed that K. pneumoniae K10 failed to express OmpK36, because of insertional inactivation by an insertion sequence ISEcp1. All these results indicate that KPC-2-producing S. marcescens, K. pneumoniae, and E. coli isolates emerged in ICUs in our hospital. KPC-2 combined with porin deficiency results in high-level carbapenem resistance in K. pneumoniae. The same bla KPC-2-encoding plasmid was spread among the three different genera.

scholarly journals KARAKTERISTIK ANTIBIOGRAM ISOLAT Escherichia coli DAN Klebsiella pneumoniae DARI RUANG INTENSIVE CARE UNIT DAN NON-INTENSIVE CARE UNIT DI RSUP SANGLAH SELAMA TAHUN 2018-2020

2021 ◽  
Vol 7 (1) ◽  
pp. 8
Author(s):  
Ni Luh Ranthi Kurniawathi ◽  
Indramawan Setyojatmiko ◽  
Ni Nyoman Sri Budayanti
Keyword(s):  
Escherichia Coli ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Beta Lactamases ◽  
E Coli ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases

Resistesi antibiotik meningkat secara global dalam beberapa tahun ini, terutama kejadian Escherichia coli (E.coli) dan Klebsiella pneumoniae (K.pneumoniae) penghasil Extended Spektrum Beta Lactamases (ESBL). Tujuan dari penelitian ini adalah untuk memberikan gambaran prevalensi keberadaan dan antibiogram isolat E.coli dan K. pneumoniae penghasil ESBL di rumah sakit tersier di Bali. Penelitian retrospektif potong lintang ini dlikaukan pada Januari 2018- Desember 2020 di Rumah Sakit Umum Pusat Sanglah, Bali. Identifikasi bakteri dan uji sensitivitas antibiotik dilakukan dengan alat otomatis Vitek®2 Compact. Hasil penelitian menunjukkan dari 2972 isolat, 1067 (63,82%) isolat adalah E. coli penghasil ESBL dan 902 isolat (69,39%) adalah K. pneumoniae penghasil ESBL. Isolat penghasil ESBL ditemukan terbanyak pada non-ICU (89,39%). Bakteri E.coli penghasil ESBL menunjukkan sensitivitas > 80% terhadap Amikacin, Ertapenem, Meropenem, Nitrofurantoin, Piperacillin-tazobactam, dan Tigecycline. Sedangkan, K. pneumoniae penghasil ESBL menunjukkan sensitivitas > 80% terhadap Amikacin, Ertapenem, Meropenem, dan Tigecycline. Penelitian ini menyoroti tingginya prevalensi E.coli dan K.pneumoniae penghasil ESBL di rumah sakit rujukan tersier di Bali. Analisis yang seksama dari antibiogram kedua spesies penghasil ESBL tersebut akan membantu menyusun kebijakan penggunaan antibiotik dan pencegahan, pengendalian penyebaran bakteri penghasil ESBL.Kata Kunci: Escherichia coli; Klebsiella pneumoniae; Extended Spectrum Beta Lactamases; ICU dan Non-ICU

scholarly journals Pathogens distribution and antimicrobial resistance in bloodstream infections in twenty-five neonatal intensive care units in China, 2017–2019

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jing Liu ◽  
Zengyu Fang ◽  
Yonghui Yu ◽  
Yanjie Ding ◽  
Zhijie Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intensive Care ◽  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Neonatal Intensive Care ◽  
Late Onset ◽  
Bloodstream Infections ◽  
Neonatal Intensive Care Units ◽  
Late Onset Sepsis ◽  
Resistance Rates

Abstract Background Overcrowding, abuse of antibiotics and increasing antimicrobial resistance negatively affect neonatal survival rates in developing countries. We aimed to define pathogens and their antimicrobial resistance (AMR) of early-onset sepsis (EOS), hospital-acquired late-onset sepsis (HALOS) and community-acquired late-onset sepsis (CALOS) in 25 neonatal intensive care units (NICUs) in China. Study design This retrospective descriptive study included pathogens and their AMR from all neonates with bloodstream infections (BSIs) admitted to 25 tertiary hospitals in China from January 1, 2017, and December 31, 2019. We defined EOS as the occurrence of BSI at or before 72 h of life and late-onset sepsis (LOS) if BSI occurred after 72 h of life. LOS were classified as CALOS if occurrence of BSI was ≤ 48 h after admission, and HALOS, if occurrence was > 48 h after admission. Results We identified 1092 pathogens of BSIs in 1088 infants from 25 NICUs. Thirty-two percent of all pathogens were responsible for EOS, 64.3% HALOS, and 3.7% CALOS. Gram-negative (GN) bacteria accounted for a majority of pathogens in EOS (56.7%) and HALOS (62.2%). The most frequent pathogens causing EOS were Escherichia coli (27.2%) and group B streptococcus (GBS; 14.6%) whereas in CALOS they were GBS (46.3%) and Staphylococcus aureus (41.5%). Klebsiella pneumoniae (27.9%), Escherichia coli (15.7%) and Fungi (12.8%) were the top three isolates in HALOS. Third-generation cephalosporin resistance rates in GN bacteria ranged from 9.7 to 55.6% in EOS and 26% to 63.3% in HALOS. Carbapenem resistance rates in GN bacteria ranged from 2.7 to 31.3% in HALOS and only six isolates in EOS were carbapenem resistant. High rates of multidrug resistance were observed in Klebsiella pneumoniae (60.7%) in HALOS and in Escherichia coli (44.4%) in EOS. All gram-positive bacteria were susceptible to vancomycin except for three Enterococcus faecalis in HALOS. All-cause mortality was higher among neonates with EOS than HALOS (7.4% VS 4.4%, [OR] 0.577, 95% CI 0.337–0.989; P = 0.045). Conclusions Escherichia coli, Klebsiella pneumoniae and GBS were the leading pathogens in EOS, HALOS and CALOS, respectively. The high proportion of pathogens and high degree of antimicrobial resistance in HALOS underscore understanding of the pathogenesis and emphasise the need to devise effective interventions in developing countries.

scholarly journals Gastrointestinal Carriage Is a Major Reservoir of Klebsiella pneumoniae Infection in Intensive Care Patients

2017 ◽  
Vol 65 (2) ◽  
pp. 208-215 ◽  
Author(s):  
Claire L. Gorrie ◽  
Mirjana Mirčeta ◽  
Ryan R. Wick ◽  
David J. Edwards ◽  
Nicholas R. Thomson ◽  
...  
Keyword(s):  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Intensive Care Patients
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