THE ANTIBACTERIAL ACTIVITY OF NIGELLA SATIVA AGAINST MULTI-DRUG RESISTANT PSEUDOMONAS AERUGINOSA ISOLATED FROM DIABETIC WOUND INFECTIONS

2017 ◽  
Vol 2 (1) ◽  
pp. 113-134
Author(s):  
Wagih A. El-Shouny ◽  
◽  
Sameh S. Ali ◽  
Shymaa F. Fayed ◽  
◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Nigella Sativa ◽  
Drug Resistant ◽  
Wound Infections ◽  
Diabetic Wound

scholarly journals In vitro Antibacterial Activity of Organic Extracts of Aloe barbadensis against Multi-drug resistant Pseudomonas aeruginosa Isolated from Wound Specimens

2018 ◽  
Vol 5 ◽  
pp. 69-76
Author(s):  
Mamata Adhikari ◽  
Anil Kumar Sah ◽  
Dev Raj Joshi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Inhibitory Action ◽  
Therapeutic Option ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Aloe Barbadensis ◽  
Organic Extracts ◽  
In Vitro Antibacterial Activity

Objectives: In order to investigate alternate therapeutic option, this study was carried out to assess the in vitro antibacterial activity of gel extract of Aloe barbadensis against multiple antibiotic resistant Pseudomonas aeruginosa isolated from wound specimens. Methods: A total of 180 different wound specimens collected in a hospital, were subjected to isolate and identify P. aeruginosa by cultural methods. Antibiotic susceptibility testing was done by Kirby- Bauer disc diffusion method to screen multidrug resistant isolates. A. barbadensis extracts were prepared using aqueous and organic solvents and their in vitro inhibitory action was evaluated by agar well diffusion methods. Results: Out of total, 38 (21.1%) of the wound specimens showed the occurrence of P. aeruginosa, among which 15 (39%) isolates were multi-drug resistant. Organic extracts of various concentrations (0.2 - 0.8 v/v %) inhibited 66.7% of MDR and all non-MDR (n = 23) P. aeruginosa with zone of inhibition ranging from 9.5 ±1.0 to 21.3 ± 2.2 mm but not by aqueous extract. A positive Pearson’s correlation (r=0.983) was found between antibacterial effect and concentrations of the extracts. The antibacterial activity of organic extracts was statistically associated with antibiotic resistance profile of the organism (p<0.05). Conclusion: Organic extracts of A. barbadensis revealed variable in vitro inhibitory action against both MDR and non-MDR P. aeruginosa isolated from wound specimens. Although further confirmation is needed, aloe gel extract may be applied as an alternate treatment option.

scholarly journals Characterisation of the Prevailing Multidrug Pseudomonas aeruginosa Strains from Surgical Wound Using 16S rRNA Sequencing Technique

2021 ◽  
Vol 28 (4) ◽  
pp. 37-49
Author(s):  
Eremwanarue Osagie Aibuedefe ◽  
Nwawuba Stanley Udogadi ◽  
Shittu Olalekan Hakeem
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Wound Infections ◽  
Surgical Wound ◽  
Specific Primer ◽  
Pseudomonas Spp ◽  
Multiple Drug Resistant ◽  
Rrna Sequencing

Background: Pseudomonas aeruginosa (P. aeruginosa) is prevalent in hospital-acquired surgical wound infections. It exhibits both innate and acquired resistance to a broad range of antimicrobials and remains a principal problem in clinical practice. Methods: In total, 284 sterile surgical wound swabs (142 each) were collected from two government hospitals: Central Hospital Benin (CHB) and University of Benin Teaching Hospital (UBTH) in Benin City, Nigeria. Pseudomonas spp. isolated from both hospitals were screened with eight different antibiotics by way of disk diffusion method. Polymerase chain reaction (PCR) amplification of 34 multiple drug-resistant isolates was carried out using genus-specific primer set on extracted genomic DNA for the identification of Pseudomonas spp. and substituent 16S rRNA sequencing to determine the prevailing strains in the two locations. Results: Sixty-two Pseudomonas spp. were isolated from the two locations (27 isolates from CHB and 35 isolates from the UBTH). Surgical wound infections screened with regularly used antibiotics revealed that 17 (62.9%) isolates from CHB and 20 (57.1%) isolates from UBTH were multiple drug resistant Pseudomonas spp. PCR identification using Pseudomonas spp. specific primer showed that 16 (94.1%) isolates from CHB and 18 (90%) isolates from UBTH were confirmed. 16S DNA sequencing revealed that P. aeruginosa strain H25883 was dominant in both locations. Conclusion: High antibiotic resistance among P. aeruginosa isolates was established in our study. PCR technique revealed a more reliable method of bacterial identification. H25883 strain of P. aeruginosa is the prevalent strain in both locations and it should be given attention in nosocomial surgical wound infections.

scholarly journals Characterization of the antibacterial activity of Bald’s eyesalve against drug resistant Staphylococcus aureus and Pseudomonas aeruginosa

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0208108 ◽  
Author(s):  
Amanda L. Fuchs ◽  
Alan J. Weaver ◽  
Brian P. Tripet ◽  
Mary Cloud B. Ammons ◽  
Martin Teintze ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Drug Resistant

scholarly journals Synthesis of silver nanoparticles using Nigella sativa seed extracts and assessment of their antibacterial activity

2020 ◽  
Vol 11 (2) ◽  
pp. 2525-2532
Author(s):  
Sheik Shehensha ◽  
Jyothi M V
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Nigella Sativa ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Resistant Strains ◽  
Nigella Sativa Seed ◽  
Seed Extracts

Silver nanoparticles were biosynthesized from Nigella sativa seed extracts using ethanol and chloroform. The antibacterial activity of silver nanoparticles against some drug-resistant bacteria has been established, but further study is needed to assess whether these particles could be an option for the treatment and prevention of drug-resistant microbial infections. Synthesized nanoparticles were characterized and screened for their antibacterial properties on resistant strains. The biosynthesized silver nanoparticles were characterized by UV-Visible, FTIR, Dynamic light scattering and Scanning Electron Microscope (SEM) analysis. The antibacterial action of biosynthesized silver nanoparticles was assessed by Microtitre Broth dilution process using Ciprofloxacin as standard, against resistant strains like Pseudomonas aeruginosa, Clostridium difficile, Klebsiella pneumoniae and Streptococcus pyogenes. The Silver nanoparticles obtained from chloroform extract of Nigella sativa seeds were more effective against Pseudomonas aeuruginosa, Clostridium difficile and Streptococcus pyogenes; than ethanolic seed extracts at 120 µL. Our data propose that the silver nanoparticles are effective against a variety of drug-resistant bacteria, which makes them a potential candidate for use in pharmaceutical products that may help to treat drug-resistant pathogens in different clinical environments. The present study focuses on the ability of phytoconstituents capped with silver nitrate can be used to treat infections caused by resistant bacteria

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