scholarly journals MORPHOLOGICAL CHANGES OF PERIPHERAL BLOOD CELLS AT DIFFERENT OPTIONS OF THE TREATMENT OF THE OPISTHORCHIASIS IN THE EXPERIMENT

2018 ◽  
Vol 26 (2) ◽  
pp. 17-21
Author(s):  
A. A. Sidelnikova
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Morphological Study ◽  
Morphological Changes ◽  
Blood Platelets ◽  
Treatment Options ◽  
Peripheral Blood Cells ◽  
Experimental Animals ◽  
And Control ◽  
All Treatment

A morphological study of the formed elements of peripheral blood in experimental animals (rabbits) induced by opisthorchiasis in the treatment Biltricid, Acorsol and Triad Premium. When applying Acorsol and Triad Premium discovered toxic granularity of the cytoplasm of segmented and band at pseudoeosinophils. Segmented shape was characterized by hypersegmented kernel. When applying Biltricid stab pseudoeosinophils in the blood is not detected. The segmented pseudoeosinophils had a pronounced toxic or grit, or completely had no granules in the cytoplasm were single or multiple vacuoles calf Knyazkova is the Case. Young forms are discovered in the application of the Triad Premium. Eosinophils in all cases had partial basophilic granules. In the cytoplasm of basophils in all groups of granules are partially absent, but to a greater extent in the treatment of Acorsol. Form Turk in contrast to the control were isolated. Forms Reader in a smaller number of detected only with the use of Acorsol. The shadows Botkin-Gumprecht-Klein considerable amount detected in the application of Biltricid and in control. In the study of red blood cells poikilocytosis detected in all cases. But when you apply Biltricid still identified anisochromia, basophilic punctuation. Thus, the most pronounced changes of erythrocytes and leucocytes was observed after treatment of opisthorchiasis Biltricid, with other drugs and control. But unlike Biltricid, in the treatment of opisthorchiasis Triad Premium and Acorsol visually, there was an increase in the size of blood platelets. When morphometry diameter pseudoeosinophils and eosinophils in animals with the treatment and the control was normal according to the literature. However, without treatment, the diameter of pseudoeosinophils and eosinophils was greater than in cells of animals with all treatment options. There were no significant differences in the diameter of eosinophils in the treatment with Triad Premium and Biltricide.

Epigenetic regulation of SNCA gene expression in Parkinson’s disease

2020 ◽  
pp. 96-98
Author(s):  
А.К. Емельянов ◽  
А.О. Лавринова ◽  
Н.В. Мельникова ◽  
А.А. Дмитриев ◽  
И.В. Милюхина ◽  
...  
Keyword(s):  
Gene Expression ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Cpg Island ◽  
Alpha Synuclein ◽  
Peripheral Blood Cells ◽  
Snca Gene ◽  
Intron 1 ◽  
And Control ◽  
First Time

В настоящем исследовании проведена оценка уровня мРНК и белка генов SNCA, DNMT1, а также степени метилирования интрона 1 гена SNCA в CD45+ клетках периферической крови пациентов со спорадической болезнью Паркинсона (БП) и индивидуумов контрольной группы. Впервые было выявлено снижение концентрации белка DNMT1 в CD45+ клетках периферической крови пациентов с БП по сравнению с группой контроля. Обнаружено увеличение уровня мРНК гена DNMT1 у пациентов с БП по сравнению с контролем. Не выявлено статистически значимых различий при сравнении степени метилирования интрона 1 гена SNCA в CD45+ клетках периферической крови пациентов с БП и контроля. В группе контроля выявлена обратная корреляция степени метилирования отдельных CpG островков с концентрацией белка альфа-синуклеина и уровнем мРНК гена SNCA. Проведенное исследование позволяет предположить участие гена DNMT1 в патогенезе БП и отсутствие ассоциации степени метилирования интрона 1 гена SNCA с БП. The aim of this study was to assess the level of mRNA and protein of the SNCA, DNMT1 genes, as well as intron 1 methylation of the SNCA gene in CD45 + peripheral blood cells of patients with sporadic PD and control individuals. For the first time, a decrease in the concentration of DNMT1 protein in CD45 + peripheral blood cells from PD patients compare to controls was revealed. An increase in DNMT1 gene expression in PD patients compare to controls was found. No differences in intron 1 methylation of the SNCA gene in CD45 + peripheral blood cells was found between PD patients and controls. An inverse correlations between methylation level of 21, 22 CpG island in intron1 of SNCA gene and mRNA SNCA gene and alpha-synuclein protein level were found. The study suggests the involvement of DNMT1 in the pathogenesis of PD and the lack of association of PD with intron 1 methylation of the SNCA gene.

Morphological changes in peripheral blood cells and skin in amiodarone-treated patients

1986 ◽  
Vol 114 (2) ◽  
pp. 189-196 ◽  
Author(s):  
K. RAPPERSBERGER ◽  
K. KONRAD ◽  
E. WIESER ◽  
H. WEBER ◽  
K. WOLFF
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Morphological Changes ◽  
Peripheral Blood Cells

Automatic wedge smears preparation may cause traumatic morphological changes in peripheral blood cells

2017 ◽  
Vol 71 (2) ◽  
pp. 168-171 ◽  
Author(s):  
Antonio La Gioia ◽  
Maurizio Fumi ◽  
Paola Pezzati ◽  
Fiamma Balboni ◽  
Ylenia Pancione ◽  
...  
Keyword(s):  
Physical Characteristic ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Morphological Changes ◽  
Peripheral Blood Cells ◽  
Traumatic Injuries ◽  
Morphological Evaluation ◽  
Clinical Laboratories ◽  
Blood Smears ◽  
Very High

In recent years, several automated analysers that prepare and stain blood smears have been introduced in clinical laboratories. Despite the use of instrumental settings based on physical characteristic of individual samples, traumatic injuries of neutrophil and lymphocytes can be observed. Some samples present a very high percentage of damaged cells, allowing the speculation that a cellular susceptibility may enhance mechanical traumatism. These artefacts can puzzle morphological evaluation in both traditional and digitised microscopy; in addition, unskilled operators can be misled.

Receptor Mediated Binding of the Fibrinolytic Components, Plasminogen and Urokinase, to Peripheral Blood Cells

1987 ◽  
Vol 58 (03) ◽  
pp. 936-942 ◽  
Author(s):  
Lindsey A Miles ◽  
Edward F Plow
Keyword(s):  
T Cells ◽  
B Cell ◽  
Peripheral Blood ◽  
Binding Sites ◽  
Blood Cells ◽  
High Capacity ◽  
Red Cells ◽  
Peripheral Blood Cells ◽  
Cellular Functions ◽  
Fibrinolytic Proteins

SummaryGlu-plasminogen binds to platelets; the monocytoid line, U937, and the human fetal fibroblast line, GM1380 bind both plasminogen and its activator, urokinase. This study assesses the interaction of these fibrinolytic proteins with circulating human blood cells. Plasminogen bound minimally to red cells but bound saturably and reversibly to monocytes, granulocytes and lymphocytes with apparent Kd values of 0.9-1.4 μM. The interactions were of high capacity with 1.6 to 49 × 105 sites/cell and involved the lysine binding sites of plasminogen. Both T cells and non-rosetting lymphocytes and two B cell lines saturably bound plasminogen. Urokinase bound saturably to gianulocytes, monocytes, non-rosetting lymphocytes and a B cell line, but minimally to T cells, platelets and red cells. Therefore, plasminogen binding sites of high capacity, of similar affinities, and with common recognition specificities are expressed by many peripheral blood cells. Urokinase receptors are also widely distributed, but less so than plasminogen binding sites. The binding ol plasminogen and/ or urokinase to these cells may lead to generation of cell- associated proteolytic activity which contributes to a variety of cellular functions.

scholarly journals RNA Sequencing of Human Peripheral Blood Cells Indicates Upregulation of Immune-Related Genes in Huntington's Disease

2020 ◽  
Vol 11 ◽  
Author(s):  
Miguel A. Andrade-Navarro ◽  
Katja Mühlenberg ◽  
Eike J. Spruth ◽  
Nancy Mah ◽  
Adrián González-López ◽  
...  
Keyword(s):  
Gene Expression ◽  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Trinucleotide Repeat ◽  
Neurodegenerative Disorder ◽  
Gene Expression Signature ◽  
Interferon Response ◽  
Peripheral Blood Cells

Huntington's disease (HD) is an autosomal dominantly inherited neurodegenerative disorder caused by a trinucleotide repeat expansion in the Huntingtin gene. As disease-modifying therapies for HD are being developed, peripheral blood cells may be used to indicate disease progression and to monitor treatment response. In order to investigate whether gene expression changes can be found in the blood of individuals with HD that distinguish them from healthy controls, we performed transcriptome analysis by next-generation sequencing (RNA-seq). We detected a gene expression signature consistent with dysregulation of immune-related functions and inflammatory response in peripheral blood from HD cases vs. controls, including induction of the interferon response genes, IFITM3, IFI6 and IRF7. Our results suggest that it is possible to detect gene expression changes in blood samples from individuals with HD, which may reflect the immune pathology associated with the disease.

Transplantation of Bone Marrow as Compared with Peripheral-Blood Cells from HLA-Identical Relatives in Patients with Hematologic Cancers

2001 ◽  
Vol 344 (3) ◽  
pp. 175-181 ◽  
Author(s):  
William I. Bensinger ◽  
Paul J. Martin ◽  
Barry Storer ◽  
Reginald Clift ◽  
Steven J. Forman ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells
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