ANTI-DENGUE TYPE 2 VIRUS ACTIVITIES OF ZINC (II) COMPLEX COMPOUNDS WITH 2-(2,4 -DIHYDROXYPHENYL)-3,5,7-TRIHYDROXYCROMEN-4-ONE LIGANDS IN VERO CELLS

Dengue virus (DENV) is a disease that is transmitted through Aedes aegypti and Aedes albopictus mosquitoes, and is spread in tropical and sub-tropical regions. Now, dengue or antiviral vaccines for humans do not yet exist, but there are great efforts to achieve this goal. Complex compounds are reported to fungicidal, bactericidal and antiviral activity. Antiviral activity against DENV is an important alternative to the characterization and development of drugs candidate. The purpose of this study was to study zinc(II) compounds with 2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxycromen-4-one ligand on DENV-2 replication in Vero cells. Vero cell lines (African green monkey kidney) was used in this study, maintained and propagated in Minimum Essential Eagle Medium containing 10% fetal bovine serum at 37°C in 5% CO2. The activity of dengue virus was carried out by enzyme-immunosorbent assay (ELISA) method and CellTiter96® Non-Radioactive Proliferation. The value of activity inhibition (IC50) of complex compounds with variations of mol metal: ligand 1:2, 1:3, and 1:4 against dengue virus type 2 (DENV2) was 2.44 μg/ml, 2.75 μg/ml, respectively and 2.00 μg/ml, also the toxicity value (CC50) of complex compounds with variation mol metal: ligand 1:4 for Vero cells is 3.59 μg/ml. The results of this study were indicate that these properties have been shown to inhibit anti-dengue type 2 virus (DENV-2), but are also toxic in Vero cells. Including previous study about complex compound interaction with dengue virus type 2 activity, Zn(II) more reactive compound then Cu(II), and Co(II). The comparison with Cu(II) complex compound, it has been revealed that Co(II) and Zn(II) is more toxic, was found to be nontoxic to human erythrocyte cells even at a concentration of 500 μg/ml.

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In Vitro Antiviral Activity of Pinus merkusii (Pinaceae) Stem Bark and Cone against Dengue Virus Type-2 (DENV-2)

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00641 ◽

2021 ◽

pp. 3705-3708

Author(s):

Arif Nur Muhammad Ansori ◽

Amaq Fadholly ◽

Annise Proboningrat ◽

Suhailah Hayaza ◽

Raden Joko Kuncoroningrat Susilo ◽

...

Keyword(s):

Antiviral Activity ◽

Dengue Virus ◽

Virus Type ◽

Stem Bark ◽

Endemic Region ◽

In Vivo Evaluation ◽

African Green Monkey Kidney ◽

Antiviral Compound ◽

Dengue Virus Type 2

Dengue is a major mosquito-borne disease that currently has no effective antiviral or vaccine available. Recently, Indonesia is one of the largest countries in the dengue-endemic region, with a total population of more than 250 million. In the present study, the antiviral activity of P. merkusii stem bark and cone were evaluated against dengue virus type-2 (DENV-2; NCBI accession number: KT012509) isolated from Surabaya, Indonesia. We revealed that P. merkusii stem bark and cone inhibited DENV-2 in Vero cells (originally from African green monkey kidney) with IC50= 140.63 μg/mL and 73.78 μg/mL, CC50= 89.65 μg/mL and 249.5 μg/mL, SI= 0.64 and 3.38, respectively. The findings presented here suggest that P. merkusii stem bark and cone exerts potent antiviral activity against DENV-2. Hence, P. merkusii stem bark and cone are potent to inhibit DENV-2 and should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV-2.

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Eﬀect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell

Indonesian Journal of Tropical and Infectious Disease ◽

10.20473/ijtid.v8i3.11776 ◽

2020 ◽

Vol 8 (3) ◽

pp. 183

Author(s):

Teguh Hari Sucipto ◽

Aswandi Wibrianto ◽

Fahimah Martak ◽

Siti Churrotin ◽

Ilham Harlan Amarullah ◽

...

Keyword(s):

Dengue Virus ◽

Vero Cell ◽

Complex Compound ◽

Inhibitory Activity ◽

Vero Cells ◽

Inhibitory Effect ◽

Complex Compounds ◽

Antiviral Compound ◽

First Time

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many eﬀorts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitory eﬀect on the replication of DENV-2 in cell culture. DENV replication was measured by antiviral activity assay and cytotoxicity assay. The inhibitory activity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by Viral ToxGloTM Assay. The cytotoxicity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by CellTiter96® AQuoeus assay. The inhibitory concentration (IC50) of Zinc(II)-2,4,5-triphenyl- 1H-imidazole against dengue virus type-2 was 34.42 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was <100 μg/ml. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole complex and its high toxicity in Vero cells. Further studies are not required before investigated Zinc(II)-2,4,5-triphenylimidazole can be applied in the treatment of DENV-2 infections

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Antiviral activity of Faramea bahiensis leaves on dengue virus type-2 and characterization of a new antiviral flavanone glycoside

Phytochemistry Letters ◽

10.1016/j.phytol.2017.01.013 ◽

2017 ◽

Vol 19 ◽

pp. 220-225 ◽

Cited By ~ 6

Author(s):

Adriana C. Nascimento ◽

Ligia M.M. Valente ◽

Mário Gomes ◽

Rodolfo S. Barboza ◽

Thiago Wolff ◽

...

Keyword(s):

Antiviral Activity ◽

Dengue Virus ◽

Virus Type ◽

Flavanone Glycoside ◽

Dengue Virus Type 2

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Zinc accelerates dengue virus type 2-induced apoptosis in Vero cells

FEBS Letters ◽

10.1016/s0014-5793(02)02991-5 ◽

2002 ◽

Vol 524 (1-3) ◽

pp. 20-24 ◽

Cited By ~ 10

Author(s):

Norazizah Shafee ◽

Sazaly AbuBakar

Keyword(s):

Dengue Virus ◽

Virus Type ◽

Vero Cells ◽

Induced Apoptosis ◽

Dengue Virus Type 2

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In vitro Antiviral Activity of Bacopa monnieri (l.) Wettst Roots Methanolic Extract Against Dengue Virus Type 2

Acta Scientific Medical Sciences ◽

10.31080/asms.2021.05.1043 ◽

2021 ◽

Vol 5 (10) ◽

pp. 114-119

Author(s):

Keerthy M ◽

M Arya Lakshmi ◽

B Ganga Rao

Keyword(s):

Antiviral Activity ◽

Dengue Virus ◽

Virus Type ◽

Methanolic Extract ◽

Bacopa Monnieri ◽

Dengue Virus Type 2

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Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells

The Scientific World JOURNAL ◽

10.1155/2013/282734 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 36

Author(s):

Kleber Juvenal Silva Farias ◽

Paula Renata Lima Machado ◽

Benedito Antônio Lopes da Fonseca

Keyword(s):

Dengue Virus ◽

Virus Type ◽

Mammalian Cells ◽

Plaque Assay ◽

Antiviral Drug ◽

Vero Cells ◽

Significant Difference ◽

Dengue Virus Type 2

Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of Vero and C6/36 cells infected with dengue virus type 2 (DENV-2). Real-time RT-PCR and plaque assays were used to quantify the DENV-2 load in infected Vero and C6/36 cells after chloroquine treatment. Our results showed that a dose of 50 μg/ml of chloroquine was not toxic to the cells and induced a statistically significant inhibition of virus production in infected Vero cells when compared to untreated cells. In C6/36 cells, chloroquine does not induce a statistically significant difference in viral replication when compared to untreated cells, showing that this virus uses an unlikely pathway of penetration in these cells, and results were also confirmed by the plaque assay (PFU). These data suggest that the inhibition of virus infection induced by chloroquine is due to interference with acidic vesicles in mammalian cells.

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