scholarly journals RESISTANCE PATTERN OF Escherichia Coli AGAINTS ANTIBIOTICS IN URINARY TRACT INFECTION PATIENTS IN RSUD DR. SOETOMO SURABAYA

2020 ◽  
Vol 1 (2) ◽  
pp. 53
Author(s):  
Novi Ariana ◽  
Pestariati Pestariati ◽  
Retno Sasongkowati ◽  
Debby Kusumaningrum
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Clavulanic Acid ◽  
Pathogenic Bacteria ◽  
Supportive Therapy ◽  
Resistance Pattern ◽  
Amoxicillin Clavulanic Acid

Pathogenic bacteria cause Urinary Infection imore or lessi about 85 - 95% are bacteria Escherichia coli. The treatment of Urinary Tract Infection (UTI) requires adequate supportive therapy and antibiotics, but antibiotic resistance is an obstacle in UTI treatment. This study aims to determine the types of antibiotics that are resistant to Escherichia coli in patients with UTI. This is an observational study with a descriptive method to determine the type of antibiotic-resistant to Escherichia coli in patients with UTI in the Laboratory of Clinical Microbiology RSUD Dr. Soetomo. Antibiotics that were resistant to Escherichia coli in UTI patients were cefazolin (80.49%), Amoxicillin Clavulanic Acid (73.17%), Ampicillin (73.17%), Tetracycline (68.29%), while antibiotics that were resistant to Escherichia coli ESBL was Amoxicillin Clavulanic Acid (100%), Ampicillin (100%), Cefazolin (99.7%), Aztreonam (99.7%), Ceftazidime (99.7%). The prevalence of Escherichia coli in UTI patients was 27.5%, while Escherichia coli ESBL was 72.5%. Based on the results of the research that has been obtained, it is better to do research on antibiotic resistance patterns periodically.

scholarly journals ANTIBIOTIC SUSCEPTIBILITY OF ESCHERICHIA COLI ISOLATE FROM URINARY TRACT INFECTION OF THALASSEMIC PATIENTS IN THI-QAR PROVINCE

2020 ◽  
Vol 28 (28) ◽  
pp. 22-33
Author(s):  
Alaa Abood Yasir OKAB ◽  
Manal B SALIH
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Clavulanic Acid ◽  
Rural Areas ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Amoxicillin Clavulanic Acid ◽  
Tract Infections

Escherichia coli (E. coli) is the most common type of pathogen that causes Urinary tract infection disease. It can be presented as a pathogenic or non-pathogenic strain and found not only in the animal but also in the human intestine. This bacterium can cause opportunistic infection when the human host comprised of thalassemia patients or changes the healthy hemostatic flora. This study aimed to analyze the presence of bacteria in thalassemia patients with urinary tract infection. A total of 303 samples were collected during the period from August 2019 to January 2020 from thalassemia patients who suffered from urinary tract infection. The results showed that there were 6.9% of patients infected with E. coli, 2.6% of patients were infected with S. aureus, 0.7% with both Proteus and Klebsiella, while 89.1% of patients had a negative sample for bacteria. Also, the incidence of urinary tract infections in females is higher than in males. Besides, its occurrence in rural areas is higher than in city residents. Moreover, among 16 antibiotics tested to sensitize bacteria to antibiotics, Imipenem showed 100% efficacy on all isolated bacteria. In contrast, Netilmicin showed 80.1% efficacy, Gentamycin 80.1%, and Amikacin 76.2%. Ampicillin, Aztreonam, Amoxicillin-Clavulanic Acid, Tetracycline, and Ticarcillin-Clavulanic Acid, did not show any effectiveness toward the bacteria while other antibiotics showed different activities. Furthermore, the isolated microbes from thalassemia patients were the highest resistance to antibiotics in comparison with other studies, and this antibiotic-resistant may be due to the weakening of the patient's immune status and frequent blood taking and the antibodies it contains.

scholarly journals Antibiotic Resistance Pattern of Extended Spectrum Beta Lactamase Producing Escherichia coli Isolated From Patients With Urinary Tract Infection in Morocco

2021 ◽  
Vol 11 ◽  
Author(s):  
Mohamed Kettani Halabi ◽  
Fatima Azzahra Lahlou ◽  
Idrissa Diawara ◽  
Younes El Adouzi ◽  
Rabiaa Marnaoui ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples ◽  
Resistance Pattern ◽  
E Coli ◽  
Antibiotic Resistance Pattern ◽  
Extended Spectrum

Extended-spectrum β-lactamases producing Escherichia coli (ESBL-EC) lend resistance to most β-lactam antibiotics. Because of limited treatment options, ESBL-EC infections are generally more difficult to treat, leading to higher hospital costs, reduced rates of microbiological and clinical responses, and a threat to the patient’s life. This study aimed to determine the antibiotic resistance pattern of ESBL-EC isolated from patients with urinary tract infection in Morocco. This retrospective laboratory-based study was conducted at Cheikh Khalifa International University Hospital, Casablanca, from January 2016 to June 2019. A total of 670 urine samples were collected from urinary tract infection patients and processed by standard microbiological methods. In vitro susceptibility testing to different antibiotics of all identified isolates of Escherichia coli (E. coli) was performed following Kirby–Bauer’s disc diffusion method on Mueller–Hinton Agar according to the EUCAST standards. The reviewing of ESBL-EC was confirmed by the appearance of a characteristically shaped zone referred to as a “champagne cork” using the Combined Disk Test. Among a total of 438 E. coli isolated from nonrepetitive urine samples, two hundred fifty-nine (59%) were ESBL-EC, of which 200 (77%) were isolated from adult patients (over the age of 50) and the majority were female. All ESBL-EC isolates were resistant to third-generation cephalosporin and quinolones and sensitive to carbapenem and fosfomycin. Knowledge of antimicrobial resistance patterns in ESBL-EC, the major pathogen associated with urinary tract infection, is indispensable as a guide in choosing empirical antimicrobial treatment.

scholarly journals Antibiotic Susceptibility of Escherichia Coli Isolate from Urinary Tract Infection Of Thalassemic Patients in Thi-Qar Province

2020 ◽  
Vol 28 (28) ◽  
pp. 22-33
Author(s):  
Alaa Abood Yasir Okab ◽  
Manal Salih
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Clavulanic Acid ◽  
Rural Areas ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Amoxicillin Clavulanic Acid ◽  
Tract Infections

Escherichia coli (E. coli) is the most common type of pathogen that causes Urinary tract infection disease. It can be presented as pathogenic or non-pathogenic strain and found not only in the animal but also in the human intestine. This bacterium can cause opportunistic infection when the human host comprised of thalassemia patients or changes the healthy hemostatic flora. This study aimed to analyze the presence of bacteria in thalassemia patients with urinary tract infection. A total of 303 samples were collected during the period from August 2019 to January 2020 from thalassemia patients who suffered from urinary tract infection. The results showed that there were 6.9% of patients infected with E. coli, 2.6% of patients were infected with S. aureus, 0.7% with both Proteus and Klebsiella, while 89.1% of patients had a negative sample for bacteria. Also, the incidence of urinary tract infections in females is higher than in males. Besides, its occurrence in rural areas is higher than in city residents. Moreover, among 16 antibiotics tested to sensitize bacteria to antibiotics, Imipenem showed 100% efficacy on all isolated bacteria. In contrast, Netilmicin showed 80.1% efficacy, Gentamycin 80.1%, and Amikacin 76.2%. Ampicillin, Aztreonam, Amoxicillin-Clavulanic Acid, Tetracycline, and Ticarcillin-Clavulanic Acid, did not show any effectiveness toward the bacteria while other antibiotics showed different activities. Furthermore, the isolated microbes from thalassemia patients were the highest resistance to antibiotics in comparison with other studies, and this antibiotic-resistant may be due to the weakening of the patient's immune status and frequent blood taking and the antibodies it contains.

scholarly journals Antimicrobial Susceptibility Patterns of Escherichia coli among Tunisian Outpatients with Community-Acquired Urinary Tract Infection (2012-2018)

2020 ◽  
Vol 14 (4) ◽  
pp. 200-205
Author(s):  
Nawel Daoud ◽  
Manel Hamdoun ◽  
Hela Hannachi ◽  
Chedlia Gharsallah ◽  
Wiem Mallekh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Clavulanic Acid ◽  
Antimicrobial Susceptibility ◽  
Diffusion Method ◽  
E Coli ◽  
Amoxicillin Clavulanic Acid ◽  
Susceptibility Patterns

<b><i>Introduction:</i></b><b> </b>Community-acquired urinary tract infection is one of the most common reasons for consultation in everyday practice; it represents a major source of antibiotic consumption. <i>Escherichia coli</i> (<i>E. coli</i>) is the main pathogen incriminated. <b><i>Objective:· </i></b>The aim of this study was to evaluate antimicrobial susceptibility patterns of community-acquired uropathogenic <i>E coli</i> throughout a 7-year period. <b><i>Methodology:</i></b><b> </b>All strains of <i>E. coli</i> isolated from urine samples between January 1st 2012 and December 31st 2018 were included. Presence of ≥ 10<sup>3</sup> CFU/ml in urine culture media was considered as significant for urinary tract infection. The identification of <i>E. coli</i> strains was realized using standard laboratory techniques. Antibiotic susceptibility testing was performed using the disk diffusion method according to the CA-SFM/ EUCAST criteria. <b><i>Results: </i></b>A total of 1,335 <i>E. coli</i> strains were isolated. Overall susceptibility rates to antimicrobial agents were as follows: ampicillin 39.1%, amoxicillin-clavulanic acid 64.9%, cefotaxime 94.9%, trimethoprim/sulfamethox-azole 67.6%, ciprofloxacin 89.2%, ofloxacin 86.9%, amikacin 98.6%, gentamicin 93.9%, nitrofurantoin 97.6% and fosfomycin 99.3%. All isolates were susceptible to carbapenems. The frequency of extended spectrum beta-lactamases-producing <i>E. coli</i> strains was 4.7%. Susceptibility rates of <i>E. coli</i> for ampicillin, trimethoprim/sulfamethoxazole and amikacin remained relatively stable over the study period, whereas susceptibility to amoxicillin-clavulanic acid, cefotaxime and fluoroquinolones showed a 2-phase pattern. As for gentamicin, a continuous decrease in susceptibility rates was observed. <b><i>Conclusion:</i></b><b> </b>Antimicrobial susceptibility profiles of uropathogenic <i>E. coli</i> are constantly changing, due to modifications in the antibiogram interpretation criteria and antibiotic prescription habits. Rigorous surveillance of resistance rate is necessary to determine appropriate empirical treatment and limit the spread of multiresistant strains.

scholarly journals Comparative Antibiogram of Escherichia Coli Isolated from the Urinary Tract Infection in Patients from Tertiary Care Hospital

2021 ◽  
pp. 123-128
Author(s):  
Nandkishor Bankar ◽  
Dhruba Hari Chandi ◽  
Praful Patil ◽  
Gaurav Mahajan
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Tertiary Care ◽  
Resistance Pattern ◽  
Care Hospital ◽  
Biochemical Tests ◽  
E Coli

Introduction: UTI occurs when bacteria that live inside intestine find their way into the urinary tract through the urethra. Women are familiar to be a lot of at risk of UTI than men, with a five hundredth probability of a UTI in their life. Aim: The aim of this study was to find the antibiotic resistance pattern in Escherichia coli isolated from urinary tract infection with commonly found antibiotics among different age and sex groups. Materials and Methods: All positive urine samples for Escherichia coli obtained from patients were included. On the basis of colony morphology and biochemical tests Isolates were identified. The antibiogram profile of the isolates was determined by commonly used antibiotics. Result: In this studied, total 250 patients with UTI of both sexes were included and divided into age group. Samples processed only 250 samples with the growth of E. coli were included in this studied. The isolated E.coli showed more resistant to some degree to all the antibiotics tested.  The greatest resistance was shown against Amoxycillin (15.2% isolated were sensitive) and the least resistance was shown against Imipenem (96.4% isolated were sensitive). Conclusion: The pattern of antibiotic resistance varies from place to place, region to region, and country to country. Antimicrobial resistance should be monitored on a regular basis in both the community and hospital settings.

scholarly journals Prevalence and antibiotic resistance pattern of extended spectrum beta lactamase producing Escherichia coli isolated from urinary tract infection

2018 ◽  
Vol 8 (2) ◽  
pp. 78-81 ◽  
Author(s):  
Mohsen Rajabnia ◽  
Mohammad Saad Forghani ◽  
Sabah Hasani ◽  
Mohammad Bahadoram ◽  
Mahsa Mohammadi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Resistance Pattern ◽  
Beta Lactamase ◽  
Esbl Producer ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli

Introduction: Urinary tract infection (UTI) due to extended spectrum beta-lactamase (ESBL)-producing bacteria including Escherichia coli has become widespread. Studies have shown a trend toward higher mortality, longer hospitalization, greater hospital expenses and reduced rates of clinical and microbiologic response in ESBL UTI. Objectives: The aim of this study is to determinate the prevalence and antibiotic resistance pattern of ESBL producing E. coli isolated from UTI. Patients and Methods: This cross-sectional study was conducted on 3126 samples. Urine specimens were cultured on Eosin Methylene Blue (EBM) and blood agar. The disk diffusion standard method (Kirby Bauer) was used to test the susceptibility of the drug on MullerHinton agar plates and results were reviewed based on Clinical and Laboratory Standards Institute (CLSI) criteria. The reviewing of ESBL-producing uropathogens was carried out using Combined Disk Test (CDT) by using cefotaxime (CTX; 30 µg) and cefotaximeclavulanic acid (CTX; 30 µg /CA:10 µg) disks and CLSI protocol. Results: Out of 291 E. coli isolates, 108 (37.11%) are ESBL-producer and 183 (62.89%) are non–ESBL-producer. Among ESBL-producing E. coli, the highest antibiotic resistance was observed with cefotaxime (100%), amoxicillin (97.22%) and piperacillin (96.3%) and the highest antibiotic sensitivity was observed with meropenem (93.5%), nitrofurantoin (81.48%) and gentamicin (55.56%). Conclusion: We recommended that cephalosporins, penicillins and cotrimoxazole are not suggested in the treatment of ESBL-producing E. coli. On the other hand, carbapenems as a first line and aminoglycosides as the next step in the treatment of ESBL-producing E. coli are recommended.

scholarly journals Antibiotic Resistance in Children with Recurrent or Complicated Urinary Tract Infection

2009 ◽  
Vol 48 (173) ◽  
Author(s):  
Nidal S Younish ◽  
K Qual ◽  
T Al-Awaisheh ◽  
F Al-Awaisheh ◽  
D Al-Kayed
Keyword(s):  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Recurrent Urinary Tract Infection ◽  
Complicated Urinary Tract Infection ◽  
Resistance Pattern ◽  
Candida Spp ◽  
Recurrent Uti ◽  
Antibiotics Susceptibility

INTRODUCTION:Urinary tract infection is certainly one of the most common childhood infections. Emerging resistance to the antibiotics is not unusual. Current hospitalization for children with urinary tract infection is reserved for severe or complicated cases. The aim of the present study was to determine the antibiotic resistance pattern among children with recurrent or complicated urinary tract infection.METHODS:A retrospective study carried out at Prince Hashem hospital, Zarqa city, eastern Jordan and involved 336 episodes of culture proved urinary tract infection obtained from 121 patients with recurrent UTI, who used prophylactic antibiotics during the period from April 1, 2004 to December 31, 2006. The isolated microorganisms and there antibiotics susceptibility were studied.RESULTS:Seventy three patients (60.3%) were found to have some forms of urinary tract anomaly, significantly more prevalent among male children P<0.001. Vesicoureteral reflux being the most common (58.9%). Renal scars were significantly more prevalent among those with complicated rather than recurrent urinary tract infection (64.3% vs. 16.6%, P<0.001). Gram negative organisms were the most frequent isolates in patients with recurrent and complicated urinary tract infection. Proteus, Pseudomonas and Candida spp. were more prevalent in patients with complicated (P<0.001), and isolates in patients with UTA were significantly more resistant to most antibiotics tested.CONCLUSIONS:Pediatric urine culture isolates are becoming increasingly resistant to commonly used antibiotics. Empirical treatment with Trimethoprim-Sulfamethoxazole (TMP-SMX) or Cephalexin as the initial drug is ineffective. Nitrofurantoin and Nalidixic acid can be considered as the first line antibiotics for prophylaxis and or treatment of patients with recurrent UTI, while Meropenam and Ciprofloxacin can be used empirically in treating patients with complicated UTI.Key words: Antibiotic resistance, Complicated, Recurrent, Urinary tract infection

scholarly journals Etiological and Resistance Profile of Bacteria Involved in Urinary Tract Infections in Young Children

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Antonio Sorlózano-Puerto ◽  
José María Gómez-Luque ◽  
Juan de Dios Luna-del-Castillo ◽  
José María Navarro-Marí ◽  
José Gutiérrez-Fernández
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Clavulanic Acid ◽  
Total Activity ◽  
Geographic Area ◽  
E Coli ◽  
Amoxicillin Clavulanic Acid ◽  
Tract Infections ◽  
Reduced Activity

Background.The objective of this study was to identify the bacteria most frequently responsible for urinary tract infection (UTI) in the population of under-2-year-olds in our geographic area and to evaluate the activity of antibiotics widely used for UTI treatment during a 4-year study period.Materials and Methods.A retrospective analysis was conducted of data on the identification and susceptibility of microorganisms isolated in urine samples from children under 2 years of age.Results.A total of 1,045 uropathogens were isolated.Escherichia coliaccounted for the majority (60.3%) of these, followed byEnterococcus faecalis(22.4%) andKlebsiellaspp. (6.5%). The highestE. colisusceptibility rates (>90%) were to piperacillin-tazobactam, cefuroxime, cefotaxime, ceftazidime, imipenem, gentamicin, nitrofurantoin, and fosfomycin, and the lowest were to amoxicillin-clavulanic acid and cotrimoxazole. Among all bacteria isolated, we highlight the overall high activity of piperacillin-tazobactam, imipenem, nitrofurantoin, and fosfomycin against both community and hospital isolates and the reduced activity of amoxicillin-clavulanic acid, cephalosporins, gentamicin, and cotrimoxazole. There was no significant change in the total activity of any of the studied antibiotics over the 4-year study period.Conclusion.Empiric treatment with amoxicillin-clavulanic acid, cotrimoxazole, cephalosporins, and gentamicin may be inadequate due to their limited activity against uropathogens in our setting.

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