scholarly journals The Analysis of Morphological and Genetic Characteristics of Yellowstripe Scad from Muara Baru Modern Fish Market in North Jakarta

2020 ◽  
Vol 12 (2) ◽  
pp. 308
Author(s):  
Rahmat Sawalman ◽  
Hawis Madduppa
Keyword(s):  
Dna Barcoding ◽  
Molecular Analysis ◽  
Fish Species ◽  
Morphological Analysis ◽  
Coi Gene ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Fish Market ◽  
Commercially Important ◽  
Mitochondrial Cytochrome Oxidase

HighlightIdentification and morphological analysis of Selaroides leptolepis fish.Molecular analysis using the DNA Barcoding method with the COI gene (mitochondrial cytochrome oxidase subunit I).The number of base pairs in S. leptolepis from the sequencing results was 675bp.The morphological and molecular similarities of Selaroides leptolepis fishAbstractYellowstripe scad is one of the commercially important fish from the Carangidae family, which is marketed at Muara Baru Modern Fish Market, North Jakarta. In- formation regarding the presence of Selaroides leptolepis fish in the waters allows for effective conservation, and management of marine resources. A morphological identification of fish species is still considered inaccurate, so the molecular anal- ysis is necessary. This study aims to identify commercially important fish species deriving from Muara Baru Modern Fish Market, North Jakarta, employing mor- phological, and molecular analysis. A total of 30 specimens were collected from the field. The morphological analysis utilized a visual identification method, and morphometric measurement, while molecular analysis with DNA barcoding em- ployed the mitochondrial cytochrome oxidase subunit I (COI) gene. The results of the morphological analysis indicated that the fish species were yellowstripe scad (S. leptolepis). It also followed the results of molecular analysis of DNA barcoding that the fish was a S. leptolepis species. Therefore, the combination of morphological and genetic analysis has succeeded in identifying the fish species of S. leptolepis.

Redefinition of Cornexcisia Fan & Kano, 2000 (Diptera: Sarcophagidae), with the first description of the female of Sarcophaga kurahashii (Shinonaga & Tumrasvin, 1979)

Zootaxa ◽  
2019 ◽  
Vol 4668 (3) ◽  
pp. 410-420
Author(s):  
CHAO WANG ◽  
YUNYUN GAO ◽  
THOMAS PAPE ◽  
DONG ZHANG
Keyword(s):  
Cytochrome Oxidase ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Gene Sequences ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Senior Synonym ◽  
First Time ◽  
Mitochondrial Cytochrome Oxidase

Sarcophaga Meigen, 1826 is proposed as a senior synonym of Cornexcisia Fan & Kano, 2000, syn. nov. and Fanzideia Xue, Verves & Du, 2011, syn. nov. Cornexcisia Fan & Kano, 2000, stat. rev. is given status as a subgenus and is considered a senior synonym of Fanzideia Xue, Verves & Du, 2011, syn. nov. at the subgeneric level. Cornexcisia is argued to contain S. (Cornexcisia) longicornuta (Fan & Kano, 2000), comb. nov., S. (C.) cygnocerca (Xue, Verves & Du, 2011), comb. nov., S. (C.) kurahashii (Shinonaga & Tumrasvin, 1979), subgen. comb. nov. (from Phallosphaera Rohdendorf) and S. (C.) suthep Pape & Bänziger, 2003, subgen. comb. nov. (from Rosellea Rohdendorf). Sarcophaga (C.) kurahashii is newly recorded from China (Yunnan), the male is redescribed and the female is described for the first time, supported by photographs, illustrations and mitochondrial cytochrome oxidase subunit I (COI) gene sequences. Species of Cornexcisia share an exceptionally long postpedicel in the female and the following apomorphic distiphallic appendages in the male: juxta ventro-proximally with an apically divided arm with cuticular pile, and lateral styli bifurcated from the base with each branch elongate, gently curved and slightly expanded apically. A key to the species of Cornexcisia is provided. 

scholarly journals Identification of Awaous guamensis and A. grammepomus based on morphology and molecular analysis

2017 ◽  
Vol 26 (1) ◽  
pp. 83-90
Author(s):  
Hawa Jahan ◽  
Mehnus Tabassum ◽  
Gulshan Ara Latifa
Keyword(s):  
Sequence Data ◽  
Coi Gene ◽  
Universal Primers ◽  
Morphological Identification ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Specific Variation ◽  
Near Future ◽  
Mitochondrial Cytochrome Oxidase ◽  
Awaous Guamensis

Work on two freshwater Gobi fish species such as Awaous guamensis and A. grammepomus was carried out on morphometric and meristic study for their morphological identification. Sequence data of mitochondrial cytochrome oxidase subunit I (COI) gene was used for this purpose. Around 620 bp sequences were obtained when universal primers were used. An alignment was performed between the sequences which showed some inter-specific variation. An entropy plot was generated for all the aligned positions which showed that the entropy rarely touches a scale of one, which is a sign of better alignment in the region. This work can be utilized for species identification, phylogenetic analysis, to study molecular diversity and evolution, and most importantly for the conservation of these fauna in near future. Dhaka Univ. J. Biol. Sci. 26(1): 83-90, 2017 (January)

Pamphilius japonicus feeds on Cornus: the first Old World record of Pamphiliidae (Hymenoptera) associated with Cornaceae

Zootaxa ◽  
2019 ◽  
Vol 4585 (2) ◽  
pp. 343
Author(s):  
AKIHIKO SHINOHARA ◽  
SHIN-ICHI IBUKI ◽  
TSUNEO KAKUDA ◽  
YUICHI KAMEDA
Keyword(s):  
Host Plant ◽  
First Record ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
World Record ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Old World ◽  
Cytochrome Oxidase Subunit 1 ◽  
Mitochondrial Cytochrome Oxidase

We identified newly discovered pamphiliid larvae feeding on Cornus in Tochigi and Ibaraki Prefectures, Honshu, Japan, with Pamphilius japonicus Shinohara, 1985, by molecular methods using mitochondrial cytochrome oxidase subunit 1 (COI) gene sequences. The host plant of P. japonicus was unknown. This is the first record of the Cornaceae as a host plant of the Pamphiliidae in the Old World. The larvae were solitary leaf-rollers on Cornus and discovered mainly on small young trees in shadowy forests. 

scholarly journals Identifikasi Caesio cuning berdasarkan Karakterisasi Morfometrik dan DNA Barcoding yang didaratkan di Pasar Ikan Muara Baru, Jakarta

2020 ◽  
Vol 23 (2) ◽  
pp. 199-206
Author(s):  
Muhammad Fahmi Zuhdi ◽  
Hawis Madduppa
Keyword(s):  
Dna Barcoding ◽  
Morphological Analysis ◽  
Morphological Character ◽  
Coi Gene ◽  
Fish Sample ◽  
Morphometric Characters ◽  
Precise Information ◽  
Fish Market ◽  
Pcr Method ◽  
Species Authentication

Yellow-tailed fish (Caesio cuning) have morphologically similarities with Lutjanidae families, it causes ambiguity on species authentication process. The process of species identification using morphological characteristic does not provide a precise information related to the species.This study was aimed to identify the morphometric and molecular of Yellow-tailed fish (Caesio cuning) which landed on Muara Baru Fish Market, Jakarta using COI gene. A total 30 fishes were observed their nineteen morphometric characters, and 1 fish sample was taken from the fins for DNA extraction, amplification using PCR method, electrphoresis visualization, and sequensing. Sample was analyzed by MEGA 6 software. Based on morphological analysis showed that sampel are Yellow-tailed fish which part of Caesio genus and Caesionidae family. While, genetic analysis using COI gene showed has similarities with database of Genbank NCBI. It can be concluded that identification using morphological character and DNA barcoding methode showed the species belong to Yellow-tailed fish (Caesio cunning).  Ikan Ekor Kuning (Caesio cuning) memiliki kemiripan morfologi dengan anggota famili Lutjanidae lainnya, hal tersebut menyebabkan kesulitan dalam proses autentikasinya.  Proses identifikasi pada suatu spesies menggunakan karakteristik morfologinya belum mampu memberikan informasi yang akurat terkait spesies tersebut. Penelitian ini bertujuan untuk mengidentifikasi ikan ekor kuning (Caesio cuning) yang didaratkan di Pasar Muara Baru, Jakarta melalui kajian karakteristikmorfometrik dan DNA barcoding menggunakan marka gen COI. Total 30 ikan diamati karakter morfometriknya, dan 1 sampel ikan diambil bagian siripnya untuk dilakukan ekstraksi DNA, amplfikasi PCR, elektroforesis, sekuensing dan dianalisis menggunakan aplikasi MEGA 6. Hasil analisis morfologi menunjukkan sampel ikan berasal dari genus Caesio dan termasuk famili Caesionidae. Sedangkan berdasarkan analisis secara molekuler menggunakan marka gen COI, didapatkan hasil bahwa spesies yang diamati (Caesio cuning) memiliki kemiripan dengan database GenBank NCBI. Dapat simpulkan bahwa identifikasi secara morfologi dan DNA menunjukkan bahwa spesies yang di peroleh yaitu Ikan ekor kuning (Caesio cuning).

Molecular epidemiology ofSchistosoma mansoniin Uganda: DNA barcoding reveals substantial genetic diversity within Lake Albert and Lake Victoria populations

Parasitology ◽  
2009 ◽  
Vol 136 (13) ◽  
pp. 1813-1824 ◽  
Author(s):  
J. R. STOTHARD ◽  
B. L. WEBSTER ◽  
T. WEBER ◽  
S. NYAKAANA ◽  
J. P. WEBSTER ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Nucleotide Diversity ◽  
Lake Victoria ◽  
Population Diversity ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Statistical Parsimony ◽  
Mitochondrial Cytochrome Oxidase ◽  
Representative Samples

SUMMARYRepresentative samples of UgandanSchistosoma mansonifrom Lake Albert and Lake Victoria were examined using DNA barcoding, sequence analysis of two partially overlapping regions – ASMIT (396 bp) & MORGAN (617 bp) – of the mitochondrial cytochrome oxidase subunit I (cox1). The Victorian sample exhibited greater nucleotide diversity, 1·4%vs. 1·0%, and a significant population partition appeared as barcodes did not cross-over between lakes. With one exception, Lake Albert populations were more mixed by sampled location, while those from Lake Victoria appeared more secluded. Using statistical parsimony, barcode ASMIT 1 was putatively ancestral to all others and analysis of MORGAN cox1 confirmed population diversity. All samples fell into two of five well-resolved lineages; sub-lineages therein broadly partitioning by lake. It seems that barcode ASMIT 1 (and close variants) was likely widely dispersed throughout the Nilotic environment but later diversifiedin situ, and in parallel, within Lake Albert and Lake Victoria. The genetic uniformity of UgandanS. mansonican no longer be assumed, which might better explain known epidemiological heterogeneities. While it appears plausible that locally evolved heritable traits could spread through most of the Lake Albert populations, it seems unlikely they could quickly homogenise into Lake Victoria or amongst populations therein.

Molecular characterization of closely related species in the parasitic genus Encarsia (Hymenoptera: Aphelinidae) based on the mitochondrial cytochrome oxidase subunit I gene

2005 ◽  
Vol 95 (5) ◽  
pp. 401-408 ◽  
Author(s):  
M.M. Monti ◽  
A.G. Nappo ◽  
M. Giorgini
Keyword(s):  
Cytochrome Oxidase ◽  
Cryptic Species ◽  
Related Species ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Closely Related Species ◽  
Mitochondrial Cytochrome Oxidase

AbstractThe genus Encarsia Förster includes parasitoid species that are effective natural enemies of whitefly and armoured scale insect agricultural pests. Within this genus, several species groups have been recognized on the basis of morphological similarity, although their monophyly appears uncertain. It is often difficult to separate morphologically similar species, and there is evidence that some species could in fact be complexes of cryptic species. Their correct identification is fundamental for biological control purposes. Recently, due to unreliability of morphological characters, molecular techniques have been investigated to identify markers that differentiate closely related species. In this study, DNA variation in an ~900 bp segment of the mitochondrial cytochrome oxidase subunit I (COI) gene was examined by both sequencing and PCR–RFLP. Two pairs of species that are difficult to distinguish morphologically were analysed: Encarsia formosa Gahan and Encarsia luteola Howard, belonging to the luteola group, and two populations of Encarsia sophia (Girault & Dodd) from Pakistan and Spain, belonging to the strenua group, recently characterized as cryptic species. High sequence divergence and species-specific restriction patterns clearly differentiate both species pairs. Parsimony analysis of the nucleotide sequences was also performed, including Encarsia hispida De Santis (luteola group) and Encarsia protransvena Viggiani (strenua group). Two monophyletic clades supporting the two groups of species considered were resolved. The results of this study support the use of the COI gene as a useful marker in separating species of Encarsia, for which morphological differences are subtle. Moreover, the COI gene appears potentially useful for understanding phylogenetic relationships in this genus.

scholarly journals Role of Chrysomya albiceps (Diptera: Calliphoridae) and Musca domestica (Diptera: Muscidae) Maggot Crop Contents in Identifying Unknown Cadavers

2020 ◽  
Author(s):  
Zainab Mohammad ◽  
Reem Alajmi ◽  
Mohammed Alkuriji ◽  
Dina Metwally ◽  
Walid Kaakeh ◽  
...  
Keyword(s):  
Musca Domestica ◽  
Coi Gene ◽  
Insect Larvae ◽  
Cytochrome Oxidase Subunit ◽  
Time Intervals ◽  
Specific Primers ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Chrysomya Albiceps ◽  
Mitochondrial Cytochrome Oxidase

Abstract Forensic entomology focuses on the analysis of insect larvae present at crime scenes to help identify unknown cadavers. Carrion-feeding maggots store food in a crop located at the anterior end of the gut. DNA recovered from the crop can be amplified, sequenced, and identified to determine the origin of the food. This information could help investigators to identify a missing victim if maggots are discovered at a crime scene in the absence of a corpse. Chrysomya albiceps (Wiedemann) and Musca domestica (Linnaeus) are primary forensic species. Little or no information on the identification of unknown cadavers using C. albiceps and M. domestica larvae is available, and we aimed to compare the effectiveness of using the crop contents of instars of C. albiceps and M. domestica larvae to identify corpses at different time intervals. Two hundred and forty larvae of both species were reared on rat and/or mouse liver, and DNA from crop contents was extracted after different time intervals from different instar larvae. DNA was amplified using specific primers that match the mitochondrial cytochrome oxidase subunit I (mt COI) gene of the food source. Results showed that food provided to larvae affected life span and the ability to be used to identify unknown cadavers. Chrysomya albiceps larvae proved more useful than M. domestica larvae. Moreover, crop contents of third instar larvae of both species fed for 24 h are more useful than contents from other time intervals in identifying unknown tissues. Results are promising and may help investigators to identify unknown/missing victims.

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