Molecular epidemiology ofSchistosoma mansoniin Uganda: DNA barcoding reveals substantial genetic diversity within Lake Albert and Lake Victoria populations

Parasitology ◽  
2009 ◽  
Vol 136 (13) ◽  
pp. 1813-1824 ◽  
Author(s):  
J. R. STOTHARD ◽  
B. L. WEBSTER ◽  
T. WEBER ◽  
S. NYAKAANA ◽  
J. P. WEBSTER ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Nucleotide Diversity ◽  
Lake Victoria ◽  
Population Diversity ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Statistical Parsimony ◽  
Mitochondrial Cytochrome Oxidase ◽  
Representative Samples

SUMMARYRepresentative samples of UgandanSchistosoma mansonifrom Lake Albert and Lake Victoria were examined using DNA barcoding, sequence analysis of two partially overlapping regions – ASMIT (396 bp) & MORGAN (617 bp) – of the mitochondrial cytochrome oxidase subunit I (cox1). The Victorian sample exhibited greater nucleotide diversity, 1·4%vs. 1·0%, and a significant population partition appeared as barcodes did not cross-over between lakes. With one exception, Lake Albert populations were more mixed by sampled location, while those from Lake Victoria appeared more secluded. Using statistical parsimony, barcode ASMIT 1 was putatively ancestral to all others and analysis of MORGAN cox1 confirmed population diversity. All samples fell into two of five well-resolved lineages; sub-lineages therein broadly partitioning by lake. It seems that barcode ASMIT 1 (and close variants) was likely widely dispersed throughout the Nilotic environment but later diversifiedin situ, and in parallel, within Lake Albert and Lake Victoria. The genetic uniformity of UgandanS. mansonican no longer be assumed, which might better explain known epidemiological heterogeneities. While it appears plausible that locally evolved heritable traits could spread through most of the Lake Albert populations, it seems unlikely they could quickly homogenise into Lake Victoria or amongst populations therein.

scholarly journals The Analysis of Morphological and Genetic Characteristics of Yellowstripe Scad from Muara Baru Modern Fish Market in North Jakarta

2020 ◽  
Vol 12 (2) ◽  
pp. 308
Author(s):  
Rahmat Sawalman ◽  
Hawis Madduppa
Keyword(s):  
Dna Barcoding ◽  
Molecular Analysis ◽  
Fish Species ◽  
Morphological Analysis ◽  
Coi Gene ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Fish Market ◽  
Commercially Important ◽  
Mitochondrial Cytochrome Oxidase

HighlightIdentification and morphological analysis of Selaroides leptolepis fish.Molecular analysis using the DNA Barcoding method with the COI gene (mitochondrial cytochrome oxidase subunit I).The number of base pairs in S. leptolepis from the sequencing results was 675bp.The morphological and molecular similarities of Selaroides leptolepis fishAbstractYellowstripe scad is one of the commercially important fish from the Carangidae family, which is marketed at Muara Baru Modern Fish Market, North Jakarta. In- formation regarding the presence of Selaroides leptolepis fish in the waters allows for effective conservation, and management of marine resources. A morphological identification of fish species is still considered inaccurate, so the molecular anal- ysis is necessary. This study aims to identify commercially important fish species deriving from Muara Baru Modern Fish Market, North Jakarta, employing mor- phological, and molecular analysis. A total of 30 specimens were collected from the field. The morphological analysis utilized a visual identification method, and morphometric measurement, while molecular analysis with DNA barcoding em- ployed the mitochondrial cytochrome oxidase subunit I (COI) gene. The results of the morphological analysis indicated that the fish species were yellowstripe scad (S. leptolepis). It also followed the results of molecular analysis of DNA barcoding that the fish was a S. leptolepis species. Therefore, the combination of morphological and genetic analysis has succeeded in identifying the fish species of S. leptolepis.

First rearing record and DNA sequence of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) associated with Saxifraga paniculata Miller from the Czech Republic

2020 ◽  
Vol 69 (3) ◽  
pp. 283-288
Author(s):  
Miloš Černý ◽  
Jiří Kocián ◽  
Jan Ševčík
Keyword(s):  
Czech Republic ◽  
Cytochrome Oxidase ◽  
Dna Sequence ◽  
Partial Sequence ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
The Czech Republic ◽  
Mitochondrial Cytochrome Oxidase

Abstract A male of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) was reared from a leaf mine at Saxifraga paniculata collected in Štramberk (Northern Moravia, Czech Republic). Partial sequence of the mitochondrial cytochrome oxidase subunit I (COI barcode region, 658 bp) is also provided.

Morphology and molecular phylogeny of ornamental freshwater prawns of the genus Macrobrachium (Decapoda, Caridea, Palaemonidae) from China with the description of a new species

Crustaceana ◽  
2021 ◽  
Vol 94 (10) ◽  
pp. 1201-1220
Author(s):  
Qinghua Chen ◽  
Wenjian Chen ◽  
Yuanwei Hu ◽  
Ka Yan Ma ◽  
Zhaoliang Guo
Keyword(s):  
New Species ◽  
Colour Pattern ◽  
Molecular Evidence ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Closely Related Species ◽  
Distribution Maps ◽  
Mitochondrial Cytochrome Oxidase ◽  
A New Species

Abstract This study dealt with three species of ornamental palaemonid freshwater prawns of the genus Macrobrachium, based on morphological and molecular analysis. Macrobrachium pentazona He, Gao & Guo, 2009; M. laevis Zheng, Chen & Guo, 2019; and M. bilineare sp. nov. are distinguishable from closely related species by segmental ratios, spination of the second pereiopods and the slender scaphocerite. Macrobrachium bilineare sp. nov. can easily be recognized in the field by its bright colour pattern. Molecular evidence of mitochondrial cytochrome oxidase subunit I (COI), also supports the characterization of this new species, raising the total number of Macrobrachium spp. known from China to 41. Detailed description, illustrations, colour photographs, habitat information, distribution maps and features of conservation significance are also briefly discussed.

scholarly journals Epimeria schiaparelli sp. nov., an amphipod crustacean (family Epimeriidae) from the Ross Sea, Antarctica, with molecular characterisation of the species complex  

Zootaxa ◽  
2007 ◽  
Vol 1402 (1) ◽  
pp. 23 ◽  
Author(s):  
ANNE-NINA LÖRZ ◽  
ELIZABETH W. MAAS ◽  
KATRIN LINSE ◽  
GRAHAM D. FENWICK
Keyword(s):  
Species Complex ◽  
Ross Sea ◽  
Similar Species ◽  
Molecular Characterisation ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Antarctic Species ◽  
Amphipod Crustacean ◽  
Colour Patterns ◽  
Mitochondrial Cytochrome Oxidase

Epimeria schiaparelli sp. nov. from the Ross Sea, Antarctica, is described in detail. The new species occurs on the shelf in 130–350 m depth. Epimeria schiaparelli can be distinguished from the most similar species, E. similis Chevreux, 1912 and E. macrodonta Walker, 1906 by a relatively short rostrum and a short second pereonite amongst other characters. Two distinct colour patterns are reported. Partial gene sequences of the mitochondrial cytochrome oxidase subunit I (COI) from 11 specimens of E. schiaparelli confirm that this species is new to science and closely related to E. similis, E. macrodonta and E. reoproi. The recent and historical separation of this Antarctic species is discussed. The syntypes of E. macrodonta consist of two species, so a lectotype is here designated.

Redefinition of Cornexcisia Fan & Kano, 2000 (Diptera: Sarcophagidae), with the first description of the female of Sarcophaga kurahashii (Shinonaga & Tumrasvin, 1979)

Zootaxa ◽  
2019 ◽  
Vol 4668 (3) ◽  
pp. 410-420
Author(s):  
CHAO WANG ◽  
YUNYUN GAO ◽  
THOMAS PAPE ◽  
DONG ZHANG
Keyword(s):  
Cytochrome Oxidase ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Gene Sequences ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Senior Synonym ◽  
First Time ◽  
Mitochondrial Cytochrome Oxidase

Sarcophaga Meigen, 1826 is proposed as a senior synonym of Cornexcisia Fan & Kano, 2000, syn. nov. and Fanzideia Xue, Verves & Du, 2011, syn. nov. Cornexcisia Fan & Kano, 2000, stat. rev. is given status as a subgenus and is considered a senior synonym of Fanzideia Xue, Verves & Du, 2011, syn. nov. at the subgeneric level. Cornexcisia is argued to contain S. (Cornexcisia) longicornuta (Fan & Kano, 2000), comb. nov., S. (C.) cygnocerca (Xue, Verves & Du, 2011), comb. nov., S. (C.) kurahashii (Shinonaga & Tumrasvin, 1979), subgen. comb. nov. (from Phallosphaera Rohdendorf) and S. (C.) suthep Pape & Bänziger, 2003, subgen. comb. nov. (from Rosellea Rohdendorf). Sarcophaga (C.) kurahashii is newly recorded from China (Yunnan), the male is redescribed and the female is described for the first time, supported by photographs, illustrations and mitochondrial cytochrome oxidase subunit I (COI) gene sequences. Species of Cornexcisia share an exceptionally long postpedicel in the female and the following apomorphic distiphallic appendages in the male: juxta ventro-proximally with an apically divided arm with cuticular pile, and lateral styli bifurcated from the base with each branch elongate, gently curved and slightly expanded apically. A key to the species of Cornexcisia is provided. 

Population biology and genetic diversity of two adjacent shrimp (Parapenaeopsis coromandelica) populations exploited under different fishing pressures in the coastal waters of Sri Lanka

2011 ◽  
Vol 92 (4) ◽  
pp. 819-829 ◽  
Author(s):  
M.D.S.T de Croos ◽  
Snæbjörn Pálsson
Keyword(s):  
Genetic Diversity ◽  
Sri Lanka ◽  
Population Biology ◽  
Gonadosomatic Index ◽  
Western Coast ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Routine Monitoring ◽  
Significant Divergence ◽  
Mitochondrial Cytochrome Oxidase

Parapenaeopsis coromandelica shrimp populations along the western coast of Sri Lanka have supported coastal trawling for the last hundreds of years. Two non-overlapping adjacent fishing grounds (Hendala and Negombo) sustain different fishing intensities. In order to obtain information on the population structure and genetic diversity of P. coromandelica in these two regions, differences in length–weight relationships, growth, spawning seasons, sex-ratios, gonadosomatic index, length at 50% maturity (L50) and sequence variation of the mitochondrial cytochrome oxidase subunit I were examined. Significant differences in population biology and genetic diversity were revealed from the two fishing grounds. Samples of Hendala showed lower L50 and genetic diversity which are considered as potential effects and symptoms of extensive selective harvesting. Further, the shrimps' behaviour seems to be triggering the separation through low mixing of individuals at the two fishing grounds resulting in significant divergence based on haplotype frequencies. Management of P. coromandelica should consider the revealed biological and genetic evidences on existence of two sub-populations/stocks together with a routine monitoring of genetic effects due to harvesting.

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