PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM KUNING TELUR TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN

The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,5% green tea extract in skim milk and egg yolk diluent, 0,1% green tea extract in skim milk and egg yolk diluent, and 0,15% green tea extract in skim milk and egg yolk diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after being diluted. The data obtained was analyzed with the Analysis of Variant (ANOVA), followed by multiple range test duncan. The results showed that the highest percentage of motility, viability and intact plasma membrane derived from green tea extract 0,15% on skim milk and egg yolk diluent for 1 day storage is 86.66b ± 2.58, 92.00b ± 1.78, and 74.16b ± 3.25. The lowest percentage of motility, viability and intact plasma membranes was obtained from the skim milk and egg yolk diluent for 5 days storage is 43.33a ± 6.05, 56.50a ± 3.08 and 28.33a ± 2.80. The conclusion of this study is the addition of 0.15% green tea extract in a skim milk and egg yolk diluent can maintain the quality of sperm for up to 5 days of storage at cold temperatures.

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PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN

Ovozoa Journal of Animal Reproduction ◽

10.20473/ovz.v8i2.2019.127-131 ◽

2020 ◽

Vol 8 (2) ◽

pp. 127

Author(s):

Itrah Khoirunnisa ◽

Suherni Susilowati ◽

Lilik Maslachah ◽

Tri Wahyu Suprayogi ◽

Rochmah Kurnijasanti ◽

...

Keyword(s):

Plasma Membrane ◽

Green Tea ◽

Analysis Of Variance ◽

Camellia Sinensis ◽

Egg Yolk ◽

Green Tea Extract ◽

Cold Temperature ◽

Tea Extract ◽

Range Test

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.

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PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN

Ovozoa Journal of Animal Reproduction ◽

10.20473/ovz.v8i2.2019.116-121 ◽

2020 ◽

Vol 8 (2) ◽

pp. 116

Author(s):

Maulinda Agchirilia Vladika ◽

Sunaryo Hadi Warsito ◽

Suherni Susilowati ◽

Tatik Hernawati ◽

Rochmah Kurnijasanti ◽

...

Keyword(s):

Plasma Membrane ◽

Green Tea ◽

Analysis Of Variance ◽

Camellia Sinensis ◽

Sperm Quality ◽

Skim Milk ◽

Green Tea Extract ◽

Tea Extract ◽

One Way Anova

The purpose of this study was to determine the effect and the best concentrasion of green tea extract in skim milk diluent for motility, viability, and intact plasma membrane Sapudi sheep spermatozoa that was stored 50C. The semen was divided into four groups: skim milk diluent; 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Spermatozoa quality was observed first day until fifth day after diluent. The data obtained was analyzed with the analysis of variance one way ANOVA. The result showed that the percentage of motility, viability and intact plasma membrane spermatozoa at the 5th day after diluent showed a significant differece (p<0,05) between 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Motility at the 3th day after diluent respectively 46,67b ± 2,582; 43,33bc ± 4,028; 40,00ab ± 3,162; and 37,50a ± 2,739. Viability at the 3th day after diluent respectively are 79,50b ± 1,643; 77,67b ± 3,327; 77,00ab ± 3,230; and 73,83a ± 3,061. Intact plasma membrane at the 3th day after diluent respectively are 50,17b ± 2,483; 47,00ab ± 3,899; 46,83ab ± 2,858; and 46,00a ± 3,286. The conclusion of this study was the green tea extract of 0,05 mg in milk skim diluent could increase the percentage of sperm quality until 5th day after diluent.

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Penambahan alfa-tokoferol dalam pengencer susu skim - kuning telur terhadap kualitas spermatozoa domba Sapudi yang disimpan pada suhu 5°C

Ovozoa Journal of Animal Reproduction ◽

10.20473/ovz.v9i3.2020.69-76 ◽

2020 ◽

Vol 9 (3) ◽

pp. 69

Author(s):

Satya Alysa Cahya Puspita ◽

Suherni Susilowati ◽

Trilas Sardjito ◽

Abdul Samik ◽

Indah Norma Triana ◽

...

Keyword(s):

Plasma Membrane ◽

Membrane Integrity ◽

Skim Milk ◽

Egg Yolk ◽

Alpha Tocopherol ◽

Control Treatment ◽

Control Group ◽

Cold Temperatures ◽

Fresh Semen

Spermatozoa in fresh semen of Sapudi ram has a limited life span. The storage of semen in cold temperatures (5 °C) is intended to prolong the spermatozoa's life. However, storage in cold temperatures can lead to increased production of reactive oxygen species (ROS). This condition reduces the quality of spermatozoa. The purpose of this study was to determine the effect of alphatocopherol supplementation in skim milk-egg yolk extender on viability, motility, and plasma membrane integrity of Sapudi ram spermatozoa. Fresh semen derived from Sapudi ram was divided into four treatment groups. Control treatment (P0): semen was added in the extender of skim milkegg yolk without alpha-tocopherol. Three other treatments: P1, P2, and P3 semen were added in skim milk-egg yolk extender with the supplementation of 0.25, 0.5, and 1 gram alpha-tocopherol/ 100 mL extender, respectively. The results showed that the viability, motility, and integrity of the spermatozoa plasma membrane decreased gradually according to the storage length. Supplementation of skim milk-egg yolk extender with 0.5 gram of alpha-tocopherol/100 mL (P2) was able to maintain spermatozoa quality longer (p <0.05) than the control group. It can be concluded that alpha-tocopherol with a concentration of 0.5 g/100 mL of skim milk-egg yolk extender effectively maintains the quality of Sapudi ram spermatozoa in storage at 5 ° C.

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Effect of green tea extract, ginger essential oil and nanofibrillated cellulose reinforcements in starch films on the keeping quality of strawberries

Journal of Food Processing and Preservation ◽

10.1111/jfpp.16109 ◽

2021 ◽

Author(s):

Renette Rodrigues ◽

Sharmila Patil ◽

Jyoti Dhakane‐Lad ◽

Vigneshwaran Nadanathangam ◽

Archana Mahapatra

Keyword(s):

Essential Oil ◽

Green Tea ◽

Nanofibrillated Cellulose ◽

Green Tea Extract ◽

Keeping Quality ◽

Starch Films ◽

Tea Extract

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Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

Animal Bioscience ◽

10.5713/ajas.20.0025 ◽

2021 ◽

Vol 34 (2) ◽

pp. 198-204 ◽

Cited By ~ 1

Author(s):

Suherni Susilowati ◽

Trilas Sardjito ◽

Imam Mustofa ◽

Oky Setio Widodo ◽

Rochmah Kurnijasanti

Keyword(s):

Pregnancy Rate ◽

Dna Fragmentation ◽

Green Tea ◽

Prostaglandin F2α ◽

Green Tea Extract ◽

Control Group ◽

Bull Semen ◽

Frozen Semen ◽

Tea Extract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

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