scholarly journals PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI

2020 ◽  
Vol 8 (2) ◽  
pp. 139
Author(s):  
Mayrena Ayu Cesaria ◽  
A.T.Soelih Estoepangestie ◽  
Suherni Susilowati ◽  
Tatik Hernawati ◽  
Sri Pantja Madyawati ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Egg Yolk ◽  
Coconut Water ◽  
Good Effect ◽  
Storage Duration ◽  
Significance Level ◽  
Chicken Egg ◽  
Significant Difference ◽  
Chicken Egg Yolk ◽  
And Storage

This study aimed to determine the influence of mixture diluter of chicken egg yolk and young coconut water on the integrity of plasma membrane and abnormalities of Sapudi sheep spermatozoa. The experimental design used in this study was a complete random method. The data was analysed using ANOVA, and if there is a significant difference then proceed with Duncan’s Multiple Range Test ( DMRT ) at significance level of 5%. Based on the result of the research, the influence of mixture diluter of chicken egg yolk and young coconut water was not significantly different ( p > 0,05 ) on the intact plasma membrane of spermatozoa on the first day until the fourth day. Interaction between treatment and storage duration had no effect on spermatozoa abnormality on the first day, second day and the fourth day. It can be concluded that mixture diluter of chicken egg yolk and young coconut water had a good effect on the plasma membrane and spermatozoa abnormalities up to the third day.

scholarly journals EVALUATION OF CULTURE SUBSTRATE FOR THE ISOLATION AND CULTIVATION OF LEGIONELLA WITH THE USE OF THE STRAINS ISOLATED DURING THE XXII OLYMPIC GAMES-2014 IN SOCHI

2017 ◽  
pp. 49-50
Author(s):  
N.V. Zharinova ◽  
E.B. Zhilchenko ◽  
L.S. Katunina ◽  
O.A. Konyaeva ◽  
N.S. Serdyuk ◽  
...  
Keyword(s):  
Experimental Evaluation ◽  
Olympic Games ◽  
Egg Yolk ◽  
Enzymatic Hydrolysate ◽  
Long Period ◽  
Chicken Egg ◽  
Environmental Objects ◽  
Culture Substrate ◽  
Chicken Egg Yolk ◽  
And Storage

The article presents the results of experimental evaluation of the effectiveness of new culture substrate developed for the isolation and cultivation of Legionella, the strains isolated from environmental objects in the resort city of Sochi before and during XXII Olympic games. Dense nutrient substrate prepared on the basis of enzymatic hydrolysate of chicken egg yolk and enzymatic hydrolysate of the lung of the pig with additional stimulants allowed saving number of colonies in the earlier stages of the study and storage of the isolated strains during long period of time compared with domestic commercial nutrient substrate.

38 QUAIL EGG YOLK IN CITRATE EXTENDER IS SUITABLE FOR CRYOPRESERVATION OF NGUNI BULL SEMEN

2016 ◽  
Vol 28 (2) ◽  
pp. 149
Author(s):  
M. M. Seshoka ◽  
M. L. Mphaphathi ◽  
K. S. Mafolo ◽  
M. Nkadimeng ◽  
Z. C. Raphalalani ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Egg Yolk ◽  
Sperm Analysis ◽  
Chicken Egg ◽  
Bull Semen ◽  
Significant Difference ◽  
Computer Aided ◽  
Chicken Egg Yolk ◽  
Fresh Semen ◽  
Motility Rate

Traditionally, commercial hen egg yolk has been used in extenders or freezing media because of its easy availability. However, the use of quail egg yolk has not been used for preserving Nguni bull semen. The aim of the study was to compare the suitability of different quail egg yolk concentrations (5, 10, 15, and 20%) for cryopreserving Nguni bull semen. Semen was collected from 14 stud Nguni bulls with the aid of electro ejaculator. Collected semen samples were kept in a thermos-flask containing warm water at a temperature of 37°C and transported to the laboratory for further analyses. The sperm motility traits were evaluated using computer-aided sperm analysis prior extension. Semen samples were then randomly allocated into 5, 10, 15, and 20% of quail egg yolk and 20% concentration of chicken egg yolk (control) in citrate extender. The extender was supplemented with 12% of glycerol (Seshoka et al. 2012) as a cryoprotectant, and semen samples were diluted (1 : 2) and equilibrated for a period of 4 h at 5°C. After equilibration, semen samples were loaded into 0.25-mL straws, placed into a controlled rate programmable freezer, and stored in a LN tank (–196°C) until thawing. Frozen semen straws were thawed in a water bath at 37°C for 1 min. Thawed semen was evaluated for sperm motility traits using a computer-aided sperm analysis system. Data were analysed with ANOVA. A significant difference was recorded between fresh total sperm motility rate (99%) and frozen-thawed semen samples with either 5% (87.3%) quail or 20% (87.6%) chicken egg yolk citrate extender compared with 10% (92.6%), 15% (91.2%), or 20% (89.9%) quail egg yolk citrate extender. Moreover, fresh semen also resulted in a significantly higher progressive sperm motility rate (39.3%) as compared with frozen-thawed with 5% (26.2%) or 20% quail (28.5%) or 20% chicken (22.7%) egg yolk citrate extender. The results also demonstrated that the use of 10, 15, and 20% quail egg yolk in citrate extender yielded comparable results on total sperm motility with fresh semen as compared with 5% quail and 20% chicken egg yolk. In conclusion, quail egg yolk extender provided sufficient cryo-effectiveness to cryopreservation of Nguni bull semen.

scholarly journals Daya Hidup Spermatozoa Epididimis Sapi Persilangan yang Dipreservasi dengan Air Kelapa Muda pada Suhu 5oC (VIABILITY OF EPIDIDYMAL SPERMATOZOA CROSSBREED CATTLE PRESERVED WITH COCONUT WATER AT 5oC)

2018 ◽  
Vol 18 (4) ◽  
pp. 571
Author(s):  
Muhammad Rizal ◽  
Muhammad Riyadhi ◽  
Bambang Irawan ◽  
Anis Wahdi ◽  
Habibah Habibah ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Saline Solution ◽  
Egg Yolk ◽  
Coconut Water ◽  
Randomized Design ◽  
Significant Difference ◽  
Epididymal Spermatozoa ◽  
Completely Randomized Design ◽  
Difference Test

The objective of this research was to examine the effectiveness of coconut water with various egg yolk concentrations in maintaining the viability of epididymal spermatozoa of crossbreed cattle preserved at 5oC. Five testis with epididymides of crossbreed cattle were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure methods of cauda epididymides with saline solution (0.9% NaCl). Collected-spermatozoa was equally divided in volume into four tubes and diluted with lactose extender containing 20% egg yolk (control), 90% coconut water + 10% egg yolk (CWEY10), 85% coconut water + 15% egg yolk (CWEY15), and 80% coconut water + 20% egg yolk (CWEY20), repectively. Diluted-spermatozoa was stored in refrigerator at 5oC. Quality of dilutedspermatozoa including percentages of motile spermatozoa (MS), live spermatozoa (LS), spermatozoa with intact plasma membrane (IPM) were evaluated every day during four days of storage. Data were analyzed by using completely randomized design with four treatments and five replicates. Means were compared with significant difference test at 0.05 significant level. Results of this study showed that mean of spermatozoa concentration, percentage of MS, percentage of LS, percentage of spermatozoa abnormal, and percentage of IPM of crossbreed cattle fresh epididymal spermatozoa were 1,414 million cell/ml, 72%, 85%, 9%, and 90%, respectively. At day-4 of the storage, percentages of MS, LS, and IPM of control (43, 52.2, 59.2%) and CWEY20 (42, 52, 59%) were significantly (P<0.05) higher than CWEY10 (33, 45.4, 52.8%) and CWEY15 (37, 50, 54.6%). In conclusion, lactose and CWEY20 extenders could be maintaining the quality of epidydimal spermatozoa of crossbreed cattle for three days preservation at 5oC and two days for CWEY10 and CWEY15.

scholarly journals A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C

2019 ◽  
Vol 12 (7) ◽  
pp. 1116-1120 ◽  
Author(s):  
Siti Eliana Rochmi ◽  
Miyayu Soneta Sofyan
Keyword(s):  
Sperm Quality ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Value ◽  
Coconut Water ◽  
Sperm Viability ◽  
Spermatozoa Motility ◽  
Chicken Egg ◽  
Semen Volume ◽  
Chicken Egg Yolk

Aim: The present study was conducted to evaluate the quality of rooster sperm at 5°C after treatment with a diluent containing coconut water, fructose, and chicken egg yolk and stored the semen sample at 5°C. Materials and Methods: Ten semen samples from 10 healthy roosters were subjected to four different treatments. For the treatments, 0.2 ml fresh semen with a sperm concentration of 5.2X109 cell/ml was mixed with T0 (no diluent), T1 (0.34 ml coconut water and 6 μl fructose), T2 (0.274 ml coconut water, 0.12 ml egg yolk, and 6 μl fructose), and T3 (0.34 ml egg yolk and 6 μl fructose) solutions. Each treated solution was stored at 5°C and evaluated both macroscopically and microscopically. Macroscopically, semen volume, pH, and sperm concentration were evaluated. The microscopic sperm characteristics examined included total motility (i.e., rapid, medium, or slow), progressive and non-progressive motility, viability, and spermatozoa abnormalities noted at different storage times. The results showed that spermatozoa motility was under 40%. Results: The results indicated that sperm viability significantly affected (p<0.05). The highest mean value of sperm viability on day 7 of storage was found after treatment with the T2 solution (46.100±0.5677%). Similarly, spermatozoa abnormalities were significantly lower after treatment with the T2 solution (6.680±1.702%). Conclusion: The addition of a diluent containing coconut water, egg yolk, and fructose helped in the better preservation spermatozoa motility, as well as viability for up to 7 days when the semen samples were stored at 5°C.

scholarly journals Comparative study of chicken egg yolk and quail egg yolk in two chilled canine semen extenders

2020 ◽  
Vol 17 (4) ◽  
pp. 62-69
Author(s):  
S.A. Ubah ◽  
M. Sule ◽  
I.C. Chibuogwu ◽  
P.K. Columbus ◽  
K.O. Abah ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Egg Yolk ◽  
Chicken Egg ◽  
Significant Difference ◽  
Skimmed Milk ◽  
Mass Activity ◽  
The Difference ◽  
Chicken Egg Yolk

The aim of this work was to substitute chicken egg yolk with quail egg yolk in two semen extenders and to evaluate the quality of the extended canine semen following chilled storage. Semen was pooled from male dogs (n= 4) of about 18-months old and body weight of about 28 kg. Four extenders were tested: (1) tris buffered chicken egg yolk extender (2) tris buffered quail egg yolk extender, (3) skimmed milk chicken egg yolk extender and (4) skimmed milk quail egg yolk extender. Semen was diluted with corresponding extender in the ratio 1:4. The diluted semen samples were analyzed for motility, mass activity, viability, abnormalities percentage and pH for three consecutive days. There was no significant difference (P>0.05) between chicken egg yolk and quail egg yolk in either tris diluent or skimmed milk extender with respect to pH, mass activity and sperm motility. Samples stored in both the tris and skimmed milk-based extenders with quail egg yolk displayed greater viability than those in chicken egg yolk but the difference was not significant (P>0.05). Viability, mass activity and sperm motility decreased as treatment days increased in both chicken and quail egg yolk extenders. Results showed that a pH of 6.5 was maintained from day 0 to day 3. There was no difference in semen quality between chicken and quail egg yolk in either the tris diluent or skimmed milk extender (P> 0.05). It was recommended that quail egg yolk could be substituted for chicken egg yolk in the two canine semen extenders. Further modifications of the diluents with quail egg yolk might produce an improved result. Keywords: Canine, Chicken Chilled, Egg yolk, Extenders, Quail, Semen

Chicken egg yolk antibodies (IgY)-based antivenom for neutralization of snake venoms: a review

Toxin Reviews ◽  
2021 ◽  
pp. 1-12
Author(s):  
Ankit Choraria ◽  
Rajeswari Somasundaram ◽  
S. Janani ◽  
Selvakumar Rajendran ◽  
Naoual Oukkache ◽  
...  
Keyword(s):  
Egg Yolk ◽  
Snake Venoms ◽  
Chicken Egg ◽  
Egg Yolk Antibodies ◽  
Chicken Egg Yolk

Development of viper-venom antibodies in chicken egg yolk and assay of their antigen binding capacity

Toxicon ◽  
2002 ◽  
Vol 40 (7) ◽  
pp. 857-861 ◽  
Author(s):  
C Maya Devi ◽  
M Vasantha Bai ◽  
L.K Krishnan
Keyword(s):  
Binding Capacity ◽  
Egg Yolk ◽  
Antigen Binding ◽  
Chicken Egg ◽  
Chicken Egg Yolk

Isolation of Immunoglobulin from Chicken Egg Yolk using Single-Stage Ultrafiltration with 100-kDa Regenerated Cellulose Membranes

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jianguo Liu ◽  
Juan Yang ◽  
Hai Xu ◽  
Hu Zhu ◽  
Jianbo Qu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Egg Yolk ◽  
Single Stage ◽  
Regenerated Cellulose ◽  
Cellulose Membrane ◽  
Permeate Flux ◽  
Operational Parameters ◽  
Solution Ph ◽  
Chicken Egg ◽  
Chicken Egg Yolk

The aim of this work is to develop a membrane-based cost-effective process for the rapid isolation of immunoglobulin from chicken egg yolk. It was found that a single-stage ultrafiltration using a 100 kDa molecular weight cut-off regenerated cellulose membrane could be employed to isolate immunoglobulin from the crude feedstock. The effects of operational parameters (solution pH, ionic strength, stirring speed and permeate flux) on the transmission of immunoglobulin and the presence of impurity protein with molecular weight close to immunoglobulin were quantified using the parameter scanning ultrafiltration technique. Under optimized conditions, the purity of immunoglobulin obtained was about 85 percent after the single-stage ultrafiltration process, and the recovery of immunoglobulin from the feedstock was 91 percent.

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