Changes in serum antibody titer following rabies vaccination in the giant panda

Background: Studies reporting the long-term humoral response after receiving the BNT162b2 COVID-19 vaccine are important to drive future vaccination strategies. Yet, available literature is scarce. Covidiagnostix is a multicenter study designed to assess the antibody response in >1000 healthcare professionals (HCPs) who received the BNT162b2 vaccine. Methods: Serum was tested at time-0 (T0), before the first dose, T1, T2, and T3, respectively, 21, 42, and 180 days after T0. Antibodies against the SARS-CoV-2 nucleocapsid-protein were measured to assess SARS-CoV-2 infections, whereas antibodies against the receptor-binding domain of the spike protein were measured to assess the vaccine response. Neutralization activity against the D614G, B.1.1.7, and B.1.351 variants were also analyzed. Results: Six months post-vaccination HCPs showed an antibody titer decrease of approximately 70%, yet, the titer was still one order of magnitude higher than that of seropositive individuals before vaccination. We identified 12 post-vaccination infected HCPs. None showed severe symptoms. Interestingly, most of them showed titers at T2 above the neutralization thresholds obtained from the neutralization activity experiments. Conclusion: Vaccination induces a humoral response which is well detectable even six months post-vaccination. Vaccination prevents severe COVID-19 cases, yet post-vaccination infection is possible even in the presence of a high anti-S serum antibody titer.

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Novel malignant-mesothelioma-associated antigens (Gene-X and THBS-2) in the diagnosis of malignant pleural mesothelioma (MPM).

Journal of Clinical Oncology ◽

10.1200/jco.2012.30.15_suppl.10585 ◽

2012 ◽

Vol 30 (15_suppl) ◽

pp. 10585-10585

Author(s):

Fumihiro Tanaka ◽

Yoshiki Shigematsu ◽

Takeshi Hanagiri ◽

Hidetaka Uramoto ◽

Tomoko So ◽

...

Keyword(s):

Antibody Titer ◽

Malignant Pleural Mesothelioma ◽

Early Stage ◽

Asbestos Exposure ◽

Serum Antibody ◽

Diagnostic Value ◽

Pleural Mesothelioma ◽

Roc Curve Analysis ◽

Antibody Titers ◽

Serum Antibody Titer

10585 Background: Malignant pleural mesothelioma (MPM) is a highly aggressive malignant tumor of the pleura associated with asbestos exposure, and its diagnosis is usually difficult at early stage. We identified novel mesothelioma-related antigens, Gene-X and thrombospondin-2 (THBS-2), recognized by tumor-infiltrating B cells (Cancer Sci 2009), but the clinical significance in the diagnosis of MPM remains unclear. Methods: A total of 120 patients, who presented with a suspicion of MPM and received pleural biopsy, were reviewed; 97 patients were finally diagnosed with MPM and 27 were with non-malignant diseases (NM). The antibody-titers against Gene-X and THBS-2 in the sera were measured by ELISA method. Results: The serum antibody-titer against THBS-2 was significantly higher in MPM patients than in NM (P<0.01), but there was no difference in the serum antibody-titer against Gene-X (Table). The receiver operating characteristic (ROC) curve analysis showed a significant diagnostic value of serum antibody-titer against THBS-2 with the area-under curve of 0.886 (95% CI, 0.797 - 0.975; P<0.001) in discrimination of MPM from NM diseases; the sensitivity and specificity, when the cut-off value was 0.08, were 72.2% and 95.5%, respectively. Conclusions: The serum antibody-titer against THBS-2 can be a useful non-invasive marker in the diagnosis of MPM. [Table: see text]

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Lethal Encephalitis in 14-Day-Old Mice Given Avirulent Semliki Forest Virus

Veterinary Pathology ◽

10.1177/030098587801500315 ◽

1978 ◽

Vol 15 (3) ◽

pp. 393-399 ◽

Cited By ~ 1

Author(s):

M. Chew-Lim

Keyword(s):

Antibody Titer ◽

Semliki Forest Virus ◽

Serum Antibody ◽

Pyramidal Cells ◽

Old Mice ◽

Serum Antibody Titer

Avirulent Semliki Forest Virus when inoculated intracerebrally and intraperitoneally into 14-day-old Swiss A2G mice induced a lethal encephalitis. There was destruction of neurones, especially pyramidal cells of the hippocampus, despite a rising serum antibody titer.

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The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken

Bioscience Reports ◽

10.1042/bsr20190224 ◽

2019 ◽

Vol 39 (5) ◽

Author(s):

Haifeng Yang ◽

Ping Zhang ◽

Xiaozhou Xu ◽

Xiaolan Chen ◽

Qingxin Liu ◽

...

Keyword(s):

Antibody Titer ◽

Newcastle Disease ◽

Lymphocyte Proliferation ◽

Serum Antibody ◽

Physiological Saline ◽

Interferon Γ ◽

Paulownia Tomentosa ◽

Ifn Γ ◽

Immune Efficacy ◽

Serum Antibody Titer

Abstract The extracts of Paulownia tomentosa (P. tomentosa) exhibit multiple pharmacological activities. In the present study, P. tomentosa flower polysaccharides (PTFP) were extracted by water decoction and ethanol precipitation, and the immunologic modulations of PTFP against Newcastle disease (ND) vaccine was investigated in chickens. The results showed that in a certain range of concentrations, PTFP treatment can dose-dependently enhance lymphocyte proliferation. Then, 280 14-days-old chickens were randomly divided into seven groups, and vaccinated with ND vaccine except blank control (BC) group. At the first vaccination, chickens were orally administrated with PTFP at concentration ranging from 0 to 50 mg/kg once a day for 3 successive days, and the BC group was treated with physiological saline. The lymphocyte proliferation rate, serum antibody titer, and levels of interferon-γ (IFN-γ) were respectively measured on 7, 14, 21, and 28 days after the first vaccination. The results showed that PTFP at the suitable doses could significantly promote lymphocyte proliferation, enhance serum antibody titer, and improve serum IFN-γ concentrations. Taken together, these data indicated that PTFP could improve the immune efficacy against ND vaccine in chickens, and could be as the candidate of a new-type immune adjuvant.

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Astragalus Polysaccharides Enhance the Immune Response to Newcastle Disease Virus Vaccination in Chickens

Journal of University of Shanghai for Science and Technology ◽

10.51201/jusst12544 ◽

2021 ◽

Vol 23 (1) ◽

Author(s):

Naji A .I . ◽

◽

Huda S.J ◽

Keyword(s):

Antibody Titer ◽

Normal Saline ◽

Sandwich Elisa ◽

Serum Antibody ◽

Elisa Test ◽

Rt Pcr ◽

High Concentration ◽

Serum Samples ◽

Astragalus Polysaccharide ◽

Serum Antibody Titer

This study was conducted to evaluated the effects of Astragalus polysaccharide on immune responses of chickens immunized with NDV Lasota vaccine. One hundred chickens at one-day-old( Ross breed) were brought , on five day of age, the average maternal serum Abs was measured by hemaglutination inhibition (HI) test and the titer was 3.43 log, and then divided into five equal groups in each group twenty chicks and on day 10 of age the Astragalus polysaccharide high concentration (APSH) group were given 0.5 ml at 400mg /100ml , the Astragalus polysaccharide medium concentration (APSM )group were given 0.5 ml at 200mg /100ml, the Astragalus polysaccharide low concentration (APSL) group were given 0.5 ml at 100mg /100ml , Vaccine control (VC) group were given 0.5 ml normal saline only, Negative control (NC ) group were given normal saline only , all groups orally administrated for four days. At 14 day of age, all chickens with the exception of NC group , were vaccinated with ND Lasota by Intraocular and Intranasal methods. Four blood sample from all groups were aspirated from jugular vein at 0h (before vaccination),6h,12h and 24h(after vaccination ) for determine of Chicken IL-6(Interleukin 6) by RT-PCR. On day 20and 30 of age after vaccination , three chicken were sampled randomly from each group to detect specific serum Antibody titer of NDV by HI test. On days 1,7,14,21after vaccination, four serum Samples from the same of chicken to determines IgA level by sandwich ELISA test. The cellular and humeral response including the production of cytokine IL-6 ( by RT-PCR test) were measured on 0h(before vaccination),6h,12h and 24h( after vaccination )and IgA antibody ( by ELISA test ) were measured on 1d,7d,14d,and 21days after vaccination and serum antibody titer Specific to NDV( by HI test ) were measured at age 20, and 30 days after vaccination evaluated by series of experiments. Results revealed that all the polysaccharide groups were numerically increased in antibody levels, the expression of IL-6and IgA level ,but three parameter were significant (𝑃 < 0.05) in the APSH group compared to corresponding (VC) vaccinated group and( NC) non –vaccinated group. These results suggest that orally administered APS could significantly enhance the efficacy of NDV vaccination and has important implications for the further use of APS as a novel adjuvant.

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