scholarly journals Detection of bartonella DNA in ixodid ticks, mosquitoes and in patients blood in Novosibirsk region

2006 ◽  
Vol 5 ◽  
pp. 106-110
Author(s):  
O. V. Morozova ◽  
L. V. Petrozhitskaya ◽  
N. Ya. Chernousova ◽  
A. G. Mirzayeva ◽  
A. K. Dobrotvorsky ◽  
...  
Keyword(s):  
Human Blood ◽  
Mixed Infection ◽  
Nested Pcr ◽  
Bartonella Henselae ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Ixodes Persulcatus ◽  
Bartonella Quintana ◽  
Novosibirsk Region ◽  
Pcr Product

Bartonella DNA was detected using nested PCR in ticks Ixodes persulcatus and Dermacentor reticulatus, in mosquitoes Aedes cantans, but not in other mosquito or gnat species. Phylogenic analysis of the PCR product nucleotide sequences proved the infec- tion of arthropod vectors and human blood with Bartonella henselae, Bartonella quintana or their mixed infection in Novosibirsk region.

scholarly journals INFLUENCE OF NATURAL FACTORS ON THE NUMBER OF IXODIC TICKS OF THE GENUS DERMACENTOR IN TYUMEN REGION

2021 ◽  
pp. 156-160
Author(s):  
Glazunov
Keyword(s):  
Air Temperature ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Ixodes Persulcatus ◽  
Large Mammals ◽  
Depressing Effect ◽  
Dominance Index ◽  
Spring Period ◽  
Natural Factors ◽  
Ixodes Apronophorus

At present, the ixodofauna of the Tyumen region is represented by six species of ixodid ticks – these are Ixodes persulcatus Schulze, 1930; Dermacentor reticulatus Fabricius, 1794 (D. pictus, Hermann, 1804); D. marginatus Sulz, 1776; I. apronophorus P. Sch., 1924; I. (Exopalpiger) trianguliceps Bir., 1895 and I. (Ceratixodes) plumbeus, Kirsch., 1936. Three species of ixodid ticks parasitize cattle: I. persulcatus, D. reticulatus, D. marginatus. The remaining three species of ticks, Ixodes apronophorus, Ixodes strianguliceps, and Ixodes plumbeus, belong to the nest-like parasitic type and therefore are less dangerous parasites for large mammals. The most widespread ticks of the genus Ixodes and Dermacentor in the context of all natural-geographical zones of the region are ticks of the genus Dermacentor, their dominance index was 54.8%. As a result of observation, it was noted that the temperature and humidity of the air affect the activity of ixodid ticks of the genus Dermacentor in nature. Thus, the peak of ixodid activity in the spring period fell on days when the air temperature reached values from 16 oC to 26 oC, and in autumn from 15 oC to 21 oC, while lower and higher humidity indices had a depressing effect on ixodid mites of the genus Dermacentor.

scholarly journals Выявление различных видов риккетсий у иксодовых клещей, в крови людей и мелких млекопитающих на юге Западной Сибири и на Урале

2006 ◽  
Vol 5 ◽  
pp. 121-125
Author(s):  
Ya. P. Igolkina ◽  
N. V. Fomenko ◽  
N. N. Livanova ◽  
V. B. Astanin ◽  
L. A. Gosteeva ◽  
...  
Keyword(s):  
Small Mammals ◽  
Human Blood ◽  
Nested Pcr ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Rickettsia Helvetica ◽  
Rickettsia Slovaca ◽  
High Prevalence ◽  
Human Blood Samples ◽  
Rickettsia Sibirica

We investigated ticks, human blood samples, specimens of tissue and blood from small mammals using nested PCR. We de- tected high prevalence Rickettsia tarasevichiae and Rickettsia sp. RpA4 in samples of ticks Ixodes persulcatus and Dermacentor re- ticulatus, correspondingly. We observed DNA of pathogenic Rickettsia helvetica and Rickettsia slovaca in I. persulcatus and Der- macentor marginatus and DNA of Rickettsia sibirica in human blood and in blood and liver samples of small mammals.

scholarly journals Distribution of the tick-borne encephalitis virus among naturally infected ixodid ticks and small mammals in the Novosibirsk region

2015 ◽  
Vol 20 (4) ◽  
pp. 26-34
Author(s):  
V. N Bakhvalova ◽  
G. S Chicherina ◽  
V. V Panov ◽  
V. V Glupov ◽  
O. V Morozova
Keyword(s):  
Virus Infection ◽  
Common Shrew ◽  
Far East ◽  
Carrier State ◽  
Ixodid Ticks ◽  
Ixodes Persulcatus ◽  
Frequency Detection ◽  
Novosibirsk Region ◽  
Tick Borne Encephalitis ◽  
Ixodes Pavlovskyi

A comparative analysis of the virus infection carrier state and distribution of genetic types of the virus of tick-borne encephalitis (TBE) among invertebrates (2 species of ticks, Ixodes persulcatus Schulze and Ixodes pavlovskyi Pomerantsev 1946) and vertebrate reservoir hosts (4 species of small rodents: the red vole Myodes rutilus Pallas, gray-sided vole Myodes rufocanus Sundevall, field mouse Apodemus agrarius Pallas, birch mice Sicista betulina Pallas and 1 species of insectivores - common shrew Sorex araneus L (1758)), dominating on the territory of the Novosibirsk region in 2009-14 years, was performed with the use of virological and molecular biological methods. Frequency detection of RNA and / or E protein in mammals (70,9 ± 3,0%) were shown to considerably exceed levels of virus infection carrier state rate of ticks (3,4 ± 0,4). In the circulation of three major types of TBE - Far East (FE), Siberian (NIB) and European (Eur) in natural populations in mono - or polytype forms in mites Sib type prevailed (p

scholarly journals Detection and genotyping of Anaplasma phagocytophilum in I. persulcatus and D. reticulatus ticks collected in Tomsk (Western Siberia) in 2015–2016

2019 ◽  
Vol 18 (2) ◽  
pp. 89-98
Author(s):  
M. Yu. Kartashov ◽  
T. P. Mikryukova ◽  
N. S. Moskvitina ◽  
E. I. Krivosheina ◽  
A. I. Kuznetsov ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Nucleotide Sequence ◽  
Western Siberia ◽  
Genetic Material ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Ixodes Persulcatus ◽  
Human Granulocytic Anaplasmosis ◽  
Granulocytic Anaplasmosis ◽  
Species Specific

Introduction. The detection of the first cases of tick-borne human granulocytic anaplasmosis in Russia, discovery of genetic markers for Anaplasma spp. in ixodid ticks and reporting of a significant number of cases of tick-borne infections in the southern part of Western Siberia give reason to suppose that causative agents of tick-borne anaplasmosis may be transmitted in Tomsk and its suburbs. Objective. To study the distribution and species biodiversity of A. phagocytophilum in ixodid ticks in Tomsk Region. Materials and methods. The analysis of 690 individual ixodid ticks (larvae and adults) was carried out for Ixodes persulcatus (n = 530) and Dermacentor reticulatus (n = 160) ticks collected in 2015–2016 on the territory of urban and suburban biotopes of Tomsk. Primary screening of ticks for the presence of genetic material of A. phagocytophilum was conducted using two-round PCR with species-specific primers for the 16S rRNA gene. The amplification (1,220 kB) of the groESL fragment of the heat shock protein operon was performed for positive isolates with subsequent determination of the nucleotide sequence in the gene fragment for phylogenetic analysis. Results. The number of A. phagocytophilum positive samples for I. persulcatus (larvae) was 1.2 ± 0.6%, I. persulcatus (adult) was 1.8 ± 0.7%; and D. reticulatus (adult) was 0.6 ± 0.3%. Analysis of the nucleotide sequence of the gene fragments in groESL operon for nine isolates confirmed that the genetic material of the granulocytic anaplasmosis was detected. Phylogenetic analysis showed that all the isolates belonged to the first group of the “new cluster” of A. phagocytophilum. Conclusion. The causative agent of human granulocytic anaplasmosis has been newly detected in I. persulcatus ticks collected in urban and suburban biotopes of Tomsk and in D. reticulatus from urban foci.

scholarly journals The study of anaplasma and ehrlichia genetic variability in parasitic systems in the South-Western Siberia and Ural

2006 ◽  
Vol 5 ◽  
pp. 116-120
Author(s):  
V. A. Rar ◽  
N. N. Livanova ◽  
V. V. Panov ◽  
V. B. Astanin ◽  
S. G. Livanov ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Small Mammals ◽  
Genetic Variants ◽  
Nested Pcr ◽  
Anaplasma Phagocytophilum ◽  
Western Siberia ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Novosibirsk Region ◽  
Ehrlichia Muris

Ehrlichia muris and Anaplasma phagocytophilum DNA were detected using nested PCR among Ixodes persulcatus collected in Novosibirsk, Sverdlovsk and Chelyabinsk regions and in blood samples of small mammals from Novosibirsk and Sverdlovsk re- gions. Two genetic variants of A. phagocytophilum were revealed in blood samples and ticks from Novosibirsk region.

scholarly journals Diversity Borrelia burgdorferi sensu lato in natural foci of Novosibirsk region

2006 ◽  
Vol 5 ◽  
pp. 93-98
Author(s):  
N. V. Fomenko ◽  
Ye. V. Romanova ◽  
Yu. Yu. Karavaeva ◽  
V. V. Panov ◽  
N. Ya. Chernousova ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Small Mammals ◽  
Human Blood ◽  
Test Sample ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Ixodes Persulcatus ◽  
Novosibirsk Region ◽  
Natural Foci ◽  
Pcr Assays

PCR assays were used to test sample from Ixodes persulcatus, blood and tissues of small mammals, human blood after tick bi- tes, as well as isolates from adult ticks. It was demonstrated the presence of two Borrelia species: B. garinii and B. afzelii. Mainly DNA B. garinii NT29 were determined.

scholarly journals The efficacy of FOS and Fitoverm drugs against Ixodes ticks

2021 ◽  
Vol 15 (1) ◽  
pp. 85-91
Author(s):  
E. I. Butakov ◽  
L. D. Shamanskaya
Keyword(s):  
Lethal Effect ◽  
Ixodid Tick ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Biologically Active ◽  
Ixodes Persulcatus ◽  
Field Environment ◽  
Ixodes Ticks ◽  
Working Solution ◽  
Grass Stand

The purpose of the research is studying the efficacy of FOS and Fitoverm drugs against ixodid ticks.Materials and methods. The study objects were ixodid tick species Ixodes persulcatus (Schulze, 1930), Dermacentor reticulatus (Hermann, 1804) and D. marginatus (Schulzer, 1776) in the imago stage. Ticks were collected in the environment of the Altai plains and mountains from vegetation to the flag under the methodology. The efficacy of drug acaricidal activity was assessed according to the methodological guide. In the laboratory, the ticks were placed in a chamber at a temperature of 7 оC. The effect of drugs was assessed by observation every hour for 2-3 days. Live ticks (20 individuals) were contacted with the drug by immersion three times in the working solution in a permeable fabric container. Neostomosan was used as control. Further tests of FOS and Fitoverm were carried out in the field environment. Briz 25% e.c. was used as control. The plots on which the number of Ixodes had previously been recorded was treated using a sprayer on June 1-2 in the morning. The number of live ticks in the plots was counted 1-2 days after the treatment. The biochemical composition of grass stand on various treated grounds was studied according to standard methods. The results were processed statistically.Results and discussion. The Siberian Scientific-Research Institute of Horticulture has developed a series of environmentally friendly drugs based on natural biologically active substances that have shown high efficacy against Ixodes. These are FOS and Fitoverm, which provided a 100% lethal effect against Ixodes in working concentrations of 3–4% and 0.1–0.3% under laboratory conditions. Treatment with Fitoverm 0.3%, FOS 3% and synthetic industrial drug Briz 25% of e.c. 0.75% of grass stand at the stage of grazing for cattle in pasture conditions statistically significantly reduces the number of ticks by 71–79%.

scholarly journals Atlas of ticks (Acari: Argasidae, Ixodidae) in Germany

2021 ◽  
Author(s):  
Franz Rubel ◽  
Katharina Brugger ◽  
Lidia Chitimia-Dobler ◽  
Hans Dautel ◽  
Elisabeth Meyer-Kayser ◽  
...  
Keyword(s):  
Tick Species ◽  
Migratory Birds ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Federal States ◽  
Ixodes Frontalis ◽  
Argas Reflexus ◽  
The One ◽  
Argasid Tick ◽  
First Time

AbstractAn updated and increased compilation of georeferenced tick locations in Germany is presented here. This data collection extends the dataset published some years ago by another 1448 new tick locations, 900 locations of which were digitized from literature and 548 locations are published here for the first time. This means that a total of 3492 georeferenced tick locations is now available for Germany. The tick fauna of Germany includes two species of Argasidae in the genera Argas and Carios and 19 species of Ixodidae in the genera Dermacentor, Haemaphysalis, and Ixodes, altogether 21 tick species. In addition, three species of Ixodidae in the genera Hyalomma (each spring imported by migratory birds) and Rhipicephalus (occasionally imported by dogs returning from abroad with their owners) are included in the tick atlas. Of these, the georeferenced locations of 23 tick species are depicted in maps. The occurrence of the one remaining tick species, the recently described Ixodes inopinatus, is given at the level of the federal states. The most common and widespread tick species is Ixodes ricinus, with records in all 16 federal states. With the exception of Hamburg, Dermacentor reticulatus was also found in all federal states. The occurrence of the ixodid ticks Ixodes canisuga, Ixodes frontalis, Ixodes hexagonus and I. inopinatus were documented in at least 11 federal states each. The two mentioned argasid tick species were also documented in numerous federal states, the pigeon tick Argas reflexus in 11 and the bat tick Carios vespertilionis in seven federal states. The atlas of ticks in Germany and the underlying digital dataset in the supplement can be used to improve global tick maps or to study the effects of climate change and habitat alteration on the distribution of tick species.

Molecular detection of equine infectious anemia virus in clinically normal, seronegative horses in an endemic area of Mexico

2021 ◽  
pp. 104063872110061
Author(s):  
César I. Romo-Sáenz ◽  
Patricia Tamez-Guerra ◽  
Aymee Olivas-Holguin ◽  
Yareellys Ramos-Zayas ◽  
Nelson Obregón-Macías ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Equine Infectious Anemia Virus ◽  
Antibody Detection ◽  
Economic Losses ◽  
Clinically Normal ◽  
Pcr Product ◽  
Surveillance Programs ◽  
Equine Infectious Anemia ◽  
Anemia Virus ◽  
Equine Industry

Equine infectious anemia (EIA) is a highly infectious disease in members of the Equidae family, caused by equine infectious anemia virus (EIAV). The disease severity ranges from subclinical to acute or chronic, and causes significant economic losses in the equine industry worldwide. Serologic tests for detection of EIAV infection have some concerns given the prolonged seroconversion time. Therefore, molecular methods are needed to improve surveillance programs for this disease. We attempted detection of EIAV in 6 clinical and 42 non-clinical horses in Nuevo Leon State, Mexico, using the agar gel immunodiffusion (AGID) test for antibody detection, and nested and hemi-nested PCR for detection of proviral DNA. We found that 6 of 6, 5 of 6, and 6 of 6 clinical horses were positive by AGID, nested PCR, and hemi-nested PCR, respectively, whereas 0 of 42, 1 of 42, and 9 of 42 non-clinical horses were positive by these tests, respectively. BLAST analysis of the 203-bp 5′-LTR/ tat segment of PCR product revealed 83–93% identity with EIAV isolates in GenBank and reference strains from other countries. By phylogenetic analysis, our Mexican samples were grouped in a different clade than other sequences reported worldwide, indicating that the LRT/ tat region represents an important target for the detection of non-clinical horses.

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