scholarly journals The study of anaplasma and ehrlichia genetic variability in parasitic systems in the South-Western Siberia and Ural

2006 ◽  
Vol 5 ◽  
pp. 116-120
Author(s):  
V. A. Rar ◽  
N. N. Livanova ◽  
V. V. Panov ◽  
V. B. Astanin ◽  
S. G. Livanov ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Small Mammals ◽  
Genetic Variants ◽  
Nested Pcr ◽  
Anaplasma Phagocytophilum ◽  
Western Siberia ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Novosibirsk Region ◽  
Ehrlichia Muris

Ehrlichia muris and Anaplasma phagocytophilum DNA were detected using nested PCR among Ixodes persulcatus collected in Novosibirsk, Sverdlovsk and Chelyabinsk regions and in blood samples of small mammals from Novosibirsk and Sverdlovsk re- gions. Two genetic variants of A. phagocytophilum were revealed in blood samples and ticks from Novosibirsk region.

Genetic variability of Anaplasma phagocytophilum in ticks and voles from Ixodes persulcatus/Ixodes trianguliceps sympatric areas from Western Siberia, Russia

2014 ◽  
Vol 5 (6) ◽  
pp. 854-863 ◽  
Author(s):  
Vera A. Rar ◽  
Tamara I. Epikhina ◽  
Valeriy V. Yakimenko ◽  
Marina G. Malkova ◽  
Aleksey K. Tancev ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Anaplasma Phagocytophilum ◽  
Western Siberia ◽  
Ixodes Persulcatus ◽  
Ixodes Trianguliceps

scholarly journals Выявление различных видов риккетсий у иксодовых клещей, в крови людей и мелких млекопитающих на юге Западной Сибири и на Урале

2006 ◽  
Vol 5 ◽  
pp. 121-125
Author(s):  
Ya. P. Igolkina ◽  
N. V. Fomenko ◽  
N. N. Livanova ◽  
V. B. Astanin ◽  
L. A. Gosteeva ◽  
...  
Keyword(s):  
Small Mammals ◽  
Human Blood ◽  
Nested Pcr ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Rickettsia Helvetica ◽  
Rickettsia Slovaca ◽  
High Prevalence ◽  
Human Blood Samples ◽  
Rickettsia Sibirica

We investigated ticks, human blood samples, specimens of tissue and blood from small mammals using nested PCR. We de- tected high prevalence Rickettsia tarasevichiae and Rickettsia sp. RpA4 in samples of ticks Ixodes persulcatus and Dermacentor re- ticulatus, correspondingly. We observed DNA of pathogenic Rickettsia helvetica and Rickettsia slovaca in I. persulcatus and Der- macentor marginatus and DNA of Rickettsia sibirica in human blood and in blood and liver samples of small mammals.

scholarly journals BABESIA INFECTION OF SMALL MAMMALS FROM SOUTHERN TAIGA OF OMSK REGION

2016 ◽  
Vol 1 (1) ◽  
pp. 59-64
Author(s):  
Боброва ◽  
Oksana Bobrova ◽  
Танцев ◽  
Aleksey Tantsev ◽  
Епихина ◽  
...  
Keyword(s):  
Small Mammals ◽  
Small Mammal ◽  
Nested Pcr ◽  
Southern Taiga ◽  
Babesia Microti ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Genetic Groups ◽  
Tamias Sibiricus ◽  
Babesia Spp

Blood samples were taken from 541 small mammal captured in 2013–2015 in Znamensky district of Omsk region from Ixodes persulcatus and Ixodes trianguliceps sympatric area and examined for the Babesia spp. presence by nested PCR with subsequent sequencing of positive samples. Babesia microti DNA was found in 31,1 % of positive samples; a proportion of infected mammals varied from 5,3 % to 61,6 % in different sampling periods. B. microti DNA was found in samples from three prevailing Myodes species as well as from a root vole (Microtus oeconomus), field voles (Microtus argestis) and Siberian chipmunks (Tamias sibiricus). It was shown that identified B. microti samples belong to two genetic groups: B. microti ‘US’-type and B. microti ‘Munich’-type; notably that > 90 % infected mammals contained DNA of nonpathogenic for human B. microti ‘Munich’-type. We suppose that I. trianguliceps tick is the most probable vector of B. microti ‘Munich’-type.

Genetic diversity ofBabesiainIxodes persulcatusand small mammals from North Ural and West Siberia, Russia

Parasitology ◽  
2010 ◽  
Vol 138 (2) ◽  
pp. 175-182 ◽  
Author(s):  
V. A. RAR ◽  
T. I. EPIKHINA ◽  
N. N. LIVANOVA ◽  
V. V. PANOV
Keyword(s):  
Genetic Diversity ◽  
Small Mammals ◽  
West Siberia ◽  
18S Rrna Gene ◽  
Sensu Stricto ◽  
Babesia Microti ◽  
Rrna Gene ◽  
Ixodes Persulcatus ◽  
Novosibirsk Region ◽  
The Us

SUMMARYObjective.The aim of this work was to study the prevalence and genetic diversity ofBabesiainIxodes persulcatusticks and small mammals from Ural and Siberia in Russia.Methods.In total, 481 small mammals and 922 questing adultI. persulcatusfrom North Ural (Sverdlovsk region) and West Siberia (Novosibirsk region) were examined for the presence ofBabesiaby nested PCR based on the 18S rRNA gene.Results.Babesia microtiof the ‘Munich’-type was found in 36·2% of blood samples of the small mammals from the Sverdlovsk region andB. microtiof the ‘US’-type in 5·3% of the animals from the Novosibirsk region.BabesiaDNA was not detected in 133 analysedI. persulcatusfrom the Sverdlovsk region; however, it was found in 24 of 789 ticks from the Novosibirsk region. Three distinctBabesiaspecies were detected inI. persulcatus. B. microti‘US’-type was identified in 10 ticks,Babesiaclosely related toB. divergens/B. capreoliin 2 ticks, andBabesiaclosely related toB. venatorum(EU1) in 12 ticks.Conclusion.To our knowledge, this is the first detection ofBabesia sensu strictoinI. persulcatusticks and ofB. microtiinI. persulcatusin the Asian part of Russia.

Genetic variability of Anaplasmataceae circulating in small mammals and ticks in an Ixodes persulcatus/Ixodes trianguliceps sympatric area in Russian Siberia

2020 ◽  
Vol 11 (5) ◽  
pp. 101499 ◽  
Author(s):  
Vera Rar ◽  
Valeriy Yakimenko ◽  
Artem Tikunov ◽  
Marat Makenov ◽  
Tamara Epikhina ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Small Mammals ◽  
Ixodes Persulcatus ◽  
Ixodes Trianguliceps

scholarly journals DETECTION OF BABESIA AND ANAPLASMATACEAE BACTERIA IN SMALL MAMMALS FROM IRKUTSK AND NOVOSIBIRSK REGIONS

2016 ◽  
Vol 1 (3) ◽  
pp. 37-41
Author(s):  
Козлова ◽  
Irina Kozlova ◽  
Лисак ◽  
Oksana Lisak ◽  
Панов ◽  
...  
Keyword(s):  
Small Mammals ◽  
Babesia Microti ◽  
Ixodes Persulcatus ◽  
Small Rodents ◽  
Tissue Samples ◽  
Candidatus Neoehrlichia Mikurensis ◽  
Novosibirsk Region ◽  
Irkutsk Region ◽  
Pcr Method ◽  
Neoehrlichia Mikurensis

Tissue samples from 228 small mammals captured from 2013 to 2015 in four districts of Irkutsk region and in one district of Novosibirsk region were examined for the presence of Anaplasmataceae bacteria and Babesia parasites by nested PCR method with subsequent sequencing of positive samples. In Ekhirit-Bulagatskiy District of Irkutsk region, Babesia microti DNA was found in 10.9 % of small mammals, Anaplasma phagocytophilum DNA – in 7.3 %, and Ehrlichia muris DNA – in 1.8 %. Infected mammals were not found in the other three examined districts of Irkutsk region. In Novosibirsk region, B. microti DNA was found in 8.9 % of small rodents, A. phagocytophilum DNA – in 8.9 % of small mammals, and Candidatus Neoehrlichia mikurensis DNA – in 8.9 % of small mammals. The identified infection agents corresponded to microorganism species and genetic variants previously found in Ixodes persulcatus ticks but not in ticks of other species.

scholarly journals Concurrent occurrence of Anaplasma phagocytophilum and A. marginale in bovine peripheral blood samples from southwest of Iran

2020 ◽  
Vol 71 (3) ◽  
pp. 2301
Author(s):  
S. BAHRAMI ◽  
H. HAMIDINEJAT ◽  
MR HAJI HAJIKOLAEI ◽  
S. KAVIANIFAR
Keyword(s):  
Nested Pcr ◽  
Anaplasma Phagocytophilum ◽  
Epidemiological Studies ◽  
Infected Cattle ◽  
Blood Samples ◽  
Khuzestan Province ◽  
Pcr Product ◽  
Health Significance ◽  
Cattle Herds ◽  
Southwest Of Iran

Anaplasma phagocytophilum and A. marginale are the most important tick-borne bacteria of veterinary and public health significance. Anaplasma phagocytophilum causes febrile disease in humans (human granulocytic-HGA) and animals. Anaplasma marginale is the aetiological agent of acute anaplasmosis, a bovine syndrome characterized by progressive hemolytic anemia associated with fever, weight loss, abortion, decreased milk production, and in some cases, death of the infected cattle. The present study was designed to investigate the prevalence of A. phagocytophilum and A. marginale in cattle from Khuzestan province, southwest of Iran. Samples were collected between March to August 2016. Farmed cattle were selected from the four geographic regions of Khuzestan province with the highest population of cattle herds: Behbahan; Dezful; Shushtar; and Ahvaz. Blood samples were collected from the jugular vein of 200 cattle Species specification was accomplished by specific Nested PCR according to amplification of the 16SrRNA gene. To identify A. marginale, semi-nested PCR product was cut with restriction endonucleases Bst 1107 I. The prevalence of the A. marginale infection (21.5 %) was higher than that of A. phagocytophilum (7.5 %), which was found in a mixed infection with A. marginale. Overall, in the present study 7.5% of cattle were infected with both A. phagocytophilum and A. marginale. Despite the healthy appearance of infected cattle, they can transmit Anaplasma to ticks and are potential continuous sources for maintaining and disseminating the organisms to the human and animals’ population. More epidemiological studies are needed to determine the vectors and reservoir animals for the Anaplasma species and to clarify the pathogenicity of A. marginale and A. phagocytophilum for humans and animals in Iran.

scholarly journals Detection of bartonella DNA in ixodid ticks, mosquitoes and in patients blood in Novosibirsk region

2006 ◽  
Vol 5 ◽  
pp. 106-110
Author(s):  
O. V. Morozova ◽  
L. V. Petrozhitskaya ◽  
N. Ya. Chernousova ◽  
A. G. Mirzayeva ◽  
A. K. Dobrotvorsky ◽  
...  
Keyword(s):  
Human Blood ◽  
Mixed Infection ◽  
Nested Pcr ◽  
Bartonella Henselae ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Ixodes Persulcatus ◽  
Bartonella Quintana ◽  
Novosibirsk Region ◽  
Pcr Product

Bartonella DNA was detected using nested PCR in ticks Ixodes persulcatus and Dermacentor reticulatus, in mosquitoes Aedes cantans, but not in other mosquito or gnat species. Phylogenic analysis of the PCR product nucleotide sequences proved the infec- tion of arthropod vectors and human blood with Bartonella henselae, Bartonella quintana or their mixed infection in Novosibirsk region.

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