scholarly journals Detection and genotyping of Anaplasma phagocytophilum in I. persulcatus and D. reticulatus ticks collected in Tomsk (Western Siberia) in 2015–2016

2019 ◽  
Vol 18 (2) ◽  
pp. 89-98
Author(s):  
M. Yu. Kartashov ◽  
T. P. Mikryukova ◽  
N. S. Moskvitina ◽  
E. I. Krivosheina ◽  
A. I. Kuznetsov ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Nucleotide Sequence ◽  
Western Siberia ◽  
Genetic Material ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Ixodes Persulcatus ◽  
Human Granulocytic Anaplasmosis ◽  
Granulocytic Anaplasmosis ◽  
Species Specific

Introduction. The detection of the first cases of tick-borne human granulocytic anaplasmosis in Russia, discovery of genetic markers for Anaplasma spp. in ixodid ticks and reporting of a significant number of cases of tick-borne infections in the southern part of Western Siberia give reason to suppose that causative agents of tick-borne anaplasmosis may be transmitted in Tomsk and its suburbs. Objective. To study the distribution and species biodiversity of A. phagocytophilum in ixodid ticks in Tomsk Region. Materials and methods. The analysis of 690 individual ixodid ticks (larvae and adults) was carried out for Ixodes persulcatus (n = 530) and Dermacentor reticulatus (n = 160) ticks collected in 2015–2016 on the territory of urban and suburban biotopes of Tomsk. Primary screening of ticks for the presence of genetic material of A. phagocytophilum was conducted using two-round PCR with species-specific primers for the 16S rRNA gene. The amplification (1,220 kB) of the groESL fragment of the heat shock protein operon was performed for positive isolates with subsequent determination of the nucleotide sequence in the gene fragment for phylogenetic analysis. Results. The number of A. phagocytophilum positive samples for I. persulcatus (larvae) was 1.2 ± 0.6%, I. persulcatus (adult) was 1.8 ± 0.7%; and D. reticulatus (adult) was 0.6 ± 0.3%. Analysis of the nucleotide sequence of the gene fragments in groESL operon for nine isolates confirmed that the genetic material of the granulocytic anaplasmosis was detected. Phylogenetic analysis showed that all the isolates belonged to the first group of the “new cluster” of A. phagocytophilum. Conclusion. The causative agent of human granulocytic anaplasmosis has been newly detected in I. persulcatus ticks collected in urban and suburban biotopes of Tomsk and in D. reticulatus from urban foci.

scholarly journals INFLUENCE OF NATURAL FACTORS ON THE NUMBER OF IXODIC TICKS OF THE GENUS DERMACENTOR IN TYUMEN REGION

2021 ◽  
pp. 156-160
Author(s):  
Glazunov
Keyword(s):  
Air Temperature ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Ixodes Persulcatus ◽  
Large Mammals ◽  
Depressing Effect ◽  
Dominance Index ◽  
Spring Period ◽  
Natural Factors ◽  
Ixodes Apronophorus

At present, the ixodofauna of the Tyumen region is represented by six species of ixodid ticks – these are Ixodes persulcatus Schulze, 1930; Dermacentor reticulatus Fabricius, 1794 (D. pictus, Hermann, 1804); D. marginatus Sulz, 1776; I. apronophorus P. Sch., 1924; I. (Exopalpiger) trianguliceps Bir., 1895 and I. (Ceratixodes) plumbeus, Kirsch., 1936. Three species of ixodid ticks parasitize cattle: I. persulcatus, D. reticulatus, D. marginatus. The remaining three species of ticks, Ixodes apronophorus, Ixodes strianguliceps, and Ixodes plumbeus, belong to the nest-like parasitic type and therefore are less dangerous parasites for large mammals. The most widespread ticks of the genus Ixodes and Dermacentor in the context of all natural-geographical zones of the region are ticks of the genus Dermacentor, their dominance index was 54.8%. As a result of observation, it was noted that the temperature and humidity of the air affect the activity of ixodid ticks of the genus Dermacentor in nature. Thus, the peak of ixodid activity in the spring period fell on days when the air temperature reached values from 16 oC to 26 oC, and in autumn from 15 oC to 21 oC, while lower and higher humidity indices had a depressing effect on ixodid mites of the genus Dermacentor.

scholarly journals Dynamics of tick Infection with borrelia, anaplasma and Ehrlichia in the kirov Region

2020 ◽  
Vol 14 (2) ◽  
pp. 38-45
Author(s):  
I. A. Novoselova ◽  
E. A. Bessolitsyna
Keyword(s):  
Genetic Material ◽  
Maximum Level ◽  
Dermacentor Reticulatus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Ixodes Persulcatus ◽  
Tick Infection ◽  
Simultaneous Infection ◽  
Combined Infection ◽  
And Gender ◽  
Research Period

The purpose of the research is identification of the genetic material of microorganisms of the group Borrelia burgdorferi sensu lato, of the genera Anaplasma and Ehrlichia in ixodic ticks of various species collected in the Kirov Region from 2010 to 2017. Materials and methods. Ixodic ticks were collected from the vegetation cover, people's clothing and the hair of domestic animals (dogs and cats). Species and gender of ticks were identified using definition tables. Total nucleic acids were isolated from ixodic ticks fixed in 70 % ethanol by the method using guanidine thioisocyanate buffer. Ticks’ infection by pathogens was determined using polymerase chain reaction. Results and discussion. Three species of ixodic ticks Ixodes persulcatus, Dermacentor reticulatus and I. ricinus were studied. The species I. persulcatus had the highest infection rates with the studied pathogens. The largest percentage of ticks tested was infected with Ehrlichia (35.6%). The most common were cases of simultaneous infection of ticks with Borrelia and Ehrlichia (16.3%). Cases of combined infection with three pathogens at once (Borrelia, Anaplasma and Ehrlichia) were found in all studied tick species with the same probability. Peaks of tick infection were revealed: the maximum level for all studied pathogens during the research period was noted in 2011; an increase in the number of infected ticks was observed in 2015 and 2016. 

scholarly journals Transmissible parasitic zoonoses of the Kaluga Region

2020 ◽  
Vol 14 (4) ◽  
pp. 50-56
Author(s):  
F. I. Vasilevich ◽  
A. M. Nikanorova
Keyword(s):  
Causative Agent ◽  
Mosquito Species ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Abundance Index ◽  
Granulocytic Anaplasmosis ◽  
Blood Sucking ◽  
Parasitic Zoonoses ◽  
Kaluga Region ◽  
The Government

the purpose of the research is monitoring population and species composition of common blood-sucking ectoparasites of the Kaluga Region, mosquitoes and ixodid ticks, and parasitic zoonoses, in the circulation of which they are involved.Materials and methods. The records were made in all districts of the Kaluga Region and in the city of Kaluga. We studied open meadow-field and forest-shrub stations, closed meadow-field and near-water stations, and settlement stations. The species was identified using the atlases of ixodid ticks by I. M. Ganiev, A. A. Aliverdiev (1968) and V. N. Shevkoplyas (2008), and the guidance of R. M. Gornostaeva (1999). The situation with transmissible parasitic zoonoses in the Kaluga Region was assessed based on the analysis of information from open sources of the Hygienic and Epidemiological Center of the Kaluga Region and the Veterinary Committee under the Government of the Kaluga Region.Results and discussion. There are two species of ixodid ticks in the Kaluga region: Ixodes ricinus and Dermacentor reticulatus. The abundance index of ixodid ticks of the species I. ricinus is 16.8±1.32 individuals per 1 flag-hour in forest biotopes, and 11.6±1.12 individuals per 1 flag-hour in meadow biotopes, and the abundance index of D. reticulatus is 10,8±1.14 and 15.9±1.30 individuals per 1 flag-hour, respectively. Three mosquito species prevail, namely, Stegomya communis, S. vexans, and Culex pipiens. The maximum number was 8 thousand per 1 m3. During the study period, the causative agent of infectious tick-borne borreliosis was found on average in 10.6% of ixodid ticks collected from humans and in 13.2% of ticks collected in natural biotopes. The causative agent of human granulocytic anaplasmosis was isolated on average in 3.1% of ixodid ticks collected from humans and 6.4% of those collected in natural biotopes. Infection rate for I. ricinus ticks infected with pathogen of infectious tick-borne borreliosis was 16.9%, and D. reticulatus 12.3%.

scholarly journals Detection of bartonella DNA in ixodid ticks, mosquitoes and in patients blood in Novosibirsk region

2006 ◽  
Vol 5 ◽  
pp. 106-110
Author(s):  
O. V. Morozova ◽  
L. V. Petrozhitskaya ◽  
N. Ya. Chernousova ◽  
A. G. Mirzayeva ◽  
A. K. Dobrotvorsky ◽  
...  
Keyword(s):  
Human Blood ◽  
Mixed Infection ◽  
Nested Pcr ◽  
Bartonella Henselae ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Ixodes Persulcatus ◽  
Bartonella Quintana ◽  
Novosibirsk Region ◽  
Pcr Product

Bartonella DNA was detected using nested PCR in ticks Ixodes persulcatus and Dermacentor reticulatus, in mosquitoes Aedes cantans, but not in other mosquito or gnat species. Phylogenic analysis of the PCR product nucleotide sequences proved the infec- tion of arthropod vectors and human blood with Bartonella henselae, Bartonella quintana or their mixed infection in Novosibirsk region.

scholarly journals Occurrence and Genetic Diversity of Kemerovo Virus in Ixodid Tick of Western Siberia

2017 ◽  
Vol 16 (2) ◽  
pp. 75-79
Author(s):  
S. E. Tkachev ◽  
A. Yu. Tikunov ◽  
I. V. Babkin ◽  
N. N. Livanova ◽  
S. G. Livanov ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Western Siberia ◽  
Ixodid Tick ◽  
Ixodid Ticks ◽  
Distribution Area ◽  
Ixodes Persulcatus ◽  
Northeastern Altai ◽  
Ixodes Ticks ◽  
East Kazakhstan ◽  
First Time

Kemerovo virus (KEMV), a member of Reoviridae family, Orbivirus genus, is transmitted by ixodes ticks and can cause the damage of human central nervous system. The occurrence and genetic diversity of KEMV in Western Siberia still remained poorly studied, so, the aim of this work was to investigate the prevalence and genetic variability of KEMV in ixodid ticks from Western Siberia. A total of 1958 Ixodes persulcatus, I. pavlovskyi ticks and their hybrids from Novosibirsk and Omsk provinces, Altai Republic (Russia) and East Kazakhstan province (Kazakhstan) were analyzed for the presence of KEMV and tick-borne encephalitis virus (TBEV) RNA. It was shown that KEMV distribution area in Western Siberia was wider and included Northern and Northeastern Altai in addition to Omsk and Novosibirsk provinces. For the first time this virus was found in Kazakhstan. The occurrence of KEMV was statistically lower than TBEV in most locations in Western Siberia. KEMV was found both in I. persulcatus and I. pavlovskyi ticks and in their hybrids. Notably, KEMV variants found in 2010s genetically differ from those isolated in 1960s. Moreover, the possibility of reassortment for KEMV was demonstrated for the first time.

scholarly journals Detection of Borrelia miyamotoi in Ixodidae Ticks Collected in the South of Western Siberia

2021 ◽  
pp. 129-133
Author(s):  
N. L. Tupota ◽  
V. A. Ternovoy ◽  
M. Yu. Kartashov ◽  
E. P. Ponomareva ◽  
V. B. Loktev
Keyword(s):  
Western Siberia ◽  
Genetic Material ◽  
Ixodes Persulcatus ◽  
Borrelia Miyamotoi ◽  
The South ◽  
West Nile Fever ◽  
Round Polymerase Chain Reaction ◽  
Tick Borne Encephalitis ◽  
Ixodes Pavlovskyi ◽  
Polymerase Chain

Objective of the study was to assess the level of infection with Borrelia miyamotoi in ticks Ixodes persulcatus and Ixodes pavlovskyi collected in the south of Western Siberia.Materials and methods. 688 ticks I. persulcatus and I. pavlovskyi collected on the territory of Novosibirsk, Tomsk and Kemerovo Regions were examined. Borrelia DNA was detected by a two-round polymerase chain reaction with specifc primers to the omp66 gene region, followed by sequencing of the isolated fragments and phylogenetic analysis, including the sequences of prototype isolates published in the international GenBank database. Comparison and analysis of nucleotide sequences was carried out using the MEGA 7 software package. In parallel, the samples were examined for the presence of the agents of transmissible infections – West Nile fever and tick-borne encephalitis, anaplasmosis, ehrlichiosis, babesiosis, bartonellosis and candidate pathogens of tick-borne rickettsiosis through RT-PCR and PCR with species-specifc and genus-specifc primers.Results and discussion. The genetic material of B. miyamotoi was found in 2.2 % of I. persulcatus and I. pavlovskyi ticks in the Tomsk, Novosibirsk, and Kemerovo Regions and their appurtenance to the Asian genotype was determined. It was revealed that the degree of similarity of the omp66 gene fragments within the group of isolates of the Asian genotype was 100 %. No relation between B. miyamotoi and a specifc vector species was identifed.

scholarly journals The efficacy of FOS and Fitoverm drugs against Ixodes ticks

2021 ◽  
Vol 15 (1) ◽  
pp. 85-91
Author(s):  
E. I. Butakov ◽  
L. D. Shamanskaya
Keyword(s):  
Lethal Effect ◽  
Ixodid Tick ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Biologically Active ◽  
Ixodes Persulcatus ◽  
Field Environment ◽  
Ixodes Ticks ◽  
Working Solution ◽  
Grass Stand

The purpose of the research is studying the efficacy of FOS and Fitoverm drugs against ixodid ticks.Materials and methods. The study objects were ixodid tick species Ixodes persulcatus (Schulze, 1930), Dermacentor reticulatus (Hermann, 1804) and D. marginatus (Schulzer, 1776) in the imago stage. Ticks were collected in the environment of the Altai plains and mountains from vegetation to the flag under the methodology. The efficacy of drug acaricidal activity was assessed according to the methodological guide. In the laboratory, the ticks were placed in a chamber at a temperature of 7 оC. The effect of drugs was assessed by observation every hour for 2-3 days. Live ticks (20 individuals) were contacted with the drug by immersion three times in the working solution in a permeable fabric container. Neostomosan was used as control. Further tests of FOS and Fitoverm were carried out in the field environment. Briz 25% e.c. was used as control. The plots on which the number of Ixodes had previously been recorded was treated using a sprayer on June 1-2 in the morning. The number of live ticks in the plots was counted 1-2 days after the treatment. The biochemical composition of grass stand on various treated grounds was studied according to standard methods. The results were processed statistically.Results and discussion. The Siberian Scientific-Research Institute of Horticulture has developed a series of environmentally friendly drugs based on natural biologically active substances that have shown high efficacy against Ixodes. These are FOS and Fitoverm, which provided a 100% lethal effect against Ixodes in working concentrations of 3–4% and 0.1–0.3% under laboratory conditions. Treatment with Fitoverm 0.3%, FOS 3% and synthetic industrial drug Briz 25% of e.c. 0.75% of grass stand at the stage of grazing for cattle in pasture conditions statistically significantly reduces the number of ticks by 71–79%.

Nucleotide sequence and phylogenetic analysis of the medium (M) genomic RNA segments of three hantaviruses isolated in China

1998 ◽  
Vol 56 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Xiaohong Shi ◽  
Mifang Liang ◽  
Changshou Hang ◽  
Gan Song ◽  
Conall McCaughey ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Nucleotide Sequence ◽  
Genomic Rna

scholarly journals Atlas of ticks (Acari: Argasidae, Ixodidae) in Germany

2021 ◽  
Author(s):  
Franz Rubel ◽  
Katharina Brugger ◽  
Lidia Chitimia-Dobler ◽  
Hans Dautel ◽  
Elisabeth Meyer-Kayser ◽  
...  
Keyword(s):  
Tick Species ◽  
Migratory Birds ◽  
Dermacentor Reticulatus ◽  
Ixodid Ticks ◽  
Federal States ◽  
Ixodes Frontalis ◽  
Argas Reflexus ◽  
The One ◽  
Argasid Tick ◽  
First Time

AbstractAn updated and increased compilation of georeferenced tick locations in Germany is presented here. This data collection extends the dataset published some years ago by another 1448 new tick locations, 900 locations of which were digitized from literature and 548 locations are published here for the first time. This means that a total of 3492 georeferenced tick locations is now available for Germany. The tick fauna of Germany includes two species of Argasidae in the genera Argas and Carios and 19 species of Ixodidae in the genera Dermacentor, Haemaphysalis, and Ixodes, altogether 21 tick species. In addition, three species of Ixodidae in the genera Hyalomma (each spring imported by migratory birds) and Rhipicephalus (occasionally imported by dogs returning from abroad with their owners) are included in the tick atlas. Of these, the georeferenced locations of 23 tick species are depicted in maps. The occurrence of the one remaining tick species, the recently described Ixodes inopinatus, is given at the level of the federal states. The most common and widespread tick species is Ixodes ricinus, with records in all 16 federal states. With the exception of Hamburg, Dermacentor reticulatus was also found in all federal states. The occurrence of the ixodid ticks Ixodes canisuga, Ixodes frontalis, Ixodes hexagonus and I. inopinatus were documented in at least 11 federal states each. The two mentioned argasid tick species were also documented in numerous federal states, the pigeon tick Argas reflexus in 11 and the bat tick Carios vespertilionis in seven federal states. The atlas of ticks in Germany and the underlying digital dataset in the supplement can be used to improve global tick maps or to study the effects of climate change and habitat alteration on the distribution of tick species.

Sign in / Sign up

Export Citation Format

Share Document